FastQCFastQC Report
Thu 26 May 2016
SRR937107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160490
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119580.3783590519191644No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107950.3415609604839756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95310.3015671620539853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62030.19626703454211214No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53530.16937247072447628No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42940.13586500827403347No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32200.10188293587386765No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31660.10017434005486492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135850.022.8008751
TACATGG136350.022.0165822
ACATGGG135950.021.06783
GAGTACT116750.020.44292812-13
GTATCAA257050.020.440951
AGAGTAC189600.020.01593210-11
AGTACTT121850.019.76270312-13
CATGGGG65100.019.4800824
GTACTTT125150.019.0337614-15
GGTATCA203750.018.6299931
ATCAACG294500.017.7252963
TCAACGC294550.017.7214474
CAACGCA295550.017.6936265
TATCAAC298950.017.5570562
GTACCGT2453.7680802E-617.4479266
AACGCAG301100.017.3835376
ACTTTTT146000.016.4298416-17
TACTTTT145550.016.41497814-15
GAGTACA114200.016.0993541
AGTACAT104950.016.0669672
Produced by FastQC (version 0.11.5)