##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937105_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2953473 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11601934400619 31.0 31.0 34.0 30.0 34.0 2 32.247813675628656 33.0 31.0 34.0 30.0 34.0 3 32.35684666831219 34.0 31.0 34.0 30.0 34.0 4 36.009498309278605 37.0 35.0 37.0 35.0 37.0 5 35.90898579401268 37.0 35.0 37.0 35.0 37.0 6 35.94599205748622 37.0 35.0 37.0 35.0 37.0 7 35.88747620174622 37.0 35.0 37.0 35.0 37.0 8 35.85385612125115 37.0 35.0 37.0 35.0 37.0 9 37.52304998217353 39.0 37.0 39.0 35.0 39.0 10-11 37.435977406937525 39.0 37.0 39.0 34.0 39.0 12-13 37.33633217571314 39.0 37.0 39.0 34.0 39.0 14-15 38.52546967586973 40.0 38.0 41.0 34.0 41.0 16-17 38.44600830953931 40.0 38.0 41.0 34.0 41.0 18-19 38.59110968679924 40.0 38.0 41.0 34.0 41.0 20-21 38.61456190728678 40.0 38.0 41.0 34.0 41.0 22-23 38.536819872739656 40.0 38.0 41.0 34.0 41.0 24-25 38.425777042823825 40.0 38.0 41.0 34.0 41.0 26-27 38.24035482972081 40.0 38.0 41.0 34.0 41.0 28-29 38.16603910040823 40.0 38.0 41.0 33.5 41.0 30-31 38.1747011061215 40.0 38.0 41.0 34.0 41.0 32-33 38.02535743512807 40.0 37.0 41.0 33.0 41.0 34-35 37.872936370164894 40.0 37.0 41.0 33.0 41.0 36-37 37.720846102199005 40.0 37.0 41.0 33.0 41.0 38-39 37.58290781733911 40.0 37.0 41.0 33.0 41.0 40-41 37.41837253971849 40.0 37.0 41.0 32.0 41.0 42-43 37.17661004519087 40.0 36.0 41.0 31.5 41.0 44-45 36.92845693866171 39.0 36.0 41.0 31.0 41.0 46-47 36.598679588403215 39.0 36.0 41.0 30.5 41.0 48-49 36.25835990374722 39.0 35.0 40.5 30.0 41.0 50-51 35.96846221380727 39.0 35.0 40.0 29.0 41.0 52-53 35.73098670615916 39.0 35.0 40.0 28.5 41.0 54-55 35.443335862559096 38.0 35.0 40.0 28.0 41.0 56-57 35.139744971428556 38.0 34.0 40.0 27.0 41.0 58-59 34.77697646127119 38.0 34.0 40.0 26.0 41.0 60-61 34.41455483087199 37.5 34.0 40.0 26.0 41.0 62-63 34.044920336160175 37.0 33.0 39.5 26.0 41.0 64-65 33.66136392646894 36.0 33.0 39.0 25.0 40.0 66-67 33.24462996614494 36.0 32.0 39.0 24.0 40.0 68-69 32.926365333287286 35.5 32.0 38.5 23.0 40.0 70-71 32.4501825478005 35.0 31.5 38.0 22.0 40.0 72-73 31.928853759624687 35.0 31.0 37.0 21.0 39.0 74-75 31.415359646084458 34.5 31.0 36.5 20.0 39.0 76-77 29.99494019413755 33.5 29.0 35.0 18.5 37.5 78-79 30.753140286029364 34.0 30.0 36.0 19.5 38.0 80-81 30.51928255311628 34.0 30.0 35.5 18.0 37.0 82-83 30.19083330032135 34.0 30.0 35.0 15.5 37.0 84-85 29.82418901408613 34.0 30.0 35.0 12.0 36.0 86-87 29.47748379619519 34.0 29.0 35.0 7.0 36.0 88-89 29.07520028116052 34.0 29.0 35.0 4.5 35.5 90-91 28.592364480731668 33.5 29.0 35.0 2.0 35.0 92-93 28.085268089466197 33.0 28.0 35.0 2.0 35.0 94-95 27.468127692381138 33.0 26.5 34.5 2.0 35.0 96-97 26.76054411196581 32.5 25.5 34.0 2.0 35.0 98-99 25.900422485663487 32.0 24.5 34.0 2.0 35.0 100-101 22.35563504389578 28.0 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18440735295414612 1101 2 0.13416197021101084 1101 3 0.1567988909421274 1101 4 0.17819651706381023 1101 5 0.19568838946760536 1101 6 0.18155959285849832 1101 7 0.21235254846354934 1101 8 0.2614746185347059 1101 9 0.28518178506889313 1101 10-11 0.3165574926856678 1101 12-13 0.3109955056042253 1101 14-15 0.38712833577331196 1101 16-17 0.34476929395314215 1101 18-19 0.23015756855761538 1101 20-21 0.2532297389406608 1101 22-23 0.29728145913253456 1101 24-25 0.20464882192676725 1101 26-27 0.137830188677178 1101 28-29 0.22035534315905636 1101 30-31 0.27833312394385246 1101 32-33 0.3395750507297848 1101 34-35 0.3758610590758593 1101 36-37 0.2918227125142394 1101 38-39 0.22175866483398465 1101 40-41 0.344960640933472 1101 42-43 0.3640367864944949 1101 44-45 0.3982671160605875 1101 46-47 0.29048838872108007 1101 48-49 0.39844591839491983 1101 50-51 0.3835444730254096 1101 52-53 0.3570569709650613 1101 54-55 0.26172517659420436 1101 56-57 0.3319309560139274 1101 58-59 0.3634340378045451 1101 60-61 0.43936876099559186 1101 62-63 0.3762057352181003 1101 64-65 0.3617799867651499 1101 66-67 0.15919562759943062 1101 68-69 0.2222062928581252 1101 70-71 0.19894161435811242 1101 72-73 0.4306419081301236 1101 74-75 0.5031897219248371 1101 76-77 0.43477434237905044 1101 78-79 0.33957877472542464 1101 80-81 0.5399524662546007 1101 82-83 0.5684465218625192 1101 84-85 0.4509893058517669 1101 86-87 0.1382218359100449 1101 88-89 0.18692920590997986 1101 90-91 0.49425823801598767 1101 92-93 0.5663520515261773 1101 94-95 0.560837266562249 1101 96-97 0.6404256046633883 1101 98-99 0.7470065093441427 1101 100-101 1.049893221267972 1102 1 0.23159603282707053 1102 2 0.23025872187011487 1102 3 0.19901802353848552 1102 4 0.15230376387451372 1102 5 0.2034238575156806 1102 6 0.10491463169555004 1102 7 0.11469101218867195 1102 8 0.18020183380400567 1102 9 0.24348827948261942 1102 10-11 0.20084290834115137 1102 12-13 0.27867870580139 1102 14-15 0.38579281601305837 1102 16-17 0.35161728209260446 1102 18-19 0.3282293433889052 1102 20-21 0.2642247181726347 1102 22-23 0.22255047204999556 1102 24-25 0.37633928072864364 1102 26-27 0.2935490332276629 1102 28-29 0.39344624544573037 1102 30-31 0.45762003653062067 1102 32-33 0.340335234776731 1102 34-35 0.3761790624719268 1102 36-37 0.36059319136592194 1102 38-39 0.4490152320530001 1102 40-41 0.3848658617459648 1102 42-43 0.4747804060138776 1102 44-45 0.398089733482756 1102 46-47 0.35297014959613904 1102 48-49 0.3449906343030662 1102 50-51 0.35062427004757524 1102 52-53 0.3687683792447558 1102 54-55 0.39711164947630806 1102 56-57 0.30894361037551477 1102 58-59 0.42566583761493604 1102 60-61 0.44015909157548094 1102 62-63 0.35285993879774225 1102 64-65 0.3616323727072981 1102 66-67 0.34452384868559705 1102 68-69 0.29025949213293956 1102 70-71 0.2728950287978975 1102 72-73 0.3206291766538989 1102 74-75 0.2855156885417607 1102 76-77 0.18855721249531143 1102 78-79 0.07110925088565878 1102 80-81 0.49318007314532153 1102 82-83 0.28546073363747126 1102 84-85 0.2355337359821199 1102 86-87 0.2500757739733821 1102 88-89 0.3062476712378732 1102 90-91 0.4819726280618468 1102 92-93 0.4119434943713749 1102 94-95 0.286437664534283 1102 96-97 0.39157599258027886 1102 98-99 0.4800566920756033 1102 100-101 0.6187980837241795 1103 1 0.22157929317980773 1103 2 0.23070920421781693 1103 3 0.1712416036878608 1103 4 0.1521637575913033 1103 5 0.18062970233767572 1103 6 0.16067837944987673 1103 7 0.04869629180943491 1103 8 0.029999354884026275 1103 9 0.14090276421995895 1103 10-11 0.16687453538310848 1103 12-13 0.2923154346076089 1103 14-15 0.33645237321218957 1103 16-17 0.3015885349745062 1103 18-19 0.2275056510907234 1103 20-21 0.29588298745773045 1103 22-23 0.31535814798882456 1103 24-25 0.13144115282293 1103 26-27 0.2842075228564127 1103 28-29 0.25214957698420903 1103 30-31 0.28652090093911653 1103 32-33 0.30653045709376414 1103 34-35 0.38957037589981525 1103 36-37 0.25661898091868096 1103 38-39 0.20756347832603694 1103 40-41 0.4253059757207822 1103 42-43 0.4376488166424153 1103 44-45 0.4376098318430053 1103 46-47 0.3295677129476289 1103 48-49 0.35829325312148796 1103 50-51 0.38252009171371526 1103 52-53 0.3412132915350412 1103 54-55 0.4770636842942011 1103 56-57 0.41574270369554256 1103 58-59 0.3216696107752881 1103 60-61 0.32453299749557374 1103 62-63 0.3978386646722569 1103 64-65 0.2883522034201107 1103 66-67 0.2837200544613978 1103 68-69 0.22969558672327395 1103 70-71 0.09994534802660127 1103 72-73 0.35652596093063593 1103 74-75 0.5356699830143334 1103 76-77 0.3345217156006086 1103 78-79 0.5684263962096665 1103 80-81 0.6092659953068633 1103 82-83 0.6008075430398527 1103 84-85 0.5493071820921038 1103 86-87 0.40189564897059427 1103 88-89 0.3947935577130899 1103 90-91 0.573862130574053 1103 92-93 0.523608884386821 1103 94-95 0.20512510652783078 1103 96-97 0.334525633811932 1103 98-99 0.38096643636913186 1103 100-101 0.6852579790232056 1104 1 0.16691265015499113 1104 2 0.22286220440305016 1104 3 0.19973663235762018 1104 4 0.07509626056776852 1104 5 0.10620624787773636 1104 6 0.0375593698184602 1104 7 0.12248262135709354 1104 8 0.11340771666218075 1104 9 0.10728327842331709 1104 10-11 0.21580485929251836 1104 12-13 0.1136595667577538 1104 14-15 0.053633425141399016 1104 16-17 0.1723916618272625 1104 18-19 0.16528586726904848 1104 20-21 0.24202514532974106 1104 22-23 0.23406038083534497 1104 24-25 0.21312561055577817 1104 26-27 0.06189646198232168 1104 28-29 0.0749661889877018 1104 30-31 0.24969909208967778 1104 32-33 0.1842124585990419 1104 34-35 0.15691748313579978 1104 36-37 0.21058037951546282 1104 38-39 0.3041973034787091 1104 40-41 0.3292198438694882 1104 42-43 0.263274648554372 1104 44-45 0.26373278817145973 1104 46-47 0.3120212567117946 1104 48-49 0.15298465639384773 1104 50-51 0.25338984038248213 1104 52-53 0.25663319489724756 1104 54-55 0.17530666731270372 1104 56-57 0.2209666373505499 1104 58-59 0.19318479056467197 1104 60-61 0.2362198412190395 1104 62-63 0.29312522283431264 1104 64-65 0.15304936336824682 1104 66-67 0.13912519256997768 1104 68-69 0.23706633523573117 1104 70-71 0.10167144697670238 1104 72-73 0.1640171881974446 1104 74-75 0.18127768587345727 1104 76-77 0.2458315521482035 1104 78-79 0.31889688050722853 1104 80-81 0.5224254479957331 1104 82-83 0.4170523949121865 1104 84-85 0.3861885718472884 1104 86-87 0.2865136787276512 1104 88-89 0.2812656364583539 1104 90-91 0.26936354839647336 1104 92-93 0.27351252376276136 1104 94-95 0.23656556831514308 1104 96-97 0.3096914481095574 1104 98-99 0.15831641333554458 1104 100-101 0.39164504867553873 1105 1 0.20540860437014175 1105 2 0.1998613101039055 1105 3 0.1577486407398041 1105 4 0.09490321718064365 1105 5 0.14160575934810993 1105 6 0.1274907116006787 1105 7 0.17738299275666947 1105 8 0.07973138149192494 1105 9 0.24486931388084798 1105 10-11 0.13458429919173653 1105 12-13 0.15288817929413057 1105 14-15 0.22554699562054026 1105 16-17 0.19002289035881148 1105 18-19 0.17036700415874861 1105 20-21 0.2364693373876392 1105 22-23 0.2914110995755337 1105 24-25 0.15597596449815399 1105 26-27 0.20818149635567096 1105 28-29 0.18757846130383626 1105 30-31 0.19175519404794983 1105 32-33 0.21806719419888765 1105 34-35 0.26446737740568693 1105 36-37 0.203277617056699 1105 38-39 0.3825425710095942 1105 40-41 0.2221342416093819 1105 42-43 0.31977637011425486 1105 44-45 0.3496303830920553 1105 46-47 0.3332478877017806 1105 48-49 0.3107612051341633 1105 50-51 0.39580387468572553 1105 52-53 0.37706479972864315 1105 54-55 0.35385185051811874 1105 56-57 0.34891713544249825 1105 58-59 0.32823425606098766 1105 60-61 0.39297996691094994 1105 62-63 0.4542070712839532 1105 64-65 0.22194166259502168 1105 66-67 0.16723372434719863 1105 68-69 0.21985618106100446 1105 70-71 0.16350931510091726 1105 72-73 0.23501285996651333 1105 74-75 0.45538007734100105 1105 76-77 0.28267392069337305 1105 78-79 0.49919820623425437 1105 80-81 0.4939237036359785 1105 82-83 0.4908917542155855 1105 84-85 0.5875513571603612 1105 86-87 0.334700213264 1105 88-89 0.3351218506464022 1105 90-91 0.35620861379124946 1105 92-93 0.42619211182706707 1105 94-95 0.2688861197723931 1105 96-97 0.4967683319738576 1105 98-99 0.2788081655697603 1105 100-101 0.619399782710019 1106 1 0.17771801055052094 1106 2 0.20362730020266184 1106 3 0.08916443684150721 1106 4 0.08846027168846149 1106 5 0.11379968502250648 1106 6 0.1320250421961262 1106 7 0.1230633080464898 1106 8 0.1307115637115004 1106 9 0.13199361985065394 1106 10-11 0.0907882535705653 1106 12-13 0.18375831088582828 1106 14-15 0.32841960507209933 1106 16-17 0.16555746568116092 1106 18-19 -0.017597913220427586 1106 20-21 0.1971775413471093 1106 22-23 0.09091272344048917 1106 24-25 0.13429978917172036 1106 26-27 0.14121771593688237 1106 28-29 0.15787256695448093 1106 30-31 0.17070447439790826 1106 32-33 0.034855459544026246 1106 34-35 0.04227791289873295 1106 36-37 0.15615914884315174 1106 38-39 0.21591478023070465 1106 40-41 0.27984058972320014 1106 42-43 0.06177979119238586 1106 44-45 -0.08630470309770288 1106 46-47 -0.053772154411639406 1106 48-49 -0.05331199370134243 1106 50-51 -0.09915768279704906 1106 52-53 -0.07005545295187687 1106 54-55 -0.14657642155701467 1106 56-57 0.05040652806092538 1106 58-59 0.2156329875598857 1106 60-61 0.09280050051755495 1106 62-63 0.3123548200514037 1106 64-65 -0.1158426412334208 1106 66-67 0.07482561165046775 1106 68-69 -0.06644688771235252 1106 70-71 -0.07363105107170043 1106 72-73 -0.11674088868875643 1106 74-75 -0.09319897709544023 1106 76-77 -0.04140121556342535 1106 78-79 0.07893392667455856 1106 80-81 0.14929915548851014 1106 82-83 0.19676379773618535 1106 84-85 0.1915284242104356 1106 86-87 -0.04620866675353952 1106 88-89 -0.02538024737691913 1106 90-91 7.300452396741264E-4 1106 92-93 0.13295090797465647 1106 94-95 0.0917744580257569 1106 96-97 0.09912918178801888 1106 98-99 -7.66060608878405E-4 1106 100-101 0.09100547159074779 1107 1 0.1032110783107214 1107 2 0.12851296192580008 1107 3 0.10895703491548403 1107 4 0.08480555786817945 1107 5 0.05875903712273356 1107 6 0.07474850286889989 1107 7 0.0946580829157071 1107 8 0.13974351676343133 1107 9 0.0812860072338708 1107 10-11 0.11278154229912474 1107 12-13 0.014620652739289142 1107 14-15 0.17947280191893356 1107 16-17 0.13348904771181225 1107 18-19 0.060564503659506386 1107 20-21 0.018296206492685485 1107 22-23 0.055100947368792674 1107 24-25 0.035706792326941184 1107 26-27 0.15273145194650084 1107 28-29 0.16239445309924605 1107 30-31 0.09323476761777982 1107 32-33 0.17012355068576568 1107 34-35 0.1282220130436471 1107 36-37 0.16102276511875147 1107 38-39 0.20628081688061428 1107 40-41 0.22521824005875857 1107 42-43 0.26936902643632266 1107 44-45 0.20436375187100708 1107 46-47 0.14147548431024148 1107 48-49 0.17591004352411943 1107 50-51 0.1954977093446999 1107 52-53 0.3232376435855997 1107 54-55 0.46314207503596094 1107 56-57 0.37698825038078354 1107 58-59 0.35234225486993154 1107 60-61 0.40940293219722435 1107 62-63 0.48369856459142824 1107 64-65 0.34257933638196647 1107 66-67 0.20860447885361566 1107 68-69 0.25808496770240197 1107 70-71 0.2973414152434728 1107 72-73 0.32002079437067366 1107 74-75 0.4534232903482156 1107 76-77 0.42799852133006766 1107 78-79 0.5497365556841096 1107 80-81 0.6677230344595166 1107 82-83 0.5210926705920222 1107 84-85 0.595435453059654 1107 86-87 0.4322240509516817 1107 88-89 0.37971744119131046 1107 90-91 0.570070429005181 1107 92-93 0.6859642996847448 1107 94-95 0.7494076300008032 1107 96-97 0.7998969183526263 1107 98-99 0.6139494040927111 1107 100-101 0.5755477073793998 1108 1 -0.004285233569511604 1108 2 -0.016849500070527768 1108 3 -0.058322650707047785 1108 4 0.06818708263310214 1108 5 0.06910595556504262 1108 6 0.022716551992516543 1108 7 0.06583105466364714 1108 8 0.0157151920036398 1108 9 0.012216439673935042 1108 10-11 0.06959966775078641 1108 12-13 0.01293981086448781 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sequence quality scores pass #Quality Count 2 6.0 3 10.0 4 23.0 5 70.0 6 123.0 7 349.0 8 788.0 9 1534.0 10 2648.0 11 4202.0 12 6214.0 13 8776.0 14 11459.0 15 14906.0 16 19771.0 17 26891.0 18 24822.0 19 16927.0 20 15261.0 21 16109.0 22 17353.0 23 19066.0 24 22435.0 25 26806.0 26 33134.0 27 41105.0 28 51013.0 29 63240.0 30 78367.0 31 98286.0 32 123557.0 33 159000.0 34 213407.0 35 298450.0 36 450331.0 37 644589.0 38 418020.0 39 24425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.889689825025336 19.530449872927967 14.890932096390506 22.688928205656193 2 15.414070882967698 21.198017029492302 39.330596777148095 24.057315310391907 3 19.728488402605077 24.8186790769486 29.93355262378257 25.51927989666375 4 13.029234126954567 17.292090039296205 37.27640677089224 32.402269062856995 5 13.775651165814336 37.71645710540002 33.91242578226576 14.595465946519893 6 31.424902140632398 36.7441991174458 17.295841201189244 14.535057540732554 7 28.07054609945647 33.03994991658972 20.799614555474182 18.089889428479626 8 26.201695427721873 34.610473838765415 19.713943550525094 19.47388718298762 9 25.99547244734333 17.145583232209415 20.676884697061627 36.182059623385626 10-11 24.796441348983787 26.868218133130426 27.245443538685837 21.089896979199946 12-13 26.1194701966126 24.256951053894856 27.78584737358357 21.837731375908973 14-15 22.77769256736053 25.46029708075882 26.5863104216629 25.17569993021775 16-17 21.936361023107374 28.950086897696377 26.846343271125217 22.26720880807104 18-19 21.908238534980303 27.575401342560017 29.140953904265572 21.375406218194108 20-21 22.783331270771285 26.334165863508957 29.286614807480007 21.595888058239755 22-23 22.40779098754407 26.338599840015576 29.29729592462956 21.956313247810797 24-25 22.603480527009438 26.50910089546093 29.167936511806513 21.71948206572312 26-27 22.303120371705976 26.625654944871872 29.33250646225818 21.73871822116397 28-29 21.84260155884962 27.04885117794045 29.310689834973218 21.79785742823671 30-31 22.24044613215006 26.44084668947665 29.2119550955937 22.10675208277959 32-33 22.029528347901344 26.68127502787953 29.25433531589664 22.03486130832248 34-35 22.605031432486435 26.503661960004376 29.050680334643314 21.840626272865876 36-37 22.48295581141309 26.316501456383335 29.14557941437204 22.054963317831536 38-39 22.135081476033303 26.808952153973635 29.16159991764522 21.89436645234784 40-41 22.369581834018554 26.68335910842723 29.00352386587471 21.943535191679512 42-43 22.163822049499014 26.85103266561096 28.565031743984115 22.42011354090591 44-45 22.406113074336552 26.722506012413184 28.49607902289948 22.375301890350784 46-47 22.738349096754927 27.06188533998406 28.133729162589532 22.066036400671482 48-49 22.78762432809921 27.158149574639186 27.78871629914928 22.26550979811233 50-51 22.251110966639175 27.35006182947525 28.08720732581086 22.311619878074712 52-53 22.865995389157103 27.50118250615462 27.38677482407999 22.246047280608288 54-55 22.557096426096056 27.16798137456698 27.602094006834875 22.672828192502084 56-57 22.734094666351773 27.388367027863907 27.48214117927894 22.395397126505376 58-59 23.198468646927264 27.13758561338007 27.35416162668512 22.309784113007545 60-61 22.831220837818954 27.120572313067044 28.043909117519238 22.00429773159476 62-63 22.48277106724405 27.290653187585352 27.98460172070929 22.241974024461307 64-65 22.425305272117914 27.55105305739365 27.655861923841147 22.367779746647294 66-67 22.60385070074072 27.44366216616349 27.308123946874304 22.64436318622149 68-69 22.366694712820365 27.343493151264703 27.719712270164028 22.570099865750905 70-71 22.696627425433586 27.189691617337203 27.635866096529437 22.477814860699773 72-73 22.690033055998818 26.954876513176185 27.789385580975345 22.565704849849652 74-75 22.43071503120479 27.767128354843322 27.29134942004708 22.510807193904803 76-77 22.61656023264814 27.358519952611722 27.321004796725752 22.703915018014385 78-79 22.580574712702056 27.365902655003595 27.418705783305374 22.63481684898898 80-81 22.717550552461777 27.590279327301225 27.278504864026992 22.41366525621001 82-83 22.638195119448742 27.340954408152186 27.541992580516432 22.47885789188264 84-85 22.51926012409371 27.486479311619867 27.273510692619524 22.720749871666897 86-87 22.474006750947986 27.41654175257156 27.38571249114992 22.723739005330536 88-89 22.565024383191425 27.344979750930175 27.031213125988458 23.058782739889942 90-91 22.62331965724859 27.213947067034262 27.254103460272344 22.908629815444804 92-93 22.779615635103053 27.2040222524729 27.24250241961192 22.77385969281213 94-95 22.579976183970533 27.11323245548546 27.14698932409404 23.15980203644997 96-97 22.204184688916317 27.175015858789646 27.555965402020956 23.064834050273085 98-99 22.248353899049526 27.373059388637266 26.976485046057647 23.40210166625556 100-101 22.049056591635626 27.42929367913941 27.047623529841967 23.474026199382998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 590.0 1 452.0 2 340.5 3 381.0 4 344.0 5 333.0 6 588.5 7 1231.0 8 2067.0 9 2570.5 10 2513.0 11 2430.5 12 2629.0 13 2923.5 14 3213.5 15 3463.0 16 3713.0 17 3974.5 18 4236.0 19 4544.5 20 5001.0 21 5680.0 22 6676.5 23 8059.5 24 9766.5 25 12207.0 26 15665.5 27 19613.0 28 23196.0 29 27527.0 30 33594.5 31 39797.5 32 45493.5 33 51300.5 34 57854.5 35 62880.5 36 68823.5 37 76844.5 38 81991.0 39 83989.5 40 85928.5 41 89968.0 42 92936.0 43 96445.0 44 102042.5 45 105403.5 46 106464.5 47 115351.0 48 134488.0 49 133843.5 50 116585.0 51 107647.0 52 102677.0 53 99349.5 54 95635.0 55 88817.0 56 80648.5 57 72677.5 58 66641.0 59 61463.5 60 53522.5 61 43020.5 62 33201.5 63 25166.0 64 18913.0 65 14434.0 66 10283.5 67 7691.0 68 5397.5 69 3128.5 70 1996.0 71 1241.0 72 765.5 73 523.5 74 381.5 75 249.0 76 167.0 77 79.0 78 38.5 79 18.0 80 8.0 81 7.5 82 7.0 83 4.0 84 0.5 85 1.5 86 2.5 87 1.5 88 1.5 89 2.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027662348699310946 2 0.00426616393649104 3 2.708675515232406E-4 4 2.3700910758283554E-4 5 3.3858443940405074E-5 6 0.0 7 0.0 8 0.0 9 3.3858443940405074E-5 10-11 0.011224074166244283 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.724428833444558E-4 20-21 8.972487644207346E-4 22-23 0.0020484358583945073 24-25 0.012087464486724611 26-27 0.005789793913809268 28-29 4.4015977122526597E-4 30-31 0.0029795430667556464 32-33 0.0047909698175673185 34-35 0.0 36-37 0.009040204532088154 38-39 0.013407943800400411 40-41 0.0013712669795864056 42-43 0.0 44-45 0.0 46-47 3.724428833444558E-4 48-49 0.003318127506159697 50-51 0.0063992459047365596 52-53 0.0 54-55 0.003114976842517267 56-57 0.00340277361601071 58-59 2.2007988561263298E-4 60-61 0.0018452851947520766 62-63 0.005281917254703191 64-65 5.0787665910607614E-5 66-67 0.002116152746275317 68-69 2.708675515232406E-4 70-71 0.0010834702060929624 72-73 0.0 74-75 1.6929221970202537E-5 76-77 0.0 78-79 0.0016759929750500514 80-81 0.0018960728606626843 82-83 0.010242179291972535 84-85 0.007482716110829522 86-87 0.0037244288334445583 88-89 1.354337757616203E-4 90-91 6.940981007783041E-4 92-93 1.1850455379141777E-4 94-95 0.0 96-97 0.0021500111902157224 98-99 0.0018452851947520766 100-101 2.2007988561263298E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2953473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.891492939105326 #Duplication Level Percentage of deduplicated Percentage of total 1 72.07625508730095 34.51839461590628 2 14.86855758404318 14.241548411017696 3 5.31688459326426 7.639006228690596 4 2.4936933740512828 4.77706794462683 5 1.3508977798525275 3.2348255742630188 6 0.8461788550588378 2.4314861197362334 7 0.5557196598750067 1.8629970916918124 8 0.3915755053670131 1.5002508440328726 9 0.3068005184858747 1.3223821378302107 >10 1.5862093515201297 13.779216272969103 >50 0.1272762941339617 4.223768402578635 >100 0.07393499860133308 6.403311841067397 >500 0.004176088332977097 1.338773669368584 >1k 0.0015571854799928072 1.5026712408586773 >5k 1.415623163629825E-4 0.4878961214933552 >10k+ 1.415623163629825E-4 0.7364034838686617 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11196 0.3790791383567752 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10528 0.35646169780458464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8666 0.2934172751875504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5727 0.19390730844669987 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4957 0.16783630661258794 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4111 0.13919206303900528 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2959 0.10018713561965863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.3858443940405074E-5 0.0 0.0 0.0 1.0157533182121523E-4 14-15 6.771688788081015E-5 0.0 0.0 0.0 1.0157533182121523E-4 16-17 6.771688788081015E-5 0.0 0.0 1.6929221970202537E-5 1.0157533182121523E-4 18-19 6.771688788081015E-5 0.0 0.0 5.078766591060761E-5 1.6929221970202538E-4 20-21 6.771688788081015E-5 0.0 0.0 8.464610985101269E-5 2.3700910758283554E-4 22-23 6.771688788081015E-5 0.0 0.0 1.862214416722279E-4 2.3700910758283554E-4 24-25 1.0157533182121523E-4 0.0 0.0 3.724428833444558E-4 2.3700910758283554E-4 26-27 1.0157533182121523E-4 0.0 0.0 5.586643250166838E-4 3.047259954636457E-4 28-29 1.6929221970202538E-4 0.0 0.0 0.0014051254235268105 3.047259954636457E-4 30-31 2.0315066364243046E-4 0.0 0.0 0.002861038512964229 3.047259954636457E-4 32-33 2.0315066364243046E-4 0.0 0.0 0.004029154828908204 3.047259954636457E-4 34-35 2.20079885612633E-4 0.0 0.0 0.005451209474405217 3.047259954636457E-4 36-37 2.708675515232406E-4 0.0 0.0 0.007652008330531547 3.047259954636457E-4 38-39 3.047259954636457E-4 0.0 0.0 0.011257932610184688 3.047259954636457E-4 40-41 3.047259954636457E-4 0.0 0.0 0.015371733548943906 3.047259954636457E-4 42-43 3.3858443940405075E-4 0.0 0.0 0.019299313046030893 3.047259954636457E-4 44-45 3.724428833444558E-4 0.0 0.0 0.021110739796842564 3.5551366137425333E-4 46-47 4.2323054925506344E-4 0.0 0.0 0.024056424419657805 4.2323054925506344E-4 48-49 4.4015977122526597E-4 0.0 0.0 0.028085579248566007 5.078766591060761E-4 50-51 4.4015977122526597E-4 0.0 0.0 0.033435213391150014 5.417351030464812E-4 52-53 4.4015977122526597E-4 0.0 0.0 0.04508251810664936 5.417351030464812E-4 54-55 4.740182151656711E-4 0.0 0.0 0.05288688943491272 6.094519909272914E-4 56-57 5.078766591060761E-4 0.0 0.0 0.07311730968930476 6.433104348676965E-4 58-59 5.078766591060761E-4 0.0 0.0 0.11191908644500898 6.771688788081015E-4 60-61 5.078766591060761E-4 0.0 0.0 0.14266255354289678 6.771688788081015E-4 62-63 5.078766591060761E-4 0.0 0.0 0.15778034876228764 6.771688788081015E-4 64-65 5.417351030464812E-4 0.0 0.0 0.18987815361779167 6.771688788081015E-4 66-67 5.417351030464812E-4 0.0 0.0 0.2529225762348259 6.771688788081015E-4 68-69 5.417351030464812E-4 0.0 0.0 0.3463041646224631 6.771688788081015E-4 70-71 5.925227689570888E-4 0.0 0.0 0.42573607410665343 6.940981007783041E-4 72-73 6.094519909272914E-4 0.0 0.0 0.5159349687638926 7.110273227485066E-4 74-75 6.094519909272914E-4 0.0 0.0 0.6197618871071446 7.110273227485066E-4 76-77 6.094519909272914E-4 0.0 0.0 0.6921343110297605 7.448857666889116E-4 78-79 6.094519909272914E-4 0.0 0.0 0.7796414593937375 7.448857666889116E-4 80-81 7.110273227485066E-4 0.0 0.0 0.9037834441012327 7.448857666889116E-4 82-83 7.448857666889116E-4 0.0 0.0 1.0152623707750164 7.787442106293168E-4 84-85 7.448857666889116E-4 0.0 0.0 1.1692167153720383 7.956734325995194E-4 86-87 7.787442106293168E-4 0.0 0.0 1.393122605149937 8.464610985101269E-4 88-89 8.126026545697218E-4 0.0 0.0 1.6718622448893217 8.972487644207345E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12450 0.0 22.550901 1 TACATGG 12675 0.0 21.43448 2 GAGTACT 11210 0.0 21.205149 12-13 ACATGGG 12560 0.0 20.608309 3 AGTACTT 11655 0.0 20.558514 12-13 AGAGTAC 17840 0.0 20.53112 10-11 GTATCAA 25060 0.0 20.037323 1 GTACTTT 12090 0.0 19.387348 14-15 CATGGGG 5980 0.0 17.71084 4 CAACGCA 28650 0.0 17.427345 5 TCAACGC 28655 0.0 17.403011 4 ATCAACG 28705 0.0 17.356152 3 AACGCAG 29040 0.0 17.226019 6 TATCAAC 29020 0.0 17.201653 2 TACTTTT 13875 0.0 17.09858 14-15 GGTATCA 20005 0.0 16.717829 1 ACTTTTT 14240 0.0 16.576923 16-17 AGTACAT 9760 0.0 15.962059 2 GAGTACA 10515 0.0 15.677109 1 ACGCAGA 31880 0.0 15.631847 7 >>END_MODULE