##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937107_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3160490 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.398138263370555 31.0 31.0 34.0 30.0 34.0 2 31.485538951238574 33.0 31.0 34.0 28.0 34.0 3 31.491842404184162 33.0 31.0 34.0 28.0 34.0 4 35.074129960860496 37.0 35.0 37.0 32.0 37.0 5 35.097173539546084 37.0 35.0 37.0 32.0 37.0 6 35.143156915541574 37.0 35.0 37.0 32.0 37.0 7 35.06443874209379 37.0 35.0 37.0 32.0 37.0 8 35.029534027951364 37.0 35.0 37.0 32.0 37.0 9 36.58577847105987 39.0 37.0 39.0 32.0 39.0 10-11 36.43834199760164 39.0 37.0 39.0 32.0 39.0 12-13 36.312151913152704 39.0 37.0 39.0 32.0 39.0 14-15 37.38075693958848 40.0 37.0 41.0 32.0 41.0 16-17 37.30614730627213 40.0 37.0 41.0 32.0 41.0 18-19 37.2464606754016 40.0 37.0 41.0 31.0 41.0 20-21 37.17374805805429 40.0 37.0 41.0 31.0 41.0 22-23 37.02052339985256 39.0 36.0 41.0 31.0 41.0 24-25 36.82675914177865 39.0 36.0 41.0 30.0 41.0 26-27 36.786136801571914 39.0 36.0 41.0 30.0 41.0 28-29 36.86643590076223 39.0 36.0 41.0 30.0 41.0 30-31 36.745630107989584 39.0 36.0 41.0 30.0 41.0 32-33 36.566407424165234 39.0 36.0 41.0 30.0 41.0 34-35 36.361481605700384 39.0 35.0 41.0 30.0 41.0 36-37 36.20061193042851 39.0 35.0 41.0 29.5 41.0 38-39 36.03483716132625 39.0 35.0 40.5 29.0 41.0 40-41 35.834185363661966 39.0 35.0 40.0 27.5 41.0 42-43 35.62398061692966 38.0 35.0 40.0 27.0 41.0 44-45 35.3841007248876 38.0 35.0 40.0 26.5 41.0 46-47 35.05048077987907 38.0 34.0 40.0 25.5 41.0 48-49 34.720563741698285 38.0 34.0 40.0 24.0 41.0 50-51 34.060884071773685 37.5 33.0 39.5 23.5 40.5 52-53 34.378749972314424 38.0 33.5 40.0 24.0 40.5 54-55 34.68334467123769 38.0 34.0 40.0 24.0 41.0 56-57 34.54783815167902 38.0 34.0 40.0 23.5 41.0 58-59 34.2918303807321 38.0 34.0 40.0 23.0 41.0 60-61 33.98235732433895 38.0 33.0 40.0 22.5 41.0 62-63 33.66941471101 37.0 33.0 40.0 22.0 41.0 64-65 33.35010567981547 37.0 33.0 39.5 21.5 41.0 66-67 32.955365149074986 36.0 32.0 39.0 20.0 41.0 68-69 32.50309667171862 36.0 32.0 39.0 19.0 40.5 70-71 32.13164461839778 35.0 31.0 38.5 18.0 40.0 72-73 31.739992374600142 35.0 31.0 37.5 16.5 40.0 74-75 31.314809887074475 35.0 31.0 37.0 13.0 39.0 76-77 30.876601254868707 35.0 30.5 36.5 9.5 39.0 78-79 30.43706181636392 34.5 30.0 36.0 7.0 39.0 80-81 30.00087834481362 34.0 30.0 36.0 6.5 37.5 82-83 29.57548449765701 34.0 29.0 35.0 2.0 37.0 84-85 29.215580653632824 34.0 29.0 35.0 2.0 36.5 86-87 28.895990969754692 34.0 29.0 35.0 2.0 36.0 88-89 28.511784406848307 34.0 29.0 35.0 2.0 36.0 90-91 28.154008713838678 34.0 27.5 35.0 2.0 35.5 92-93 27.764509617179613 34.0 27.0 35.0 2.0 35.0 94-95 27.33383225385937 33.5 26.0 35.0 2.0 35.0 96-97 26.851372730177914 33.0 25.5 35.0 2.0 35.0 98-99 26.206722058921244 33.0 24.5 35.0 2.0 35.0 100-101 23.97860442526317 31.0 11.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5561629951731177 1101 2 0.5096251370722591 1101 3 0.6575120804958523 1101 4 0.5425819688227591 1101 5 0.4005262993265859 1101 6 0.4709566578374691 1101 7 0.5460195493709534 1101 8 0.47701076068543813 1101 9 0.6222792105981014 1101 10-11 0.584000192839575 1101 12-13 0.7106213396079966 1101 14-15 0.983645918545669 1101 16-17 0.9267119431764712 1101 18-19 1.016813422358787 1101 20-21 0.9437347199761277 1101 22-23 0.9518892839473025 1101 24-25 0.9937100625395274 1101 26-27 1.1526384559211564 1101 28-29 1.0745019354418375 1101 30-31 0.9932150212339153 1101 32-33 1.0299033853425499 1101 34-35 0.9673544320340142 1101 36-37 0.9314281179827546 1101 38-39 1.0845588957908703 1101 40-41 1.126337701094009 1101 42-43 1.1015587276409349 1101 44-45 1.1231796280748938 1101 46-47 1.1635892593424089 1101 48-49 1.0813987223023673 1101 50-51 1.131696172622398 1101 52-53 1.2871714768089078 1101 54-55 1.2660730381508216 1101 56-57 1.2610224108188675 1101 58-59 1.2304541759975294 1101 60-61 1.2593797632335324 1101 62-63 1.3238737047415654 1101 64-65 1.1976593810917393 1101 66-67 1.1678587785231969 1101 68-69 1.2645781973959487 1101 70-71 1.2523668973041922 1101 72-73 1.4076055864021733 1101 74-75 1.416703148140794 1101 76-77 1.402405038742092 1101 78-79 1.4141426871775913 1101 80-81 1.4171245183411756 1101 82-83 1.177216548441475 1101 84-85 1.1759967090989996 1101 86-87 1.3691944376850707 1101 88-89 1.5088523258530344 1101 90-91 1.626454874077691 1101 92-93 1.3667325232075065 1101 94-95 1.3784886580530973 1101 96-97 1.430156436873471 1101 98-99 1.4032127256995786 1101 100-101 1.7767010878628824 1102 1 0.49892682456513526 1102 2 0.4944258754824169 1102 3 0.43878488053721654 1102 4 0.4203009784799221 1102 5 0.2644703976944598 1102 6 0.3089286725520566 1102 7 0.3957831470523132 1102 8 0.33097176818635177 1102 9 0.3850239669476423 1102 10-11 0.5920458187743165 1102 12-13 0.46075778080040664 1102 14-15 0.5862590785619872 1102 16-17 0.5729403256254457 1102 18-19 0.5412805357227413 1102 20-21 0.632537254622612 1102 22-23 0.5825606895118085 1102 24-25 0.6419063578499689 1102 26-27 0.5788525910371902 1102 28-29 0.6471287683018545 1102 30-31 0.6487311710836821 1102 32-33 0.6836774923469164 1102 34-35 0.6217599027034737 1102 36-37 0.579567155866485 1102 38-39 0.5652022471312463 1102 40-41 0.7895301760510591 1102 42-43 0.7138796066860564 1102 44-45 0.835144419544882 1102 46-47 0.7260998155820317 1102 48-49 0.8910826957762836 1102 50-51 0.6626507056296802 1102 52-53 0.708313829495502 1102 54-55 0.7508260939547853 1102 56-57 0.9216409825237477 1102 58-59 0.7629869266711253 1102 60-61 0.8510793513247492 1102 62-63 0.9004137667480947 1102 64-65 1.0348847513021724 1102 66-67 0.7585230412715944 1102 68-69 0.8620280396986573 1102 70-71 1.0966559956050475 1102 72-73 0.9812493609496151 1102 74-75 0.961196477363341 1102 76-77 1.092607220652198 1102 78-79 1.079948992388399 1102 80-81 0.9752029859103004 1102 82-83 0.8585775325798259 1102 84-85 1.0057456764546089 1102 86-87 1.0354605257772498 1102 88-89 1.0676808848616979 1102 90-91 0.9091201402672731 1102 92-93 0.9603266810350171 1102 94-95 1.074459094837259 1102 96-97 1.1088605998139371 1102 98-99 1.2472614960053825 1102 100-101 1.4806366263412123 1103 1 0.34720542215064043 1103 2 0.40270490187862507 1103 3 0.33573858024689684 1103 4 0.3423615447208235 1103 5 0.2631474440027475 1103 6 0.2055162478710244 1103 7 0.22711100299517994 1103 8 0.24819767547529636 1103 9 0.40653082228617166 1103 10-11 0.4677383208011676 1103 12-13 0.25816543841468587 1103 14-15 0.39219080245005244 1103 16-17 0.5684830564300754 1103 18-19 0.5704171326446854 1103 20-21 0.46214069194011387 1103 22-23 0.4775639364809692 1103 24-25 0.7061384759895049 1103 26-27 0.6068788226233153 1103 28-29 0.6746981166222383 1103 30-31 0.7289289473004601 1103 32-33 0.6703261259488826 1103 34-35 0.6096310102015892 1103 36-37 0.5478952156761352 1103 38-39 0.6999229405544867 1103 40-41 0.7549185204674558 1103 42-43 0.713131459202792 1103 44-45 0.8197509330340651 1103 46-47 0.7139895472737194 1103 48-49 0.6957995771450811 1103 50-51 0.6212622056785548 1103 52-53 0.7361497309082878 1103 54-55 0.6272739205931686 1103 56-57 0.6871038782242564 1103 58-59 0.7188401382270584 1103 60-61 0.6176507953665933 1103 62-63 0.7172968798556454 1103 64-65 0.760246305951739 1103 66-67 0.6543591076674744 1103 68-69 0.7766130363390289 1103 70-71 0.6068569660981424 1103 72-73 0.5966472264171649 1103 74-75 0.49845522137679765 1103 76-77 0.4991663709092826 1103 78-79 0.5471848568071813 1103 80-81 0.5443150061647017 1103 82-83 0.6259129319570356 1103 84-85 0.5958816857605278 1103 86-87 0.6576249695934173 1103 88-89 0.6415610620695347 1103 90-91 0.6614918631814284 1103 92-93 0.6489536037331654 1103 94-95 0.5911187539618261 1103 96-97 0.44221458631803046 1103 98-99 0.5864139661912269 1103 100-101 1.0080890510658556 1104 1 0.305402849144329 1104 2 0.36902176824114363 1104 3 0.2998350075020184 1104 4 0.3299252056447486 1104 5 0.24759833982284363 1104 6 0.27760309880628853 1104 7 0.35122962932040735 1104 8 0.2855208819461623 1104 9 0.2982635310090487 1104 10-11 0.43335556881884685 1104 12-13 0.40007976944333024 1104 14-15 0.49252905625800025 1104 16-17 0.5477233963050097 1104 18-19 0.5214960826549131 1104 20-21 0.5034178195549757 1104 22-23 0.550563865180024 1104 24-25 0.44022000808716655 1104 26-27 0.5371179432875692 1104 28-29 0.48170570467448215 1104 30-31 0.5585606619604704 1104 32-33 0.5017949927995105 1104 34-35 0.5570265215056267 1104 36-37 0.6185081590323236 1104 38-39 0.6959636097617405 1104 40-41 0.5508695846074474 1104 42-43 0.6662118905699614 1104 44-45 0.5999292350570613 1104 46-47 0.5096514169121846 1104 48-49 0.5047754791995729 1104 50-51 0.7209804782659646 1104 52-53 0.8465976427306643 1104 54-55 0.8400052540236942 1104 56-57 0.928277903254795 1104 58-59 0.8297670256712522 1104 60-61 0.8256858442148314 1104 62-63 0.8442016526557197 1104 64-65 0.8194203253065737 1104 66-67 0.8031375735052819 1104 68-69 0.7503390817935625 1104 70-71 0.710629992135047 1104 72-73 0.7589415666475325 1104 74-75 0.6493609207344164 1104 76-77 0.7135488000863397 1104 78-79 0.7294711304531099 1104 80-81 0.6513168830467713 1104 82-83 0.5694604980230302 1104 84-85 0.7403196622752013 1104 86-87 0.7617756068500405 1104 88-89 0.6544253841766974 1104 90-91 0.6087118712905841 1104 92-93 0.5950813062074509 1104 94-95 0.5667911200925282 1104 96-97 0.6019722446956948 1104 98-99 0.6197422800799792 1104 100-101 0.7633777365283052 1105 1 0.28600614572100014 1105 2 0.3631811801206588 1105 3 0.3614606811670349 1105 4 0.3142754257693383 1105 5 0.3011635830270123 1105 6 0.20494164992053499 1105 7 0.3551248030555527 1105 8 0.24825012444332373 1105 9 0.4007331740458824 1105 10-11 0.4249141718745406 1105 12-13 0.2989273892804931 1105 14-15 0.31158818201291183 1105 16-17 0.5598057431141612 1105 18-19 0.5139140327806331 1105 20-21 0.5871370291816049 1105 22-23 0.41821238084457235 1105 24-25 0.6471340805059071 1105 26-27 0.5128640040993488 1105 28-29 0.43081739407138997 1105 30-31 0.3405819203073648 1105 32-33 0.3530529555986206 1105 34-35 0.20122407348313232 1105 36-37 0.30952917701578997 1105 38-39 0.29819210727129075 1105 40-41 0.4890058503399146 1105 42-43 0.3333008008824194 1105 44-45 0.1697019777741673 1105 46-47 0.35956139793859165 1105 48-49 0.4812827777839672 1105 50-51 0.19119804975409949 1105 52-53 0.26001236420018614 1105 54-55 0.41667345533180367 1105 56-57 0.4915726950154209 1105 58-59 0.5592887736956413 1105 60-61 0.3732716342345128 1105 62-63 0.2808146607493427 1105 64-65 0.38619695433368406 1105 66-67 0.5403542535805101 1105 68-69 0.682616249856963 1105 70-71 0.46907729823102073 1105 72-73 0.48678020579076176 1105 74-75 0.5676040708545038 1105 76-77 0.3373451797576763 1105 78-79 0.48480205424295164 1105 80-81 0.4454183157205911 1105 82-83 0.5053052729512686 1105 84-85 0.5320262808900402 1105 86-87 0.7334714655948993 1105 88-89 0.6382406522775135 1105 90-91 0.5142010734456157 1105 92-93 0.6404812315088257 1105 94-95 0.7466509237314192 1105 96-97 0.7888855332828477 1105 98-99 0.9243060395656855 1105 100-101 0.9934453858868899 1106 1 0.525960513654951 1106 2 0.48563430581569733 1106 3 0.5012711020885092 1106 4 0.4005449939368333 1106 5 0.40336710003087006 1106 6 0.4998221839538033 1106 7 0.6087791289634623 1106 8 0.430373398361958 1106 9 0.5875780465512435 1106 10-11 0.6966307795963118 1106 12-13 0.6204707000503973 1106 14-15 0.8879592471933009 1106 16-17 0.9252206452198521 1106 18-19 0.8927012490870752 1106 20-21 0.8396491307558236 1106 22-23 0.9285185772053026 1106 24-25 1.009572967994771 1106 26-27 0.8874418349315931 1106 28-29 0.9105749936380647 1106 30-31 0.9414248312181357 1106 32-33 1.052719611610982 1106 34-35 1.0327579822188113 1106 36-37 1.0961603341602597 1106 38-39 1.1021844483076322 1106 40-41 0.9883688205518837 1106 42-43 1.1041410836244339 1106 44-45 1.2885318157505452 1106 46-47 0.9999204052211113 1106 48-49 1.1362159339964961 1106 50-51 1.0884164494392863 1106 52-53 1.131053880635541 1106 54-55 1.1644834016464074 1106 56-57 1.2476485213354849 1106 58-59 1.4162895997050882 1106 60-61 1.27468874927537 1106 62-63 1.3853353481174295 1106 64-65 1.1951727623770196 1106 66-67 1.3277181870433594 1106 68-69 1.3872133542428742 1106 70-71 1.327562171749328 1106 72-73 1.2744611603310325 1106 74-75 1.3115979085527947 1106 76-77 1.3795021541786987 1106 78-79 1.3755154280177742 1106 80-81 1.4928612668648178 1106 82-83 1.4900244302148735 1106 84-85 1.5418057527770266 1106 86-87 1.545065483613456 1106 88-89 1.514139348939132 1106 90-91 1.630070498889605 1106 92-93 1.5673256658279833 1106 94-95 1.5956777567558227 1106 96-97 1.6347201513774863 1106 98-99 1.7520434252804158 1106 100-101 1.8330302547293158 1107 1 0.1664251065947191 1107 2 0.35039487320403495 1107 3 0.43158349089796033 1107 4 0.43246424468848943 1107 5 0.18994649927278573 1107 6 0.2101180037685495 1107 7 0.34960050049704705 1107 8 0.5031724927472894 1107 9 0.4144198939580619 1107 10-11 0.43316680918113804 1107 12-13 0.4384499304868896 1107 14-15 0.3834996097545158 1107 16-17 0.505734428431218 1107 18-19 0.40612922059722223 1107 20-21 0.5877834566331117 1107 22-23 0.5421482660626751 1107 24-25 0.5589184908043023 1107 26-27 0.4849651957513714 1107 28-29 0.41181790570061594 1107 30-31 0.5816206671611468 1107 32-33 0.6361835003399747 1107 34-35 0.8675071458379193 1107 36-37 0.6402225664837076 1107 38-39 0.6204532277205814 1107 40-41 0.7171313187733048 1107 42-43 0.7856492249810216 1107 44-45 0.7688915635416436 1107 46-47 0.7441415361414343 1107 48-49 0.807462915170106 1107 50-51 0.6454476434878202 1107 52-53 0.7021338610840573 1107 54-55 0.7813874236264482 1107 56-57 0.831388338347061 1107 58-59 0.8259941826221464 1107 60-61 0.8262040847139289 1107 62-63 0.7968039248726981 1107 64-65 0.9799610498720597 1107 66-67 0.9047899905163064 1107 68-69 0.9931871018276723 1107 70-71 0.9882236226590777 1107 72-73 0.9411651081856256 1107 74-75 1.0041172980949504 1107 76-77 0.8767760207403512 1107 78-79 0.9819176955498605 1107 80-81 0.9888350410451174 1107 82-83 0.8533594314658082 1107 84-85 0.8538910886088154 1107 86-87 0.927306843828756 1107 88-89 0.8264193543114224 1107 90-91 0.8966619572897869 1107 92-93 0.8728302783420396 1107 94-95 0.9489001526719711 1107 96-97 1.1192088975878676 1107 98-99 1.0661964185371282 1107 100-101 1.0716015997148993 1108 1 -0.10904209601249448 1108 2 0.0027301806642725523 1108 3 -0.0190606131866069 1108 4 -0.036472593648490204 1108 5 -0.043979121646330555 1108 6 -0.05227203161917515 1108 7 -0.14811615708378412 1108 8 -0.20437003862112846 1108 9 -0.17876439205118544 1108 10-11 -0.11257492926858248 1108 12-13 -0.29781832597828384 1108 14-15 0.03666217393097071 1108 16-17 -0.06816585117487506 1108 18-19 -0.012679054396130596 1108 20-21 0.06981986731685907 1108 22-23 0.04399353254010663 1108 24-25 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-2.280264543111265 2316 90-91 -2.1455779619272803 2316 92-93 -2.3906903022740096 2316 94-95 -2.424814200060222 2316 96-97 -2.3818987804242155 2316 98-99 -2.6128803740083733 2316 100-101 -2.349066563183044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10345.0 3 3423.0 4 3700.0 5 3976.0 6 4345.0 7 5029.0 8 6294.0 9 7727.0 10 9303.0 11 11396.0 12 13573.0 13 15689.0 14 17366.0 15 20153.0 16 23510.0 17 28582.0 18 26285.0 19 20625.0 20 20608.0 21 22160.0 22 23994.0 23 27138.0 24 31567.0 25 35607.0 26 41339.0 27 49103.0 28 57745.0 29 69035.0 30 82623.0 31 101323.0 32 126657.0 33 160196.0 34 212397.0 35 291940.0 36 420199.0 37 583124.0 38 490781.0 39 81599.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.157405320250945 18.698949677203665 14.310363168038984 22.83328183450641 2 15.051998729366858 20.39534046285631 40.19824664028066 24.354414167496177 3 19.53391308973264 24.722179830024526 29.64888495151564 26.095022128727198 4 12.693681086727244 16.644463192871147 37.74734168183948 32.914514038562125 5 13.816128302733027 37.4018025071916 34.04365571821239 14.738413471862986 6 31.37856123224313 36.28411499829443 17.750963719305645 14.586360050156797 7 28.19649781659942 32.78886107769333 20.992891357053306 18.021749748653946 8 26.100243201737094 34.275134356370515 20.04132434256512 19.583298099327276 9 26.146822412557665 17.123537189642818 20.743000906624058 35.98663949117547 10-11 24.68480670604755 26.618633139807624 27.541810644241377 21.15474950990345 12-13 26.063413446914 24.09533948393065 27.80283125096296 22.038415818192384 14-15 22.795648319727075 25.25426026280977 26.59768366865738 25.352407748805778 16-17 22.088896469794868 28.781954825296808 26.750076693322843 22.37907201158548 18-19 21.961616012550817 27.557071978764448 28.916120888374312 21.565191120310427 20-21 22.917226715808507 26.344078360967703 28.876877846751558 21.86181707647224 22-23 22.513032225051955 26.385747528682295 28.914219255490508 22.187000990775246 24-25 22.630950159084602 26.506570520963884 28.80305913540103 22.059420184550486 26-27 22.36435531288776 26.606219211773922 28.961355765436398 22.06806970990192 28-29 21.973469219428043 26.989582949098285 28.95718831287976 22.079759518593917 30-31 22.299070716407847 26.47752292343659 28.849942305592002 22.37346405456356 32-33 22.072926513166266 26.677321199174653 28.91801782009341 22.33173446756567 34-35 22.61659963191559 26.56923361331131 28.688868643753928 22.12529811101917 36-37 22.478741965665193 26.336837176776655 28.837435097326903 22.34698576023125 38-39 22.182165317357846 26.788081509047863 28.835336521529502 22.194416652064785 40-41 22.4733384477583 26.631251965006186 28.736662099989648 22.158747487245865 42-43 22.169748447764437 26.753034422254295 28.350354550200745 22.726862579780523 44-45 22.423586944512948 26.635156922451586 28.312013262773284 22.629242870262182 46-47 22.74895242604944 26.970473267457418 28.012721710834615 22.267852595658532 48-49 22.731076363416506 27.018698767370086 27.699107186524085 22.55111768268932 50-51 22.219655092798828 27.226232658623147 28.010761476875185 22.54335077170284 52-53 22.791364829633103 27.38723279795986 27.309586472383092 22.511815900023947 54-55 22.44088611980626 27.036616077481273 27.591347095140765 22.931150707571696 56-57 22.64404914592315 27.259104774051927 27.43351793999041 22.66332814003451 58-59 23.046380573979388 26.953556107830877 27.412866256153734 22.587197062035997 60-61 22.732545292109013 26.93679840998785 28.02817472151677 22.30248157638637 62-63 22.452372400362634 27.129863577857062 27.955133722320884 22.46263029945942 64-65 22.341818310736553 27.38630493246289 27.66455998318536 22.6073167736152 66-67 22.4917052021154 27.36240862028925 27.21866747845585 22.9272186991395 68-69 22.239189077531055 27.22224737263488 27.74441214990758 22.79415139992649 70-71 22.602502251418617 27.07421286591388 27.6051852809522 22.718099601715302 72-73 22.568563045658482 26.87708733605521 27.81838827354265 22.735961344743654 74-75 22.243435092336387 27.669945394970576 27.384837668529034 22.701781844164003 76-77 22.466303907417256 27.16049089619894 27.40013041606523 22.97307478031857 78-79 22.43219232469166 27.259401005289867 27.480376711417982 22.828029958600496 80-81 22.564778980145032 27.423374608225636 27.33616855868975 22.67567785293958 82-83 22.486584580344587 27.190921379205978 27.659232273433787 22.663261767015648 84-85 22.414092249376825 27.384715191185965 27.294527606709313 22.9066649527279 86-87 22.410664050985517 27.274760819367604 27.50087307223592 22.81370205741095 88-89 22.439596682045394 27.229060732047806 27.16500908282472 23.16633350308208 90-91 22.50404076702544 27.06598362796765 27.419671451209222 23.010304153797687 92-93 22.72955400899208 27.081219069741962 27.367212636153297 22.82201428511266 94-95 22.518036472744402 26.92407383217154 27.408281775107174 23.149607919976884 96-97 22.15001879597453 26.997321692391445 27.725693416278425 23.126966095355606 98-99 22.262494212507384 27.145197596838234 27.25360566927966 23.338702521374717 100-101 22.253484630436414 26.945480394489213 27.38759075276127 23.4134442223131 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 576.0 1 449.0 2 380.0 3 451.0 4 432.5 5 464.5 6 702.0 7 1362.5 8 2312.5 9 2806.5 10 2636.0 11 2443.5 12 2616.0 13 2883.5 14 3107.0 15 3380.5 16 3613.0 17 3736.0 18 3926.5 19 4213.0 20 4644.5 21 5386.0 22 6477.5 23 7947.0 24 9866.0 25 12492.0 26 16053.0 27 20353.5 28 24192.0 29 28603.5 30 35097.5 31 42054.5 32 48161.5 33 54225.5 34 61893.0 35 67953.0 36 74115.5 37 82457.5 38 87842.0 39 89739.5 40 91974.5 41 96365.0 42 99702.0 43 103532.0 44 108620.0 45 112434.0 46 114687.0 47 123197.5 48 141359.0 49 141533.5 50 125108.5 51 115768.0 52 110376.0 53 106811.0 54 103037.5 55 95331.0 56 86650.5 57 78623.0 58 72598.5 59 67045.0 60 58645.0 61 47446.0 62 36227.5 63 27488.5 64 20957.5 65 16172.5 66 11610.0 67 8596.0 68 6049.0 69 3664.5 70 2437.5 71 1597.0 72 997.5 73 665.0 74 468.0 75 323.0 76 217.5 77 126.5 78 68.5 79 42.0 80 26.5 81 23.0 82 22.0 83 18.0 84 13.5 85 10.5 86 11.5 87 10.5 88 7.5 89 8.0 90 10.0 91 8.0 92 4.0 93 2.5 94 1.0 95 0.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05828210182598268 2 0.09520675591443098 3 0.06809070745359105 4 0.12899898433470758 5 0.10542669016513262 6 0.10096535663773655 7 0.11036263364225167 8 0.0958395691807283 9 0.11782983018455999 10-11 0.10944505440612057 12-13 0.09209015057791671 14-15 0.0948270679546526 16-17 0.10870149881822123 18-19 0.08754971539223348 20-21 0.0722989156744682 22-23 0.07457704343313853 24-25 0.06171511379564561 26-27 0.054216276590022434 28-29 0.06829637176513768 30-31 0.0507832646203595 32-33 0.05011881069074732 34-35 0.045309429866887733 36-37 0.051431898218314245 38-39 0.05222291480118589 40-41 0.06654031495116264 42-43 0.06268015402674902 44-45 0.0497707633942838 46-47 0.03690883375679088 48-49 0.045293609535230296 50-51 0.02804944802862847 52-53 0.04092719799777883 54-55 0.04687564270097358 56-57 0.050988928931906125 58-59 0.05821882049935295 60-61 0.056067255393942084 62-63 0.06165183246901588 64-65 0.042335207515290345 66-67 0.06437292951409433 68-69 0.05432701891162447 70-71 0.025802960933273007 72-73 0.020914478451126248 74-75 0.03108695170685558 76-77 0.04334770874136606 78-79 0.06321804530310172 80-81 0.041306885957557216 82-83 0.060259643283161794 84-85 0.06559109505171666 86-87 0.06654031495116264 88-89 0.09118839167344304 90-91 0.06502156311204908 92-93 0.07520985669943585 94-95 0.08392685944268136 96-97 0.09172628294979576 98-99 0.12335112593300405 100-101 0.11703881360168834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3160490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.74582083189365 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92425477344786 36.774227330911955 2 13.818480551711005 13.748233153888451 3 4.994734314007894 7.454014748626438 4 2.335549869512517 4.6473538141088895 5 1.279692705392098 3.1829682021158283 6 0.7830278251210023 2.3371417136914046 7 0.5390213605057625 1.8769842016998255 8 0.36109635241992827 1.4370427560425663 9 0.28878021929777326 1.2929048144083788 >10 1.4823108644157372 13.358144538687563 >50 0.11963040104653905 4.117607347793983 >100 0.06806522890703928 6.096336772668407 >500 0.003697868863059866 1.2033097622632276 >1k 0.001338883553319757 1.2526283354012628 >5k 2.550254387275727E-4 0.88465785945936 >10k+ 6.375635968189317E-5 0.3364446482323483 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10608 0.3356441564440957 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9476 0.2998269255716677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7621 0.24113349512259177 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5730 0.18130100079418066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5066 0.1602916003531098 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4614 0.1459900205347905 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3520 0.11137513486832737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.164066331486573E-5 6 3.164066331486573E-5 0.0 0.0 0.0 6.328132662973146E-5 7 3.164066331486573E-5 0.0 0.0 0.0 6.328132662973146E-5 8 3.164066331486573E-5 0.0 0.0 0.0 6.328132662973146E-5 9 3.164066331486573E-5 0.0 0.0 0.0 6.328132662973146E-5 10-11 3.164066331486573E-5 0.0 0.0 0.0 6.328132662973146E-5 12-13 6.328132662973146E-5 0.0 0.0 0.0 2.5312530651892584E-4 14-15 6.328132662973146E-5 0.0 0.0 0.0 2.689456381763587E-4 16-17 6.328132662973146E-5 0.0 0.0 0.0 3.1640663314865734E-4 18-19 1.2656265325946295E-4 0.0 0.0 0.0 3.4804729646352304E-4 20-21 1.5820331657432867E-4 0.0 0.0 4.74609949722986E-5 3.4804729646352304E-4 22-23 1.5820331657432867E-4 0.0 0.0 1.7402364823176155E-4 3.4804729646352304E-4 24-25 1.898439798891944E-4 0.0 0.0 2.5312530651892584E-4 3.4804729646352304E-4 26-27 1.898439798891944E-4 0.0 0.0 5.537116080101503E-4 3.4804729646352304E-4 28-29 2.37304974861493E-4 0.0 0.0 0.0013130875275669278 3.4804729646352304E-4 30-31 2.847659698337916E-4 0.0 0.0 0.002230666763698034 3.4804729646352304E-4 32-33 3.005863014912245E-4 0.0 0.0 0.0036861372761818577 3.4804729646352304E-4 34-35 3.480472964635231E-4 0.0 0.0 0.005189068783637981 3.4804729646352304E-4 36-37 3.796879597783888E-4 0.0 0.0 0.00756211853225291 3.4804729646352304E-4 38-39 3.796879597783888E-4 0.0 0.0 0.01120079481346247 3.4804729646352304E-4 40-41 3.796879597783888E-4 0.0 0.0 0.015282440381080148 3.4804729646352304E-4 42-43 3.955082914358217E-4 0.0 0.0 0.019490648601957292 3.4804729646352304E-4 44-45 4.4296928640812024E-4 0.0 0.0 0.021484010390793833 3.638676281209559E-4 46-47 4.74609949722986E-4 0.0 0.0 0.024363310752446615 3.955082914358217E-4 48-49 5.062506130378517E-4 0.0 0.0 0.02809690902360077 5.378912763527175E-4 50-51 5.062506130378517E-4 0.0 0.0 0.03323851681226646 5.378912763527175E-4 52-53 5.062506130378517E-4 0.0 0.0 0.04334770874136605 6.802742612696132E-4 54-55 5.062506130378517E-4 0.0 0.0 0.05124205423842505 6.960945929270461E-4 56-57 5.695319396675832E-4 0.0 0.0 0.07122313312176276 7.277352562419118E-4 58-59 5.695319396675832E-4 0.0 0.0 0.10501536154203936 7.593759195567776E-4 60-61 5.695319396675832E-4 0.0 0.0 0.134093131128401 7.910165828716433E-4 62-63 5.695319396675832E-4 0.0 0.0 0.1501349474290379 8.226572461865091E-4 64-65 5.695319396675832E-4 0.0 0.0 0.1810478754876617 8.226572461865091E-4 66-67 6.960945929270461E-4 0.0 0.0 0.24008935323320119 8.38477577843942E-4 68-69 6.960945929270461E-4 1.5820331657432865E-5 0.0 0.32569316783157043 8.542979095013748E-4 70-71 7.593759195567776E-4 3.164066331486573E-5 0.0 0.39887802207885487 8.542979095013748E-4 72-73 7.593759195567776E-4 3.164066331486573E-5 0.0 0.4828207018531937 9.333995677885391E-4 74-75 7.593759195567776E-4 3.164066331486573E-5 0.0 0.5831848858879478 0.0010125012260757034 76-77 7.593759195567776E-4 3.164066331486573E-5 0.0 0.6484279336432008 0.0010125012260757034 78-79 7.593759195567776E-4 3.164066331486573E-5 0.0 0.7336204196184769 0.0010441418893905692 80-81 8.542979095013748E-4 3.164066331486573E-5 0.0 0.8465301266575752 0.0010916028843628678 82-83 8.542979095013748E-4 3.164066331486573E-5 0.0 0.94966286873238 0.0011074232160203006 84-85 8.701182411588076E-4 3.164066331486573E-5 0.0 1.1020443032567735 0.0011390638793351664 86-87 8.859385728162405E-4 3.164066331486573E-5 0.0 1.3236713294457505 0.001186524874307465 88-89 8.859385728162405E-4 3.164066331486573E-5 0.0 1.59359782818487 0.0012339858692797636 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13470 0.0 24.555231 1 TACATGG 13665 0.0 23.475998 2 GTATCAA 23680 0.0 22.216125 1 ACATGGG 13900 0.0 21.812641 3 GAGTACT 9485 0.0 21.137836 12-13 AGAGTAC 16675 0.0 20.044762 10-11 CATGGGG 6890 0.0 19.79575 4 GTACTTT 10080 0.0 19.77197 14-15 ATCAACG 26695 0.0 19.52896 3 TCAACGC 26665 0.0 19.515596 4 TATCAAC 26980 0.0 19.394354 2 CAACGCA 26825 0.0 19.380861 5 AGTACTT 10165 0.0 19.092821 12-13 AACGCAG 27305 0.0 19.09177 6 ACGCAGA 29735 0.0 17.195675 7 CGCAGAG 29760 0.0 17.03752 8 CATGGGA 8080 0.0 16.586203 4 GGTATCA 18245 0.0 16.383595 1 GTATAGG 900 0.0 16.368979 1 ACTTTTT 12630 0.0 16.306374 16-17 >>END_MODULE