FastQCFastQC Report
Thu 26 May 2016
SRR937102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081979
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65000.6007510312122509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.42098783802643114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.27726970671334655No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12600.11645327681960556No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC12030.11118515239205197No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.1007413267725159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3600.042.241599
GTATCAA73850.034.2337041
GGTATCA45850.032.441261
TTAGGCA5900.028.1921944
ATCAACG89800.027.8369873
TCAACGC90150.027.6761954
TATCAAC90900.027.6569732
CAACGCA91400.027.2989545
AACGCAG93350.026.77596
CCTTTAG6600.026.6410031
TAGGCAT6150.025.5018565
GGCATTC6200.024.5262377
ACATGGG57300.024.3010963
GTACATG60400.024.2329641
TACATGG59350.024.0222342
ACGCAGA104600.023.8052237
CGCAGAG104900.023.6023358
GCATTCC6800.023.0620448
GAGTACT51750.022.72518212-13
GCAGAGT108150.022.3218369
Produced by FastQC (version 0.11.5)