##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937102_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1081979 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92322032128165 33.0 31.0 34.0 30.0 34.0 2 32.032991398169465 33.0 31.0 34.0 30.0 34.0 3 32.03897118151092 33.0 31.0 34.0 30.0 34.0 4 35.63667224594932 37.0 35.0 37.0 33.0 37.0 5 35.65655248392067 37.0 35.0 37.0 33.0 37.0 6 35.722650809304064 37.0 35.0 37.0 33.0 37.0 7 35.67563418513668 37.0 35.0 37.0 33.0 37.0 8 35.628963223870336 37.0 35.0 37.0 33.0 37.0 9 37.28088900061831 39.0 37.0 39.0 34.0 39.0 10-11 37.137381132166155 39.0 37.0 39.0 33.5 39.0 12-13 37.097914099996395 39.0 37.0 39.0 33.0 39.0 14-15 38.27039803914863 40.0 38.0 41.0 33.0 41.0 16-17 38.21928429294839 40.0 38.0 41.0 33.0 41.0 18-19 38.25039903731958 40.0 38.0 41.0 33.5 41.0 20-21 38.2023472729138 40.0 38.0 41.0 33.5 41.0 22-23 38.09463954476011 40.0 38.0 41.0 33.0 41.0 24-25 37.9545467148623 40.0 38.0 41.0 33.0 41.0 26-27 38.030407244502896 40.0 38.0 41.0 33.0 41.0 28-29 37.97639279505425 40.0 38.0 41.0 33.0 41.0 30-31 37.81407587393101 40.0 37.5 41.0 33.0 41.0 32-33 37.58563752161548 40.0 37.0 41.0 33.0 41.0 34-35 37.42171798158744 40.0 37.0 41.0 32.0 41.0 36-37 37.240152535307985 40.0 36.0 41.0 31.5 41.0 38-39 37.08391521462062 40.0 36.0 41.0 31.0 41.0 40-41 36.92373511870379 39.5 36.0 41.0 31.0 41.0 42-43 36.68586358884969 39.0 36.0 41.0 30.5 41.0 44-45 36.368549666860446 39.0 36.0 41.0 30.0 41.0 46-47 35.96447204613028 39.0 35.0 41.0 29.5 41.0 48-49 35.595191773592646 39.0 35.0 40.5 27.5 41.0 50-51 34.93503385925235 38.5 34.5 40.0 26.5 40.5 52-53 35.169741741752844 38.5 35.0 40.0 27.0 40.5 54-55 35.572865554691916 39.0 35.0 41.0 27.0 41.0 56-57 35.50081008965978 39.0 35.0 41.0 27.0 41.0 58-59 35.29832325766027 39.0 35.0 41.0 26.0 41.0 60-61 35.07964110209163 39.0 35.0 41.0 26.0 41.0 62-63 34.79719476995395 38.5 35.0 40.0 26.0 41.0 64-65 34.5214380316069 38.0 34.0 40.0 25.0 41.0 66-67 34.15791341606445 37.5 34.0 40.0 23.5 41.0 68-69 33.776347322822346 37.0 34.0 40.0 22.5 41.0 70-71 33.37590378371484 36.5 33.5 39.0 22.0 41.0 72-73 32.99837519951866 36.0 33.0 39.0 21.5 40.5 74-75 32.56870743332357 35.5 33.0 38.5 20.0 40.0 76-77 32.139140870571424 35.0 32.5 37.5 18.5 39.5 78-79 31.69005082353724 35.0 32.0 37.0 17.5 39.0 80-81 31.2543593729638 35.0 31.5 36.5 12.5 39.0 82-83 30.837543519791048 35.0 31.0 36.0 8.0 37.5 84-85 30.47877777664816 35.0 31.0 35.5 7.0 37.0 86-87 30.17220112405139 34.5 31.0 35.0 3.5 37.0 88-89 29.813536584351453 34.0 31.0 35.0 2.0 36.0 90-91 29.48017198115675 34.0 30.0 35.0 2.0 36.0 92-93 29.152818123087414 34.0 30.0 35.0 2.0 36.0 94-95 28.773952174672523 34.0 29.0 35.0 2.0 35.0 96-97 28.360811993578434 34.0 29.0 35.0 2.0 35.0 98-99 27.8530184042389 34.0 29.0 35.0 2.0 35.0 100-101 25.815084211431092 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36533690556218446 1101 2 0.3891901687645749 1101 3 0.3412109016038123 1101 4 0.29508221791856926 1101 5 0.2849661435984743 1101 6 0.1581436846757498 1101 7 0.21578762471140323 1101 8 0.18648639953198654 1101 9 0.09787290784460367 1101 10-11 0.35238505318258007 1101 12-13 0.2473893814948056 1101 14-15 0.5006927386446236 1101 16-17 0.5258301915641397 1101 18-19 0.4305379260269291 1101 20-21 0.48034093366356245 1101 22-23 0.5157443936049901 1101 24-25 0.42881002062356544 1101 26-27 0.5946751064309268 1101 28-29 0.6433145265097124 1101 30-31 0.5906976092694407 1101 32-33 0.7965849378202705 1101 34-35 0.47712904689969804 1101 36-37 0.5105899401722738 1101 38-39 0.5986238548525975 1101 40-41 0.5306236441063703 1101 42-43 0.467760101489084 1101 44-45 0.8552504748777494 1101 46-47 0.6837839561913412 1101 48-49 0.6799345432777599 1101 50-51 0.5912764336476499 1101 52-53 0.7485093349006107 1101 54-55 0.9141049650309085 1101 56-57 0.9597207059374071 1101 58-59 0.9236405627522686 1101 60-61 0.9899500733260922 1101 62-63 1.011514046829042 1101 64-65 1.0325598753577907 1101 66-67 1.076757023437679 1101 68-69 0.9354086901819159 1101 70-71 0.9018793686756581 1101 72-73 0.8886532710417114 1101 74-75 0.9412754991171255 1101 76-77 0.934958499192664 1101 78-79 1.0259040773934949 1101 80-81 0.8038407029038304 1101 82-83 0.8741647554626368 1101 84-85 1.0361460420152753 1101 86-87 0.9002952277698562 1101 88-89 0.7295306894586098 1101 90-91 0.9757080534091251 1101 92-93 1.0465701197439081 1101 94-95 1.0833508049226275 1101 96-97 1.0666409948551845 1101 98-99 1.0491511621324783 1101 100-101 1.2215719275240744 1102 1 0.26504740712740116 1102 2 0.23641145215278314 1102 3 0.23222986773869891 1102 4 0.2074136866642604 1102 5 0.1799712512774576 1102 6 0.14164974237036887 1102 7 0.15518536163618535 1102 8 0.06527858218816363 1102 9 0.1705034701196766 1102 10-11 0.3453022784027411 1102 12-13 0.36996785430512347 1102 14-15 0.44625690615624336 1102 16-17 0.5596991033310417 1102 18-19 0.27952366883029356 1102 20-21 0.3619780998447837 1102 22-23 0.26682738610993084 1102 24-25 0.334122386523255 1102 26-27 0.34987259702254647 1102 28-29 0.29940355075947167 1102 30-31 0.08075301524447553 1102 32-33 0.1845191696493984 1102 34-35 0.19037534082605845 1102 36-37 0.20543390595904754 1102 38-39 0.2930452820406444 1102 40-41 0.24545730592612358 1102 42-43 0.26847998675301454 1102 44-45 0.3105668281848253 1102 46-47 0.2947452758079905 1102 48-49 0.4299330875576004 1102 50-51 0.45371189144632496 1102 52-53 0.5510310219663452 1102 54-55 0.6233454968371248 1102 56-57 0.7059233649685055 1102 58-59 0.6690966272191545 1102 60-61 0.3933663320746561 1102 62-63 0.511471641067935 1102 64-65 0.507941495561667 1102 66-67 0.2776079235157809 1102 68-69 0.30034545496412335 1102 70-71 0.6304576870530596 1102 72-73 0.6748626643608375 1102 74-75 0.5355197081990468 1102 76-77 0.598099664806778 1102 78-79 0.6562007784784463 1102 80-81 0.7614010171874845 1102 82-83 0.8052729888892642 1102 84-85 0.8419261371307663 1102 86-87 0.766169856086524 1102 88-89 0.6840580424403662 1102 90-91 0.8739887846110292 1102 92-93 0.9289712225468669 1102 94-95 0.9165140088740849 1102 96-97 0.9141888658961008 1102 98-99 1.1700175814533011 1102 100-101 1.2799645339846855 1103 1 0.3820343499666343 1103 2 0.4881982387917567 1103 3 0.48267451344959156 1103 4 0.3162353919379868 1103 5 0.23159363385653364 1103 6 0.1629533582752316 1103 7 0.16870029418458188 1103 8 0.24128198840401183 1103 9 0.2923175261745712 1103 10-11 0.4115315186984745 1103 12-13 0.3812074641982477 1103 14-15 0.46073332352597873 1103 16-17 0.4426305327275415 1103 18-19 0.31782267748366166 1103 20-21 0.4828052393125901 1103 22-23 0.5194862362003292 1103 24-25 0.5287496388752899 1103 26-27 0.5496858786391527 1103 28-29 0.5032649952181387 1103 30-31 0.36563227178372415 1103 32-33 0.5120388833697618 1103 34-35 0.48578293819690543 1103 36-37 0.3238946606426012 1103 38-39 0.40643527078503183 1103 40-41 0.5343211458930455 1103 42-43 0.7470565954352608 1103 44-45 0.7855206408166993 1103 46-47 0.7458506552007194 1103 48-49 0.8224522926426445 1103 50-51 0.6256819016518378 1103 52-53 0.6179834268269317 1103 54-55 0.7185726927470526 1103 56-57 0.8638533997197868 1103 58-59 0.793239330599441 1103 60-61 0.823586489128111 1103 62-63 0.9403496571933161 1103 64-65 0.7185300522515377 1103 66-67 0.7439465204042435 1103 68-69 0.7871906690339614 1103 70-71 0.7272099685285554 1103 72-73 0.592350120757672 1103 74-75 0.45098000675156413 1103 76-77 0.5628225241904481 1103 78-79 0.6163368958517914 1103 80-81 0.7983369652344905 1103 82-83 0.8149620814510463 1103 84-85 0.6178977126204508 1103 86-87 0.6958838389635602 1103 88-89 0.7794210111642208 1103 90-91 0.6918817546146734 1103 92-93 0.6240016914429951 1103 94-95 0.5648136082007191 1103 96-97 0.6531006160281798 1103 98-99 0.9076310809627159 1103 100-101 1.078374720473363 1104 1 0.35543609566137135 1104 2 0.2664468591360212 1104 3 0.33407679283035563 1104 4 0.3628787775184108 1104 5 0.28872220964962025 1104 6 0.32428896557512843 1104 7 0.3987634470314845 1104 8 0.3741611085181731 1104 9 0.24562483264571 1104 10-11 0.42771806130247825 1104 12-13 0.35143948226622257 1104 14-15 0.47659308011873946 1104 16-17 0.6171047746420015 1104 18-19 0.5475459922153334 1104 20-21 0.5132118075180543 1104 22-23 0.40926846909628267 1104 24-25 0.5501265681696239 1104 26-27 0.520499440451978 1104 28-29 0.5190324202603946 1104 30-31 0.6274352410890458 1104 32-33 0.6049365084669276 1104 34-35 0.6134794062008808 1104 36-37 0.66416736260215 1104 38-39 0.7785986135158893 1104 40-41 0.6135271138950884 1104 42-43 0.787655406630293 1104 44-45 0.6670578112752494 1104 46-47 0.7676621712170899 1104 48-49 0.7243473614610636 1104 50-51 0.5817170978588067 1104 52-53 0.693764426057335 1104 54-55 0.8500731239498904 1104 56-57 0.7129676936106222 1104 58-59 0.8543845705454558 1104 60-61 0.918337029581906 1104 62-63 0.9719454170964781 1104 64-65 0.8345124986218906 1104 66-67 0.6923341504902112 1104 68-69 0.8212992006625939 1104 70-71 0.8506848060056882 1104 72-73 0.9502131916180261 1104 74-75 0.8190420883591258 1104 76-77 0.7391603165092384 1104 78-79 0.7740179140319192 1104 80-81 0.8892269833720796 1104 82-83 0.9411793110017825 1104 84-85 0.9000600305194304 1104 86-87 0.8694661871047487 1104 88-89 1.0347612553449146 1104 90-91 1.1105980850204666 1104 92-93 1.1636839548905265 1104 94-95 1.1615321158908252 1104 96-97 1.356420491192054 1104 98-99 1.397769974685719 1104 100-101 1.1722749405221684 1105 1 0.027195354920632298 1105 2 0.22815754523195508 1105 3 0.32769397558688595 1105 4 0.27332580241215965 1105 5 0.274556301938631 1105 6 0.18103987632193963 1105 7 0.13158069050446386 1105 8 0.2372303252759167 1105 9 0.34283946531115816 1105 10-11 0.28211051728304426 1105 12-13 0.33450718111260613 1105 14-15 0.35956780689469525 1105 16-17 0.2265101328690804 1105 18-19 0.15662310211210695 1105 20-21 0.2470877941604286 1105 22-23 0.3934556275662189 1105 24-25 0.4645201625837103 1105 26-27 0.39222834148416297 1105 28-29 0.20729896549415372 1105 30-31 -0.0631252476784141 1105 32-33 0.17815486001519076 1105 34-35 0.3108249587206089 1105 36-37 0.2100234646057899 1105 38-39 0.08566828564703144 1105 40-41 0.2189460574287878 1105 42-43 0.313311903290888 1105 44-45 0.4004158106680862 1105 46-47 0.47666718032456146 1105 48-49 0.4315972855654948 1105 50-51 0.6052864664076836 1105 52-53 0.5906546051708759 1105 54-55 0.674502009802957 1105 56-57 0.7738587075754069 1105 58-59 0.6384047587664625 1105 60-61 0.6489514042024211 1105 62-63 0.4933441724091736 1105 64-65 0.5347344931574085 1105 66-67 0.6466203526760026 1105 68-69 0.7388007185739482 1105 70-71 0.6431578630791606 1105 72-73 0.5614760938645347 1105 74-75 0.6910366238782473 1105 76-77 0.7578154470496123 1105 78-79 0.6405650451794607 1105 80-81 0.6483192723824018 1105 82-83 0.7669857014085828 1105 84-85 0.6373532150974519 1105 86-87 0.5788544688290962 1105 88-89 0.4842356785385995 1105 90-91 0.4385731912742621 1105 92-93 0.4723208704946593 1105 94-95 0.5876838311306543 1105 96-97 0.33600268609187367 1105 98-99 0.48547299532931376 1105 100-101 0.6401692179963625 1106 1 0.1977450188815304 1106 2 0.23522200364165613 1106 3 0.11855436661420882 1106 4 0.13505280337226822 1106 5 0.14535744970855546 1106 6 0.2665220051514794 1106 7 0.1648834748284571 1106 8 0.27644434290705533 1106 9 0.23037656053679 1106 10-11 0.33535607676633106 1106 12-13 0.2443450531559037 1106 14-15 0.23394237048027833 1106 16-17 0.4371098208327666 1106 18-19 0.2766676163828592 1106 20-21 0.20466776710273393 1106 22-23 0.277400486604364 1106 24-25 0.3617712972611997 1106 26-27 0.19969430115947517 1106 28-29 0.269546936167103 1106 30-31 0.3791979499300311 1106 32-33 0.28109983906730207 1106 34-35 0.4553111352938899 1106 36-37 0.41751451811020246 1106 38-39 0.4716282882655136 1106 40-41 0.43668412149441593 1106 42-43 0.4050947652966457 1106 44-45 0.4111246022365549 1106 46-47 0.5383614502032401 1106 48-49 0.7232260387295 1106 50-51 0.8377311508800531 1106 52-53 0.749180233607639 1106 54-55 0.8809159379691422 1106 56-57 0.8021023577456106 1106 58-59 0.9361075823677609 1106 60-61 1.0310123167449774 1106 62-63 0.9943430159109568 1106 64-65 0.879197757973678 1106 66-67 1.0151076857474877 1106 68-69 0.9120245118880774 1106 70-71 0.7797514848824179 1106 72-73 1.023748067330537 1106 74-75 0.9798185912344479 1106 76-77 1.0423161889541284 1106 78-79 0.9901725449612577 1106 80-81 1.199905638795947 1106 82-83 1.2271020604030838 1106 84-85 1.235788396645848 1106 86-87 1.0797502094243647 1106 88-89 0.9630638512714285 1106 90-91 0.9899893560643456 1106 92-93 0.9512791492761856 1106 94-95 1.0027769510573386 1106 96-97 1.013599079126699 1106 98-99 1.2785599181712435 1106 100-101 1.184200978055717 1107 1 -0.05075749288515752 1107 2 0.08395819882672484 1107 3 0.10501657129183783 1107 4 0.1426943831777976 1107 5 0.002596684758422896 1107 6 0.10105038052362403 1107 7 0.06832205371641464 1107 8 0.17712294899207137 1107 9 0.22938241582470198 1107 10-11 0.369940232502465 1107 12-13 0.18654966477274115 1107 14-15 0.1926589600226123 1107 16-17 0.11914260252360265 1107 18-19 0.15102086121574843 1107 20-21 0.27300399397368125 1107 22-23 0.21590544082486218 1107 24-25 0.3432258115099458 1107 26-27 0.28421013420125263 1107 28-29 0.3014866405641783 1107 30-31 0.3999495655802292 1107 32-33 0.22102075637373986 1107 34-35 0.44599944518186163 1107 36-37 0.3913726709550005 1107 38-39 0.31737346771985386 1107 40-41 0.3567845261496032 1107 42-43 0.3629792702376662 1107 44-45 0.6677915109481987 1107 46-47 0.4879825398016848 1107 48-49 0.6313726123628882 1107 50-51 0.37963870730403926 1107 52-53 0.6624770841624752 1107 54-55 0.8927238818851251 1107 56-57 0.9607984834857746 1107 58-59 0.8370683908859817 1107 60-61 0.8845518943984985 1107 62-63 0.8899419781981521 1107 64-65 0.9966246658931723 1107 66-67 0.855995352676004 1107 68-69 1.144426302140701 1107 70-71 1.0629028444050235 1107 72-73 1.0203929939579055 1107 74-75 1.0023578190229685 1107 76-77 0.9283803396733283 1107 78-79 0.9820187099787212 1107 80-81 0.8499766019809059 1107 82-83 0.8125476761984984 1107 84-85 0.7739807827911953 1107 86-87 0.8526056359626146 1107 88-89 0.7471354217692259 1107 90-91 0.8752445264750115 1107 92-93 0.8468540170866241 1107 94-95 0.6127801196460574 1107 96-97 0.5538160614420136 1107 98-99 0.4809941204460273 1107 100-101 0.22950453545668026 1108 1 0.056677503273970586 1108 2 0.13949564926396008 1108 3 0.01716381245977061 1108 4 0.131345577727636 1108 5 0.040659333640689965 1108 6 0.052467623321021506 1108 7 0.15631137686950325 1108 8 0.1897310947434434 1108 9 0.1691591021224852 1108 10-11 0.19641780414053756 1108 12-13 0.24038976036776205 1108 14-15 0.3412727545709018 1108 16-17 0.24675963271518953 1108 18-19 0.2490977096836673 1108 20-21 0.31476823429585465 1108 22-23 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-1.6924386397065092 2316 76-77 -2.1692121279706633 2316 78-79 -2.0393188761506273 2316 80-81 -2.0445233877960263 2316 82-83 -1.9622493959150162 2316 84-85 -2.0035049560298823 2316 86-87 -2.3379237144636846 2316 88-89 -2.3107486077549915 2316 90-91 -2.016678228028251 2316 92-93 -1.766466639643216 2316 94-95 -2.340441168869347 2316 96-97 -2.3384726788716286 2316 98-99 -2.421504397209212 2316 100-101 -2.2804537544286134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1775.0 3 538.0 4 546.0 5 512.0 6 617.0 7 771.0 8 1017.0 9 1194.0 10 1652.0 11 2244.0 12 3381.0 13 4505.0 14 5285.0 15 6901.0 16 9322.0 17 13358.0 18 10832.0 19 6043.0 20 5244.0 21 5311.0 22 5641.0 23 6115.0 24 7123.0 25 8278.0 26 9397.0 27 11394.0 28 13277.0 29 16475.0 30 20449.0 31 25726.0 32 33041.0 33 43356.0 34 60396.0 35 86743.0 36 133343.0 37 221382.0 38 246477.0 39 52308.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.354282311956595 20.802006634541776 15.221262338065774 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32.39085198717181 19.362113387167838 20-21 20.86518665371497 27.61544752923031 32.167557673485106 19.35180814356961 22-23 20.332784347478 27.775756766138958 31.921069916884804 19.970388969498234 24-25 19.910899902663758 28.161417319195394 32.26964449625029 19.658038281890562 26-27 19.81294969348744 28.129275092249557 32.270060119351335 19.787715094911672 28-29 19.55432102474274 28.339599706978667 32.32293354100565 19.783145727272938 30-31 20.112944060384724 27.897848433674135 32.12578672160893 19.863420784332213 32-33 19.840855744670428 27.946694346071745 32.168019288084096 20.044430621173728 34-35 20.180314746655377 28.182405539955223 31.791935564375546 19.84534414901385 36-37 20.46637416225399 27.700497540948 31.749037129328293 20.084091167469715 38-39 19.619941363068346 28.57325734440509 31.654838840866912 20.151962451659656 40-41 20.164051628800955 28.198274264487384 31.67881410331953 19.958860003392132 42-43 19.974647486376373 28.23482546231598 31.371309420820303 20.419217630487346 44-45 20.465835897331626 28.253720762794497 31.02491845884679 20.25552488102709 46-47 20.50516701649508 28.52474077113828 30.61363693078943 20.35645528157721 48-49 20.17321150639758 28.53151467875324 30.67297490138377 20.622298913465414 50-51 20.14146049527348 28.81537810990108 30.270189083834175 20.772972310991264 52-53 20.433326077804228 29.20151746849506 29.817128317036122 20.54802813666459 54-55 20.517034292260846 28.723783309402123 29.804361780740823 20.95482061759621 56-57 20.631846634463614 29.223738804925027 29.55601477747586 20.588399783135504 58-59 20.628356647994295 28.890800132914134 29.748918917295153 20.73192430179642 60-61 20.454398393794072 28.845936971946863 30.170298871144862 20.529365763114203 62-63 20.208603508214086 28.730862837277616 30.35551702113303 20.705016633375266 64-65 20.222767152359015 28.78341830792722 30.13299848783428 20.860816051879485 66-67 20.350573612971388 29.032137301065696 29.50065028881697 21.11663879714594 68-69 20.406961507226494 28.642794512772607 29.879264912315573 21.070979067685325 70-71 20.405442005829137 28.613468021560223 30.010482461289307 20.970607511321333 72-73 20.477789198902585 28.460523785930324 30.065833746219322 20.99585326894777 74-75 20.23595869659766 29.23901332917976 29.475517413346928 21.049510560875653 76-77 20.20643554562918 29.087481118418257 29.599941575717686 21.10614176023488 78-79 20.341653237773237 29.01158721661799 29.208189914600556 21.438569631008217 80-81 20.230862781696832 28.777556055086457 29.34519079784576 21.64639036537095 82-83 20.435042066177093 28.97798210852755 29.47932792111592 21.107647904179437 84-85 20.383946089476492 28.760460069745353 29.28251027808013 21.573083562698024 86-87 20.167578992908304 28.779020456044485 29.434157439021742 21.619243112025465 88-89 20.488056490981243 28.475569906110277 29.44367771891503 21.59269588399345 90-91 20.12485654715768 28.357836442267832 29.94578950019944 21.571517510375056 92-93 20.328992466925797 28.50494570334655 29.858698812467267 21.307363017260386 94-95 20.0796825638928 28.52631165403717 29.735000011554895 21.65900577051514 96-97 20.213781476555738 28.565709628672213 29.58238047888419 21.638128415887856 98-99 20.191952920767406 28.626535147136472 29.1923237326049 21.98918819949122 100-101 20.256461593877546 28.605612617407335 29.30031169904881 21.837614089666303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 349.0 1 253.5 2 191.5 3 220.5 4 198.0 5 223.5 6 366.0 7 672.0 8 1065.5 9 1301.5 10 1240.0 11 1149.0 12 1243.0 13 1335.0 14 1466.5 15 1607.0 16 1746.5 17 1868.0 18 2003.5 19 2240.5 20 2545.0 21 2944.0 22 3471.5 23 4286.0 24 5249.0 25 6955.5 26 10270.0 27 13979.0 28 15485.5 29 16954.0 30 20244.5 31 23841.5 32 27116.0 33 30001.0 34 34507.0 35 39048.0 36 43451.5 37 46744.0 38 44888.5 39 42030.5 40 40929.0 41 39978.5 42 39087.0 43 38696.5 44 38609.5 45 38517.5 46 37567.5 47 36276.0 48 36609.5 49 35285.5 50 31370.5 51 28246.0 52 25970.0 53 23747.5 54 21997.5 55 19827.5 56 17270.5 57 15064.0 58 13451.5 59 12045.0 60 10044.0 61 7885.5 62 5875.5 63 4024.5 64 2599.5 65 1830.5 66 1329.5 67 969.5 68 692.5 69 515.0 70 381.5 71 231.0 72 139.0 73 99.5 74 73.5 75 51.5 76 35.5 77 18.0 78 12.0 79 8.5 80 5.5 81 5.5 82 3.5 83 3.0 84 5.0 85 3.5 86 2.0 87 2.0 88 2.5 89 3.0 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0339193274545994 2 0.03225570921431931 3 0.021534613888069917 4 0.03937229835329521 5 0.05850390811651612 6 0.0643265719574964 7 0.061369028419220706 8 0.06284780018835856 9 0.06210841430378963 10-11 0.06746896196691432 12-13 0.06705305740684431 14-15 0.061692509743719606 16-17 0.050232028532901284 18-19 0.04200636056707201 20-21 0.04847598705705009 22-23 0.02657168022669571 24-25 0.014972564162520715 26-27 0.012107443859816132 28-29 0.013540004011168425 30-31 0.0107210953262494 32-33 0.006238568401050298 34-35 0.008595360908113744 36-37 0.0053605476631247 38-39 0.006192356783264739 40-41 0.005868875458765835 42-43 0.0032348132449890434 44-45 9.242323557111553E-4 46-47 0.0018484647114223106 48-49 6.469626489978087E-4 50-51 0.00475979663191245 52-53 0.004205257218485756 54-55 0.005406759280910257 56-57 0.018253589025295315 58-59 0.007162800756761453 60-61 0.016174066224945216 62-63 0.01090594179739163 64-65 0.00804082149468705 66-67 0.01404833180680956 68-69 0.02130355579914213 70-71 0.01589679651823187 72-73 0.01506498739809183 74-75 0.016589970785015236 76-77 0.02190430683035438 78-79 0.03290267186331713 80-81 0.02749591258240687 82-83 0.02684894993340906 84-85 0.017236933434013044 86-87 0.02037932344343097 88-89 0.016451335931658564 90-91 0.016405124313873008 92-93 0.026109564048840135 94-95 0.017236933434013044 96-97 0.03331857642338715 98-99 0.052496397804393614 100-101 0.0598440450322973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1081979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.381132608343044 #Duplication Level Percentage of deduplicated Percentage of total 1 77.64057716256144 43.77463676790667 2 13.372903114476053 15.07958847711596 3 4.178090296524931 7.066963891740103 4 1.7797008417208753 4.013661966409777 5 0.901818403142301 2.5422771488105127 6 0.48123437083565435 1.6279523326666538 7 0.29838840393261895 1.1776433319641773 8 0.20476944802694488 0.9236106722675512 9 0.13893250894251977 0.7049854989268212 >10 0.834077851836518 9.15904819429242 >50 0.09912752151459121 3.880791466614269 >100 0.0654583938570168 6.857612283826736 >500 0.0036092339276132556 1.3711707889511104 >1k 0.0011483926133314902 1.2188287564765774 >5k 1.6405608761878434E-4 0.6012284220306406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6500 0.6007510312122509 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.42098783802643114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3000 0.27726970671334655 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1260 0.11645327681960556 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1203 0.11118515239205197 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1090 0.1007413267725159 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.242323557111552E-5 7 0.0 0.0 0.0 0.0 9.242323557111552E-5 8 0.0 0.0 0.0 0.0 9.242323557111552E-5 9 0.0 0.0 0.0 0.0 9.242323557111552E-5 10-11 0.0 0.0 0.0 0.0 9.242323557111552E-5 12-13 0.0 0.0 0.0 0.0 1.8484647114223104E-4 14-15 0.0 0.0 0.0 0.0 2.310580889277888E-4 16-17 0.0 0.0 0.0 0.0 2.7726970671334655E-4 18-19 4.621161778555776E-5 0.0 0.0 0.0 2.7726970671334655E-4 20-21 9.242323557111552E-5 0.0 0.0 1.8484647114223104E-4 2.7726970671334655E-4 22-23 9.242323557111552E-5 0.0 0.0 3.2348132449890434E-4 2.7726970671334655E-4 24-25 1.8484647114223104E-4 0.0 0.0 8.780207379255974E-4 2.7726970671334655E-4 26-27 1.8484647114223104E-4 0.0 0.0 0.0016174066224945217 2.7726970671334655E-4 28-29 1.8484647114223104E-4 0.0 0.0 0.003881775893986852 2.7726970671334655E-4 30-31 1.8484647114223104E-4 0.0 0.0 0.00785597502354482 3.2348132449890434E-4 32-33 1.8484647114223104E-4 0.0 0.0 0.012015020624245018 3.6969294228446207E-4 34-35 1.8484647114223104E-4 0.0 0.0 0.016266489460516332 4.621161778555776E-4 36-37 1.8484647114223104E-4 0.0 0.0 0.02213536491928217 4.621161778555776E-4 38-39 1.8484647114223104E-4 0.0 0.0 0.031608746565321505 4.621161778555776E-4 40-41 1.8484647114223104E-4 0.0 0.0 0.04348513233620985 4.621161778555776E-4 42-43 1.8484647114223104E-4 0.0 0.0 0.06446520681085308 4.621161778555776E-4 44-45 1.8484647114223104E-4 0.0 0.0 0.07999231038680049 5.083277956411354E-4 46-47 3.2348132449890434E-4 0.0 0.0 0.11150863371655087 5.545394134266931E-4 48-49 3.6969294228446207E-4 0.0 0.0 0.14898625574063823 5.545394134266931E-4 50-51 3.6969294228446207E-4 0.0 0.0 0.16784059579714578 6.469626489978087E-4 52-53 3.6969294228446207E-4 0.0 0.0 0.20037357471817846 7.393858845689241E-4 54-55 3.6969294228446207E-4 0.0 0.0 0.259570657101478 7.393858845689241E-4 56-57 5.545394134266931E-4 0.0 0.0 0.34982194663667227 7.855975023544819E-4 58-59 5.545394134266931E-4 0.0 0.0 0.47815160922716615 8.318091201400397E-4 60-61 5.545394134266931E-4 0.0 0.0 0.6254742467275243 8.780207379255974E-4 62-63 5.545394134266931E-4 0.0 0.0 0.7782036435087927 9.242323557111552E-4 64-65 5.545394134266931E-4 0.0 0.0 0.9401753638471726 9.242323557111552E-4 66-67 5.545394134266931E-4 0.0 0.0 1.1462329675529745 9.242323557111552E-4 68-69 6.469626489978087E-4 0.0 0.0 1.4090846495172271 9.242323557111552E-4 70-71 6.469626489978087E-4 0.0 0.0 1.5584868098179356 9.242323557111552E-4 72-73 6.469626489978087E-4 0.0 0.0 1.7380189449148276 9.242323557111552E-4 74-75 6.469626489978087E-4 0.0 0.0 1.9371448059527958 9.242323557111552E-4 76-77 6.469626489978087E-4 0.0 0.0 2.146021318343517 0.0010166555912822707 78-79 6.469626489978087E-4 0.0 0.0 2.3810073947830777 0.0010166555912822707 80-81 6.469626489978087E-4 0.0 0.0 2.7270399887613346 0.0010628672090678285 82-83 6.469626489978087E-4 0.0 0.0 3.087906512048755 0.0011090788268533862 84-85 6.931742667833664E-4 0.0 0.0 3.610975813763483 0.0012015020624245017 86-87 7.393858845689241E-4 0.0 0.0 4.270785292505677 0.0012015020624245017 88-89 7.393858845689241E-4 0.0 0.0 5.032121695522742 0.0012015020624245017 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 360 0.0 42.24159 9 GTATCAA 7385 0.0 34.233704 1 GGTATCA 4585 0.0 32.44126 1 TTAGGCA 590 0.0 28.192194 4 ATCAACG 8980 0.0 27.836987 3 TCAACGC 9015 0.0 27.676195 4 TATCAAC 9090 0.0 27.656973 2 CAACGCA 9140 0.0 27.298954 5 AACGCAG 9335 0.0 26.7759 6 CCTTTAG 660 0.0 26.641003 1 TAGGCAT 615 0.0 25.501856 5 GGCATTC 620 0.0 24.526237 7 ACATGGG 5730 0.0 24.301096 3 GTACATG 6040 0.0 24.232964 1 TACATGG 5935 0.0 24.022234 2 ACGCAGA 10460 0.0 23.805223 7 CGCAGAG 10490 0.0 23.602335 8 GCATTCC 680 0.0 23.062044 8 GAGTACT 5175 0.0 22.725182 12-13 GCAGAGT 10815 0.0 22.321836 9 >>END_MODULE