##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937105_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2953473 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.494161957803577 31.0 31.0 34.0 30.0 34.0 2 31.608330599263986 33.0 31.0 34.0 30.0 34.0 3 31.61486087734677 33.0 31.0 34.0 28.0 34.0 4 35.19547597015446 37.0 35.0 37.0 33.0 37.0 5 35.18001891332678 37.0 35.0 37.0 32.0 37.0 6 35.27218633791472 37.0 35.0 37.0 32.0 37.0 7 35.20091769926456 37.0 35.0 37.0 32.0 37.0 8 35.153675351018954 37.0 35.0 37.0 32.0 37.0 9 36.71224791965256 39.0 37.0 39.0 32.0 39.0 10-11 36.56748326461762 39.0 37.0 39.0 32.0 39.0 12-13 36.46271931383832 39.0 37.0 39.0 32.0 39.0 14-15 37.56188257011321 40.0 37.0 41.0 32.0 41.0 16-17 37.47985981249871 40.0 37.0 41.0 32.0 41.0 18-19 37.40858829588082 40.0 37.0 41.0 32.0 41.0 20-21 37.27905553902135 40.0 37.0 41.0 31.0 41.0 22-23 37.13766149208068 39.0 36.5 41.0 31.0 41.0 24-25 37.25941357852264 40.0 37.0 41.0 31.0 41.0 26-27 37.21109961052632 40.0 37.0 41.0 31.0 41.0 28-29 37.111728294113405 40.0 36.5 41.0 31.0 41.0 30-31 36.93896761541413 39.0 36.0 41.0 30.0 41.0 32-33 36.73566272655954 39.0 36.0 41.0 30.0 41.0 34-35 36.53384710136169 39.0 35.5 41.0 30.0 41.0 36-37 36.335356036774336 39.0 35.0 41.0 30.0 41.0 38-39 36.17179097286483 39.0 35.0 40.5 29.5 41.0 40-41 35.9888047055111 39.0 35.0 40.0 29.0 41.0 42-43 35.75814337899821 38.5 35.0 40.0 27.5 41.0 44-45 35.50198444339935 38.0 35.0 40.0 27.0 41.0 46-47 35.16400403863519 38.0 34.0 40.0 25.5 41.0 48-49 34.92066611071102 38.0 34.0 40.0 24.0 41.0 50-51 34.214290599575484 37.5 33.5 39.5 24.0 40.5 52-53 34.41022856819751 38.0 33.5 39.5 24.0 40.5 54-55 34.81076769620037 38.0 34.0 40.0 24.5 41.0 56-57 34.69643484128685 38.0 34.0 40.0 24.5 41.0 58-59 34.42865145542214 38.0 34.0 40.0 23.5 41.0 60-61 34.11750657615627 38.0 33.5 40.0 23.0 41.0 62-63 33.76986246361487 37.0 33.0 40.0 22.0 41.0 64-65 33.42568460927187 37.0 33.0 39.5 22.0 41.0 66-67 33.02834307271473 36.0 32.0 39.0 20.5 41.0 68-69 32.61323296336212 36.0 32.0 39.0 19.5 40.5 70-71 32.194281952128904 35.0 31.5 38.5 18.5 40.0 72-73 31.775376480502786 35.0 31.0 37.5 17.5 40.0 74-75 31.324115033386118 35.0 31.0 37.0 13.5 39.0 76-77 30.880640520499085 35.0 30.5 36.5 9.5 39.0 78-79 30.43663561508773 34.5 30.0 36.0 7.5 38.5 80-81 29.972870921792747 34.0 30.0 36.0 6.0 37.5 82-83 29.538043855488098 34.0 29.0 35.0 2.0 37.0 84-85 29.132619123316854 34.0 29.0 35.0 2.0 36.5 86-87 28.782256008434814 34.0 29.0 35.0 2.0 36.0 88-89 28.40701201602317 34.0 28.5 35.0 2.0 36.0 90-91 27.980870825634767 34.0 27.0 35.0 2.0 35.0 92-93 27.57114387028424 34.0 26.5 35.0 2.0 35.0 94-95 27.110732347984897 33.0 26.0 35.0 2.0 35.0 96-97 26.5382793409657 33.0 25.0 35.0 2.0 35.0 98-99 25.823339336435446 33.0 23.5 35.0 2.0 35.0 100-101 23.2006095874247 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4240197057235129 1101 2 0.3876599956773177 1101 3 0.3678140530096279 1101 4 0.38052362191034206 1101 5 0.5138084937052696 1101 6 0.301422978411118 1101 7 0.3447574990376623 1101 8 0.2499546775781667 1101 9 0.3830604478541133 1101 10-11 0.4496195040149189 1101 12-13 0.5734308121445508 1101 14-15 0.5903137695245917 1101 16-17 0.6636805208341627 1101 18-19 0.7225722431878339 1101 20-21 0.7559926116347668 1101 22-23 0.8187326939064405 1101 24-25 0.7875267462078881 1101 26-27 0.5926272778232047 1101 28-29 0.5241014630107514 1101 30-31 0.5507028701397658 1101 32-33 0.7575112379810705 1101 34-35 0.7872488037343501 1101 36-37 0.7005873105485065 1101 38-39 0.802137714675986 1101 40-41 0.6739241418889605 1101 42-43 0.5996808401679488 1101 44-45 0.6719410063188249 1101 46-47 0.6971710486331588 1101 48-49 0.7636201446932489 1101 50-51 0.8480814550587965 1101 52-53 0.7955297995687332 1101 54-55 0.7947585619593625 1101 56-57 0.7666307009616276 1101 58-59 0.745000641321667 1101 60-61 0.8659295291086906 1101 62-63 0.8628861198366664 1101 64-65 0.9300431595051037 1101 66-67 0.9327963552434397 1101 68-69 0.8996148432580995 1101 70-71 0.6961350736739433 1101 72-73 0.6950208546607151 1101 74-75 0.6253973968669442 1101 76-77 0.6421741459706567 1101 78-79 0.6323594430504187 1101 80-81 0.6239282600505227 1101 82-83 0.6176260683321821 1101 84-85 0.4886756094285154 1101 86-87 0.6283624291672147 1101 88-89 0.6927227096829149 1101 90-91 0.6313081672685286 1101 92-93 0.7966004555543122 1101 94-95 0.8197534827852735 1101 96-97 0.7706570075750712 1101 98-99 0.57945094703663 1101 100-101 1.0242260190625778 1102 1 0.18872403155253537 1102 2 0.3061479287478548 1102 3 0.20295296520931672 1102 4 0.15683672550252936 1102 5 0.1844528016618412 1102 6 0.16665096558286763 1102 7 0.26399427576657786 1102 8 0.2982668119451404 1102 9 0.38389188734254986 1102 10-11 0.3337662834201609 1102 12-13 0.31043264777544266 1102 14-15 0.2837785304011575 1102 16-17 0.563924509294182 1102 18-19 0.4211568114894888 1102 20-21 0.5183735753561152 1102 22-23 0.43784552018832557 1102 24-25 0.12397849739068079 1102 26-27 0.42670066601377954 1102 28-29 0.24612387388663137 1102 30-31 0.20118916490216776 1102 32-33 0.5374001550949217 1102 34-35 0.2911341153606699 1102 36-37 0.2364815304280512 1102 38-39 0.34050489528906525 1102 40-41 0.350011458344774 1102 42-43 0.5197107825738954 1102 44-45 0.1726509146431212 1102 46-47 0.4135388582597912 1102 48-49 0.33040218091293383 1102 50-51 0.3247890529679367 1102 52-53 0.3926694889630582 1102 54-55 0.6748755342873025 1102 56-57 0.5318958090052917 1102 58-59 0.3718678362992449 1102 60-61 0.3882205748890897 1102 62-63 0.5042987807000756 1102 64-65 0.5290601557234638 1102 66-67 0.4322769654470662 1102 68-69 0.37723694900193294 1102 70-71 0.2920964021928967 1102 72-73 0.20801731797153522 1102 74-75 0.3602294381520075 1102 76-77 0.4826359530540181 1102 78-79 0.37952585796045923 1102 80-81 0.3448690813668236 1102 82-83 0.4832588128725419 1102 84-85 0.43976744313910743 1102 86-87 0.39640971253933444 1102 88-89 0.4036966524940908 1102 90-91 0.38713712699200187 1102 92-93 0.311110955242075 1102 94-95 0.47346186544929836 1102 96-97 0.28165189230379895 1102 98-99 0.2710347154628714 1102 100-101 0.42417610245223614 1103 1 0.1394191744308344 1103 2 0.28641926415977537 1103 3 0.2331241395151622 1103 4 0.27000745265095105 1103 5 0.2717213779255445 1103 6 0.29196243013845447 1103 7 0.17568212250229465 1103 8 0.19891635842542854 1103 9 0.16459001726119737 1103 10-11 0.29086603978406345 1103 12-13 0.4280516746832532 1103 14-15 0.4266837883829524 1103 16-17 0.43655630930410894 1103 18-19 0.4202491969449369 1103 20-21 0.5078129409533148 1103 22-23 0.6047446020778011 1103 24-25 0.3534269589031567 1103 26-27 0.504752326714744 1103 28-29 0.5636283346773325 1103 30-31 0.5924577961619235 1103 32-33 0.6489520034294785 1103 34-35 0.4381721197876729 1103 36-37 0.6696131636525706 1103 38-39 0.6516962678826275 1103 40-41 0.5428197902822376 1103 42-43 0.5200714470268224 1103 44-45 0.568042856751326 1103 46-47 0.5364138720088008 1103 48-49 0.5044224424640831 1103 50-51 0.5056703412989236 1103 52-53 0.6486686413768794 1103 54-55 0.6118637205607129 1103 56-57 0.6355061199378582 1103 58-59 0.5667580203311218 1103 60-61 0.6628419109120784 1103 62-63 0.6894369160392486 1103 64-65 0.6324198841651949 1103 66-67 0.5033343777283719 1103 68-69 0.6005904193964469 1103 70-71 0.5133062847313994 1103 72-73 0.29989774433829197 1103 74-75 0.414695926147413 1103 76-77 0.40561061569099266 1103 78-79 0.33677038333735965 1103 80-81 0.3269402793245675 1103 82-83 0.2594536020507583 1103 84-85 0.21192963799190778 1103 86-87 0.4246837028585908 1103 88-89 0.39206211238687416 1103 90-91 0.30426490977414034 1103 92-93 0.34645719491414084 1103 94-95 0.5297145568409434 1103 96-97 0.30418715267954255 1103 98-99 0.42968825400935273 1103 100-101 0.6545501061430805 1104 1 0.37094504814065843 1104 2 0.34497570884783 1104 3 0.33811297734836643 1104 4 0.3392130589370197 1104 5 0.39435027822832325 1104 6 0.3492992824349628 1104 7 0.30084015564725064 1104 8 0.34964090080068644 1104 9 0.34590654009130617 1104 10-11 0.4774139675529341 1104 12-13 0.4941717612812795 1104 14-15 0.7123635581434655 1104 16-17 0.5737237855319961 1104 18-19 0.5453251148282945 1104 20-21 0.7203778068454909 1104 22-23 0.5882011742941273 1104 24-25 0.6220620879153529 1104 26-27 0.6365154739362211 1104 28-29 0.6425243165038665 1104 30-31 0.537076517959548 1104 32-33 0.6496054825343336 1104 34-35 0.5532703017523346 1104 36-37 0.5419310760725935 1104 38-39 0.6382172284487098 1104 40-41 0.673952655529888 1104 42-43 0.6663216544668416 1104 44-45 0.6965292693438485 1104 46-47 0.7326534234012456 1104 48-49 0.7341687217992003 1104 50-51 0.5172180366661081 1104 52-53 0.64760419862791 1104 54-55 0.5953962536977571 1104 56-57 0.7473979333389309 1104 58-59 0.9160064505007881 1104 60-61 0.754937964077314 1104 62-63 0.8006465444237918 1104 64-65 0.7602925841631958 1104 66-67 0.7870403255588414 1104 68-69 0.6374420531286376 1104 70-71 0.5876622135910097 1104 72-73 0.7663835278969238 1104 74-75 0.6628174319450615 1104 76-77 0.73875808506045 1104 78-79 0.8286384762732446 1104 80-81 0.8133938003650094 1104 82-83 0.7093443130071044 1104 84-85 0.8205503116193782 1104 86-87 0.6659427742709809 1104 88-89 0.629104341322158 1104 90-91 0.5577315682235451 1104 92-93 0.5361239102417024 1104 94-95 0.71639280170211 1104 96-97 0.7853440671977232 1104 98-99 0.7259280550184251 1104 100-101 0.738926765232879 1105 1 0.2567435061892489 1105 2 0.3176340777251312 1105 3 0.2691732492997012 1105 4 0.28727479824677715 1105 5 0.2823968198972864 1105 6 0.24816716709079145 1105 7 0.19934953796149557 1105 8 0.2091847197575163 1105 9 0.41694087804790314 1105 10-11 0.30868854971990345 1105 12-13 0.4340993087577516 1105 14-15 0.2880183566301042 1105 16-17 0.43045467124886727 1105 18-19 0.43192939494129945 1105 20-21 0.39766789155890336 1105 22-23 0.4331279407901718 1105 24-25 0.2029630245473868 1105 26-27 0.2579077357303916 1105 28-29 0.2835323733633217 1105 30-31 0.10854230931561659 1105 32-33 0.3277622598449952 1105 34-35 0.15399146655996532 1105 36-37 0.24131750450450085 1105 38-39 0.2838424279475191 1105 40-41 0.3042843782399842 1105 42-43 0.3599170024778431 1105 44-45 0.2696583448195682 1105 46-47 0.18252783698533648 1105 48-49 0.29639244359457706 1105 50-51 0.21055994887370133 1105 52-53 0.2474684968991383 1105 54-55 0.322846677605078 1105 56-57 0.5010761554531697 1105 58-59 0.44458046532545836 1105 60-61 0.42505259975250453 1105 62-63 0.4634984024765956 1105 64-65 0.361811063623243 1105 66-67 0.3133330353192889 1105 68-69 0.46149146043426015 1105 70-71 0.5318085441400342 1105 72-73 0.49680014607134737 1105 74-75 0.5229652771537197 1105 76-77 0.4422524875236533 1105 78-79 0.5450210123940131 1105 80-81 0.445152216067104 1105 82-83 0.31613415508912013 1105 84-85 0.4837872151391984 1105 86-87 0.3827024865026658 1105 88-89 0.24960255918949414 1105 90-91 0.19240177329300678 1105 92-93 0.11451996702221123 1105 94-95 0.4795395181473481 1105 96-97 0.28244503894835304 1105 98-99 0.08537374097785744 1105 100-101 0.20732985424014672 1106 1 0.07610446307143093 1106 2 0.20032857187972652 1106 3 0.2791318496657702 1106 4 0.23072325519268588 1106 5 0.32472539635149644 1106 6 0.22725270424083988 1106 7 0.3654727069709125 1106 8 0.34731062155065473 1106 9 0.3505581965548643 1106 10-11 0.380041609061081 1106 12-13 0.37951284623410686 1106 14-15 0.541337975060074 1106 16-17 0.3803032464794498 1106 18-19 0.21736279098590927 1106 20-21 0.4960353591963198 1106 22-23 0.3853540633473642 1106 24-25 0.4415652515621815 1106 26-27 0.30942007149569406 1106 28-29 0.48335864275665585 1106 30-31 0.4267859349446397 1106 32-33 0.32379437005367606 1106 34-35 0.28670686447060945 1106 36-37 0.30354926087887435 1106 38-39 0.2607246582253424 1106 40-41 0.30789868864546577 1106 42-43 0.2183701500226789 1106 44-45 0.43102106577482147 1106 46-47 0.4629583523903804 1106 48-49 0.2791096259722039 1106 50-51 0.3974538036792694 1106 52-53 0.21444668051569238 1106 54-55 0.03813458860606289 1106 56-57 0.19668012104864374 1106 58-59 0.18574671646624097 1106 60-61 0.22346378683091928 1106 62-63 0.28034236868389684 1106 64-65 0.22537212043557275 1106 66-67 0.22316187944355192 1106 68-69 0.20097674368015106 1106 70-71 0.2211912205345925 1106 72-73 0.1308976136577762 1106 74-75 0.36774813301467546 1106 76-77 0.21167500851663235 1106 78-79 0.13150150816992578 1106 80-81 0.20935313175358772 1106 82-83 0.34365841109385187 1106 84-85 0.2851736185205098 1106 86-87 0.295778805606151 1106 88-89 0.4124907444885437 1106 90-91 0.5146450267332057 1106 92-93 0.2910600894876296 1106 94-95 0.2661115315869296 1106 96-97 0.18316392421732175 1106 98-99 0.24204981433474515 1106 100-101 0.017734953736930237 1107 1 0.20769882683672947 1107 2 0.24797705747567278 1107 3 0.23936011511556998 1107 4 0.1962773718845341 1107 5 0.23736684179286982 1107 6 0.1768328643658208 1107 7 0.03223392753844223 1107 8 0.153678621221772 1107 9 0.24281505428207595 1107 10-11 0.16971236621171215 1107 12-13 0.2338661797729884 1107 14-15 0.2958297992309298 1107 16-17 0.362827218976598 1107 18-19 0.23361321493681686 1107 20-21 0.43402033334815115 1107 22-23 0.3012558197888495 1107 24-25 0.33783525433712924 1107 26-27 0.16729553784170292 1107 28-29 0.2934977500444944 1107 30-31 0.32086295526344344 1107 32-33 0.32202405381199384 1107 34-35 0.28893915873879195 1107 36-37 0.2749811358210579 1107 38-39 0.15345222993727248 1107 40-41 0.07691173091721737 1107 42-43 0.3222229645428598 1107 44-45 0.43664907839571043 1107 46-47 0.5201984062449938 1107 48-49 0.5076458904764678 1107 50-51 0.2892733496149944 1107 52-53 0.5400908289653117 1107 54-55 0.6392355762832267 1107 56-57 0.6351329828457821 1107 58-59 0.644915104630563 1107 60-61 0.6938866585973358 1107 62-63 0.8256817349672048 1107 64-65 0.7936007250460975 1107 66-67 0.7003332789440648 1107 68-69 0.6538028261681532 1107 70-71 0.6031617443837689 1107 72-73 0.5430182418245586 1107 74-75 0.6630571803841256 1107 76-77 0.5801646641143243 1107 78-79 0.48354597933612453 1107 80-81 0.5838711124111526 1107 82-83 0.39719518947800836 1107 84-85 0.39707173669232887 1107 86-87 0.2566663789625032 1107 88-89 0.3382165998416333 1107 90-91 0.31107895719497947 1107 92-93 0.18904105537628269 1107 94-95 0.2862466306115863 1107 96-97 0.19204531045972928 1107 98-99 0.129833090627411 1107 100-101 0.2244973562282162 1108 1 -0.08673328824115956 1108 2 0.10196557192241329 1108 3 -0.03425484090493924 1108 4 0.19918998161945467 1108 5 0.2624485363182032 1108 6 0.194730400915617 1108 7 0.019846719862023576 1108 8 0.2555003172009691 1108 9 0.13942318140141197 1108 10-11 0.020616800079992004 1108 12-13 0.11207676820448143 1108 14-15 0.1497235984860339 1108 16-17 0.17401691020079113 1108 18-19 0.30191779162618815 1108 20-21 0.37791353395193283 1108 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-1.7499706201927765 2316 64-65 -1.8579697775977841 2316 66-67 -1.8751699805218074 2316 68-69 -1.5199141168865289 2316 70-71 -1.6452314420210143 2316 72-73 -2.37072410929634 2316 74-75 -2.387563837680876 2316 76-77 -2.558761503697948 2316 78-79 -2.859325458536752 2316 80-81 -3.0831025653890336 2316 82-83 -2.611872408649763 2316 84-85 -2.5017476054050682 2316 86-87 -2.6754896803705 2316 88-89 -2.780252618767335 2316 90-91 -2.663737850095629 2316 92-93 -3.1603826132681654 2316 94-95 -2.6875507461201735 2316 96-97 -2.8404049323772576 2316 98-99 -2.6801561768825444 2316 100-101 -2.4229848044869833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7073.0 3 2862.0 4 2962.0 5 3252.0 6 3667.0 7 4429.0 8 5435.0 9 6719.0 10 8334.0 11 10150.0 12 12234.0 13 14283.0 14 15928.0 15 18435.0 16 21900.0 17 27404.0 18 25049.0 19 18604.0 20 19189.0 21 20782.0 22 22673.0 23 25651.0 24 29226.0 25 33272.0 26 38879.0 27 45549.0 28 54194.0 29 64511.0 30 77480.0 31 94412.0 32 118790.0 33 151041.0 34 199931.0 35 276996.0 36 400353.0 37 553290.0 38 450349.0 39 68173.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.132900239813516 18.698917706473814 14.325479195220932 22.84270285849174 2 15.0320301616509 20.425077730453612 40.23071604122237 24.31217606667312 3 19.54993058142291 24.716467440994208 29.722698181571904 26.01090379601097 4 12.634342053706682 16.585314972109682 37.80169919097594 32.978643783207694 5 13.82269712336698 37.43881315216143 34.001746042016805 14.736743682454783 6 31.383814734423225 36.25463041999637 17.773665612543255 14.58788923303715 7 28.11586625221682 32.81550807300124 21.011239113156797 18.057386561625147 8 26.092675523878565 34.18598112478913 20.130317545274085 19.591025806058223 9 26.17495488758474 17.172928215715917 20.73934913487122 35.91276776182812 10-11 24.68919141338575 26.553043875233595 27.588575790559545 21.16918892082111 12-13 26.04129126484559 24.059677712173723 27.868741684708535 22.030289338272155 14-15 22.84248565483834 25.210673396294915 26.598506829868278 25.348334118998466 16-17 22.07457125465319 28.745109758978103 26.79575237961393 22.384566606754777 18-19 21.941526107899286 27.523301916439554 28.96239251521177 21.57277946044939 20-21 22.900674305693713 26.288016999445485 28.943503087922185 21.867805606938617 22-23 22.47022333136374 26.361075227674057 28.97818832568353 22.19051311527867 24-25 22.58748469780676 26.456106004689822 28.85898416291776 22.097425134585656 26-27 22.340185100821188 26.581058933529576 29.0490360242176 22.029719941431633 28-29 21.92102230960953 27.000031489227126 28.994620082474686 22.08432611868865 30-31 22.22542748824858 26.478488206934685 28.887821219289968 22.408263085526766 32-33 22.03429226929933 26.63869838286528 28.967133344585655 22.35987600324974 34-35 22.61416011152973 26.514687372237255 28.721767343355996 22.14938517287701 36-37 22.446573914845334 26.302034926339264 28.87659714512372 22.374794013691677 38-39 22.16561646078162 26.766969073819475 28.895415678210213 22.17199878718869 40-41 22.42766777073428 26.579983392430435 28.78985668564715 22.202492151188135 42-43 22.14560995573854 26.73884723829323 28.35519883797801 22.76034396799022 44-45 22.420975184791047 26.59662139902508 28.33704817145657 22.645355244727305 46-47 22.730582390728202 26.924052958001134 28.044682996032638 22.30068165523803 48-49 22.63515744060447 27.012290318179033 27.759379413251494 22.593172827965002 50-51 22.200742787046156 27.151578600230035 28.094096742443043 22.553581870280766 52-53 22.794898512842174 27.335753664567147 27.396902438755998 22.472445383834682 54-55 22.41357681397906 26.976341814186444 27.675091652297716 22.934989719536773 56-57 22.649194055583273 27.18299432140704 27.494493324017704 22.67331829899198 58-59 23.035241592690834 26.93914422561879 27.411355970423664 22.61425821126672 60-61 22.741509917898945 26.90082270353638 28.08177769651475 22.275889682049925 62-63 22.421510087509162 27.071685055046636 28.028225048714955 22.478579808729247 64-65 22.317343278381962 27.33652521585499 27.72387151953348 22.62225998622957 66-67 22.463550096109937 27.331286125883107 27.264255517496107 22.940908260510845 68-69 22.19467243473881 27.185821378834508 27.8102292977993 22.809276888627387 70-71 22.560076568000582 27.037973603208894 27.638430240837998 22.76351958795253 72-73 22.533962578514547 26.826576488102095 27.829808503750144 22.809652429633225 74-75 22.223979423809084 27.60602825501134 27.430050348340757 22.739941972838817 76-77 22.46049554362975 27.119218699989673 27.402858241194373 23.017427515186206 78-79 22.41921536242834 27.210610435656335 27.53869841389714 22.831475788018185 80-81 22.54799921478388 27.400147050180497 27.374961192268366 22.676892542767263 82-83 22.47140033769194 27.20274556758157 27.645962007116253 22.679892087610227 84-85 22.421296567530984 27.34442439251865 27.30219456268082 22.932084477269548 86-87 22.403709121213716 27.24615223373708 27.54658793661931 22.803550708429892 88-89 22.417477181312144 27.2149551888853 27.161146633562044 23.206420996240514 90-91 22.501289474602178 27.023933258216992 27.451093945209777 23.023683321971056 92-93 22.736957903767557 27.012288242799233 27.42058671016431 22.830167143268902 94-95 22.51394178322421 26.86723893855355 27.41261803128831 23.206201246933926 96-97 22.118303681414258 26.9896835331905 27.759519834820033 23.132492950575205 98-99 22.26328403714089 27.086279541603158 27.26993158051334 23.380504840742606 100-101 22.30171866434009 26.85884915082952 27.325931442095193 23.513500742735193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 413.0 1 308.5 2 263.5 3 351.0 4 321.0 5 328.0 6 579.5 7 1176.5 8 2045.0 9 2545.0 10 2346.0 11 2192.0 12 2402.5 13 2597.0 14 2844.0 15 3078.0 16 3233.0 17 3387.0 18 3585.0 19 3825.5 20 4241.0 21 4973.5 22 6017.5 23 7403.5 24 9158.5 25 11642.5 26 15188.5 27 19012.5 28 22462.5 29 26719.5 30 32603.0 31 39044.0 32 44901.0 33 50828.0 34 57619.5 35 63284.0 36 69578.0 37 76913.0 38 81858.5 39 84205.0 40 86165.5 41 90245.0 42 93547.0 43 96968.0 44 102071.0 45 105609.5 46 107357.5 47 115442.5 48 132394.0 49 132664.0 50 117462.5 51 108433.5 52 103133.0 53 99924.0 54 96306.5 55 89089.5 56 81048.0 57 73166.5 58 67358.0 59 62923.5 60 55237.5 61 44105.5 62 33545.5 63 25613.5 64 19548.5 65 14889.0 66 10578.5 67 7837.5 68 5615.0 69 3486.5 70 2230.5 71 1400.0 72 906.0 73 600.5 74 421.0 75 290.5 76 194.5 77 108.0 78 60.5 79 44.5 80 35.5 81 26.0 82 23.0 83 18.5 84 13.0 85 11.5 86 10.0 87 9.0 88 7.5 89 5.5 90 6.0 91 5.0 92 4.0 93 4.0 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025800134282588667 2 0.01080084361698922 3 0.012629199589771092 4 0.02221113922490573 5 0.05600186627743 6 0.04256006403308918 7 0.016319769979275246 8 0.049196319045408575 9 0.02874581890540391 10-11 0.02339618476281991 12-13 0.03660097789957789 14-15 0.018994587050567245 16-17 0.017792612290682866 18-19 0.01682764663838132 20-21 0.01474535233604641 22-23 0.008058309657816409 24-25 0.0019130020826328867 26-27 5.0787665910607614E-5 28-29 0.0027763924031132163 30-31 0.0 32-33 2.877967734934431E-4 34-35 1.6929221970202537E-5 36-37 0.0 38-39 1.5236299773182285E-4 40-41 1.6929221970202537E-5 42-43 1.6929221970202537E-5 44-45 6.771688788081015E-5 46-47 1.6929221970202537E-5 48-49 3.3858443940405075E-4 50-51 6.771688788081015E-5 52-53 6.940981007783041E-4 54-55 0.0018791436386924815 56-57 4.2323054925506344E-4 58-59 0.0010496117621525574 60-61 0.00340277361601071 62-63 0.002302374187947545 64-65 0.0014728423114076209 66-67 0.01166423393746955 68-69 0.01474535233604641 70-71 0.02720525970611548 72-73 0.017504815517189423 74-75 0.018469781169490967 76-77 0.03243638929490806 78-79 0.02053514624985568 80-81 0.048062061173405 82-83 0.036499402567756675 84-85 0.019773331261196566 86-87 0.014508343228463574 88-89 0.013915820459506486 90-91 0.024378079637091654 92-93 0.033045841285835355 94-95 0.013187863914787776 96-97 0.019383959155881907 98-99 0.029744643001645858 100-101 0.04095178794591994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2953473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.65175036426093 #Duplication Level Percentage of deduplicated Percentage of total 1 74.52743089229892 37.74944824846434 2 13.630516995335737 13.808190883671234 3 4.846636671034972 7.3647189230260794 4 2.305602939476469 4.671312981178733 5 1.1901609916577407 3.0141868721364555 6 0.7608821732960421 2.3124008339044466 7 0.5123102961395919 1.8164589260372237 8 0.35703603174537224 1.446759996081035 9 0.26899532405232934 1.2262575602746855 >10 1.4161724638049769 13.110284497119885 >50 0.11496834761688787 4.0485756774127415 >100 0.06426865104176348 5.87682528999713 >500 0.003345481666224905 1.0934221209123276 >1k 0.0014051022996694842 1.4093530947005202 >5k 2.6763853327037794E-4 1.0518040950830985 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9732 0.32951037642802217 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8852 0.29971494576046576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6996 0.23687367380707391 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5455 0.18469781169490967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4609 0.15605356812132698 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4395 0.1488078611180803 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3281 0.11108955456846906 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3040 0.10292966957883144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.3858443940405074E-5 7 0.0 0.0 0.0 0.0 3.3858443940405074E-5 8 0.0 0.0 0.0 0.0 3.3858443940405074E-5 9 0.0 0.0 0.0 0.0 3.3858443940405074E-5 10-11 0.0 0.0 0.0 0.0 6.771688788081015E-5 12-13 0.0 0.0 0.0 0.0 1.6929221970202538E-4 14-15 3.3858443940405074E-5 0.0 0.0 0.0 1.6929221970202538E-4 16-17 3.3858443940405074E-5 0.0 0.0 1.6929221970202537E-5 1.6929221970202538E-4 18-19 8.464610985101269E-5 0.0 0.0 5.078766591060761E-5 2.3700910758283554E-4 20-21 1.6929221970202538E-4 0.0 0.0 8.464610985101269E-5 2.708675515232406E-4 22-23 1.6929221970202538E-4 0.0 0.0 1.862214416722279E-4 3.047259954636457E-4 24-25 1.6929221970202538E-4 0.0 0.0 3.724428833444558E-4 3.047259954636457E-4 26-27 1.6929221970202538E-4 0.0 0.0 5.925227689570888E-4 3.216552174338482E-4 28-29 2.0315066364243046E-4 0.0 0.0 0.0014389838674672157 3.724428833444558E-4 30-31 2.0315066364243046E-4 0.0 0.0 0.0028948969569046338 4.063013272848609E-4 32-33 2.20079885612633E-4 0.0 0.0 0.004046084050878407 4.4015977122526597E-4 34-35 2.7086755152324065E-4 0.0 0.0 0.005366563364554204 4.4015977122526597E-4 36-37 3.047259954636457E-4 0.0 0.0 0.0074996453327997245 4.4015977122526597E-4 38-39 3.3858443940405075E-4 0.0 0.0 0.011054781946542257 4.4015977122526597E-4 40-41 3.3858443940405075E-4 3.3858443940405074E-5 0.0 0.015117795219390866 4.4015977122526597E-4 42-43 3.3858443940405075E-4 3.3858443940405074E-5 0.0 0.018960728606626842 4.570889931954685E-4 44-45 3.724428833444558E-4 3.3858443940405074E-5 0.0 0.02080601380137892 4.909474371358735E-4 46-47 4.0630132728486086E-4 3.3858443940405074E-5 0.0 0.023667052314343146 5.078766591060761E-4 48-49 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.027509985701579122 6.094519909272914E-4 50-51 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.032910407510073736 6.433104348676965E-4 52-53 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.04447306611572206 6.433104348676965E-4 54-55 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.05234515433186625 6.433104348676965E-4 56-57 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.0727279375839901 6.771688788081015E-4 58-59 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.11159743122757512 7.448857666889116E-4 60-61 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.14239168599137353 7.448857666889116E-4 62-63 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.15757719809864523 7.448857666889116E-4 64-65 4.4015977122526597E-4 3.3858443940405074E-5 0.0 0.18957342762232804 7.448857666889116E-4 66-67 4.740182151656711E-4 3.3858443940405074E-5 0.0 0.25302415156664715 7.448857666889116E-4 68-69 4.740182151656711E-4 3.3858443940405074E-5 0.0 0.34581321718532726 7.448857666889116E-4 70-71 4.740182151656711E-4 3.3858443940405074E-5 0.0 0.42543134811118977 7.787442106293168E-4 72-73 4.909474371358735E-4 3.3858443940405074E-5 0.0 0.5157487473222203 8.295318765399243E-4 74-75 5.078766591060761E-4 3.3858443940405074E-5 0.0 0.6196772409972937 8.464610985101269E-4 76-77 5.078766591060761E-4 3.3858443940405074E-5 0.0 0.6927437630206879 8.464610985101269E-4 78-79 5.078766591060761E-4 3.3858443940405074E-5 0.0 0.780437132826337 8.464610985101269E-4 80-81 5.417351030464812E-4 3.3858443940405074E-5 0.0 0.9047484097535342 8.464610985101269E-4 82-83 5.755935469868862E-4 3.3858443940405074E-5 0.0 1.0166167085326325 9.141779863909371E-4 84-85 5.755935469868862E-4 3.3858443940405074E-5 0.0 1.170689557683446 9.141779863909371E-4 86-87 5.755935469868862E-4 3.3858443940405074E-5 0.0 1.394121429246179 9.9882409624195E-4 88-89 5.925227689570888E-4 3.3858443940405074E-5 0.0 1.6713882266741562 0.0010665409841227598 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12980 0.0 25.148338 1 TACATGG 13420 0.0 23.546072 2 GTATCAA 21215 0.0 23.113403 1 ACATGGG 13340 0.0 22.86802 3 GAGTACT 9100 0.0 21.562864 12-13 TCAACGC 24085 0.0 20.340487 4 ATCAACG 24060 0.0 20.322124 3 AGAGTAC 16075 0.0 20.275986 10-11 CAACGCA 24220 0.0 20.22711 5 AACGCAG 24620 0.0 20.013605 6 TATCAAC 24515 0.0 19.92556 2 GTACTTT 9810 0.0 19.758587 14-15 CATGGGG 6205 0.0 19.757284 4 AGTACTT 9600 0.0 19.400488 12-13 GGTATCA 16265 0.0 19.368086 1 ACGCAGA 26575 0.0 18.34462 7 CATGGGA 7735 0.0 18.060774 4 CGCAGAG 26855 0.0 18.046886 8 GCAGAGT 27400 0.0 17.132427 9 ACTTTTT 11930 0.0 16.825554 16-17 >>END_MODULE