FastQCFastQC Report
Thu 26 May 2016
SRR937102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081979
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70700.6534322754877867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56630.5233927830392272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36540.3377145027768561No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15810.14612113543793365No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13040.12051989918473464No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA11260.10406856325307608No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC11010.10175798236379818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.042.9022141
GTATCAA74800.036.206951
ATCAACG95100.028.271853
TCAACGC96000.028.1031684
TATCAAC96200.028.0005442
CAACGCA97950.027.4951955
TAGACGG700.00839281127.1418915
AACGCAG99600.027.0848886
GAGTACT63900.024.30496212-13
ACGCAGA111200.024.0886467
CGCAGAG111450.023.949388
GTACATG62250.023.3561231
TACATGG62250.022.6660372
GCAGAGT118650.022.2959259
ACATGGG60600.022.2619933
GTACTTT70200.022.02277414-15
AGTACTT66850.021.88252412-13
GTATAGA3700.021.8306121
AGAGTAC99350.021.51209310-11
CATGGGA34950.019.977834
Produced by FastQC (version 0.11.5)