##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937106_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2856725 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11462496390097 31.0 31.0 34.0 31.0 34.0 2 32.195795535097005 33.0 31.0 34.0 30.0 34.0 3 32.33480296493362 34.0 31.0 34.0 30.0 34.0 4 36.01781690572246 37.0 35.0 37.0 35.0 37.0 5 35.91970525689382 37.0 35.0 37.0 35.0 37.0 6 35.94901259309174 37.0 35.0 37.0 35.0 37.0 7 35.89877919645748 37.0 35.0 37.0 35.0 37.0 8 35.88072005530809 37.0 35.0 37.0 35.0 37.0 9 37.55870761098811 39.0 37.0 39.0 35.0 39.0 10-11 37.45424078271447 39.0 37.0 39.0 34.5 39.0 12-13 37.38139600416561 39.0 37.0 39.0 34.0 39.0 14-15 38.5882472061539 40.0 38.0 41.0 34.0 41.0 16-17 38.517022639561034 40.0 38.0 41.0 34.0 41.0 18-19 38.54334228880974 40.0 38.0 41.0 34.0 41.0 20-21 38.62950283979032 40.0 38.0 41.0 34.0 41.0 22-23 38.56470626329101 40.0 38.0 41.0 34.0 41.0 24-25 38.477913344826675 40.0 38.0 41.0 34.0 41.0 26-27 38.30742126035933 40.0 38.0 41.0 34.0 41.0 28-29 38.16046399285896 40.0 38.0 41.0 33.5 41.0 30-31 38.13172986549283 40.0 38.0 41.0 34.0 41.0 32-33 38.05995046775591 40.0 37.0 41.0 33.0 41.0 34-35 37.944734092361 40.0 37.0 41.0 33.0 41.0 36-37 37.77882452808723 40.0 37.0 41.0 33.0 41.0 38-39 37.65076950003938 40.0 37.0 41.0 33.0 41.0 40-41 37.45234700574959 40.0 37.0 41.0 32.5 41.0 42-43 37.234353674224856 40.0 36.5 41.0 31.5 41.0 44-45 37.01067673647271 40.0 36.0 41.0 31.0 41.0 46-47 36.68995248055028 39.0 36.0 41.0 30.5 41.0 48-49 36.340988369549045 39.0 36.0 41.0 30.0 41.0 50-51 36.10573208831792 39.0 35.0 40.5 29.5 41.0 52-53 35.89262879696156 39.0 35.0 40.0 29.0 41.0 54-55 35.62711531561491 38.5 35.0 40.0 28.0 41.0 56-57 35.36140493047108 38.0 34.5 40.0 28.0 41.0 58-59 35.015103483884516 38.0 34.0 40.0 27.0 41.0 60-61 34.66121625287698 38.0 34.0 40.0 26.0 41.0 62-63 34.33305550936825 37.0 34.0 40.0 26.0 41.0 64-65 33.92595734626189 36.5 33.0 39.0 26.0 41.0 66-67 33.48326545257244 36.0 33.0 39.0 25.0 40.0 68-69 33.04436181291514 35.5 32.0 39.0 23.5 40.0 70-71 32.635867645643174 35.0 32.0 38.0 23.0 40.0 72-73 32.23904821080083 35.0 31.5 37.5 22.0 39.5 74-75 31.77076722470661 35.0 31.0 37.0 21.0 39.0 76-77 30.29718926392985 33.5 29.5 35.5 20.0 37.5 78-79 31.012594141893253 34.0 31.0 36.0 20.0 38.0 80-81 30.713357078472725 34.0 31.0 35.5 18.0 37.0 82-83 30.510263851088222 34.0 30.0 35.0 18.0 37.0 84-85 30.17877149533119 34.0 30.0 35.0 17.0 36.5 86-87 29.831366162301236 34.0 30.0 35.0 11.5 36.0 88-89 29.452401963787203 34.0 29.5 35.0 7.0 36.0 90-91 28.998362985586642 34.0 29.0 35.0 2.0 35.0 92-93 28.560384356212097 34.0 29.0 35.0 2.0 35.0 94-95 28.065194584708014 33.0 28.5 35.0 2.0 35.0 96-97 27.421752181256508 33.0 27.0 35.0 2.0 35.0 98-99 26.64316271254671 33.0 25.5 34.5 2.0 35.0 100-101 23.46743928799587 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3107009172448798 1101 2 0.24567164483969606 1101 3 0.1886722731611954 1101 4 0.150569183374202 1101 5 0.20697142318380202 1101 6 0.23189582590200786 1101 7 0.18906656105943398 1101 8 0.1992111753599417 1101 9 0.3008666060809233 1101 10-11 0.32076772018061206 1101 12-13 0.25287263817932626 1101 14-15 0.41673131697758237 1101 16-17 0.2641237016068132 1101 18-19 0.33909923524099383 1101 20-21 0.27657371385537033 1101 22-23 0.27450390164293736 1101 24-25 0.34820112218454824 1101 26-27 0.23566437959200215 1101 28-29 0.31872168349588037 1101 30-31 0.23490568864157524 1101 32-33 0.30212972084570566 1101 34-35 0.27400743181352993 1101 36-37 0.30387107416328973 1101 38-39 0.22579525203976658 1101 40-41 0.3240214512110313 1101 42-43 0.2872668327421266 1101 44-45 0.34539072819072203 1101 46-47 0.40048190729918787 1101 48-49 0.401333769907005 1101 50-51 0.26868344618272033 1101 52-53 0.40200099421885227 1101 54-55 0.45834617947404155 1101 56-57 0.5279194483980802 1101 58-59 0.5192996982841365 1101 60-61 0.5113358979882037 1101 62-63 0.46497188283180435 1101 64-65 0.5716275277089196 1101 66-67 0.6083730436699142 1101 68-69 0.5108897692062655 1101 70-71 0.525404906134419 1101 72-73 0.5073912755817815 1101 74-75 0.5564648035029407 1101 76-77 0.43228668160472594 1101 78-79 0.6989699197893238 1101 80-81 0.9988111993205955 1101 82-83 0.8310025538031987 1101 84-85 0.7837536975325534 1101 86-87 0.6993598068927263 1101 88-89 0.6080492816568253 1101 90-91 0.40762665385770447 1101 92-93 0.5223110272987981 1101 94-95 0.6964015091716576 1101 96-97 0.6555962404558109 1101 98-99 0.9437786180575714 1101 100-101 1.1288157032158743 1102 1 0.22371962785253174 1102 2 0.13881236330762903 1102 3 0.1145133738246713 1102 4 0.12076389978660274 1102 5 0.12561973053691844 1102 6 0.16116260361491896 1102 7 0.1499512066472093 1102 8 0.15983029856717224 1102 9 0.18658174449390685 1102 10-11 0.2033674706063664 1102 12-13 0.26297470621349817 1102 14-15 0.2173635997239458 1102 16-17 0.25441707259390256 1102 18-19 0.2387756094382283 1102 20-21 0.12624617096795276 1102 22-23 0.16177981175889755 1102 24-25 0.24572673712020787 1102 26-27 0.2106344418736441 1102 28-29 0.21019885342087719 1102 30-31 0.29971683852975417 1102 32-33 0.18758553892883612 1102 34-35 0.21194719451281685 1102 36-37 0.20499369196961226 1102 38-39 0.16813268836382633 1102 40-41 0.3784365185569172 1102 42-43 0.3731279758220367 1102 44-45 0.2876981801644689 1102 46-47 0.37889252222552017 1102 48-49 0.3382111632923568 1102 50-51 0.3194369253932621 1102 52-53 0.2811511920316292 1102 54-55 0.3739143992336835 1102 56-57 0.33770003569964047 1102 58-59 0.44542758550193895 1102 60-61 0.3875417072112626 1102 62-63 0.2278818943405696 1102 64-65 0.25502398517217983 1102 66-67 0.3268040460891797 1102 68-69 0.3343111840945383 1102 70-71 0.2365336700330758 1102 72-73 0.3824202806609378 1102 74-75 0.495591163057739 1102 76-77 0.5211564721153046 1102 78-79 0.3984615847927806 1102 80-81 0.5353925263915684 1102 82-83 0.47182547732864677 1102 84-85 0.3519361516047397 1102 86-87 0.33083064419755104 1102 88-89 0.28622986112421245 1102 90-91 0.25480043065475755 1102 92-93 0.34701234959465665 1102 94-95 0.4740830334025574 1102 96-97 0.47292206542181603 1102 98-99 0.6506720341843959 1102 100-101 1.0075873427769402 1103 1 0.1752927740800061 1103 2 0.1189764684117165 1103 3 0.12029142002882764 1103 4 0.10506673287216728 1103 5 0.12594427294656185 1103 6 0.12523839419517202 1103 7 0.1957808186331107 1103 8 0.13886392029440486 1103 9 0.14153749625903345 1103 10-11 0.13139974001339283 1103 12-13 0.20579585699250913 1103 14-15 0.20899147051635225 1103 16-17 0.18618176021370658 1103 18-19 0.3180724390307148 1103 20-21 0.23299517297297 1103 22-23 0.2112295697780624 1103 24-25 0.2728989510914843 1103 26-27 0.3130188177739015 1103 28-29 0.15482285714066535 1103 30-31 0.21958013361352613 1103 32-33 0.21537064708918052 1103 34-35 0.2949518940601905 1103 36-37 0.408802715187214 1103 38-39 0.3190445478438164 1103 40-41 0.46064204595818836 1103 42-43 0.4130274687561126 1103 44-45 0.4547347253004119 1103 46-47 0.4333719655865522 1103 48-49 0.4001074295745326 1103 50-51 0.42079108672007237 1103 52-53 0.43299010925129267 1103 54-55 0.3710788046929281 1103 56-57 0.32376258177275474 1103 58-59 0.359237207985835 1103 60-61 0.34533937108351154 1103 62-63 0.4416233051578331 1103 64-65 0.33390972169572564 1103 66-67 0.2883591276868671 1103 68-69 0.3125485217060131 1103 70-71 0.2923426001116951 1103 72-73 0.32440345138612514 1103 74-75 0.17085994843159824 1103 76-77 0.3638371563185743 1103 78-79 0.3163885457834361 1103 80-81 0.5770020942916894 1103 82-83 0.4364749029355117 1103 84-85 0.3191999666783758 1103 86-87 0.3137449664454195 1103 88-89 0.2876472522653266 1103 90-91 0.17778758180777032 1103 92-93 0.2856761806968642 1103 94-95 0.44596110870361727 1103 96-97 0.6039736622498921 1103 98-99 0.7592520474260134 1103 100-101 0.8854778098941871 1104 1 0.1674554003488069 1104 2 0.09420486750973112 1104 3 0.09522343501303254 1104 4 0.16538242778597123 1104 5 0.01852611468355292 1104 6 0.07946690940242718 1104 7 0.11779879723234643 1104 8 0.15795105722386893 1104 9 0.20702127457593633 1104 10-11 0.1406496743200023 1104 12-13 0.16482946145110589 1104 14-15 0.19092609490296297 1104 16-17 0.15856670512292936 1104 18-19 0.13449242448650978 1104 20-21 0.1650810449228075 1104 22-23 0.1529060649900842 1104 24-25 0.17390665707844022 1104 26-27 0.21349119905220704 1104 28-29 0.16546121352269694 1104 30-31 0.2450471677557644 1104 32-33 0.20269243468260356 1104 34-35 0.1965288408760486 1104 36-37 0.24929031288702674 1104 38-39 0.3658649879589504 1104 40-41 0.2747288467819473 1104 42-43 0.28808598170228805 1104 44-45 0.34930356280247565 1104 46-47 0.33948756150195436 1104 48-49 0.4224282088508673 1104 50-51 0.42765833719214896 1104 52-53 0.3197252396297472 1104 54-55 0.2810868101195041 1104 56-57 0.24508909928997014 1104 58-59 0.23309735135019594 1104 60-61 0.36851416437851015 1104 62-63 0.33730801357086193 1104 64-65 0.3793729058999773 1104 66-67 0.21145906107683032 1104 68-69 0.22322323861335036 1104 70-71 0.3602408395289487 1104 72-73 0.3850888625656097 1104 74-75 0.35793485111371837 1104 76-77 0.3253692483015378 1104 78-79 0.5211927183828742 1104 80-81 0.5266586553804267 1104 82-83 0.38422513362624144 1104 84-85 0.2240056670309798 1104 86-87 0.23506292854491662 1104 88-89 0.2690422742344154 1104 90-91 0.15384677307635286 1104 92-93 0.3163733250206491 1104 94-95 0.3759270703631259 1104 96-97 0.4134249544235189 1104 98-99 0.5497384229232978 1104 100-101 0.5392131663801116 1105 1 0.10217383320161844 1105 2 0.03852897342694206 1105 3 0.02239787498700707 1105 4 0.06747477389589562 1105 5 0.04121214256014838 1105 6 0.07135952338951057 1105 7 0.05917546471940227 1105 8 0.0525252944254504 1105 9 -0.044972515025989424 1105 10-11 0.07727176352493359 1105 12-13 0.0953638601511102 1105 14-15 0.16794920139180647 1105 16-17 0.1698094735144835 1105 18-19 0.1750496230161218 1105 20-21 0.14366745548733206 1105 22-23 -0.004004538356497278 1105 24-25 0.1318835922671795 1105 26-27 0.1488662512040193 1105 28-29 0.1319354613849697 1105 30-31 0.09303815486948963 1105 32-33 0.08653144867003704 1105 34-35 0.05821113224794772 1105 36-37 0.07781357095064578 1105 38-39 0.21738431122034996 1105 40-41 0.35448122053946207 1105 42-43 0.25877264834263514 1105 44-45 0.32802663195698756 1105 46-47 0.38088800714496074 1105 48-49 0.48793040394317444 1105 50-51 0.5775517522661886 1105 52-53 0.5455563454061263 1105 54-55 0.36592806021876356 1105 56-57 0.2750603680827197 1105 58-59 0.44647725828196627 1105 60-61 0.43454127761825134 1105 62-63 0.3828414712349968 1105 64-65 0.49544272769103515 1105 66-67 0.38907670920661985 1105 68-69 0.5009524071719582 1105 70-71 0.42816757297625685 1105 72-73 0.42416005796195577 1105 74-75 0.391379573262455 1105 76-77 0.23473485850736608 1105 78-79 0.446418577961019 1105 80-81 0.7053277191659895 1105 82-83 0.6359670151627661 1105 84-85 0.28861907677028853 1105 86-87 0.2536379238397366 1105 88-89 0.276884897938956 1105 90-91 0.21316133921494185 1105 92-93 0.33991698974338647 1105 94-95 0.38263237872676115 1105 96-97 0.5780366531885015 1105 98-99 0.7084070304490382 1105 100-101 0.7771705613646738 1106 1 0.09921454295753307 1106 2 0.06376100073187274 1106 3 0.0728260398358529 1106 4 0.08962387073005829 1106 5 0.06593238187307549 1106 6 0.14077606354477012 1106 7 0.09472579831473382 1106 8 0.06070492985725906 1106 9 0.06586134768132013 1106 10-11 0.09950372084483661 1106 12-13 -0.008190527192724062 1106 14-15 0.09573738720028047 1106 16-17 0.12267298295886775 1106 18-19 0.13980542908220883 1106 20-21 0.051959560457703446 1106 22-23 0.11865854471753323 1106 24-25 0.12366999614901175 1106 26-27 0.13626033154683626 1106 28-29 0.18326046649925587 1106 30-31 0.039339580410803876 1106 32-33 0.137133364824372 1106 34-35 0.18921320129267372 1106 36-37 0.12329509490168533 1106 38-39 0.00475035268738111 1106 40-41 0.12269693415353089 1106 42-43 0.12112446849185687 1106 44-45 0.028009180560701452 1106 46-47 0.20091826221349152 1106 48-49 0.1850892358750187 1106 50-51 -0.06559693721919047 1106 52-53 -0.007667542101010838 1106 54-55 0.19999062863362127 1106 56-57 0.13052835262141116 1106 58-59 0.23337260553334715 1106 60-61 0.12674162503113706 1106 62-63 0.24214858350893564 1106 64-65 0.24639874119994687 1106 66-67 0.21367595516274207 1106 68-69 0.3189886271187703 1106 70-71 0.3257866013686552 1106 72-73 0.26904693343524855 1106 74-75 0.24948958255790643 1106 76-77 0.09115100566974377 1106 78-79 0.1782610506219946 1106 80-81 0.5698371868849428 1106 82-83 0.39170224383437713 1106 84-85 0.25772508076840595 1106 86-87 0.290240634952287 1106 88-89 0.2592457981977212 1106 90-91 0.3950002677620148 1106 92-93 0.5354222009366865 1106 94-95 0.5440368437719272 1106 96-97 0.5966772567107235 1106 98-99 0.6154429937840575 1106 100-101 0.6591539013376142 1107 1 0.051065237327144075 1107 2 0.03633160515312994 1107 3 -0.0035391705584970623 1107 4 0.032652335214109485 1107 5 0.09185126312258518 1107 6 0.041853719427692226 1107 7 0.046307258322919154 1107 8 0.11506835852721053 1107 9 0.13706027577293867 1107 10-11 0.008418359722348612 1107 12-13 0.11635209768962795 1107 14-15 0.11836959761618004 1107 16-17 0.19127251525622313 1107 18-19 0.13572560351877883 1107 20-21 0.14823311621920965 1107 22-23 0.05134465588851356 1107 24-25 0.13465714689869657 1107 26-27 0.1840033609093652 1107 28-29 0.1467962734120576 1107 30-31 0.1154370831228988 1107 32-33 0.2396039807406325 1107 34-35 0.1120136158309748 1107 36-37 0.1675611573626199 1107 38-39 0.13868977765019963 1107 40-41 0.35547985474465804 1107 42-43 0.20165266356194422 1107 44-45 0.22873273668142247 1107 46-47 0.31385228812033006 1107 48-49 0.2969458753129075 1107 50-51 0.22630708315568882 1107 52-53 0.1651746316027527 1107 54-55 0.25452226142596857 1107 56-57 0.25204195623084047 1107 58-59 0.3095651215724615 1107 60-61 0.29028686143291793 1107 62-63 0.37123128282139106 1107 64-65 0.21015627581065388 1107 66-67 0.23986002956411312 1107 68-69 0.29449171280678144 1107 70-71 0.22007224124478597 1107 72-73 0.15488958849196877 1107 74-75 0.20156407013253386 1107 76-77 0.3851474745506316 1107 78-79 0.41444909438919453 1107 80-81 0.5120451062188067 1107 82-83 0.542457231585022 1107 84-85 0.45298752589776115 1107 86-87 0.5492918667129594 1107 88-89 0.26572286927271804 1107 90-91 0.25818314237316287 1107 92-93 0.42565351977195576 1107 94-95 0.49837629459849 1107 96-97 0.41641165045744444 1107 98-99 0.5071433371970642 1107 100-101 0.5519296555171209 1108 1 0.046065297726151755 1108 2 0.010226605323346405 1108 3 0.003440209220578083 1108 4 0.007248512945324137 1108 5 -0.02268007902021907 1108 6 0.040432146439883354 1108 7 0.04527772979756861 1108 8 0.0802697452884118 1108 9 0.17471850744375672 1108 10-11 0.06842202758930682 1108 12-13 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-0.7627335153523305 2316 92-93 -0.6596948383256063 2316 94-95 -0.6437355017398829 2316 96-97 -0.9130010642684034 2316 98-99 -0.8825812230379633 2316 100-101 -1.0826743698762478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 20.0 5 54.0 6 140.0 7 315.0 8 757.0 9 1445.0 10 2417.0 11 3973.0 12 5899.0 13 8368.0 14 10501.0 15 14253.0 16 18843.0 17 25875.0 18 23409.0 19 15434.0 20 13914.0 21 14368.0 22 15438.0 23 16960.0 24 20070.0 25 24151.0 26 29511.0 27 37010.0 28 46031.0 29 57539.0 30 71351.0 31 89775.0 32 113627.0 33 146517.0 34 197262.0 35 278198.0 36 423743.0 37 632146.0 38 462289.0 39 35103.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96239624785318 19.529861185273628 14.905189664439163 22.602552902434027 2 15.363153133725604 21.208589704453225 39.40969061828732 24.018566543533844 3 19.753365191058833 24.835407586339684 29.94566682420487 25.465560398396615 4 13.014370681890657 17.268446774078935 37.31970137769938 32.397481166331026 5 13.752775602493465 37.72288398707968 33.92152943355129 14.602810976875553 6 31.38390989682241 36.77487332522381 17.283707742257302 14.55750903569647 7 28.000665097270478 33.11466802019795 20.844078446472796 18.040588436058773 8 26.18163488196253 34.59271472177882 19.753738553305887 19.471911842952757 9 26.03776213592913 17.140647818970262 20.677111264511378 36.14447878058923 10-11 24.797871688734478 26.87749083303433 27.243294331796026 21.081343146435167 12-13 26.134526262802705 24.272741370263322 27.824908347717543 21.767824019216437 14-15 22.777323683588723 25.459468447260413 26.616842713246815 25.14636515590405 16-17 21.927319545045304 28.973012443624235 26.87909297503014 22.22057503630032 18-19 21.844601821770617 27.621628594527824 29.19196538982394 21.341804193877618 20-21 22.75789631393915 26.355725403011398 29.323767687046608 21.562610596002845 22-23 22.37005662078079 26.341334920232086 29.373145822576554 21.915462636410574 24-25 22.57317397413082 26.525035046457013 29.21860184896863 21.683189130443534 26-27 22.289983098386436 26.62359462453072 29.407687363558328 21.67873491352451 28-29 21.821092905701676 27.05847097666111 29.352870686513494 21.76756543112372 30-31 22.185103436056526 26.474041934676812 29.263267476035566 22.0775871532311 32-33 21.986256893868987 26.672950129607365 29.31133946277267 22.029453513750983 34-35 22.61322523222659 26.48046531085194 29.086444128216034 21.81986532870543 36-37 22.421860640169452 26.34802321205066 29.221468521063272 22.008647626716616 38-39 22.08902278265293 26.83006280252344 29.22091368315734 21.86000073166629 40-41 22.346790185931805 26.650698130424455 29.099786605789248 21.90272507785449 42-43 22.100555377503195 26.828466960998597 28.654058925782387 22.41691873571582 44-45 22.37889618572869 26.68607174862776 28.576982048840595 22.35805001680296 46-47 22.741603634304656 27.046654887206863 28.18495677218297 22.02678470630551 48-49 22.788928175051097 27.210993062037698 27.81872768493575 22.181351077975457 50-51 22.24990815276169 27.366923897277417 28.131334462046482 22.251833487914407 52-53 22.846490982412902 27.513249831970427 27.43877422426347 22.2014849613532 54-55 22.476039018082382 27.209863353398035 27.68566118202785 22.628436446491733 56-57 22.707248859656293 27.432658855684934 27.51320621366333 22.346886070995442 58-59 23.175641001406515 27.11047564639224 27.46443276197517 22.249450590226072 60-61 22.78462623889703 27.17297380268906 28.11100615407611 21.9313938043378 62-63 22.497247722479415 27.292248991414997 28.021825692007457 22.188677594098134 64-65 22.380886783215427 27.545185734157577 27.74826532535882 22.325662157268184 66-67 22.570743166870106 27.453095469713908 27.355728116324148 22.620433247091846 68-69 22.341658915873822 27.354266133230094 27.74560970239406 22.55846524850203 70-71 22.668227252526982 27.212759697257543 27.641220680598167 22.477792369617305 72-73 22.637491015861446 26.997717074817 27.871274667652514 22.49351724166904 74-75 22.383187990381494 27.79294939362741 27.342672716331467 22.48118989965963 76-77 22.605272043028254 27.33897178655512 27.38036665173651 22.675389518680113 78-79 22.53791900122197 27.39340310640776 27.469581566353067 22.5990963260172 80-81 22.830520435660173 27.544419793035512 27.258007604520145 22.36705216678417 82-83 22.58818121892929 27.365224748817557 27.593293960034153 22.453300072218997 84-85 22.51184494023709 27.552267384976748 27.288554470972677 22.647333203813485 86-87 22.440994495444958 27.44625401464964 27.42763129107632 22.68512019882908 88-89 22.495448401978653 27.37910917149189 27.102320203564332 23.023122222965117 90-91 22.590272772493325 27.227401658485324 27.29256419025709 22.889761378764263 92-93 22.75315746730516 27.243201993895095 27.3037434540312 22.699897084768544 94-95 22.568317650668487 27.162885423636734 27.24513036935314 23.023666556341638 96-97 22.199599120108545 27.228712969717677 27.581058110081646 22.990629800092133 98-99 22.260056988434286 27.370953401103364 27.06180514716178 23.30718446330057 100-101 22.052650491046244 27.461309028421365 27.121004555420726 23.365035925111663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 602.0 1 448.5 2 346.5 3 408.0 4 368.5 5 348.5 6 592.5 7 1251.0 8 2145.5 9 2671.0 10 2562.0 11 2422.0 12 2609.5 13 2888.0 14 3197.5 15 3514.0 16 3724.0 17 3995.5 18 4296.0 19 4496.5 20 4813.5 21 5467.0 22 6574.0 23 7948.5 24 9572.5 25 11998.5 26 15477.0 27 19234.0 28 22588.5 29 26749.0 30 32568.5 31 38834.0 32 44468.5 33 49779.0 34 56177.5 35 61227.5 36 67127.5 37 74816.0 38 79518.0 39 81311.0 40 83256.5 41 87235.5 42 90162.5 43 93598.0 44 98999.0 45 101908.0 46 102287.0 47 111114.5 48 130383.5 49 129517.5 50 112358.5 51 103476.5 52 98686.0 53 95587.0 54 91639.0 55 85394.0 56 77628.5 57 69372.5 58 63856.0 59 59517.0 60 51857.5 61 41384.5 62 31740.5 63 24029.0 64 18016.0 65 13720.5 66 9792.5 67 7384.5 68 5280.0 69 3149.5 70 1975.0 71 1255.5 72 834.0 73 555.0 74 366.0 75 237.0 76 153.0 77 82.5 78 38.0 79 20.0 80 13.0 81 7.5 82 4.5 83 2.0 84 2.0 85 2.5 86 2.5 87 1.5 88 0.5 89 1.5 90 1.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02660389081903228 2 0.006615967585259344 3 0.00962640786214984 4 2.1003071699236014E-4 5 0.004830706490824284 6 0.0 7 0.0 8 1.7502559749363344E-4 9 3.500511949872669E-5 10-11 0.0 12-13 1.4002047799490676E-4 14-15 0.0 16-17 3.500511949872669E-5 18-19 0.003798055465611846 20-21 0.0015402252579439744 22-23 0.0 24-25 0.005583316560046907 26-27 0.006755988063254251 28-29 0.008681269635684219 30-31 0.030751997479631393 32-33 7.001023899745338E-4 34-35 0.004900716729821736 36-37 0.01575230377442701 38-39 0.008103685163955229 40-41 0.009608905302400477 42-43 0.006983521339995974 44-45 0.0029754351573917687 46-47 0.0020127943711767847 48-49 0.0017677585346856978 50-51 0.002782907000148772 52-53 0.0029754351573917687 54-55 0.001732753415186971 56-57 7.176049497238971E-4 58-59 8.051177484707138E-4 60-61 1.7502559749363345E-5 62-63 0.0 64-65 0.007333572534983242 66-67 6.300921509770804E-4 68-69 9.801433459643474E-4 70-71 0.003693040107115665 72-73 0.01057154608861546 74-75 0.00518075768581155 76-77 0.0031679633146347655 78-79 0.009608905302400477 80-81 1.0501535849618007E-4 82-83 0.00476069625182683 84-85 7.001023899745338E-4 86-87 0.0 88-89 0.0012601843019541608 90-91 7.701126289719872E-4 92-93 1.7502559749363344E-4 94-95 5.075742327315371E-4 96-97 3.150460754885402E-4 98-99 1.7502559749363344E-4 100-101 6.475947107264438E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2856725.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.21389216692295 #Duplication Level Percentage of deduplicated Percentage of total 1 72.35467867959777 34.88500675630486 2 14.814381764621439 14.285180098381755 3 5.21109012734773 7.537408124161807 4 2.4342633995238856 4.694612522021275 5 1.3578347925988992 3.273325013542975 6 0.821846516767428 2.3774651602311603 7 0.5541742012947949 1.8703226628042524 8 0.3906133119065419 1.506639047938132 9 0.29898545086021344 1.2973727058527853 >10 1.5570274920723235 13.720847579425236 >50 0.12705352496856537 4.227632104927758 >100 0.07223640824530507 6.285102047420087 >500 0.004070031136520448 1.321973219463172 >1k 0.0014535825487285194 1.4692523754972409 >5k 1.4535825487285194E-4 0.4987447604391288 >10k+ 1.4535825487285194E-4 0.7491158215883997 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10964 0.3837961301840394 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10414 0.36454331445973975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8616 0.3016041096010291 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5617 0.1966237562243478 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4914 0.17201515721674293 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4109 0.14383603602026795 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2971 0.10400021003071698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.500511949872669E-5 0.0 0.0 0.0 0.0 8 3.500511949872669E-5 0.0 0.0 0.0 0.0 9 3.500511949872669E-5 0.0 0.0 0.0 0.0 10-11 3.500511949872669E-5 0.0 0.0 0.0 0.0 12-13 7.001023899745338E-5 0.0 0.0 0.0 1.575230377442701E-4 14-15 1.4002047799490676E-4 0.0 0.0 0.0 1.925281572429968E-4 16-17 2.1003071699236014E-4 0.0 0.0 0.0 2.4503583649108685E-4 18-19 2.2753327674172348E-4 0.0 0.0 3.500511949872669E-5 3.500511949872669E-4 20-21 2.4503583649108685E-4 0.0 0.0 3.500511949872669E-5 3.850563144859936E-4 22-23 2.4503583649108685E-4 0.0 0.0 1.0501535849618006E-4 4.200614339847203E-4 24-25 3.150460754885402E-4 0.0 0.0 2.1003071699236014E-4 4.200614339847203E-4 26-27 3.150460754885402E-4 0.0 0.0 4.5506655348344696E-4 4.200614339847203E-4 28-29 3.150460754885402E-4 0.0 0.0 0.0012601843019541608 4.200614339847203E-4 30-31 3.500511949872669E-4 0.0 0.0 0.003097953075637312 4.200614339847203E-4 32-33 3.500511949872669E-4 0.0 0.0 0.004830706490824283 4.200614339847203E-4 34-35 3.6755375473663024E-4 0.0 0.0 0.006370931748768257 5.250767924809003E-4 36-37 3.8505631448599356E-4 0.0 0.0 0.008628761956436129 5.250767924809003E-4 38-39 4.025588742353569E-4 0.0 0.0 0.012164279025807525 5.950870314783537E-4 40-41 4.200614339847203E-4 0.0 0.0 0.016347390805905363 5.950870314783537E-4 42-43 4.200614339847203E-4 0.0 0.0 0.020495497466504475 5.950870314783537E-4 44-45 4.200614339847203E-4 0.0 0.0 0.02245578415843317 6.300921509770804E-4 46-47 4.7256911323281033E-4 0.0 0.0 0.025343706517078124 6.650972704758071E-4 48-49 4.900716729821737E-4 0.0 0.0 0.029334290139932967 7.176049497238971E-4 50-51 4.900716729821737E-4 0.0 0.0 0.03490010414023051 7.351075094732605E-4 52-53 4.900716729821737E-4 0.0 0.0 0.04634677821631414 7.351075094732605E-4 54-55 4.900716729821737E-4 0.0 0.0 0.054100412185282096 7.351075094732605E-4 56-57 5.250767924809003E-4 0.0 0.0 0.07484094548827766 7.701126289719872E-4 58-59 5.250767924809003E-4 0.0 0.0 0.11401167420735284 8.051177484707138E-4 60-61 5.425793522302637E-4 0.0 0.0 0.14539376383796132 8.051177484707138E-4 62-63 5.60081911979627E-4 0.0 0.0 0.16121607785138575 8.401228679694406E-4 64-65 5.60081911979627E-4 0.0 0.0 0.1938583517839484 8.751279874681672E-4 66-67 5.950870314783537E-4 0.0 0.0 0.2579877307056157 9.276356667162573E-4 68-69 6.300921509770804E-4 0.0 0.0 0.3529391173459119 9.451382264656206E-4 70-71 6.300921509770804E-4 0.0 0.0 0.4338709536269679 9.451382264656206E-4 72-73 6.475947107264438E-4 0.0 0.0 0.5233615416254627 9.801433459643472E-4 74-75 6.650972704758071E-4 0.0 0.0 0.6300221407380829 0.001015148465463074 76-77 6.650972704758071E-4 0.0 0.0 0.6989647235908252 0.001015148465463074 78-79 7.001023899745338E-4 0.0 0.0 0.7907131417969878 0.0010501535849618006 80-81 7.701126289719871E-4 0.0 0.0 0.9166790643131558 0.0010501535849618006 82-83 7.701126289719871E-4 0.0 0.0 1.0263150985831677 0.0010501535849618006 84-85 8.051177484707138E-4 0.0 0.0 1.1832605518557089 0.0010501535849618006 86-87 8.576254277188038E-4 0.0 0.0 1.4113364079496626 0.001067656144711164 88-89 8.751279874681672E-4 0.0 0.0 1.7025614996193195 0.0010851587044605274 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11965 0.0 22.00216 1 GAGTACT 10855 0.0 21.504911 12-13 TACATGG 12270 0.0 21.410892 2 ACATGGG 12125 0.0 20.528183 3 GTATCAA 24205 0.0 20.495745 1 GTACTTT 11510 0.0 19.993685 14-15 AGAGTAC 17505 0.0 19.900105 10-11 TAGGCCG 120 0.0047690766 19.788885 5 GGACCGT 265 1.8613719E-8 19.71352 6 AGTACTT 11280 0.0 19.642038 12-13 GGTACCG 175 9.9540805E-5 18.99733 5 GTACCGA 180 1.2357967E-4 18.468979 6 CAACGCA 27540 0.0 17.917778 5 TCAACGC 27525 0.0 17.91029 4 ATCAACG 27575 0.0 17.88063 3 TATCAAC 27875 0.0 17.758532 2 CATGGGG 5880 0.0 17.688839 4 AACGCAG 28040 0.0 17.665407 6 GGTATCA 19250 0.0 17.45247 1 CGGTTAC 400 0.0 17.225374 28-29 >>END_MODULE