FastQCFastQC Report
Thu 26 May 2016
SRR937103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450272
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90270.6224349639240088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70950.48921857417091413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.3215948456565389No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19080.1315615277685841No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.10970355905650803No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC15180.10467002052028862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69500.036.026461
GTATCAA110050.034.0198141
TATATCG650.005845730729.2295825
TCAACGC133000.027.8917244
ATCAACG133200.027.8498443
CAACGCA134900.027.5340925
TATCAAC135300.027.4877972
AACGCAG138250.026.8678286
GAGTACT81850.024.57639712-13
TACATGG77550.024.2543322
GTACATG80800.024.1671941
ACGCAGA153350.024.0673457
CGCAGAG152750.024.0374958
ACATGGG77800.023.1385213
GCAGAGT160300.022.609039
GTACTTT88900.022.57477214-15
AGAGTAC125900.022.14640810-11
AGTACTT87900.021.91217212-13
CATGGGG27000.021.4620914
GTGTAGC8000.021.3800281
Produced by FastQC (version 0.11.5)