FastQCFastQC Report
Thu 26 May 2016
SRR937105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953473
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97320.32951037642802217No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88520.29971494576046576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69960.23687367380707391No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54550.18469781169490967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.15605356812132698No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43950.1488078611180803No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32810.11108955456846906No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30400.10292966957883144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129800.025.1483381
TACATGG134200.023.5460722
GTATCAA212150.023.1134031
ACATGGG133400.022.868023
GAGTACT91000.021.56286412-13
TCAACGC240850.020.3404874
ATCAACG240600.020.3221243
AGAGTAC160750.020.27598610-11
CAACGCA242200.020.227115
AACGCAG246200.020.0136056
TATCAAC245150.019.925562
GTACTTT98100.019.75858714-15
CATGGGG62050.019.7572844
AGTACTT96000.019.40048812-13
GGTATCA162650.019.3680861
ACGCAGA265750.018.344627
CATGGGA77350.018.0607744
CGCAGAG268550.018.0468868
GCAGAGT274000.017.1324279
ACTTTTT119300.016.82555416-17
Produced by FastQC (version 0.11.5)