FastQCFastQC Report
Thu 26 May 2016
SRR937106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856725
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95830.33545406015629786No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87880.30762499015481015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70030.245140851849583No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54770.19172303949452607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.15944831931670006No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43780.15325241316542546No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33540.11740717079872932No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29320.10263501037026665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126350.025.9564821
TACATGG126550.025.089172
ACATGGG125850.024.3222983
GTATCAA207850.021.907231
CATGGGG61350.021.150474
GAGTACT86850.020.79136712-13
AGAGTAC150450.020.64242410-11
AGTACTT92550.019.45951712-13
CATGGGA72250.019.4068984
GTACTTT94800.019.04678514-15
ATCAACG237450.018.8960843
CAACGCA236800.018.8686585
TCAACGC236900.018.8597014
TATCAAC240750.018.7160432
AACGCAG240750.018.5771926
GGTATCA160500.017.9164661
GAGTACA99800.017.097631
ACCGTAT1952.2665923E-417.061328
CGCGGAA3500.016.96505482-83
ACGCAGA261450.016.9427557
Produced by FastQC (version 0.11.5)