FastQCFastQC Report
Thu 26 May 2016
SRR937104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993064
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112970.37743930634293155No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104980.3507442540486939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88760.29655229557403384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56870.19000596044722065No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48010.16040418781556293No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41640.1391216492530731No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30150.10073289445197296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126800.023.4927271
TACATGG127200.022.2942312
GAGTACT114300.021.59050212-13
ACATGGG126950.021.401633
GTATCAA254850.020.5997891
GTACTTT119850.020.450614-15
AGTACTT121900.020.38080812-13
AGAGTAC183800.020.3803810-11
TCAACGC287250.018.2220754
CAACGCA288150.018.1651615
ATCAACG290000.018.082343
CATGGGG61850.017.970184
AACGCAG293100.017.8607676
TATCAAC293300.017.8473952
TACTTTT136850.017.80602514-15
ACTTTTT141800.017.33475316-17
GGTATCA202300.017.2849061
GTGTAGC14150.017.1237491
GTATAGG9500.017.0035821
AGTACAT98200.016.3980982
Produced by FastQC (version 0.11.5)