FastQCFastQC Report
Thu 26 May 2016
SRR937106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856725
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109640.3837961301840394No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104140.36454331445973975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86160.3016041096010291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56170.1966237562243478No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49140.17201515721674293No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41090.14383603602026795No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29710.10400021003071698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119650.022.002161
GAGTACT108550.021.50491112-13
TACATGG122700.021.4108922
ACATGGG121250.020.5281833
GTATCAA242050.020.4957451
GTACTTT115100.019.99368514-15
AGAGTAC175050.019.90010510-11
TAGGCCG1200.004769076619.7888855
GGACCGT2651.8613719E-819.713526
AGTACTT112800.019.64203812-13
GGTACCG1759.9540805E-518.997335
GTACCGA1801.2357967E-418.4689796
CAACGCA275400.017.9177785
TCAACGC275250.017.910294
ATCAACG275750.017.880633
TATCAAC278750.017.7585322
CATGGGG58800.017.6888394
AACGCAG280400.017.6654076
GGTATCA192500.017.452471
CGGTTAC4000.017.22537428-29
Produced by FastQC (version 0.11.5)