##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2993064 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43401611191742 31.0 31.0 34.0 30.0 34.0 2 31.505496708389796 33.0 31.0 34.0 28.0 34.0 3 31.528270026969018 33.0 31.0 34.0 28.0 34.0 4 35.13235166371317 37.0 35.0 37.0 32.0 37.0 5 35.13340042177514 37.0 35.0 37.0 32.0 37.0 6 35.191083785712564 37.0 35.0 37.0 32.0 37.0 7 35.12052799405559 37.0 35.0 37.0 32.0 37.0 8 35.07648082366431 37.0 35.0 37.0 32.0 37.0 9 36.645270198031184 39.0 37.0 39.0 32.0 39.0 10-11 36.500365177624 39.0 37.0 39.0 32.0 39.0 12-13 36.39530126986927 39.0 37.0 39.0 32.0 39.0 14-15 37.46444529752789 40.0 37.0 41.0 32.0 41.0 16-17 37.38824228282456 40.0 37.0 41.0 32.0 41.0 18-19 37.33036396816105 40.0 37.0 41.0 31.5 41.0 20-21 37.2504007598902 40.0 37.0 41.0 31.0 41.0 22-23 37.125867672726 39.0 36.0 41.0 31.0 41.0 24-25 37.07762814293313 39.0 36.0 41.0 31.0 41.0 26-27 37.10798649811698 39.0 36.0 41.0 31.0 41.0 28-29 37.030153381284194 39.0 36.0 41.0 30.5 41.0 30-31 36.88444917983712 39.0 36.0 41.0 30.0 41.0 32-33 36.695143003958485 39.0 36.0 41.0 30.0 41.0 34-35 36.51625608406636 39.0 35.0 41.0 30.0 41.0 36-37 36.331644929744236 39.0 35.0 41.0 30.0 41.0 38-39 36.16725218705648 39.0 35.0 40.5 29.5 41.0 40-41 35.98790319886244 39.0 35.0 40.0 29.0 41.0 42-43 35.767310521926696 38.5 35.0 40.0 27.5 41.0 44-45 35.526200241625304 38.0 35.0 40.0 27.0 41.0 46-47 35.1982394963823 38.0 34.0 40.0 26.0 41.0 48-49 34.976516706625716 38.0 34.0 40.0 25.0 41.0 50-51 34.275083325983005 37.5 33.5 39.5 24.0 40.5 52-53 34.47031319744583 38.0 33.5 39.5 24.0 40.5 54-55 34.86120961663366 38.0 34.0 40.0 25.0 41.0 56-57 34.72526915562113 38.0 34.0 40.0 24.5 41.0 58-59 34.49271649386715 38.0 34.0 40.0 24.0 41.0 60-61 34.18195284163653 38.0 34.0 40.0 23.0 41.0 62-63 33.86062242571492 37.0 33.0 40.0 22.5 41.0 64-65 33.52435831642758 37.0 33.0 40.0 22.0 41.0 66-67 33.13838026851414 36.0 32.5 39.0 21.5 41.0 68-69 32.73220084836141 36.0 32.0 39.0 20.0 41.0 70-71 32.297639809907174 35.0 31.5 38.5 19.0 40.0 72-73 31.905026588138444 35.0 31.0 37.5 18.0 40.0 74-75 31.50486658487757 35.0 31.0 37.0 17.0 39.0 76-77 31.043064899380703 35.0 31.0 36.5 12.5 39.0 78-79 30.571984762103316 34.5 30.0 36.0 8.0 39.0 80-81 30.124921652193205 34.0 30.0 36.0 7.0 37.5 82-83 29.71665824720086 34.0 29.5 35.0 4.0 37.0 84-85 29.33767520507413 34.0 29.0 35.0 2.0 36.5 86-87 28.993477586847458 34.0 29.0 35.0 2.0 36.0 88-89 28.615198004452964 34.0 29.0 35.0 2.0 36.0 90-91 28.227499478794975 34.0 28.5 35.0 2.0 35.5 92-93 27.814464207915368 34.0 27.0 35.0 2.0 35.0 94-95 27.41493365995515 34.0 26.5 35.0 2.0 35.0 96-97 26.87684560036137 33.0 25.5 35.0 2.0 35.0 98-99 26.25511582779386 33.0 25.0 35.0 2.0 35.0 100-101 24.000224184982347 31.0 12.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2733146203515382 1101 2 0.2862010077051309 1101 3 0.30309298259507145 1101 4 0.28792015873882093 1101 5 0.32875895947817924 1101 6 0.30289534752883185 1101 7 0.2218882859821818 1101 8 0.28049619710640883 1101 9 0.23722486063144288 1101 10-11 0.26799899844839103 1101 12-13 0.3825723660682385 1101 14-15 0.4679889018586252 1101 16-17 0.5528411188378328 1101 18-19 0.49422740599134585 1101 20-21 0.4855322360086589 1101 22-23 0.5172311714893638 1101 24-25 0.4988878604004796 1101 26-27 0.47478748315671737 1101 28-29 0.5897856185203878 1101 30-31 0.6177223381607035 1101 32-33 0.4387067400139344 1101 34-35 0.6024699604056778 1101 36-37 0.5131318259317865 1101 38-39 0.48951006869603475 1101 40-41 0.5561762288589733 1101 42-43 0.5826098005705447 1101 44-45 0.7357128647185007 1101 46-47 0.5479003278636014 1101 48-49 0.5350643524523875 1101 50-51 0.5262917145118422 1101 52-53 0.551317768567479 1101 54-55 0.6295756146968188 1101 56-57 0.6566950597632157 1101 58-59 0.757113614876026 1101 60-61 0.69955989059644 1101 62-63 0.6785276018920143 1101 64-65 0.3284776911346228 1101 66-67 0.40008931012749116 1101 68-69 0.36262227943592507 1101 70-71 0.42252205712992463 1101 72-73 0.5318420275870572 1101 74-75 0.5496199192040798 1101 76-77 0.4541131736336048 1101 78-79 0.5691801629102713 1101 80-81 0.6302611595039842 1101 82-83 0.49276515692551826 1101 84-85 0.49425210862882096 1101 86-87 0.6127069691282365 1101 88-89 0.7096333891612865 1101 90-91 0.7833178169142307 1101 92-93 0.7670230061541545 1101 94-95 0.8654638877799528 1101 96-97 0.8663320220349675 1101 98-99 0.6342203180639601 1101 100-101 0.9637053874040582 1102 1 0.27574712094977016 1102 2 0.15788761054052713 1102 3 0.1923489313470199 1102 4 0.2895090766353974 1102 5 0.3091000371375827 1102 6 0.24205833798913545 1102 7 0.1267602048798011 1102 8 0.043744469632613914 1102 9 0.1823179629297087 1102 10-11 0.11338514748796769 1102 12-13 0.14934139856178774 1102 14-15 0.06417361470807492 1102 16-17 0.33344488072140877 1102 18-19 0.13299379952994173 1102 20-21 -0.0016359666837502118 1102 22-23 -0.025884814133661393 1102 24-25 -0.010351039044401489 1102 26-27 0.06315438618266 1102 28-29 -0.06073279185110181 1102 30-31 -0.004411156244707115 1102 32-33 -0.03596384147941478 1102 34-35 -0.13508981916459817 1102 36-37 0.08927552784939508 1102 38-39 0.09268502435680404 1102 40-41 -0.022316934325829152 1102 42-43 0.10027335642505619 1102 44-45 0.16486658761261452 1102 46-47 0.1895522372164109 1102 48-49 0.19945881000098353 1102 50-51 0.02097571688377542 1102 52-53 0.15824537522860993 1102 54-55 -0.10961157425898449 1102 56-57 0.032205509394948706 1102 58-59 -0.10292796669887849 1102 60-61 -0.15429761606488057 1102 62-63 -0.2586055681620323 1102 64-65 -0.031805337052723814 1102 66-67 -0.28707272854325794 1102 68-69 -0.14645350223066345 1102 70-71 -0.16823629787842265 1102 72-73 -0.16254133741427168 1102 74-75 -0.00810695111216475 1102 76-77 -0.1653403172079777 1102 78-79 -0.367315744206266 1102 80-81 -0.25501213141334134 1102 82-83 -0.09165690682661776 1102 84-85 -0.19093784686909743 1102 86-87 -0.15981075377987963 1102 88-89 -0.14262685529465813 1102 90-91 -0.1633677025068785 1102 92-93 0.04158723808610176 1102 94-95 0.004661493663174809 1102 96-97 -0.16994472584873677 1102 98-99 -0.10100628509982101 1102 100-101 -0.0015950736593772774 1103 1 0.23282519019034353 1103 2 0.3673340234115763 1103 3 0.2453743592360489 1103 4 0.28227784317378735 1103 5 0.1879862666015839 1103 6 0.17776582272993835 1103 7 0.28446992285923756 1103 8 0.30481091314498343 1103 9 0.2649600860155772 1103 10-11 0.29084313012908325 1103 12-13 0.13831289636627275 1103 14-15 0.5321543227142556 1103 16-17 0.48924718907960596 1103 18-19 0.4855924193986496 1103 20-21 0.42059471991419173 1103 22-23 0.44509431771813723 1103 24-25 0.4331734591763805 1103 26-27 0.4645835656412771 1103 28-29 0.5645360827882442 1103 30-31 0.42133145494878477 1103 32-33 0.36580469020434236 1103 34-35 0.4605728736260417 1103 36-37 0.37667451704184174 1103 38-39 0.48144116809461934 1103 40-41 0.38836890902697974 1103 42-43 0.580144237248625 1103 44-45 0.5254017679038228 1103 46-47 0.43569498304859167 1103 48-49 0.47667184858752165 1103 50-51 0.4959490307843737 1103 52-53 0.4007314683582237 1103 54-55 0.4845696690800878 1103 56-57 0.5306726378010822 1103 58-59 0.5424852884287148 1103 60-61 0.4984360240208261 1103 62-63 0.43571951304086554 1103 64-65 0.500214039726167 1103 66-67 0.37891188466303305 1103 68-69 0.4463889001372081 1103 70-71 0.44168118081721275 1103 72-73 0.4183683724257996 1103 74-75 0.37148278243380517 1103 76-77 0.48460601384548596 1103 78-79 0.595995553488514 1103 80-81 0.4905580184781755 1103 82-83 0.7055687986487165 1103 84-85 0.613480048545135 1103 86-87 0.6763874245282011 1103 88-89 0.6904474596632042 1103 90-91 0.6643198356993913 1103 92-93 0.6537931080953356 1103 94-95 0.7537692156325946 1103 96-97 0.7296672976909875 1103 98-99 0.7316093002605086 1103 100-101 0.8804435069368814 1104 1 0.20686791827073137 1104 2 0.17439696213813605 1104 3 0.08415842271045548 1104 4 0.22788071304124458 1104 5 0.21004267134280497 1104 6 0.18310293617682305 1104 7 0.14023053535576224 1104 8 0.11212093741876572 1104 9 0.2958141964378811 1104 10-11 0.4243501698522678 1104 12-13 0.16390839960619275 1104 14-15 -0.006237660623064301 1104 16-17 0.23129422557065737 1104 18-19 0.2706152780931106 1104 20-21 0.23441489873128063 1104 22-23 0.07168607940035798 1104 24-25 0.05732484091124235 1104 26-27 0.12019746083991834 1104 28-29 0.21749881241085944 1104 30-31 0.34967311880579643 1104 32-33 0.08421190720238059 1104 34-35 0.1354736333402684 1104 36-37 0.08497477027992062 1104 38-39 0.2805572548239468 1104 40-41 0.1624439173739063 1104 42-43 0.2675727756293256 1104 44-45 0.2051556011342015 1104 46-47 0.1634926813466393 1104 48-49 0.27934692699124497 1104 50-51 0.04968775832661976 1104 52-53 0.230394490266967 1104 54-55 0.3279972919645502 1104 56-57 0.31003324242237085 1104 58-59 0.15750541540050023 1104 60-61 0.25517894922822393 1104 62-63 0.06726500360657894 1104 64-65 0.1386189023566189 1104 66-67 -0.08282254595326322 1104 68-69 -0.07561561827976959 1104 70-71 -0.06708822323246721 1104 72-73 0.2182545916641807 1104 74-75 0.2664170775304058 1104 76-77 0.27552828608093094 1104 78-79 0.2833086294164566 1104 80-81 0.12047304795698821 1104 82-83 0.12996878355451358 1104 84-85 0.16878291291633474 1104 86-87 0.23741915434852245 1104 88-89 0.0725358903664528 1104 90-91 0.028840459385314432 1104 92-93 -0.05335830399200603 1104 94-95 0.11667615412617494 1104 96-97 -0.016145660518581906 1104 98-99 -0.1347218074981562 1104 100-101 0.04927762738370234 1105 1 0.03283424741553986 1105 2 0.03648593777963072 1105 3 0.06455344527968876 1105 4 0.07449768673080825 1105 5 0.10463426989512215 1105 6 -0.0162177095878846 1105 7 0.033052055424079185 1105 8 -0.0035917216841596655 1105 9 0.0651774594202692 1105 10-11 0.11275214618506624 1105 12-13 0.12035283049554835 1105 14-15 0.19134946474800785 1105 16-17 0.20870457895198058 1105 18-19 0.22021407440070817 1105 20-21 0.2087309899841756 1105 22-23 0.13242325966955804 1105 24-25 -0.021256347328353797 1105 26-27 0.19423239231891642 1105 28-29 0.186637359033611 1105 30-31 0.23629110912745688 1105 32-33 0.033186328738715076 1105 34-35 -0.11360127211295179 1105 36-37 -0.017954960109243245 1105 38-39 0.2837025925491332 1105 40-41 0.20535738164945627 1105 42-43 0.3271681153877779 1105 44-45 0.2568253990277398 1105 46-47 0.30376208885345335 1105 48-49 0.3052992672783361 1105 50-51 0.1737398912207695 1105 52-53 0.3730184177200968 1105 54-55 0.27288420180961026 1105 56-57 0.3059571170106281 1105 58-59 0.3129834358144663 1105 60-61 0.43156982393848153 1105 62-63 0.35042103630146215 1105 64-65 0.40269901296618826 1105 66-67 0.3437245647783058 1105 68-69 0.24500314468229334 1105 70-71 0.24169641342322024 1105 72-73 0.3455046424957864 1105 74-75 0.5195301270200581 1105 76-77 0.3983787598314912 1105 78-79 0.40043359383433597 1105 80-81 0.23575233712782406 1105 82-83 0.17017686781298025 1105 84-85 0.1689609048646048 1105 86-87 0.3464281820415813 1105 88-89 0.4678381554227755 1105 90-91 0.379640955501781 1105 92-93 0.3133167803884618 1105 94-95 0.4360932172793639 1105 96-97 0.11460266203845393 1105 98-99 -0.0530494070264389 1105 100-101 0.1119745896869837 1106 1 0.40825250643889177 1106 2 0.3530737448769905 1106 3 0.39706845892845877 1106 4 0.34165388933924135 1106 5 0.29121783070797846 1106 6 0.329823540032983 1106 7 0.44528802751992913 1106 8 0.4215917784674943 1106 9 0.5122493429507529 1106 10-11 0.5823818095153683 1106 12-13 0.5775942621000354 1106 14-15 0.4192975994158914 1106 16-17 0.6384995440717844 1106 18-19 0.6883887786773215 1106 20-21 0.5750734224985834 1106 22-23 0.3784056679194876 1106 24-25 0.36883464448238357 1106 26-27 0.23083202835167071 1106 28-29 0.3240465188758961 1106 30-31 0.3094664200161432 1106 32-33 0.5071293072039893 1106 34-35 0.5813538385937562 1106 36-37 0.46997800925684174 1106 38-39 0.3647917645236518 1106 40-41 0.47072134889241113 1106 42-43 0.47278443325250663 1106 44-45 0.3523959176810578 1106 46-47 0.5063841695328577 1106 48-49 0.48916399278637357 1106 50-51 0.5437362515483386 1106 52-53 0.5747912414992911 1106 54-55 0.6594058881911735 1106 56-57 0.6976805019059924 1106 58-59 0.8061226532848949 1106 60-61 0.8559932391644409 1106 62-63 0.765789553134951 1106 64-65 0.6306644360781064 1106 66-67 0.6786532878598948 1106 68-69 0.9081174910577872 1106 70-71 1.0546351456039886 1106 72-73 0.845200729847928 1106 74-75 1.0297209065165625 1106 76-77 1.0807348411175006 1106 78-79 1.123139083673582 1106 80-81 1.0336258585108986 1106 82-83 1.0444204225597211 1106 84-85 1.1476812566996095 1106 86-87 1.238254994295147 1106 88-89 1.3160559291564766 1106 90-91 1.4095636124626516 1106 92-93 1.3918217849380525 1106 94-95 1.5278144790324717 1106 96-97 1.5136187372583514 1106 98-99 1.4970258128704366 1106 100-101 1.794404980951768 1107 1 0.26928687992907996 1107 2 0.27775469388368634 1107 3 0.2586543065067133 1107 4 0.20220842099343628 1107 5 0.27386218430513765 1107 6 0.33236987808857776 1107 7 0.33981029035260946 1107 8 0.3366435619880406 1107 9 0.2961624545370256 1107 10-11 0.3654158237132563 1107 12-13 0.396897711965849 1107 14-15 0.3766735347116068 1107 16-17 0.5867154108795276 1107 18-19 0.6189744402002262 1107 20-21 0.6379948628422127 1107 22-23 0.5630959046131849 1107 24-25 0.534614202258787 1107 26-27 0.6629752672190392 1107 28-29 0.55074567830912 1107 30-31 0.6237820973188875 1107 32-33 0.4955063145329106 1107 34-35 0.44780714482747896 1107 36-37 0.3577207035682832 1107 38-39 0.48943188314665775 1107 40-41 0.5555966607047722 1107 42-43 0.5034500118629595 1107 44-45 0.5176781025703079 1107 46-47 0.6861695291446708 1107 48-49 0.7007097263034652 1107 50-51 0.7279716234413129 1107 52-53 0.7234672324231113 1107 54-55 0.8229950071012695 1107 56-57 0.9128778308524161 1107 58-59 0.7798235317036628 1107 60-61 0.8552310154505207 1107 62-63 0.9030828493175278 1107 64-65 0.9268584731455363 1107 66-67 0.7854399159280874 1107 68-69 0.8213550935413849 1107 70-71 0.7531330644043095 1107 72-73 0.7189879062798603 1107 74-75 0.7675698144923082 1107 76-77 0.7928972717732456 1107 78-79 0.9078729971904593 1107 80-81 0.9499386995534671 1107 82-83 0.8470612680615837 1107 84-85 0.8723560038613236 1107 86-87 0.9357105224482254 1107 88-89 0.7934348880872548 1107 90-91 0.8805283744842889 1107 92-93 0.9361125719182475 1107 94-95 0.8853958009604455 1107 96-97 0.9335517874934851 1107 98-99 1.0893364581959695 1107 100-101 1.135131563850173 1108 1 0.005234677185363523 1108 2 0.15409121072479337 1108 3 0.050835275107367295 1108 4 0.05313887025078401 1108 5 0.10114329919385057 1108 6 0.06388336896907987 1108 7 0.12703585640759485 1108 8 0.04201388185317256 1108 9 -0.06325214510884081 1108 10-11 0.01131502068401602 1108 12-13 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-4.72608486283654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7101.0 3 2788.0 4 2931.0 5 3314.0 6 3736.0 7 4527.0 8 5258.0 9 6697.0 10 8090.0 11 10142.0 12 12069.0 13 14527.0 14 15737.0 15 18582.0 16 22090.0 17 26994.0 18 24935.0 19 18802.0 20 18959.0 21 20808.0 22 22806.0 23 25508.0 24 29086.0 25 33331.0 26 39206.0 27 45782.0 28 53986.0 29 64667.0 30 78230.0 31 95002.0 32 118765.0 33 150645.0 34 199272.0 35 275051.0 36 398982.0 37 562003.0 38 475637.0 39 76998.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.133859754607826 18.708053102461978 14.319556608279271 22.838530534650925 2 15.031489523822255 20.400833482328604 40.2303271936142 24.33734980023494 3 19.511967715373427 24.70645478655146 29.720684243733427 26.06089325434169 4 12.67984478270883 16.654505148784445 37.78710067965225 32.87854938885448 5 13.799549608298337 37.43618503380613 34.03915210785858 14.725113250036953 6 31.402245272845736 36.248138639109385 17.777685610961193 14.571930477083686 7 28.1346745474305 32.826260714396746 21.021595436814437 18.017469301358318 8 26.066474482290936 34.222985817568855 20.119570738440764 19.590968961699446 9 26.14808276747475 17.097120389965998 20.75244982497668 36.00234701758257 10-11 24.67693146114092 26.63278688250541 27.53955032945025 21.15073132690342 12-13 26.02839682130524 24.132851494079482 27.825514812745674 22.01323687186961 14-15 22.85302939051133 25.251448363636182 26.587742361353833 25.307779884498654 16-17 22.04415034208008 28.790199465093526 26.81030676060679 22.355343432219605 18-19 21.908394534969805 27.535295585642704 28.96149415959457 21.594815719792923 20-21 22.881707504194516 26.31295239999104 28.96255842150533 21.842781674309116 22-23 22.45719023853552 26.34847782501647 28.968882733488115 22.225449202959897 24-25 22.624581725936324 26.502532992048096 28.84734034581945 22.025544936196134 26-27 22.30294272421253 26.576048914541552 29.044412332211262 22.076596029034658 28-29 21.903965726531965 26.975537519350162 29.010675041239693 22.109821712878176 30-31 22.214314675476786 26.512623201109687 28.893808273955734 22.379253849457793 32-33 21.987758980623397 26.678880065007444 28.97250080858355 22.36086014578561 34-35 22.61005801777287 26.54814299022078 28.724444313763755 22.117354678242602 36-37 22.450712601970327 26.353626326261608 28.823901861235157 22.37175921053291 38-39 22.14205490754772 26.775987048344685 28.88614394399689 22.19581410011071 40-41 22.4064330504264 26.614819886148922 28.808108773656745 22.17063828976794 42-43 22.134442157596492 26.76654549342457 28.361454880786912 22.737557468192023 44-45 22.36724572306429 26.63465086803331 28.343888233935065 22.654215174967337 46-47 22.72345398812669 26.973164689591485 28.017395833733573 22.285985488548256 48-49 22.643045066214196 27.029337784531943 27.777336941913628 22.550280207340233 50-51 22.153695751826593 27.190090624670066 28.08763369511033 22.568579928393014 52-53 22.721277476908263 27.352672550481564 27.420666814906415 22.505383157703758 54-55 22.365634908409497 27.04154377134135 27.678856345876312 22.913964974372835 56-57 22.584474196222516 27.22993266923658 27.493862846523214 22.691730288017688 58-59 23.00085417839916 26.98191357010987 27.41149196549407 22.6057402859969 60-61 22.690190834836002 26.93563696977655 28.07753206056651 22.296640134820933 62-63 22.419652244197234 27.106588655476433 28.01277458021594 22.46098452011039 64-65 22.2929446840685 27.367738406818447 27.70661261607715 22.632704293035896 66-67 22.4341449444484 27.354656379419755 27.293619293667415 22.917579382464428 68-69 22.223234254111514 27.194069352001364 27.764097706349496 22.818598687537627 70-71 22.534296203870927 27.063760226181532 27.659935431624465 22.74200813832308 72-73 22.49996658826045 26.8539840158238 27.857923259916607 22.788126135999146 74-75 22.20960249703484 27.609981656845168 27.44888802455467 22.73152782156533 76-77 22.405052166128257 27.16139135072527 27.45673374305212 22.97682274009435 78-79 22.400239544731335 27.231604130943545 27.547928161978756 22.82022816234636 80-81 22.48312167689076 27.411951449274756 27.394113964309984 22.710812909524495 82-83 22.441478278160524 27.193497233700892 27.68442968787858 22.680594800260003 84-85 22.404470275046684 27.394669077384272 27.301477088019315 22.899383559549733 86-87 22.363754702903496 27.298321367369976 27.539567888495547 22.79835604123098 88-89 22.405690255509306 27.245830228922514 27.197112287918184 23.151367227649995 90-91 22.43741823609905 27.047164192274604 27.481762096043717 23.033655475582634 92-93 22.656790923987476 27.05132372442734 27.45572335442123 22.836161997163956 94-95 22.44286509310345 26.88105250091096 27.504114568705823 23.171967837279773 96-97 22.052824660575084 27.00779940910884 27.777164227008733 23.162211703307342 98-99 22.23759657432697 27.106085703323135 27.25879312672878 23.397524595621118 100-101 22.12543964660763 26.969789827230734 27.485471329415777 23.419299196745857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 371.0 1 285.0 2 257.5 3 342.0 4 337.5 5 345.0 6 580.0 7 1190.5 8 2035.0 9 2485.5 10 2332.0 11 2229.5 12 2469.5 13 2766.5 14 2959.0 15 3093.5 16 3243.0 17 3469.5 18 3703.5 19 3939.0 20 4427.5 21 5147.5 22 6217.5 23 7617.0 24 9406.0 25 11826.0 26 15224.0 27 19235.0 28 22926.5 29 27321.5 30 33549.0 31 40217.5 32 46026.5 33 51725.0 34 58799.5 35 64316.5 36 70367.5 37 78241.5 38 83157.0 39 85229.0 40 87203.0 41 91330.5 42 94530.5 43 98553.0 44 104273.5 45 107542.5 46 108711.5 47 116636.0 48 134365.5 49 134421.0 50 118534.5 51 109717.5 52 104582.5 53 101045.5 54 96880.0 55 89740.0 56 82138.0 57 74388.5 58 68293.5 59 63318.5 60 55289.5 61 44465.5 62 33872.0 63 25531.0 64 19346.5 65 14887.0 66 10728.5 67 7995.5 68 5708.0 69 3403.5 70 2183.5 71 1426.0 72 938.5 73 595.5 74 416.0 75 296.0 76 182.5 77 102.5 78 57.5 79 36.5 80 28.5 81 27.0 82 22.0 83 17.0 84 16.5 85 13.0 86 12.5 87 12.5 88 8.5 89 4.0 90 2.5 91 1.5 92 1.0 93 0.5 94 1.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034646769998904134 2 0.02669505229423761 3 0.02271919344190435 4 0.046340472505766665 5 0.09281458732589748 6 0.11078947860787473 7 0.0549604017822539 8 0.0846289955710937 9 0.06795711685416683 10-11 0.07004527801610656 12-13 0.06765642164684751 14-15 0.06159240163257451 16-17 0.050550205408237174 18-19 0.059821640967249616 20-21 0.05536132872534633 22-23 0.031105248668254336 24-25 0.008670045144373792 26-27 0.0029735414946021866 28-29 0.006515062825251983 30-31 0.004761007449222603 32-33 0.005880261832022302 34-35 0.006281188775114733 36-37 0.005312281995974694 38-39 0.0029234256267156333 40-41 0.0069159897683444115 42-43 0.003474700173467724 44-45 0.002088161161939738 46-47 0.0016204130616652367 48-49 4.1763223238794756E-4 50-51 0.0020046347154621482 52-53 0.005496040178225391 54-55 0.01139300729954321 56-57 0.007584201340165128 58-59 0.0043266699275391375 60-61 0.0019044029796890412 62-63 0.008202297044099291 64-65 0.0021883928977128454 66-67 0.020747969305033236 68-69 0.04286577233229894 70-71 0.055511676329006 72-73 0.003474700173467724 74-75 0.013180473254163626 76-77 0.020213400047576664 78-79 0.024089027164136818 80-81 0.07255107141043425 82-83 0.02669505229423761 84-85 0.010607858702653869 86-87 0.0299525837068636 88-89 0.045204512833671454 90-91 0.04069408472388161 92-93 0.040811021748950245 94-95 0.011509944324611837 96-97 0.015552624334127168 98-99 0.035682497935226244 100-101 0.07241742909607012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2993064.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.50284787448254 #Duplication Level Percentage of deduplicated Percentage of total 1 74.55099210941817 37.65037413393694 2 13.604135927859442 13.740952144569357 3 4.822769642126524 7.306908047099651 4 2.2335259816244952 4.5119769149474465 5 1.2636631952260402 3.190929505654162 6 0.7844089003079744 2.376893001818626 7 0.5176403986530701 1.8299620014803748 8 0.35202538492464086 1.4222627570244235 9 0.2637361776485046 1.1987485252901935 >10 1.4225890186147907 13.171372883946397 >50 0.11329606684518383 3.9841299991847183 >100 0.06644249369172303 6.096142163899219 >500 0.0031168200528459396 1.055774234403485 >1k 0.001392621725564976 1.4049192156672907 >5k 2.652612810599954E-4 1.0586544710778103 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9943 0.33220138293066903 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8867 0.29625160036671455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7208 0.2408234504841861 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5592 0.18683195548107223 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4583 0.1531206816827171 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4513 0.1507819411813446 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3435 0.114765337460208 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3029 0.10120064255224746 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.341057859103581E-5 6 0.0 0.0 0.0 0.0 3.341057859103581E-5 7 3.341057859103581E-5 0.0 0.0 0.0 3.341057859103581E-5 8 3.341057859103581E-5 0.0 0.0 0.0 3.341057859103581E-5 9 3.341057859103581E-5 0.0 0.0 0.0 3.341057859103581E-5 10-11 3.341057859103581E-5 0.0 0.0 0.0 6.682115718207161E-5 12-13 3.341057859103581E-5 0.0 0.0 1.6705289295517904E-5 1.6705289295517904E-4 14-15 6.682115718207161E-5 0.0 0.0 3.341057859103581E-5 1.8375818225069694E-4 16-17 8.352644647758952E-5 0.0 0.0 5.011586788655371E-5 2.3387405013725065E-4 18-19 1.5034760365966113E-4 0.0 0.0 6.682115718207161E-5 2.6728462872828646E-4 20-21 2.1716876084173275E-4 0.0 0.0 1.0023173577310742E-4 3.174004966148402E-4 22-23 2.3387405013725065E-4 0.0 0.0 1.3364231436414323E-4 3.341057859103581E-4 24-25 2.3387405013725065E-4 0.0 0.0 2.6728462872828646E-4 3.341057859103581E-4 26-27 2.3387405013725065E-4 0.0 0.0 6.180957039341624E-4 4.009269430924297E-4 28-29 2.6728462872828646E-4 0.0 0.0 0.0013364231436414323 4.343375216834655E-4 30-31 2.6728462872828646E-4 0.0 0.0 0.0032074155447394375 4.677481002745013E-4 32-33 2.6728462872828646E-4 0.0 0.0 0.005345692574565729 5.011586788655372E-4 34-35 2.8398991802380434E-4 0.0 0.0 0.007183274397072698 5.345692574565729E-4 36-37 3.0069520731932227E-4 0.0 0.0 0.009271435559012437 5.345692574565729E-4 38-39 3.0069520731932227E-4 0.0 0.0 0.013046830939799484 5.679798360476088E-4 40-41 3.0069520731932227E-4 0.0 0.0 0.016638468138335832 5.679798360476088E-4 42-43 3.174004966148402E-4 0.0 0.0 0.020313631783349773 6.013904146386445E-4 44-45 3.341057859103581E-4 0.0 0.0 0.02223474005233433 6.180957039341624E-4 46-47 3.341057859103581E-4 0.0 0.0 0.02542545030777825 6.515062825251983E-4 48-49 3.675163645013939E-4 0.0 0.0 0.030102931310523263 7.01622150411752E-4 50-51 3.675163645013939E-4 0.0 0.0 0.03468018057749517 7.684433075938236E-4 52-53 3.675163645013939E-4 0.0 0.0 0.04580590324831009 8.352644647758952E-4 54-55 3.675163645013939E-4 0.0 0.0 0.053557157481430404 8.68675043366931E-4 56-57 4.343375216834655E-4 0.0 0.0 0.07296870364282221 8.853803326624489E-4 58-59 4.343375216834655E-4 0.0 0.0 0.11035514108619127 9.020856219579669E-4 60-61 4.343375216834655E-4 0.0 0.0 0.142312359508517 9.020856219579669E-4 62-63 4.343375216834655E-4 0.0 0.0 0.15881718533248873 9.020856219579669E-4 64-65 4.677481002745013E-4 0.0 0.0 0.19067417201904135 9.354962005490026E-4 66-67 5.011586788655372E-4 0.0 0.0 0.25388698671328114 9.354962005490026E-4 68-69 5.011586788655372E-4 0.0 0.0 0.34337722146937055 9.354962005490026E-4 70-71 5.011586788655372E-4 0.0 0.0 0.4244145798419279 9.354962005490026E-4 72-73 5.011586788655372E-4 0.0 0.0 0.5140050463337904 0.0010023173577310743 74-75 5.011586788655372E-4 0.0 0.0 0.6162748274009511 0.0010691385149131458 76-77 5.011586788655372E-4 0.0 0.0 0.6820769619359961 0.0011025490935041816 78-79 5.011586788655372E-4 0.0 0.0 0.7668563051107493 0.0011526649613907355 80-81 5.345692574565729E-4 0.0 0.0 0.8869506298562275 0.0011693702506862533 82-83 6.013904146386445E-4 0.0 0.0 0.9916760884498292 0.001202780829277289 84-85 6.013904146386445E-4 0.0 0.0 1.138883097721933 0.001202780829277289 86-87 6.180957039341624E-4 0.0 0.0 1.3611803823773898 0.001219486118572807 88-89 6.348009932296804E-4 0.0 0.0 1.6382710159221454 0.0012361914078683248 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13200 0.0 24.525202 1 TACATGG 13310 0.0 23.46612 2 ACATGGG 13205 0.0 22.680681 3 GTATCAA 22710 0.0 22.083847 1 GAGTACT 9565 0.0 20.918182 12-13 CATGGGG 6485 0.0 20.673542 4 AGAGTAC 16785 0.0 19.988878 10-11 GTACTTT 10025 0.0 19.934477 14-15 AGTACTT 10060 0.0 19.888908 12-13 ATCAACG 25665 0.0 19.31955 3 TCAACGC 25845 0.0 19.14917 4 CAACGCA 25865 0.0 19.115343 5 TATCAAC 26105 0.0 19.04855 2 AACGCAG 26295 0.0 18.817053 6 GGTATCA 17540 0.0 17.77926 1 ATGGGGA 3635 0.0 17.263575 5 TACTTTT 11620 0.0 17.239101 14-15 ACGCAGA 28575 0.0 17.147009 7 CGCAGAG 28610 0.0 16.993427 8 ACTTTTT 12190 0.0 16.803244 16-17 >>END_MODULE