FastQCFastQC Report
Thu 26 May 2016
SRR937105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953473
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111960.3790791383567752No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105280.35646169780458464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86660.2934172751875504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.19390730844669987No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49570.16783630661258794No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41110.13919206303900528No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29590.10018713561965863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124500.022.5509011
TACATGG126750.021.434482
GAGTACT112100.021.20514912-13
ACATGGG125600.020.6083093
AGTACTT116550.020.55851412-13
AGAGTAC178400.020.5311210-11
GTATCAA250600.020.0373231
GTACTTT120900.019.38734814-15
CATGGGG59800.017.710844
CAACGCA286500.017.4273455
TCAACGC286550.017.4030114
ATCAACG287050.017.3561523
AACGCAG290400.017.2260196
TATCAAC290200.017.2016532
TACTTTT138750.017.0985814-15
GGTATCA200050.016.7178291
ACTTTTT142400.016.57692316-17
AGTACAT97600.015.9620592
GAGTACA105150.015.6771091
ACGCAGA318800.015.6318477
Produced by FastQC (version 0.11.5)