FastQCFastQC Report
Thu 26 May 2016
SRR937104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993064
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99430.33220138293066903No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88670.29625160036671455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72080.2408234504841861No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55920.18683195548107223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45830.1531206816827171No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45130.1507819411813446No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34350.114765337460208No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30290.10120064255224746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG132000.024.5252021
TACATGG133100.023.466122
ACATGGG132050.022.6806813
GTATCAA227100.022.0838471
GAGTACT95650.020.91818212-13
CATGGGG64850.020.6735424
AGAGTAC167850.019.98887810-11
GTACTTT100250.019.93447714-15
AGTACTT100600.019.88890812-13
ATCAACG256650.019.319553
TCAACGC258450.019.149174
CAACGCA258650.019.1153435
TATCAAC261050.019.048552
AACGCAG262950.018.8170536
GGTATCA175400.017.779261
ATGGGGA36350.017.2635755
TACTTTT116200.017.23910114-15
ACGCAGA285750.017.1470097
CGCAGAG286100.016.9934278
ACTTTTT121900.016.80324416-17
Produced by FastQC (version 0.11.5)