FastQCFastQC Report
Thu 26 May 2016
SRR937103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450272
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.5785121687517928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60640.41812846141965093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37880.26119238322190597No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16100.11101365812757882No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC16000.11032413230069944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60950.035.8698771
GTATCAA97900.035.196631
CATTCCG3750.032.9513249
ATCAACG116950.028.9757753
TATCAAC118250.028.7386022
TCAACGC117950.028.7301144
CAACGCA118450.028.5637845
AACGCAG120850.028.1144876
TACATGG84450.024.9887262
GTACATG86350.024.9884471
ACGCAGA135250.024.839227
CGCAGAG134700.024.6971158
ACATGGG83700.024.6439343
GTATAGG3950.024.0646021
GAGTACT65550.023.16122212-13
GCAGAGT140650.022.8421929
CATGGGG29550.022.1956254
CATGGGA45600.021.5750244
AGTACTT69500.021.46881912-13
GTACTTT71650.020.95760714-15
Produced by FastQC (version 0.11.5)