##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937106_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2856725 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52448695621735 31.0 31.0 34.0 30.0 34.0 2 31.63476113381582 33.0 31.0 34.0 30.0 34.0 3 31.647381529548696 33.0 31.0 34.0 28.0 34.0 4 35.23874156595402 37.0 35.0 37.0 33.0 37.0 5 35.24266143923549 37.0 35.0 37.0 33.0 37.0 6 35.3021508895676 37.0 35.0 37.0 33.0 37.0 7 35.228501868398254 37.0 35.0 37.0 32.0 37.0 8 35.17515476638459 37.0 35.0 37.0 32.0 37.0 9 36.773356553395935 39.0 37.0 39.0 32.0 39.0 10-11 36.57529268655541 39.0 37.0 39.0 32.0 39.0 12-13 36.49844507259186 39.0 37.0 39.0 32.0 39.0 14-15 37.59796917799228 40.0 37.0 41.0 32.0 41.0 16-17 37.52601773009303 40.0 37.0 41.0 32.0 41.0 18-19 37.479113320323094 40.0 37.0 41.0 32.0 41.0 20-21 37.397332959945395 40.0 37.0 41.0 32.0 41.0 22-23 37.274829743850034 40.0 37.0 41.0 31.0 41.0 24-25 37.10406706980896 39.0 36.0 41.0 31.0 41.0 26-27 37.205410216244125 40.0 37.0 41.0 31.0 41.0 28-29 37.161136616230124 40.0 37.0 41.0 31.0 41.0 30-31 37.02860705878234 39.5 36.5 41.0 30.5 41.0 32-33 36.82997523387795 39.0 36.0 41.0 30.0 41.0 34-35 36.65034628814464 39.0 36.0 41.0 30.0 41.0 36-37 36.466338376987636 39.0 35.0 41.0 30.0 41.0 38-39 36.30452318651602 39.0 35.0 41.0 30.0 41.0 40-41 36.14388819364832 39.0 35.0 40.5 29.5 41.0 42-43 35.92502901049278 39.0 35.0 40.0 28.5 41.0 44-45 35.68529294909381 39.0 35.0 40.0 27.5 41.0 46-47 35.34066877280802 38.0 35.0 40.0 26.5 41.0 48-49 35.038490054170424 38.0 34.0 40.0 25.5 41.0 50-51 34.380247486194854 37.5 33.5 39.5 24.5 40.5 52-53 34.624818979775796 38.0 34.0 39.5 25.5 40.5 54-55 35.010031942171544 38.0 34.0 40.0 26.0 41.0 56-57 34.884105750466006 38.0 34.0 40.0 25.5 41.0 58-59 34.63129335165267 38.0 34.0 40.0 24.5 41.0 60-61 34.326612817124506 38.0 34.0 40.0 23.5 41.0 62-63 33.998287199502926 37.5 33.5 40.0 23.0 41.0 64-65 33.68004375639937 37.0 33.0 40.0 22.5 41.0 66-67 33.28340214756408 36.0 33.0 39.0 22.0 41.0 68-69 32.888421006572216 36.0 32.0 39.0 21.0 41.0 70-71 32.455890924047644 35.0 32.0 39.0 20.0 40.0 72-73 32.054902029421804 35.0 31.5 38.0 18.5 40.0 74-75 31.623748173170327 35.0 31.0 37.0 18.0 39.0 76-77 31.185551986978098 35.0 31.0 37.0 14.0 39.0 78-79 30.736761501369575 35.0 30.5 36.0 9.5 39.0 80-81 30.289230149909425 34.0 30.0 36.0 7.0 37.5 82-83 29.869205821351372 34.0 30.0 35.0 6.5 37.0 84-85 29.509495313689627 34.0 29.0 35.0 2.0 36.5 86-87 29.19508440609439 34.0 29.0 35.0 2.0 36.0 88-89 28.829470460054782 34.0 29.0 35.0 2.0 36.0 90-91 28.457715565901513 34.0 29.0 35.0 2.0 35.5 92-93 28.064337659382687 34.0 28.0 35.0 2.0 35.0 94-95 27.616160988544575 34.0 27.0 35.0 2.0 35.0 96-97 27.120048132039308 33.5 26.0 35.0 2.0 35.0 98-99 26.484932746414163 33.0 25.0 35.0 2.0 35.0 100-101 24.19421155343969 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37234123477071535 1101 2 0.4104923918367547 1101 3 0.3640637687135637 1101 4 0.292509700223583 1101 5 0.2784950451410779 1101 6 0.19560071597991424 1101 7 0.26505686032608367 1101 8 0.29620269941111843 1101 9 0.3103011212230484 1101 10-11 0.34583223555272014 1101 12-13 0.3680665920380406 1101 14-15 0.6614820905904821 1101 16-17 0.64908323310339 1101 18-19 0.6164465577058209 1101 20-21 0.5770407193452272 1101 22-23 0.6740520615775552 1101 24-25 0.5404739092656996 1101 26-27 0.6631665751180265 1101 28-29 0.6928138879186889 1101 30-31 0.7152212512786136 1101 32-33 0.6853798671708233 1101 34-35 0.7050979350261244 1101 36-37 0.6935203786589739 1101 38-39 0.590070519437667 1101 40-41 0.6173131740022271 1101 42-43 0.6636091202637004 1101 44-45 0.7829514321971445 1101 46-47 0.6241277414464932 1101 48-49 0.6571557943424366 1101 50-51 0.5368837758589535 1101 52-53 0.5967489101172774 1101 54-55 0.7142952294856926 1101 56-57 0.8376379763739621 1101 58-59 0.6978614444587947 1101 60-61 0.8042597722562235 1101 62-63 0.8262523663043311 1101 64-65 0.865616840756914 1101 66-67 0.8583487443626012 1101 68-69 0.8899362755737528 1101 70-71 0.9055402670968462 1101 72-73 0.8142953567329343 1101 74-75 0.6905636791046241 1101 76-77 0.7983792672786159 1101 78-79 0.7899915340908485 1101 80-81 0.5400093839943381 1101 82-83 0.7306667890223828 1101 84-85 0.7512702875283601 1101 86-87 0.7219133378463631 1101 88-89 0.6364051575785155 1101 90-91 0.6678098435421873 1101 92-93 0.7955331013153248 1101 94-95 0.8252643766010017 1101 96-97 0.7965291580673259 1101 98-99 0.822279540229637 1101 100-101 0.9716994796590157 1102 1 0.25955055444772057 1102 2 0.16404015135760375 1102 3 0.18543706128434678 1102 4 0.21255276950189028 1102 5 0.16296576306808674 1102 6 0.054624000250207416 1102 7 0.16155750477977193 1102 8 0.14865619335736113 1102 9 0.182443468079029 1102 10-11 0.45014331362767024 1102 12-13 0.4079597209119825 1102 14-15 0.3233803284619725 1102 16-17 0.44312184996215365 1102 18-19 0.42479652310490934 1102 20-21 0.43662279439283935 1102 22-23 0.48705006759493585 1102 24-25 0.5068873799332678 1102 26-27 0.4471156213079084 1102 28-29 0.35764070614543897 1102 30-31 0.395735154131188 1102 32-33 0.37177088921281154 1102 34-35 0.2698429141629859 1102 36-37 0.12529171231437886 1102 38-39 0.28664549523568184 1102 40-41 0.05248491018470958 1102 42-43 0.15388603016888425 1102 44-45 0.1853008902405051 1102 46-47 0.28489277605672925 1102 48-49 0.46060045059960686 1102 50-51 0.2992683850738018 1102 52-53 0.3379412986622228 1102 54-55 0.25281360154531995 1102 56-57 0.34080610510802245 1102 58-59 0.40854855706378146 1102 60-61 0.3442418749099616 1102 62-63 0.5148106086898068 1102 64-65 0.4214416855191203 1102 66-67 0.23852936859666585 1102 68-69 0.10113270244644212 1102 70-71 0.17778706954334922 1102 72-73 0.3267120021043368 1102 74-75 0.18037799996607973 1102 76-77 0.2602665932714565 1102 78-79 0.27762977453642534 1102 80-81 0.2643805520130229 1102 82-83 0.2759006762812284 1102 84-85 0.23028996248494238 1102 86-87 0.2057361979523442 1102 88-89 0.04163665728100696 1102 90-91 0.24869670881126638 1102 92-93 0.4158159987641845 1102 94-95 0.19377474661627758 1102 96-97 0.3696655117838006 1102 98-99 0.5293233444276311 1102 100-101 0.4597268638112375 1103 1 0.25019820969198747 1103 2 0.2104322072525413 1103 3 0.30130050654570084 1103 4 0.161522536878266 1103 5 0.23702623264737355 1103 6 0.19546872746491317 1103 7 0.18710447967628596 1103 8 0.3021279307314302 1103 9 0.20861931158243863 1103 10-11 0.4119769999023788 1103 12-13 0.35432349215977155 1103 14-15 0.49063140010173356 1103 16-17 0.3862979577203376 1103 18-19 0.3741403806614798 1103 20-21 0.6267874085122713 1103 22-23 0.5963450488480078 1103 24-25 0.45857830364811036 1103 26-27 0.6198986248161518 1103 28-29 0.5461778249164126 1103 30-31 0.3652857477297218 1103 32-33 0.5248845108245206 1103 34-35 0.4593308266729892 1103 36-37 0.35480231030272336 1103 38-39 0.33444221688804276 1103 40-41 0.4370076487306065 1103 42-43 0.44444182799809795 1103 44-45 0.5473011436651731 1103 46-47 0.5029660518845631 1103 48-49 0.4672412056173769 1103 50-51 0.45784507708052047 1103 52-53 0.5407988061332034 1103 54-55 0.6361259846252167 1103 56-57 0.7787285246709601 1103 58-59 0.7336414974902183 1103 60-61 0.6538812357310775 1103 62-63 0.8331012449012292 1103 64-65 0.6108409715980159 1103 66-67 0.620491687742792 1103 68-69 0.6571113945585907 1103 70-71 0.5460010104990047 1103 72-73 0.4324919796787796 1103 74-75 0.43114919431824816 1103 76-77 0.5570655004407747 1103 78-79 0.5002609940000191 1103 80-81 0.5165951297793754 1103 82-83 0.6794428996458244 1103 84-85 0.5707016476001066 1103 86-87 0.5190742231316356 1103 88-89 0.6616999304157538 1103 90-91 0.7912996688979632 1103 92-93 0.9347242041637891 1103 94-95 0.922938510210777 1103 96-97 0.9012634517517597 1103 98-99 0.9884897541799305 1103 100-101 1.0813433557641012 1104 1 0.4146852818447151 1104 2 0.28666647903371967 1104 3 0.3350999064442597 1104 4 0.34713782418752714 1104 5 0.34585786813769204 1104 6 0.37340430516949397 1104 7 0.35317491520815736 1104 8 0.30255020170595515 1104 9 0.43370257412988167 1104 10-11 0.5231515976449685 1104 12-13 0.49619890083373974 1104 14-15 0.6137120838018291 1104 16-17 0.5691819279230401 1104 18-19 0.5967080448491089 1104 20-21 0.6045390447359082 1104 22-23 0.5121670717154245 1104 24-25 0.6241843022181328 1104 26-27 0.43897506747345716 1104 28-29 0.5089965906140748 1104 30-31 0.6283984531361284 1104 32-33 0.5120840781648326 1104 34-35 0.5562866257184496 1104 36-37 0.5329563567097466 1104 38-39 0.4882689330131669 1104 40-41 0.4420092070388293 1104 42-43 0.47511238941682876 1104 44-45 0.39058697406012044 1104 46-47 0.49109632645235735 1104 48-49 0.4425692142216846 1104 50-51 0.39116231808053215 1104 52-53 0.35889687566430695 1104 54-55 0.4465293725706303 1104 56-57 0.4377315740662695 1104 58-59 0.45060994633187335 1104 60-61 0.5164240892073266 1104 62-63 0.5693542163811287 1104 64-65 0.6387389665747705 1104 66-67 0.3045422502615125 1104 68-69 0.3658137821312337 1104 70-71 0.2866441334709151 1104 72-73 0.43268019886858866 1104 74-75 0.4957807919202928 1104 76-77 0.4065482032194545 1104 78-79 0.5300042092482151 1104 80-81 0.5794022845529909 1104 82-83 0.505060264699388 1104 84-85 0.4818738009376382 1104 86-87 0.5761828586036231 1104 88-89 0.5585729252397229 1104 90-91 0.6031981160896471 1104 92-93 0.6707935397133866 1104 94-95 0.6938861449433276 1104 96-97 0.6830786058409437 1104 98-99 0.7948109856712016 1104 100-101 0.627805996088231 1105 1 0.0013454731316429047 1105 2 0.22708736994243495 1105 3 0.1841078265005187 1105 4 0.27154443416390706 1105 5 0.18480815468202394 1105 6 0.22198685068689628 1105 7 0.21410200883750008 1105 8 0.23115510395448524 1105 9 0.20357398344187771 1105 10-11 0.288008009608987 1105 12-13 0.19350332659785607 1105 14-15 0.27273416107381365 1105 16-17 0.21759363923516872 1105 18-19 0.24550380408957295 1105 20-21 0.2066569677183807 1105 22-23 0.18496345743571396 1105 24-25 0.287805785562675 1105 26-27 0.2225626121068629 1105 28-29 0.27081738316370263 1105 30-31 -0.1156928725311559 1105 32-33 -0.031347995935277595 1105 34-35 0.12721309199054787 1105 36-37 0.17937412487097504 1105 38-39 0.037279090497385425 1105 40-41 0.08726702235275496 1105 42-43 0.20320246387931462 1105 44-45 0.25205820139465374 1105 46-47 0.052174687760121685 1105 48-49 0.27227466779878284 1105 50-51 0.22319105542973716 1105 52-53 0.118744958693064 1105 54-55 0.2296202959396041 1105 56-57 0.3754722017657173 1105 58-59 0.3086115809704211 1105 60-61 0.2980623953778476 1105 62-63 0.270214310010914 1105 64-65 0.31649322942341485 1105 66-67 0.294541956324899 1105 68-69 0.299666988431305 1105 70-71 0.20960062690362946 1105 72-73 0.35640557783842297 1105 74-75 0.33953944276000314 1105 76-77 0.43125313692358347 1105 78-79 0.3086197941184672 1105 80-81 0.4397916496630643 1105 82-83 0.5039672592062843 1105 84-85 0.3639297164558677 1105 86-87 0.49283871103515864 1105 88-89 0.5342899002546773 1105 90-91 0.4604456058929003 1105 92-93 0.4887088886007902 1105 94-95 0.4596387564824056 1105 96-97 0.3881215929739774 1105 98-99 0.5858721977517334 1105 100-101 0.38564579010447275 1106 1 0.17008611936267926 1106 2 0.26139277745272693 1106 3 0.16922299674360275 1106 4 0.11457975686669641 1106 5 0.10463805515971103 1106 6 0.11097461586737012 1106 7 0.15519551602083226 1106 8 0.18634458394773645 1106 9 0.2745901302453575 1106 10-11 0.40242968200006146 1106 12-13 0.2676622949107852 1106 14-15 0.3509170893911957 1106 16-17 0.41598645087493225 1106 18-19 0.2884213635140469 1106 20-21 0.44374513271078087 1106 22-23 0.11696106358866132 1106 24-25 0.3033639072361396 1106 26-27 0.1556662061075258 1106 28-29 0.20199302406511066 1106 30-31 0.3019115038663003 1106 32-33 0.43666310117883 1106 34-35 0.401931133055605 1106 36-37 0.2022638517857871 1106 38-39 0.3300811746158061 1106 40-41 0.450964394504453 1106 42-43 0.29004088099728875 1106 44-45 0.321582680006145 1106 46-47 0.17146256312216934 1106 48-49 0.4054385117357455 1106 50-51 0.4310801632461718 1106 52-53 0.48169927820524805 1106 54-55 0.5179998642575541 1106 56-57 0.47078203807252095 1106 58-59 0.5315140487503882 1106 60-61 0.5029133452003762 1106 62-63 0.4507036938196052 1106 64-65 0.3892468892817931 1106 66-67 0.4886788703796796 1106 68-69 0.36572572740865184 1106 70-71 0.2771670498788197 1106 72-73 0.5248326821448401 1106 74-75 0.49213727824851716 1106 76-77 0.6689894261376352 1106 78-79 0.4212331160388523 1106 80-81 0.5444072972399709 1106 82-83 0.5650936234658239 1106 84-85 0.5861392426751344 1106 86-87 0.7369352939710119 1106 88-89 0.6876720167456618 1106 90-91 0.6483945868031356 1106 92-93 0.6231408523808426 1106 94-95 0.6911781849738503 1106 96-97 0.5419379755731875 1106 98-99 0.5438989428016399 1106 100-101 0.6229598811424388 1107 1 0.23195883383416316 1107 2 0.24095287832131618 1107 3 0.285300006032994 1107 4 0.1935627730099796 1107 5 0.2477785958555927 1107 6 0.13060196246305367 1107 7 0.18823770265646544 1107 8 0.33823730689677944 1107 9 0.3075750215098765 1107 10-11 0.4622544910753703 1107 12-13 0.43587728366723866 1107 14-15 0.414933890046413 1107 16-17 0.4509212706549519 1107 18-19 0.4012956693240497 1107 20-21 0.5114193178233322 1107 22-23 0.4250454584130381 1107 24-25 0.43346050112511847 1107 26-27 0.3226303956025376 1107 28-29 0.5032668133187244 1107 30-31 0.5225272405159416 1107 32-33 0.5082327428941653 1107 34-35 0.47238135972256856 1107 36-37 0.5932928155837303 1107 38-39 0.24489975124792096 1107 40-41 0.4194152102899622 1107 42-43 0.4271698979595939 1107 44-45 0.41563024773682145 1107 46-47 0.31006850747201753 1107 48-49 0.5154881916719489 1107 50-51 0.37722718424609525 1107 52-53 0.45566436933901144 1107 54-55 0.5707116954822737 1107 56-57 0.6192415289878781 1107 58-59 0.6603223948359869 1107 60-61 0.710158605310582 1107 62-63 0.6084927211274049 1107 64-65 0.6291946948151974 1107 66-67 0.569003298683235 1107 68-69 0.6345953921139866 1107 70-71 0.42586488235361486 1107 72-73 0.5316020673780812 1107 74-75 0.5888857210357621 1107 76-77 0.5337475642356431 1107 78-79 0.6297500998955705 1107 80-81 0.5383586841830912 1107 82-83 0.47207970733618865 1107 84-85 0.45815530333997145 1107 86-87 0.44422150500477287 1107 88-89 0.46765051751079767 1107 90-91 0.4006942146526349 1107 92-93 0.5665340135766073 1107 94-95 0.40300716457741714 1107 96-97 0.39761820992741903 1107 98-99 0.4534965460949536 1107 100-101 0.43408048161229473 1108 1 0.2136683673232156 1108 2 0.23281548485692838 1108 3 0.2130067875236641 1108 4 0.30617727030904973 1108 5 0.2982809508677775 1108 6 0.10023663632488677 1108 7 0.233527074298145 1108 8 0.22104840845386775 1108 9 0.2216964191209314 1108 10-11 0.22015934836349516 1108 12-13 0.35784780675558636 1108 14-15 0.44460352944237513 1108 16-17 0.4945024485673599 1108 18-19 0.5144894480067208 1108 20-21 0.35282223300884397 1108 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70-71 -1.5303313708126254 2316 72-73 -2.0225115230425637 2316 74-75 -2.346926464462804 2316 76-77 -2.5683049797014874 2316 78-79 -2.68903415068932 2316 80-81 -2.7487225401026123 2316 82-83 -2.3319813607410893 2316 84-85 -2.6877452996435203 2316 86-87 -3.2945810013119683 2316 88-89 -3.322995871707434 2316 90-91 -2.9579189548019897 2316 92-93 -2.945657861902923 2316 94-95 -3.4277377844489187 2316 96-97 -3.4196531834802286 2316 98-99 -3.238782973092796 2316 100-101 -3.1299246293628045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7045.0 3 2780.0 4 2759.0 5 3087.0 6 3441.0 7 3985.0 8 4961.0 9 6040.0 10 7662.0 11 9192.0 12 11127.0 13 13154.0 14 14625.0 15 17123.0 16 20268.0 17 25297.0 18 23229.0 19 17456.0 20 17507.0 21 18798.0 22 20660.0 23 23171.0 24 26270.0 25 30144.0 26 35263.0 27 41407.0 28 49144.0 29 59196.0 30 71462.0 31 86889.0 32 109535.0 33 140323.0 34 187820.0 35 262031.0 36 384970.0 37 549566.0 38 471562.0 39 77747.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09661109659779 18.699691204763667 14.36377605656787 22.83992164207067 2 15.024598542472011 20.41095971960218 40.250387213870574 24.314054524055233 3 19.460459338198373 24.70978865008743 29.810239556978203 26.019512454735995 4 12.620906005342627 16.613658298792096 37.82647247930678 32.938963216558506 5 13.775187632030706 37.43978384005453 34.051461282843704 14.733567245071058 6 31.342613349773018 36.2319607128846 17.826563638401613 14.598862298940762 7 28.08897653516223 32.811962548731834 21.053938653057365 18.045122263048572 8 26.056386130134285 34.211102435818894 20.1219258027827 19.61058563126412 9 26.090334413238242 17.10404041359342 20.844126252547827 35.961498920620514 10-11 24.668999299229398 26.57115098550135 27.601814472165252 21.158035243103996 12-13 26.037610206402857 24.076707754810656 27.854647523414428 22.03103451537206 14-15 22.799589063743454 25.222105932725825 26.66027774975621 25.318027253774506 16-17 22.04649426281699 28.748992201689948 26.849025333825526 22.355488201667534 18-19 21.900690556556786 27.518913432827457 29.02839834165564 21.551997668960116 20-21 22.869418801458053 26.316787660311835 28.979091492560443 21.834702045669665 22-23 22.449302235518033 26.34872624651573 29.02141396117226 22.18055755679398 24-25 22.601625845474306 26.470860086190328 28.888831704091782 22.038682364243584 26-27 22.288332523730126 26.59676946439278 29.107694731452426 22.00720328042467 28-29 21.885604991980774 26.971467243364668 29.060194429010515 22.082733335644047 30-31 22.177024933079302 26.45284252788333 28.957686094849187 22.412446444188173 32-33 21.993487453905345 26.666028353363476 29.00789133783758 22.33259285489359 34-35 22.580507385003767 26.520302174339616 28.762296159489964 22.136894281166654 36-37 22.43074769334013 26.313819865149252 28.89625671107489 22.35917573043573 38-39 22.11746238071641 26.787054909004283 28.915338298301002 22.180144411978304 40-41 22.37876490101928 26.60615238983433 28.829805259273883 22.1852774498725 42-43 22.10909031079468 26.750172801794026 28.41689402055908 22.723842866852216 44-45 22.33309039533783 26.671038850476215 28.36652902513953 22.629341729046423 46-47 22.69646499961931 26.952870453914947 28.048122843297957 22.302541703167787 48-49 22.629335467264838 27.026228524601297 27.782258473853915 22.56217753427995 50-51 22.1259742460183 27.20581130923542 28.120807906550798 22.547406538195485 52-53 22.73144888138343 27.355936884809896 27.39213393328016 22.520480300526522 54-55 22.367559214886175 26.990834597408092 27.69673268328442 22.944873504421317 56-57 22.569686856429005 27.251890142409284 27.4956462126777 22.682776788484013 58-59 22.947806391082732 26.95110765697642 27.4752842659497 22.62580168599115 60-61 22.693325462647856 26.947854607992245 28.088176185714563 22.270643743645334 62-63 22.396358393129066 27.126655735357524 28.032226064187803 22.444759807325614 64-65 22.31024348985023 27.349692020864406 27.751415352692955 22.58864913659241 66-67 22.443321413898143 27.330359864010955 27.333423311152227 22.892895410938678 68-69 22.165574920797653 27.2147770149329 27.809634954915584 22.81001310935386 70-71 22.50242630747513 27.043927719144932 27.690470388545307 22.763175584834634 72-73 22.507596607951307 26.82771113738525 27.914308791828606 22.75038346283483 74-75 22.210084855945507 27.604467274363586 27.46126969338737 22.724178176303532 76-77 22.39898515086357 27.162725652846948 27.475681821126187 22.962607375163298 78-79 22.40785898345255 27.23541669256574 27.556028226981972 22.80069609699974 80-81 22.519803266427203 27.398136871717693 27.412966123476018 22.669093738379082 82-83 22.430980917056296 27.20764333217488 27.7024745060311 22.658901244737724 84-85 22.355250984075354 27.39336667124749 27.3611549985426 22.890227346134555 86-87 22.326654360531478 27.312109685199164 27.5707906225009 22.790445331768456 88-89 22.378343801413305 27.212590546061595 27.224372169040556 23.184693483484544 90-91 22.465468725874675 27.028505566352813 27.506924460966907 22.99910124680561 92-93 22.691614579020175 27.056489615064688 27.416978556423 22.834917249492136 94-95 22.418211073088997 26.896734395124238 27.489591089065406 23.19546344272136 96-97 22.045391711564445 26.979288628679914 27.817135377243773 23.158184282511865 98-99 22.223953978101072 27.11647760854242 27.303927534772537 23.35564087858397 100-101 22.187068729990823 26.930355804325135 27.430103609883222 23.452471855800823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 388.0 1 307.5 2 275.0 3 337.0 4 319.5 5 340.5 6 559.0 7 1140.0 8 2011.0 9 2470.5 10 2313.0 11 2219.5 12 2411.0 13 2652.5 14 2866.5 15 3096.5 16 3229.5 17 3345.0 18 3595.5 19 3851.0 20 4247.0 21 4902.5 22 5887.5 23 7197.5 24 8808.0 25 11338.5 26 14949.0 27 18721.5 28 21828.0 29 26121.0 30 32159.5 31 38068.0 32 43700.0 33 49354.5 34 55948.0 35 61179.5 36 67355.0 37 75139.0 38 79729.0 39 81721.0 40 83682.5 41 87578.5 42 90575.5 43 93653.0 44 98724.5 45 102408.5 46 103656.5 47 111264.5 48 128543.5 49 128482.0 50 112758.0 51 104142.5 52 99450.5 53 96504.5 54 92648.5 55 85496.5 56 77814.0 57 70465.5 58 65207.0 59 60550.5 60 52817.0 61 42546.5 62 32393.5 63 24303.5 64 18365.5 65 14155.5 66 10228.5 67 7594.0 68 5349.0 69 3274.0 70 2163.0 71 1358.5 72 874.5 73 594.5 74 405.0 75 272.5 76 178.0 77 104.5 78 51.5 79 32.0 80 24.5 81 22.0 82 17.5 83 15.5 84 19.0 85 15.5 86 11.0 87 12.5 88 9.5 89 4.5 90 4.5 91 3.5 92 2.5 93 2.0 94 1.5 95 2.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03889068776308535 2 0.0373854676246401 3 0.02460859900760486 4 0.04204114851797076 5 0.06307922533670549 6 0.06458444547515074 7 0.06353429189018894 8 0.0655995939406138 9 0.06454944035565202 10-11 0.07037779275219 12-13 0.06889007517349412 14-15 0.06234411782723223 16-17 0.0521926331726015 18-19 0.04715189596478485 20-21 0.05417042242427955 22-23 0.029911874611661955 24-25 0.017082498315378624 26-27 0.013564483805756592 28-29 0.016242375447409186 30-31 0.012566837900042881 32-33 0.008348721000446315 34-35 0.01030900769237501 36-37 0.005583316560046907 38-39 0.008558751717438674 40-41 0.007316069975233878 42-43 0.0044981578555863795 44-45 0.0010151484654630741 46-47 0.0027128967611513184 48-49 9.101331069668939E-4 50-51 0.00549580376130009 52-53 0.004848209050573647 54-55 0.007771136528717325 56-57 0.020688025623747473 58-59 0.008576254277188038 60-61 0.019497851560790765 62-63 0.01412456571773622 64-65 0.009853941138891563 66-67 0.016294883126657275 68-69 0.02621883450454629 70-71 0.018657728692821327 72-73 0.01806264166134297 74-75 0.018605221013573234 76-77 0.02569375771206539 78-79 0.038103072574364 80-81 0.03090952051737567 82-83 0.03159212034760084 84-85 0.02161566129046373 86-87 0.024398568290612502 88-89 0.02037297954825893 90-91 0.019112795246304774 92-93 0.030857012838127574 94-95 0.02235076879993699 96-97 0.03750798554288565 98-99 0.054695499216760445 100-101 0.062484138305227144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2856725.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.708090827037 #Duplication Level Percentage of deduplicated Percentage of total 1 74.53045501259223 37.79297082158922 2 13.694727360441433 13.888669576895458 3 4.76474288205301 7.248330444918663 4 2.2439060931827606 4.551367759218127 5 1.27281114620577 3.227091160373363 6 0.7634933402768161 2.3229173786756796 7 0.5080256752673912 1.8032708458747975 8 0.3509759524337963 1.4237856379295009 9 0.2629332348243536 1.1999558117627973 >10 1.4249864212026628 13.17692244813915 >50 0.11315831952284136 3.963061616013176 >100 0.06500752623470618 5.8833252439169135 >500 0.0031154581059301906 1.0412918044246515 >1k 0.0013846480470281325 1.3939709948100742 >5k 2.769296094056265E-4 1.0830684554583727 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9583 0.33545406015629786 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8788 0.30762499015481015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7003 0.245140851849583 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5477 0.19172303949452607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.15944831931670006 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4378 0.15325241316542546 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3354 0.11740717079872932 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2932 0.10263501037026665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.500511949872669E-5 2 0.0 0.0 0.0 0.0 3.500511949872669E-5 3 0.0 0.0 0.0 0.0 3.500511949872669E-5 4 0.0 0.0 0.0 0.0 3.500511949872669E-5 5 0.0 0.0 0.0 0.0 3.500511949872669E-5 6 0.0 0.0 0.0 0.0 7.001023899745338E-5 7 0.0 0.0 0.0 0.0 1.0501535849618007E-4 8 0.0 0.0 0.0 0.0 1.0501535849618007E-4 9 0.0 0.0 0.0 0.0 1.0501535849618007E-4 10-11 0.0 0.0 0.0 0.0 1.0501535849618007E-4 12-13 0.0 0.0 0.0 0.0 3.150460754885402E-4 14-15 0.0 0.0 0.0 0.0 3.500511949872669E-4 16-17 0.0 0.0 0.0 0.0 3.8505631448599356E-4 18-19 5.2507679248090036E-5 0.0 0.0 0.0 3.8505631448599356E-4 20-21 1.0501535849618007E-4 0.0 0.0 0.0 4.7256911323281033E-4 22-23 1.0501535849618007E-4 0.0 0.0 3.500511949872669E-5 4.900716729821737E-4 24-25 1.4002047799490676E-4 0.0 0.0 1.0501535849618007E-4 4.900716729821737E-4 26-27 1.4002047799490676E-4 0.0 0.0 3.6755375473663024E-4 4.900716729821737E-4 28-29 1.575230377442701E-4 0.0 0.0 0.0011726715032073442 5.07574232731537E-4 30-31 1.7502559749363344E-4 0.0 0.0 0.0028354146793968618 5.425793522302637E-4 32-33 1.7502559749363344E-4 0.0 0.0 0.004515660415335743 5.60081911979627E-4 34-35 1.925281572429968E-4 0.0 0.0 0.00607338823302908 5.950870314783537E-4 36-37 2.2753327674172348E-4 0.0 0.0 0.008383726119945041 5.950870314783537E-4 38-39 2.4503583649108685E-4 0.0 0.0 0.011761720151572168 5.950870314783537E-4 40-41 2.4503583649108685E-4 1.7502559749363345E-5 0.0 0.015927329371920644 5.950870314783537E-4 42-43 2.4503583649108685E-4 3.500511949872669E-5 0.0 0.020075436032519756 5.950870314783537E-4 44-45 2.800409559898135E-4 3.500511949872669E-5 0.0 0.022088230403696542 6.12589591227717E-4 46-47 3.150460754885402E-4 3.500511949872669E-5 0.0 0.02495865020259213 6.300921509770804E-4 48-49 3.150460754885402E-4 3.500511949872669E-5 0.0 0.02900174150469506 6.825998302251704E-4 50-51 3.150460754885402E-4 3.500511949872669E-5 0.0 0.034515047825744515 7.001023899745338E-4 52-53 3.150460754885402E-4 3.500511949872669E-5 0.0 0.045786696304334504 7.001023899745338E-4 54-55 3.150460754885402E-4 3.500511949872669E-5 0.0 0.05352282771355311 7.001023899745338E-4 56-57 3.150460754885402E-4 3.500511949872669E-5 0.0 0.0742458584567993 7.351075094732605E-4 58-59 3.150460754885402E-4 3.500511949872669E-5 0.0 0.11322405901863147 7.701126289719871E-4 60-61 3.150460754885402E-4 3.500511949872669E-5 0.0 0.1448686870454804 7.876151887213505E-4 62-63 3.150460754885402E-4 3.500511949872669E-5 0.0 0.16067349849915552 8.751279874681672E-4 64-65 3.150460754885402E-4 3.500511949872669E-5 0.0 0.19326326475247005 8.751279874681672E-4 66-67 3.150460754885402E-4 3.500511949872669E-5 0.0 0.25758517183138036 8.751279874681672E-4 68-69 3.150460754885402E-4 3.500511949872669E-5 0.0 0.35271158406917014 9.101331069668939E-4 70-71 3.850563144859936E-4 3.500511949872669E-5 0.0 0.43455355345719315 9.101331069668939E-4 72-73 4.5506655348344696E-4 3.500511949872669E-5 0.0 0.5251468027198978 9.626407862149839E-4 74-75 5.07574232731537E-4 3.500511949872669E-5 0.0 0.6323324786249989 0.001015148465463074 76-77 5.60081911979627E-4 3.500511949872669E-5 0.0 0.7018176408299714 0.001015148465463074 78-79 5.60081911979627E-4 3.500511949872669E-5 0.0 0.7944411870236022 0.001015148465463074 80-81 5.60081911979627E-4 3.500511949872669E-5 0.0 0.9213697503259852 0.001015148465463074 82-83 5.775844717289904E-4 3.500511949872669E-5 0.0 1.030673235960759 0.001015148465463074 84-85 5.950870314783537E-4 3.500511949872669E-5 0.0 1.1876361917930498 0.001015148465463074 86-87 5.950870314783537E-4 3.500511949872669E-5 0.0 1.4148894275787836 0.001067656144711164 88-89 6.12589591227717E-4 3.500511949872669E-5 0.0 1.704574293990496 0.0011376663837086175 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12635 0.0 25.956482 1 TACATGG 12655 0.0 25.08917 2 ACATGGG 12585 0.0 24.322298 3 GTATCAA 20785 0.0 21.90723 1 CATGGGG 6135 0.0 21.15047 4 GAGTACT 8685 0.0 20.791367 12-13 AGAGTAC 15045 0.0 20.642424 10-11 AGTACTT 9255 0.0 19.459517 12-13 CATGGGA 7225 0.0 19.406898 4 GTACTTT 9480 0.0 19.046785 14-15 ATCAACG 23745 0.0 18.896084 3 CAACGCA 23680 0.0 18.868658 5 TCAACGC 23690 0.0 18.859701 4 TATCAAC 24075 0.0 18.716043 2 AACGCAG 24075 0.0 18.577192 6 GGTATCA 16050 0.0 17.916466 1 GAGTACA 9980 0.0 17.09763 1 ACCGTAT 195 2.2665923E-4 17.06132 8 CGCGGAA 350 0.0 16.965054 82-83 ACGCAGA 26145 0.0 16.942755 7 >>END_MODULE