##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937107_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3160490 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01991969599651 31.0 31.0 34.0 30.0 34.0 2 32.01475752177669 33.0 31.0 34.0 30.0 34.0 3 32.201245376508076 33.0 31.0 34.0 30.0 34.0 4 35.950521596334745 37.0 35.0 37.0 35.0 37.0 5 35.86563191150739 37.0 35.0 37.0 35.0 37.0 6 35.900439805220074 37.0 35.0 37.0 35.0 37.0 7 35.83451490117039 37.0 35.0 37.0 35.0 37.0 8 35.81349885619002 37.0 35.0 37.0 33.0 37.0 9 37.47491116883774 39.0 37.0 39.0 35.0 39.0 10-11 37.39706517027423 39.0 37.0 39.0 34.0 39.0 12-13 37.303194124961635 39.0 37.0 39.0 34.0 39.0 14-15 38.48533914044974 40.0 38.0 41.0 34.0 41.0 16-17 38.40450958553895 40.0 38.0 41.0 33.5 41.0 18-19 38.45720679388323 40.0 38.0 41.0 33.5 41.0 20-21 38.53472151470183 40.0 38.0 41.0 34.0 41.0 22-23 38.46367556929464 40.0 38.0 41.0 34.0 41.0 24-25 38.36184420770197 40.0 38.0 41.0 34.0 41.0 26-27 38.20127416951169 40.0 38.0 41.0 33.5 41.0 28-29 38.031258760508656 40.0 37.0 41.0 33.0 41.0 30-31 37.910714319614996 40.0 37.0 41.0 33.0 41.0 32-33 37.90173232631649 40.0 37.0 41.0 33.0 41.0 34-35 37.821409180222055 40.0 37.0 41.0 33.0 41.0 36-37 37.66383535464438 40.0 37.0 41.0 33.0 41.0 38-39 37.517213153656556 40.0 37.0 41.0 32.5 41.0 40-41 37.35626421852308 40.0 36.5 41.0 32.0 41.0 42-43 37.14186834952808 40.0 36.0 41.0 31.0 41.0 44-45 36.830901062809886 39.0 36.0 41.0 31.0 41.0 46-47 36.57981879392119 39.0 36.0 41.0 30.0 41.0 48-49 36.27078522634149 39.0 35.0 41.0 30.0 41.0 50-51 36.00800508781866 39.0 35.0 40.0 29.0 41.0 52-53 35.757120414872375 39.0 35.0 40.0 28.5 41.0 54-55 35.51820144977519 38.0 35.0 40.0 28.0 41.0 56-57 35.238008188603665 38.0 34.0 40.0 27.5 41.0 58-59 34.903874873832855 38.0 34.0 40.0 26.5 41.0 60-61 34.55315916202867 37.5 34.0 40.0 26.0 41.0 62-63 34.19083828773387 37.0 33.0 40.0 26.0 41.0 64-65 33.79104632509516 36.5 33.0 39.0 25.5 40.5 66-67 33.39584684653329 36.0 32.5 39.0 24.5 40.0 68-69 32.960280051511 35.5 32.0 38.5 23.5 40.0 70-71 32.47347151865692 35.0 31.5 38.0 22.5 40.0 72-73 32.11995307689631 35.0 31.0 37.0 22.0 39.5 74-75 31.68451015507089 35.0 31.0 37.0 20.5 39.0 76-77 30.274061458824423 33.5 29.5 35.5 20.0 37.5 78-79 31.001708121209052 34.0 31.0 36.0 20.0 38.0 80-81 30.83049432208297 34.0 31.0 35.5 19.5 37.0 82-83 30.50560957319909 34.0 30.5 35.0 18.0 37.0 84-85 30.121058601672523 34.0 30.0 35.0 16.0 36.5 86-87 29.77547611288123 34.0 30.0 35.0 11.0 36.0 88-89 29.41183028581011 34.0 29.0 35.0 7.0 36.0 90-91 28.97569221861167 34.0 29.0 35.0 2.0 35.0 92-93 28.510748491531377 34.0 29.0 35.0 2.0 35.0 94-95 28.022577511714957 33.0 28.0 35.0 2.0 35.0 96-97 27.37700752098567 33.0 27.0 35.0 2.0 35.0 98-99 26.634645418906562 33.0 25.5 34.5 2.0 35.0 100-101 23.64801518119026 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35911260819946733 1101 2 0.276059154560393 1101 3 0.2688400950404741 1101 4 0.2635842585055883 1101 5 0.3430953678593909 1101 6 0.2742649146080893 1101 7 0.3415756851634342 1101 8 0.3957436498684075 1101 9 0.48180256388945253 1101 10-11 0.4680192604498288 1101 12-13 0.5146264397889198 1101 14-15 0.5966812793051446 1101 16-17 0.5860117141304713 1101 18-19 0.522249567209748 1101 20-21 0.5766802845524808 1101 22-23 0.47391447410901577 1101 24-25 0.5117811296705597 1101 26-27 0.4840233636046065 1101 28-29 0.5495652183076345 1101 30-31 0.5391531983292239 1101 32-33 0.6528300937015104 1101 34-35 0.550115265939823 1101 36-37 0.6674540483090041 1101 38-39 0.6295103026956994 1101 40-41 0.5655199969871205 1101 42-43 0.6775828371702275 1101 44-45 0.8299569135586893 1101 46-47 0.7644427317416742 1101 48-49 0.6547079702829848 1101 50-51 0.7630781085299105 1101 52-53 0.5907875861117731 1101 54-55 0.6986802556114782 1101 56-57 0.7135843297762037 1101 58-59 0.7082306150254709 1101 60-61 0.752976176384756 1101 62-63 0.8468837204217934 1101 64-65 0.8338346093562947 1101 66-67 0.9290769875325822 1101 68-69 0.8114067896552442 1101 70-71 0.7714451272834353 1101 72-73 0.8979564290032442 1101 74-75 0.7709877995180072 1101 76-77 0.565386399720456 1101 78-79 0.6663104117636323 1101 80-81 0.8827567544377786 1101 82-83 1.080744219730711 1101 84-85 0.9743094793150142 1101 86-87 0.7946574038422725 1101 88-89 0.8992429222724709 1101 90-91 1.0181083704674236 1101 92-93 1.0702163025754352 1101 94-95 1.1602173168886054 1101 96-97 1.2342866604222031 1101 98-99 1.1511523993443582 1101 100-101 1.3796159294585664 1102 1 0.23615218859253417 1102 2 0.11416502526584793 1102 3 0.15631929374289655 1102 4 0.19225337760295247 1102 5 0.2140851936551087 1102 6 0.21944427972089642 1102 7 0.25089830491092613 1102 8 0.2866270802585049 1102 9 0.3921577322818237 1102 10-11 0.36398816526271105 1102 12-13 0.3428332865515813 1102 14-15 0.468493007694299 1102 16-17 0.4109042367701363 1102 18-19 0.43340394587092845 1102 20-21 0.44574662465393544 1102 22-23 0.3953056763809357 1102 24-25 0.37233918850974845 1102 26-27 0.4271127368943013 1102 28-29 0.3968336177614873 1102 30-31 0.36859165425536133 1102 32-33 0.4162765886469586 1102 34-35 0.36929880184444386 1102 36-37 0.4564405152576825 1102 38-39 0.5490415960816009 1102 40-41 0.4129878399524003 1102 42-43 0.3510620205529378 1102 44-45 0.5144223417542335 1102 46-47 0.5467131323330179 1102 48-49 0.5344035101400948 1102 50-51 0.5176595644005175 1102 52-53 0.6114234307928612 1102 54-55 0.4825523321794023 1102 56-57 0.6275807066641121 1102 58-59 0.5938028924731285 1102 60-61 0.514091777022827 1102 62-63 0.7066918793178516 1102 64-65 0.6036699990419052 1102 66-67 0.6068480842133752 1102 68-69 0.4379660970510244 1102 70-71 0.34981800143390274 1102 72-73 0.6365627755772749 1102 74-75 0.435209280436343 1102 76-77 0.5405041221387528 1102 78-79 0.5605182198382721 1102 80-81 0.5976163971437778 1102 82-83 0.6399936878494294 1102 84-85 0.5909808695598038 1102 86-87 0.44876519334176024 1102 88-89 0.6134214122466801 1102 90-91 0.7997007780177441 1102 92-93 0.7239663767802931 1102 94-95 0.7419991143888076 1102 96-97 0.881573730776072 1102 98-99 1.0915353538658756 1102 100-101 1.2576902607052318 1103 1 0.12423804667000127 1103 2 -0.08748266168199237 1103 3 0.062218420749722725 1103 4 0.15236780464825728 1103 5 0.15493766206029846 1103 6 0.1922734891429556 1103 7 0.2646976513016668 1103 8 0.23741033207541307 1103 9 0.18234392697569035 1103 10-11 0.22392977796160807 1103 12-13 0.3215386561700555 1103 14-15 0.2831193227894886 1103 16-17 0.39028098585446 1103 18-19 0.41466955158239927 1103 20-21 0.40214411691081153 1103 22-23 0.42023038921404066 1103 24-25 0.4774065317767153 1103 26-27 0.4366168899986178 1103 28-29 0.488329556597094 1103 30-31 0.45285259356157326 1103 32-33 0.5547808097068696 1103 34-35 0.5307928182998722 1103 36-37 0.4953993301702653 1103 38-39 0.5728611220132009 1103 40-41 0.4847817440812321 1103 42-43 0.550791052163909 1103 44-45 0.6727009139018136 1103 46-47 0.658837954767229 1103 48-49 0.5759183820583331 1103 50-51 0.39721717711686466 1103 52-53 0.5248497300438615 1103 54-55 0.4745517420195924 1103 56-57 0.47550662561816637 1103 58-59 0.5883209292323812 1103 60-61 0.5088099557256811 1103 62-63 0.5346972699771229 1103 64-65 0.5783031205984841 1103 66-67 0.6160967678917117 1103 68-69 0.618724888488039 1103 70-71 0.4331755310772607 1103 72-73 0.6804906161846986 1103 74-75 0.7018527683099087 1103 76-77 0.7254884251233129 1103 78-79 0.6278322331902757 1103 80-81 0.6644749808256414 1103 82-83 0.7955434976646174 1103 84-85 0.8897188396935043 1103 86-87 0.8142333764962579 1103 88-89 0.912089246392874 1103 90-91 1.0873577163747505 1103 92-93 1.0449230098458884 1103 94-95 1.063781026353972 1103 96-97 1.3163787872537114 1103 98-99 1.2733938812209082 1103 100-101 1.3936248881504945 1104 1 0.20269001448738067 1104 2 0.07780693898660473 1104 3 0.08937964637392781 1104 4 0.06703103893713092 1104 5 0.07030734326703936 1104 6 0.0958880922858043 1104 7 0.15849666848377808 1104 8 0.18146995243512976 1104 9 0.11650477693969918 1104 10-11 0.12074365447334401 1104 12-13 0.16649226976220888 1104 14-15 0.1995261489503335 1104 16-17 0.21998284858275952 1104 18-19 0.2646949325901673 1104 20-21 0.13976418007422353 1104 22-23 0.18471503414775015 1104 24-25 0.20347335885276863 1104 26-27 0.01117869958110873 1104 28-29 0.1416049481581112 1104 30-31 0.12650222994449223 1104 32-33 0.12651434850043586 1104 34-35 0.10049977851359415 1104 36-37 0.0528192044039173 1104 38-39 0.11017111475185715 1104 40-41 0.15329008575961467 1104 42-43 0.1870215273226279 1104 44-45 0.2536164002895447 1104 46-47 0.34588471931838427 1104 48-49 0.31865650504146004 1104 50-51 0.27426570468141875 1104 52-53 0.24789256346534927 1104 54-55 0.19886176349146467 1104 56-57 0.2462658423294073 1104 58-59 0.16457853067571193 1104 60-61 0.2972387702463877 1104 62-63 0.47408064805977546 1104 64-65 0.2930955302050151 1104 66-67 0.4036833621790521 1104 68-69 0.38988539471764483 1104 70-71 0.29131201527381023 1104 72-73 0.2481081217371326 1104 74-75 0.2962044032831308 1104 76-77 0.21696024688371907 1104 78-79 0.34368342634595095 1104 80-81 0.5233844240149281 1104 82-83 0.47751698719313396 1104 84-85 0.5148050836839175 1104 86-87 0.40598155149360693 1104 88-89 0.4765214443384842 1104 90-91 0.4922937814210755 1104 92-93 0.6118224510380408 1104 94-95 0.42653610902936734 1104 96-97 0.7329085277289167 1104 98-99 0.7183197548437086 1104 100-101 0.6451685003840346 1105 1 0.12490020405470403 1105 2 0.006174030086782523 1105 3 0.03858049444147582 1105 4 0.07666634521454796 1105 5 0.033896280431378045 1105 6 0.023739783702254158 1105 7 0.1376453578600092 1105 8 0.10035328961809853 1105 9 0.13086080019881763 1105 10-11 0.1795167720739741 1105 12-13 0.23310835235268712 1105 14-15 0.3153669856027861 1105 16-17 0.2986137137851017 1105 18-19 0.22893748275755854 1105 20-21 0.22187740393879096 1105 22-23 0.23017561515292329 1105 24-25 0.16830417375781082 1105 26-27 0.1817880738940616 1105 28-29 0.2083124053510872 1105 30-31 0.2576638457637799 1105 32-33 0.17748534377711422 1105 34-35 0.23113517568766184 1105 36-37 0.12892629700222358 1105 38-39 0.297576861610402 1105 40-41 0.020896889765396054 1105 42-43 0.07112863799599722 1105 44-45 0.2540020158849856 1105 46-47 0.2853322518738963 1105 48-49 0.31730526454542485 1105 50-51 0.31956734688680655 1105 52-53 0.27958920951775923 1105 54-55 0.3247167438722087 1105 56-57 0.3490047862632011 1105 58-59 0.31580593099484133 1105 60-61 0.39459664950052087 1105 62-63 0.27949697647095917 1105 64-65 0.30064907711247457 1105 66-67 0.3766434317819076 1105 68-69 0.3614897050614232 1105 70-71 0.07263581992629753 1105 72-73 0.24570670333217492 1105 74-75 0.2798212368572628 1105 76-77 0.09706894746648587 1105 78-79 0.2096386224628759 1105 80-81 0.45750264041744515 1105 82-83 0.524580338686679 1105 84-85 0.5404508018865535 1105 86-87 0.5541811633318616 1105 88-89 0.5489376199807161 1105 90-91 0.5932877007302224 1105 92-93 0.6493287925136606 1105 94-95 0.6474680795732901 1105 96-97 0.8754609892642513 1105 98-99 0.7263807935995779 1105 100-101 0.9695418218156391 1106 1 0.319078720164633 1106 2 0.18965700906626637 1106 3 0.2128260909783961 1106 4 0.21491096180778868 1106 5 0.22145752098393956 1106 6 0.28180556341808227 1106 7 0.290069850647626 1106 8 0.36906727856082 1106 9 0.4237902674905385 1106 10-11 0.3942873839687593 1106 12-13 0.4273638830070823 1106 14-15 0.6207021017785692 1106 16-17 0.6256573995266876 1106 18-19 0.5615415806711184 1106 20-21 0.5346308133261957 1106 22-23 0.5783231829871411 1106 24-25 0.5569880435641466 1106 26-27 0.561422023384857 1106 28-29 0.6105536329249901 1106 30-31 0.5200562399894721 1106 32-33 0.5516385038915814 1106 34-35 0.5199066637651555 1106 36-37 0.5246024140427181 1106 38-39 0.5631108706926184 1106 40-41 0.5217833157906924 1106 42-43 0.5832315400500931 1106 44-45 0.5905485566183231 1106 46-47 0.5098000097633104 1106 48-49 0.45681730152985267 1106 50-51 0.36459026772410397 1106 52-53 0.4836341708364458 1106 54-55 0.6966697729671125 1106 56-57 0.5797221037138343 1106 58-59 0.48048455698328496 1106 60-61 0.4623073722488229 1106 62-63 0.5347356964247396 1106 64-65 0.6439584812427839 1106 66-67 0.6956164295531337 1106 68-69 0.7855205951020139 1106 70-71 0.7388150913857814 1106 72-73 0.8346377784447867 1106 74-75 0.7762957348471566 1106 76-77 0.6710662669430185 1106 78-79 0.8205940552272324 1106 80-81 0.7789021179894746 1106 82-83 0.8160444430326628 1106 84-85 0.7814918146923091 1106 86-87 0.7558643769022027 1106 88-89 0.9269369547343942 1106 90-91 1.21237632464819 1106 92-93 1.2298412389461113 1106 94-95 1.0151590839425388 1106 96-97 1.3481586693338805 1106 98-99 1.4040609353139253 1106 100-101 1.525712123736369 1107 1 0.20741866532851816 1107 2 0.09259598246867995 1107 3 0.0844630191300908 1107 4 0.13711856283885737 1107 5 0.17706279745458176 1107 6 0.12199122658345374 1107 7 0.15336804563023065 1107 8 0.14779920145895886 1107 9 0.17201003140231563 1107 10-11 0.10664725181953116 1107 12-13 0.17113137073373963 1107 14-15 0.1752594396756777 1107 16-17 0.19440813683934977 1107 18-19 0.19754140704051082 1107 20-21 0.11147816980027159 1107 22-23 0.20068806788847127 1107 24-25 0.17593977634627578 1107 26-27 0.22291505849907622 1107 28-29 0.17654166799076876 1107 30-31 0.23966898485563348 1107 32-33 0.24997803302591137 1107 34-35 0.25601840018705957 1107 36-37 0.28080795088327193 1107 38-39 0.2981670983227147 1107 40-41 0.2997508556065256 1107 42-43 0.29047695132951645 1107 44-45 0.40267046125864425 1107 46-47 0.4195388343829194 1107 48-49 0.4709419325885733 1107 50-51 0.4696146985377254 1107 52-53 0.4691914386354412 1107 54-55 0.3364354267442735 1107 56-57 0.4311367182054582 1107 58-59 0.4604330244082604 1107 60-61 0.5718216453872387 1107 62-63 0.3633502761902392 1107 64-65 0.3743305769432652 1107 66-67 0.4413963890481938 1107 68-69 0.48423022907388713 1107 70-71 0.4324405211642599 1107 72-73 0.4663012090093517 1107 74-75 0.48712916363932734 1107 76-77 0.4217312968468043 1107 78-79 0.5721640444123643 1107 80-81 0.5717259499864795 1107 82-83 0.5418328522970874 1107 84-85 0.5951146077172211 1107 86-87 0.5441554338433541 1107 88-89 0.7229927095345481 1107 90-91 0.8666208520072587 1107 92-93 0.971062587759544 1107 94-95 0.9120167594694948 1107 96-97 1.1303525185932628 1107 98-99 1.0446282533613065 1107 100-101 0.9693930716150803 1108 1 -0.06594369316638904 1108 2 -0.1888302412845171 1108 3 -0.10569511403014076 1108 4 0.024272477893468647 1108 5 -0.020431096219844846 1108 6 -0.05166881064123885 1108 7 -0.031186330004480567 1108 8 -0.02813229480248225 1108 9 -0.025237034516294443 1108 10-11 -0.007194932900716822 1108 12-13 -0.018245182461477327 1108 14-15 -0.07101153016181883 1108 16-17 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-0.8963905198972206 2316 84-85 -0.8315707656054734 2316 86-87 -0.7966662565403126 2316 88-89 -0.9842321221817905 2316 90-91 -0.9138071608759901 2316 92-93 -1.2870920891962534 2316 94-95 -1.2136446894012067 2316 96-97 -0.9270049746960041 2316 98-99 -0.9536119147256308 2316 100-101 -0.9811627252174944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 23.0 5 66.0 6 155.0 7 387.0 8 885.0 9 1772.0 10 2844.0 11 4614.0 12 6861.0 13 9318.0 14 12121.0 15 15936.0 16 21153.0 17 28012.0 18 25765.0 19 17458.0 20 15670.0 21 16396.0 22 17404.0 23 19512.0 24 22731.0 25 27930.0 26 34150.0 27 42801.0 28 53893.0 29 66335.0 30 82475.0 31 103236.0 32 129013.0 33 165882.0 34 221623.0 35 307885.0 36 462136.0 37 682163.0 38 501479.0 39 40392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85637883614829 19.563679816251593 14.88569680816893 22.694244539431192 2 15.37009192495643 21.186278716265388 39.409455246860844 24.034174111917338 3 19.681781794134306 24.79640299834515 30.008448270952186 25.513366936568353 4 13.053894943872486 17.312644084035284 37.227138139056 32.40632283303623 5 13.748001868087222 37.740234624667615 33.86395363701141 14.647809870233752 6 31.39595884169493 36.78817774515263 17.294588163823217 14.521275249329216 7 27.955352436302515 33.07586238319043 20.83796605965032 18.130819120856728 8 26.210587598758423 34.61577160503593 19.71678442266864 19.456856373537015 9 26.06532531347987 17.127755506266436 20.608703080851388 36.19821609940231 10-11 24.74842868287167 26.884793491379728 27.24693721898107 21.119840606767536 12-13 26.115563337811576 24.246234574268478 27.77720121020128 21.861000877718663 14-15 22.75650294732779 25.47884979860718 26.545187613313125 25.219459640751907 16-17 21.960676983632286 28.95777237073998 26.797996513198903 22.283554132428833 18-19 21.862211239396423 27.601020727798538 29.130641134760747 21.40612689804429 20-21 22.78194037501345 26.325472563773168 29.259085817707774 21.63350124350561 22-23 22.40976873839183 26.358745004730277 29.250954757015524 21.980531499862362 24-25 22.56494415683738 26.53144300554777 29.14098005644278 21.762632781172073 26-27 22.310033636197808 26.61785903172491 29.290308025684443 21.78179930639284 28-29 21.884097431199077 27.023945827187628 29.246256294091467 21.84570044752183 30-31 22.23894865856055 26.42698530791604 29.173455957767104 22.160610075756303 32-33 22.032143310285083 26.654966933726016 29.241353583057716 22.071536172931186 34-35 22.620363749121953 26.46428322817853 29.03452705653046 21.880825966169056 36-37 22.456462266031547 26.319405814903483 29.14139266324554 22.082739255819426 38-39 22.15320865115876 26.806751910907735 29.126752265292243 21.91328717264126 40-41 22.403836524345877 26.6197163266659 29.013778649619244 21.96266849936898 42-43 22.147669800431334 26.794273285542157 28.603576238064864 22.454480675961648 44-45 22.455392018356722 26.665500176081014 28.50557051989304 22.373537285669222 46-47 22.765129402607513 27.03317687000571 28.128752390851403 22.072941336535372 48-49 22.76642307012641 27.147079143378445 27.796789448962826 22.289708337532318 50-51 22.296950532291554 27.312887954574077 28.055511082789163 22.334650430345206 52-53 22.879916265168315 27.486724213269426 27.369035388281365 22.264324133280894 54-55 22.510139545715443 27.15564621924201 27.637500087014356 22.696714148028192 56-57 22.762258837674718 27.363339371455243 27.46876656198831 22.405635228881735 58-59 23.168717482863986 27.06579033548714 27.413098581878714 22.35239359977016 60-61 22.809957102729587 27.1209785292161 28.039896122634143 22.029168245420163 62-63 22.55102847976105 27.227186290733403 27.971517074883955 22.250268154621594 64-65 22.42186285045853 27.466706449596572 27.704518088919595 22.406912611025305 66-67 22.578301199055336 27.458528556349528 27.31771169515594 22.6454585494392 68-69 22.364443488193288 27.29037269537319 27.724925565339554 22.620258251093976 70-71 22.68383071255734 27.121507554621804 27.6246111155496 22.570050617271256 72-73 22.700894481551913 26.932437691623768 27.83204503099203 22.53462279583229 74-75 22.383140266485093 27.753721985636975 27.30677995758235 22.55635779029558 76-77 22.595535251961774 27.314944820698315 27.35228104558516 22.737238881754752 78-79 22.548163912262158 27.375099411213505 27.440833024440725 22.635903652083613 80-81 22.71510554368354 27.513533807345535 27.338789204216717 22.432571444754203 82-83 22.60276692442082 27.344384429955255 27.542853599047344 22.509995046576577 84-85 22.51819872635811 27.48874662778534 27.295937901256366 22.697116744600184 86-87 22.50709158707583 27.39524780607733 27.40119635429933 22.696464252547514 88-89 22.576732908616613 27.35054823515378 27.06571623524997 23.007002620979637 90-91 22.604642612435725 27.156398696591832 27.306518132431318 22.932440558541124 92-93 22.78385850284173 27.172975688086094 27.250511194871045 22.792654614201133 94-95 22.599471409573415 27.065073322699927 27.234905066665487 23.100550201061168 96-97 22.223283958383035 27.19004177263813 27.58416386062456 23.002510408354272 98-99 22.268687800673582 27.335919711530547 27.00948992597149 23.385902561824373 100-101 22.02544346018149 27.37555326228773 27.165583727804048 23.433419549726725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 704.0 1 507.0 2 345.0 3 415.0 4 390.5 5 366.0 6 641.0 7 1426.5 8 2399.5 9 2932.0 10 2821.0 11 2621.5 12 2772.0 13 3044.0 14 3413.0 15 3751.0 16 3953.0 17 4244.5 18 4543.0 19 4834.0 20 5273.0 21 5982.5 22 7132.5 23 8655.0 24 10507.5 25 13027.0 26 16633.0 27 20862.5 28 24618.5 29 29515.5 30 36126.0 31 42704.5 32 48761.5 33 54662.5 34 61832.5 35 67373.0 36 73745.5 37 82037.5 38 87234.5 39 89278.0 40 91302.0 41 96176.5 42 99811.0 43 103310.0 44 108850.0 45 112557.0 46 114024.5 47 123132.5 48 143157.0 49 142818.0 50 124577.0 51 114951.0 52 109726.0 53 106689.5 54 102969.5 55 94846.5 56 85846.0 57 77772.0 58 71779.5 59 66636.0 60 58029.5 61 46593.5 62 35816.5 63 27116.5 64 20314.0 65 15289.5 66 10944.5 67 8256.5 68 5878.0 69 3491.5 70 2196.0 71 1379.0 72 890.0 73 601.0 74 400.0 75 256.0 76 170.5 77 88.5 78 37.0 79 23.0 80 15.0 81 8.5 82 8.0 83 6.0 84 3.0 85 2.5 86 3.5 87 3.0 88 1.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016358222933785586 2 0.002721097045078453 3 0.0025312530651892587 4 2.5312530651892584E-4 5 0.0012023452059648978 6 3.1640663314865734E-4 7 1.2656265325946292E-4 8 0.0 9 0.0 10-11 1.423829849168958E-4 12-13 7.593759195567776E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002214846432040601 22-23 0.0 24-25 0.0038285202610987535 26-27 0.001756056813975048 28-29 0.00270527671342102 30-31 0.015472284360969342 32-33 6.011726029824489E-4 34-35 0.002214846432040601 36-37 0.0040500049043028135 38-39 0.0025628937285041243 40-41 0.0040974658992751125 42-43 0.0023414090853000643 44-45 4.1132862309325454E-4 46-47 1.5820331657432867E-4 48-49 1.423829849168958E-4 50-51 1.2656265325946292E-4 52-53 0.0011707045426500322 54-55 0.0029109410249676475 56-57 4.74609949722986E-4 58-59 0.001297267195909495 60-61 0.0010283215577331364 62-63 0.0 64-65 1.7402364823176155E-4 66-67 6.328132662973146E-5 68-69 0.0 70-71 2.37304974861493E-4 72-73 0.0 74-75 4.1132862309325454E-4 76-77 6.486335979547475E-4 78-79 2.0566431154662727E-4 80-81 0.005173248451980548 82-83 0.0015662128340858538 84-85 0.0032906289847460363 86-87 0.0017402364823176151 88-89 9.650402311034048E-4 90-91 2.0566431154662727E-4 92-93 7.910165828716434E-5 94-95 2.847659698337916E-4 96-97 0.0015187518391135552 98-99 0.002721097045078453 100-101 9.966808944182706E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3160490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.033028424683316 #Duplication Level Percentage of deduplicated Percentage of total 1 71.41620758312982 33.58920521240429 2 15.066591847248468 14.172548852294783 3 5.407788618635125 7.6303402744483435 4 2.604648310698362 4.9001799213351775 5 1.46985288776748 3.4565816325235375 6 0.8688571613484334 2.4518990140014316 7 0.5992737910659883 1.9729962874562053 8 0.40621903682884763 1.5284569204654936 9 0.30128022024844886 1.275310904646613 >10 1.644222452850095 14.058031658495922 >50 0.13270102264422706 4.306557650257576 >100 0.0764279481228697 6.575823269803255 >500 0.00410995777425506 1.3261905557564817 >1k 0.001482279852810378 1.3666258996777754 >5k 2.0212907083777884E-4 0.6682289186686743 >10k+ 1.3475271389185256E-4 0.721023027764421 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11958 0.3783590519191644 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10795 0.3415609604839756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9531 0.3015671620539853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6203 0.19626703454211214 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5353 0.16937247072447628 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4294 0.13586500827403347 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3220 0.10188293587386765 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3166 0.10017434005486492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.164066331486573E-5 2 0.0 0.0 0.0 0.0 3.164066331486573E-5 3 0.0 0.0 0.0 0.0 3.164066331486573E-5 4 0.0 0.0 0.0 0.0 3.164066331486573E-5 5 0.0 0.0 0.0 0.0 3.164066331486573E-5 6 0.0 0.0 0.0 0.0 3.164066331486573E-5 7 3.164066331486573E-5 0.0 0.0 0.0 3.164066331486573E-5 8 3.164066331486573E-5 0.0 0.0 0.0 3.164066331486573E-5 9 3.164066331486573E-5 0.0 0.0 0.0 3.164066331486573E-5 10-11 3.164066331486573E-5 0.0 0.0 0.0 3.164066331486573E-5 12-13 3.164066331486573E-5 0.0 0.0 0.0 2.0566431154662724E-4 14-15 9.49219899445972E-5 0.0 0.0 0.0 2.37304974861493E-4 16-17 1.2656265325946292E-4 0.0 0.0 0.0 2.847659698337916E-4 18-19 1.423829849168958E-4 0.0 0.0 0.0 3.1640663314865734E-4 20-21 1.898439798891944E-4 0.0 0.0 6.328132662973146E-5 4.587896180655531E-4 22-23 2.37304974861493E-4 0.0 0.0 2.0566431154662724E-4 5.537116080101504E-4 24-25 3.1640663314865734E-4 0.0 0.0 2.847659698337916E-4 5.695319396675832E-4 26-27 3.1640663314865734E-4 0.0 0.0 5.853522713250161E-4 5.695319396675832E-4 28-29 3.955082914358217E-4 0.0 0.0 0.0013605485225392266 6.011726029824489E-4 30-31 4.4296928640812024E-4 0.0 0.0 0.0023097684219851983 6.011726029824489E-4 32-33 4.4296928640812024E-4 0.0 0.0 0.0037494186028115893 6.011726029824489E-4 34-35 4.587896180655531E-4 0.0 0.0 0.005268170441925145 6.644539296121804E-4 36-37 4.74609949722986E-4 0.0 0.0 0.00767286085385494 6.644539296121804E-4 38-39 5.062506130378517E-4 0.0 0.0 0.011406459125009098 6.644539296121804E-4 40-41 5.062506130378517E-4 0.0 0.0 0.015662128340858537 6.644539296121804E-4 42-43 5.220709446952846E-4 0.0 0.0 0.019854516230078247 6.644539296121804E-4 44-45 5.378912763527175E-4 0.0 0.0 0.021847878018914788 7.277352562419118E-4 46-47 6.169929346398818E-4 0.0 0.0 0.02467971738559527 7.910165828716433E-4 48-49 6.328132662973147E-4 0.0 0.0 0.0283500343301197 8.701182411588076E-4 50-51 6.328132662973147E-4 0.0 0.0 0.03350746245044281 9.808605627608378E-4 52-53 6.328132662973147E-4 0.0 0.0 0.04355337305291268 0.0010125012260757034 54-55 6.328132662973147E-4 0.0 0.0 0.051479359213286544 0.0010125012260757034 56-57 6.328132662973147E-4 0.0 0.0 0.07146043809662425 0.0010283215577331362 58-59 6.328132662973147E-4 0.0 0.0 0.1051735648586137 0.0010441418893905692 60-61 6.328132662973147E-4 0.0 0.0 0.1342513344449753 0.0010441418893905692 62-63 6.328132662973147E-4 0.0 0.0 0.15013494742903788 0.0010441418893905692 64-65 6.328132662973147E-4 0.0 0.0 0.18062072653291103 0.0010441418893905692 66-67 6.486335979547476E-4 0.0 0.0 0.23950400096187616 0.0010441418893905692 68-69 6.644539296121804E-4 0.0 0.0 0.32550332385168124 0.0010441418893905692 70-71 7.11914924584479E-4 0.0 0.0 0.39887802207885487 0.0010441418893905692 72-73 7.593759195567776E-4 0.0 0.0 0.48321621014462945 0.0010757825527054348 74-75 7.593759195567776E-4 0.0 0.0 0.5845137937471722 0.0011074232160203006 76-77 7.593759195567776E-4 0.0 0.0 0.6502789124471205 0.0011074232160203006 78-79 7.593759195567776E-4 0.0 0.0 0.735597961075656 0.0011232435476777334 80-81 7.593759195567776E-4 0.0 0.0 0.8490930203860794 0.0011390638793351664 82-83 7.593759195567776E-4 0.0 0.0 0.9521941217975693 0.001186524874307465 84-85 7.910165828716434E-4 0.0 0.0 1.1053032915782046 0.0012023452059648978 86-87 8.226572461865091E-4 0.0 0.0 1.3271992634053582 0.0012181655376223306 88-89 8.226572461865091E-4 0.0 0.0 1.5983122870187851 0.0012339858692797636 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13585 0.0 22.800875 1 TACATGG 13635 0.0 22.016582 2 ACATGGG 13595 0.0 21.0678 3 GAGTACT 11675 0.0 20.442928 12-13 GTATCAA 25705 0.0 20.44095 1 AGAGTAC 18960 0.0 20.015932 10-11 AGTACTT 12185 0.0 19.762703 12-13 CATGGGG 6510 0.0 19.480082 4 GTACTTT 12515 0.0 19.03376 14-15 GGTATCA 20375 0.0 18.629993 1 ATCAACG 29450 0.0 17.725296 3 TCAACGC 29455 0.0 17.721447 4 CAACGCA 29555 0.0 17.693626 5 TATCAAC 29895 0.0 17.557056 2 GTACCGT 245 3.7680802E-6 17.447926 6 AACGCAG 30110 0.0 17.383537 6 ACTTTTT 14600 0.0 16.42984 16-17 TACTTTT 14555 0.0 16.414978 14-15 GAGTACA 11420 0.0 16.099354 1 AGTACAT 10495 0.0 16.066967 2 >>END_MODULE