##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937102_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1081979 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12273620837373 33.0 31.0 34.0 31.0 34.0 2 32.202002996361294 33.0 31.0 34.0 30.0 34.0 3 32.3232622814306 34.0 31.0 34.0 30.0 34.0 4 36.027671516729995 37.0 35.0 37.0 35.0 37.0 5 35.93626216405309 37.0 35.0 37.0 35.0 37.0 6 35.974699139262405 37.0 35.0 37.0 35.0 37.0 7 35.92914095375234 37.0 35.0 37.0 35.0 37.0 8 35.910654458173404 37.0 35.0 37.0 35.0 37.0 9 37.57544832200995 39.0 37.0 39.0 35.0 39.0 10-11 37.48987318607847 39.0 37.0 39.0 35.0 39.0 12-13 37.42708638522559 39.0 37.0 39.0 34.0 39.0 14-15 38.61327206905125 40.0 38.0 41.0 34.0 41.0 16-17 38.553377191239385 40.0 38.0 41.0 34.0 41.0 18-19 38.63613757753154 40.0 38.0 41.0 34.0 41.0 20-21 38.72037627347666 40.0 38.0 41.0 34.0 41.0 22-23 38.65558758534131 40.0 38.0 41.0 34.0 41.0 24-25 38.56674112898679 40.0 38.0 41.0 34.0 41.0 26-27 38.38346354226838 40.0 38.0 41.0 34.0 41.0 28-29 38.231607545063255 40.0 38.0 41.0 34.0 41.0 30-31 38.12780839554188 40.0 37.5 41.0 34.0 41.0 32-33 37.99486496503167 40.0 37.0 41.0 33.5 41.0 34-35 37.8523340101795 40.0 37.0 41.0 33.0 41.0 36-37 37.65968979065213 40.0 37.0 41.0 33.0 41.0 38-39 37.489638893176306 40.0 37.0 41.0 33.0 41.0 40-41 37.273008995553525 40.0 36.5 41.0 32.5 41.0 42-43 37.00262897893582 40.0 36.0 41.0 31.5 41.0 44-45 36.676559803840924 40.0 36.0 41.0 31.0 41.0 46-47 36.22893928625232 39.5 36.0 41.0 30.0 41.0 48-49 35.77655296452149 39.0 35.5 41.0 28.5 41.0 50-51 35.53660884360972 39.0 35.0 41.0 27.0 41.0 52-53 35.34775490097313 39.0 35.0 40.0 27.0 41.0 54-55 35.09142506462695 39.0 35.0 40.0 26.0 41.0 56-57 34.86424690312843 38.5 35.0 40.0 25.5 41.0 58-59 34.557722469659765 38.0 34.0 40.0 24.5 41.0 60-61 34.26164879355329 38.0 34.0 40.0 23.5 41.0 62-63 33.962579680381964 38.0 34.0 40.0 23.0 41.0 64-65 33.586288643310084 37.0 33.0 40.0 22.0 41.0 66-67 33.167912685920896 36.5 33.0 39.0 21.5 40.5 68-69 32.7259743488552 36.0 32.0 39.0 19.5 40.0 70-71 32.352237427898324 36.0 32.0 39.0 18.0 40.0 72-73 31.987179972993932 35.0 31.5 38.0 17.5 40.0 74-75 31.504191855849328 35.0 31.0 37.0 13.5 39.0 76-77 30.008704420326087 33.5 29.5 36.0 11.0 38.5 78-79 30.6873867237719 34.0 31.0 36.0 7.5 39.0 80-81 30.280500360912733 34.0 30.0 36.0 7.0 38.0 82-83 30.15054358726001 34.0 30.0 35.5 4.5 37.0 84-85 29.828780872826552 34.0 30.0 35.0 2.0 37.0 86-87 29.48994019292426 34.0 30.0 35.0 2.0 36.0 88-89 29.111557155915225 34.0 29.0 35.0 2.0 36.0 90-91 28.681129670723738 34.0 29.0 35.0 2.0 35.5 92-93 28.29571507395245 34.0 29.0 35.0 2.0 35.0 94-95 27.828616821583413 34.0 28.5 35.0 2.0 35.0 96-97 27.24629498354404 33.0 27.0 35.0 2.0 35.0 98-99 26.55389614770712 33.0 25.5 35.0 2.0 35.0 100-101 23.592462977562413 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28623201388012376 1101 2 0.22598161957063923 1101 3 0.2436077063259745 1101 4 0.24612537584734184 1101 5 0.29340619371307497 1101 6 0.26065153928828266 1101 7 0.24400242904325609 1101 8 0.23379439588645567 1101 9 0.3164726041910697 1101 10-11 0.27778321959623753 1101 12-13 0.3020933951294822 1101 14-15 0.36650691893454024 1101 16-17 0.3338211870851211 1101 18-19 0.32022618247309964 1101 20-21 0.2519059396724259 1101 22-23 0.32671998005005776 1101 24-25 0.35209613095456405 1101 26-27 0.2853471354223842 1101 28-29 0.42540354025912563 1101 30-31 0.36558130371160047 1101 32-33 0.593247184022303 1101 34-35 0.6157174741507774 1101 36-37 0.640692621015134 1101 38-39 0.5132784269574628 1101 40-41 0.6239434251941063 1101 42-43 0.7291162091733199 1101 44-45 0.7770342343781707 1101 46-47 1.0038844319052274 1101 48-49 1.1056223924832693 1101 50-51 1.0693837992775386 1101 52-53 1.1872190561915232 1101 54-55 1.22746108938194 1101 56-57 1.3755608941346722 1101 58-59 1.343465988784196 1101 60-61 1.290655396312495 1101 62-63 1.405797433641382 1101 64-65 1.471895302046427 1101 66-67 1.248133863689688 1101 68-69 1.222087684714218 1101 70-71 1.3110463948808295 1101 72-73 1.2534667616684736 1101 74-75 1.2762525346761464 1101 76-77 1.2334936991320937 1101 78-79 1.3610718976453562 1101 80-81 1.5517164844063913 1101 82-83 1.3274065420714898 1101 84-85 1.3329364577660705 1101 86-87 1.2621660851247327 1101 88-89 1.156345963077566 1101 90-91 1.0410485293039926 1101 92-93 1.1149028244005983 1101 94-95 1.1604775696580631 1101 96-97 1.0375351594008677 1101 98-99 1.176054979377735 1101 100-101 1.2450233748174462 1102 1 0.25483428201871305 1102 2 0.2076387860809703 1102 3 0.17294223967905253 1102 4 0.2090245121622445 1102 5 0.22101588855615262 1102 6 0.25609374276757535 1102 7 0.1956894821213453 1102 8 0.23836358499968924 1102 9 0.2834221869057245 1102 10-11 0.312210368397416 1102 12-13 0.30457280301848044 1102 14-15 0.2632688909669909 1102 16-17 0.3879641033609573 1102 18-19 0.31201009790461853 1102 20-21 0.2301650755822635 1102 22-23 0.2652618801066424 1102 24-25 0.24685553838785523 1102 26-27 0.22978153750748476 1102 28-29 0.222554252865784 1102 30-31 0.2541792173481596 1102 32-33 0.32141093861573466 1102 34-35 0.39463864627133916 1102 36-37 0.45553147279370876 1102 38-39 0.46097883215400515 1102 40-41 0.4049048840535576 1102 42-43 0.40143879169683316 1102 44-45 0.5023221188623452 1102 46-47 0.577621847837392 1102 48-49 0.7770020480978559 1102 50-51 0.8050637535046405 1102 52-53 0.8373931729807822 1102 54-55 0.7772192499592663 1102 56-57 0.8414260286242268 1102 58-59 0.8840560238100963 1102 60-61 0.8687059211945538 1102 62-63 0.8611755601168838 1102 64-65 0.9652159516267318 1102 66-67 0.8462643392544749 1102 68-69 0.7378613620025831 1102 70-71 0.8872338511301088 1102 72-73 0.9503237985564219 1102 74-75 1.038862932062429 1102 76-77 0.8747302004922446 1102 78-79 0.816247615726791 1102 80-81 1.074397743718258 1102 82-83 1.1608816498897205 1102 84-85 1.0974890390110765 1102 86-87 1.0015681259178102 1102 88-89 1.0099210219076475 1102 90-91 0.9530217643065981 1102 92-93 0.9345869457848757 1102 94-95 1.2116281582120507 1102 96-97 1.1103830670246637 1102 98-99 1.319929245663129 1102 100-101 1.502450269184063 1103 1 0.16972236584181388 1103 2 0.16581583711186454 1103 3 0.1853488394683822 1103 4 0.08759029183455169 1103 5 0.16999320342110025 1103 6 0.17793454319549085 1103 7 0.2164180119683934 1103 8 0.18721620217450408 1103 9 0.29240780401162425 1103 10-11 0.1805099185012793 1103 12-13 0.19253808127481165 1103 14-15 0.3641825476611231 1103 16-17 0.31923475641588084 1103 18-19 0.37856707982673754 1103 20-21 0.2573885502505817 1103 22-23 0.16196009857042526 1103 24-25 0.23690679570154316 1103 26-27 0.35281180623100283 1103 28-29 0.36552753802643423 1103 30-31 0.44390634270543217 1103 32-33 0.45755661505148026 1103 34-35 0.547515963433348 1103 36-37 0.587317242162051 1103 38-39 0.6090708754569221 1103 40-41 0.6506282455095018 1103 42-43 0.770981626994157 1103 44-45 0.9986730706666833 1103 46-47 0.9639951795955923 1103 48-49 1.0509251358751825 1103 50-51 0.8478100396464612 1103 52-53 1.0028596243798589 1103 54-55 1.0069714625228912 1103 56-57 0.9868218945676475 1103 58-59 1.0227448768210508 1103 60-61 0.9825253786283383 1103 62-63 0.9673133937242184 1103 64-65 0.9181015546221047 1103 66-67 0.9126613471662139 1103 68-69 0.9198568203814403 1103 70-71 0.8975382283471909 1103 72-73 0.882625668693624 1103 74-75 0.7327661994700634 1103 76-77 0.7615809931429567 1103 78-79 0.7766679946108788 1103 80-81 1.1817316906954858 1103 82-83 1.161369775716249 1103 84-85 1.0330679152223716 1103 86-87 0.9631140655313111 1103 88-89 0.8528843804758566 1103 90-91 0.9091039761810933 1103 92-93 0.9009456419720756 1103 94-95 0.7682286038422177 1103 96-97 1.1208999225426979 1103 98-99 1.2026916768743057 1103 100-101 1.1658177795290534 1104 1 0.22813874922947974 1104 2 0.2143541948284593 1104 3 0.15334217999487265 1104 4 0.15247375104817706 1104 5 0.03843766333470455 1104 6 0.07663861429174545 1104 7 0.08269775626317255 1104 8 0.18255418399050427 1104 9 0.32950381722228883 1104 10-11 0.22659641431106792 1104 12-13 0.19626759913975178 1104 14-15 0.17992406185988585 1104 16-17 0.28514121053628827 1104 18-19 0.17894675102973423 1104 20-21 0.14756911402412953 1104 22-23 0.2134670848299507 1104 24-25 0.18226102013585432 1104 26-27 0.2971552177222776 1104 28-29 0.18091374494965606 1104 30-31 0.42888917193750586 1104 32-33 0.4241127739042909 1104 34-35 0.3888560374532801 1104 36-37 0.4075133911145983 1104 38-39 0.4533742604959201 1104 40-41 0.5804040587039196 1104 42-43 0.45625721008481435 1104 44-45 0.6097029686862427 1104 46-47 0.6632602011170619 1104 48-49 0.883622299991373 1104 50-51 0.8716707359907119 1104 52-53 1.0273366700796203 1104 54-55 0.9282469721350353 1104 56-57 0.9001365875628267 1104 58-59 1.030922745093413 1104 60-61 1.0018096205814757 1104 62-63 1.0448569104057768 1104 64-65 0.9895400705108699 1104 66-67 0.7937929355782671 1104 68-69 1.1402256438357838 1104 70-71 1.046247616967296 1104 72-73 1.123789394286181 1104 74-75 1.372843313234135 1104 76-77 1.3791572464349855 1104 78-79 1.568583134664788 1104 80-81 1.6317358480323136 1104 82-83 1.3362283295818038 1104 84-85 1.2640534167518815 1104 86-87 1.1265849003632198 1104 88-89 1.115647887953262 1104 90-91 1.1788189375662057 1104 92-93 1.4761054577343806 1104 94-95 1.4632260707672202 1104 96-97 1.4124873097628523 1104 98-99 1.652917236567859 1104 100-101 1.659957384833426 1105 1 0.15631118395928922 1105 2 0.2172268420658625 1105 3 0.17588316360143352 1105 4 0.07541318638514838 1105 5 0.12492066272753988 1105 6 0.15364225727899594 1105 7 0.18986988741071542 1105 8 0.1436121682042284 1105 9 -0.049431367962895933 1105 10-11 0.09802363046164686 1105 12-13 0.17183302111911303 1105 14-15 0.14388884631647159 1105 16-17 0.20985090998983935 1105 18-19 0.19849365449056933 1105 20-21 0.1919073046737907 1105 22-23 -0.040974703269625934 1105 24-25 0.10324614460458292 1105 26-27 0.2140932110434619 1105 28-29 0.18362511410570193 1105 30-31 0.30428762366792483 1105 32-33 0.18410252800264715 1105 34-35 0.0988230228813265 1105 36-37 0.24284847004597765 1105 38-39 0.30110688041092004 1105 40-41 0.39220202470270493 1105 42-43 0.32971083789294653 1105 44-45 0.41041448963341765 1105 46-47 0.5022515490223469 1105 48-49 0.5806889362998149 1105 50-51 0.6025848981036432 1105 52-53 0.4628273349247962 1105 54-55 0.2881285750494271 1105 56-57 0.408261208084987 1105 58-59 0.40907051376373005 1105 60-61 0.5285666029737044 1105 62-63 0.5356431884871355 1105 64-65 0.3945833309844602 1105 66-67 0.4350383818442083 1105 68-69 0.5375275564040862 1105 70-71 0.48893507901951594 1105 72-73 0.6084660791677869 1105 74-75 0.47288695318556506 1105 76-77 0.37743821182660753 1105 78-79 0.5965525487510028 1105 80-81 0.7806391570790652 1105 82-83 0.6309137424536893 1105 84-85 0.3345983469279581 1105 86-87 0.41970686454051176 1105 88-89 0.23160872584033143 1105 90-91 0.3020069308873943 1105 92-93 0.4747734905212653 1105 94-95 0.5523293646348577 1105 96-97 0.9451015325922398 1105 98-99 1.0657910222387734 1105 100-101 1.1717117108807855 1106 1 0.08581279869264336 1106 2 0.0898663855763786 1106 3 0.04697725455019963 1106 4 0.02321638530284531 1106 5 -0.02813532924243134 1106 6 0.030610486309285534 1106 7 -0.026232713619222636 1106 8 -0.027875786757803667 1106 9 0.07938016647020163 1106 10-11 0.05593500560348019 1106 12-13 -6.330476597966594E-4 1106 14-15 0.06278995480909089 1106 16-17 0.15559123250010032 1106 18-19 0.1833495286286535 1106 20-21 0.03433769657394237 1106 22-23 0.13108468182245758 1106 24-25 -0.021458184626453658 1106 26-27 0.07270775891812775 1106 28-29 0.10466165290294072 1106 30-31 0.05298110800064393 1106 32-33 0.3029777243891374 1106 34-35 0.03580178040947857 1106 36-37 0.14195354772775204 1106 38-39 0.2557255087603991 1106 40-41 0.4697992360145662 1106 42-43 0.38503318449328106 1106 44-45 0.15186704047570032 1106 46-47 0.27541369879977395 1106 48-49 0.5002899444171263 1106 50-51 0.2133438773303169 1106 52-53 0.34616530516133537 1106 54-55 0.3642414318182148 1106 56-57 0.3038430576092992 1106 58-59 0.5990556211216713 1106 60-61 0.5333557057056595 1106 62-63 0.5638290137468047 1106 64-65 0.36143383722517797 1106 66-67 0.2956852571169648 1106 68-69 0.4012751561247825 1106 70-71 0.3663642707800143 1106 72-73 0.38562087536225675 1106 74-75 0.3738926156626512 1106 76-77 0.18465377872893995 1106 78-79 0.44862688103694026 1106 80-81 0.7126408809160196 1106 82-83 0.6486755365533661 1106 84-85 0.5607646644630897 1106 86-87 0.6983419238928725 1106 88-89 0.582825231033663 1106 90-91 0.6461756159707477 1106 92-93 0.6447138108494492 1106 94-95 0.6660505067313949 1106 96-97 0.7224486238901378 1106 98-99 0.9359710977384701 1106 100-101 0.9055521032173495 1107 1 0.024191669486839373 1107 2 -0.05283034747662185 1107 3 -0.04963037514740165 1107 4 0.13070613689868793 1107 5 0.07091739475368541 1107 6 0.13055635625192252 1107 7 0.019550092826214893 1107 8 0.03944550153755699 1107 9 0.12303011663093599 1107 10-11 0.04182323363624363 1107 12-13 0.10221934231425678 1107 14-15 0.22712880896819598 1107 16-17 0.1611610173661262 1107 18-19 0.11357885523754163 1107 20-21 0.09879339244223218 1107 22-23 0.006098709428790983 1107 24-25 0.15603559371756148 1107 26-27 0.0703986498856608 1107 28-29 0.15172443716826223 1107 30-31 0.09795592432151778 1107 32-33 0.008656099431213704 1107 34-35 0.12131076797937368 1107 36-37 0.11782804520938583 1107 38-39 0.20576082532221562 1107 40-41 0.19277975579514361 1107 42-43 -0.028205245206962104 1107 44-45 0.20087025527300995 1107 46-47 0.47380266947052263 1107 48-49 0.5474781519860912 1107 50-51 0.48819122396731984 1107 52-53 0.6193603648034127 1107 54-55 0.7411397795312169 1107 56-57 0.648521478859962 1107 58-59 0.5775491552203036 1107 60-61 0.4405005432164657 1107 62-63 0.8091959429222442 1107 64-65 0.7279108286501881 1107 66-67 0.6315078029459826 1107 68-69 0.7515273229773811 1107 70-71 0.7721225206628368 1107 72-73 0.639266732762561 1107 74-75 0.5839776394600733 1107 76-77 0.693947315468062 1107 78-79 0.8882279689190717 1107 80-81 0.9733153942405934 1107 82-83 0.9806832335834734 1107 84-85 0.7570656671893978 1107 86-87 0.7523194929251993 1107 88-89 0.566634402777769 1107 90-91 0.5893172716903834 1107 92-93 0.5284091114362965 1107 94-95 0.825995914588173 1107 96-97 0.9776266259629196 1107 98-99 0.9903912790081506 1107 100-101 0.9199289144288691 1108 1 -0.09819789575754356 1108 2 -0.10621690985780674 1108 3 -0.11801495889779545 1108 4 0.06392695985390873 1108 5 -0.16457225817119792 1108 6 -0.04234881689767178 1108 7 0.035949450353157886 1108 8 0.004634790549559398 1108 9 -0.14654009372467414 1108 10-11 -0.09605435507236137 1108 12-13 -0.16366828697568536 1108 14-15 -0.13359653380049252 1108 16-17 -0.08227070435303574 1108 18-19 -0.06238854003081684 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661.0 10 1236.0 11 2092.0 12 3334.0 13 4565.0 14 5734.0 15 8069.0 16 10852.0 17 15288.0 18 13246.0 19 7062.0 20 5423.0 21 5480.0 22 5651.0 23 6166.0 24 7080.0 25 8410.0 26 10039.0 27 12382.0 28 15664.0 29 19263.0 30 24195.0 31 30303.0 32 38361.0 33 49780.0 34 67110.0 35 95349.0 36 146259.0 37 240365.0 38 204827.0 39 17198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.66621212975657 21.52443359308192 15.807622722654521 21.00173155450699 2 14.641087736162975 22.485580655895294 41.187932118164674 21.685399489777055 3 18.053229366549463 27.539274081139936 32.49590529114027 21.91159126117033 4 11.950786475523092 19.27643697336085 40.250596360927524 28.522180190188536 5 11.668547587291817 39.70785189621755 35.4321080082853 13.191492508205343 6 27.353118683449495 40.18895006280159 18.801936081938745 13.655995171810172 7 24.95750841744618 36.135266950652465 22.430657156931883 16.476567474969478 8 25.156680490101934 35.38201758074787 21.173423883458 18.2878780456922 9 26.43175144804104 17.070294340278323 22.313464494227706 34.18448971745293 10-11 23.19735410761207 28.319357399727725 29.163736079905433 19.319552412754774 12-13 24.744519071072542 26.701673507526486 29.157127818562095 19.396679602838873 14-15 21.99164678796908 27.30427300345016 28.73942100539844 21.964659203182315 16-17 19.486348151256312 30.684450145936427 29.247082657872898 20.58211904493437 18-19 19.56601909353317 28.737717630508985 32.719696248487665 18.976567027470175 20-21 20.79430150295364 27.560203868963036 32.70263163610434 18.942862991978977 22-23 20.3328345559387 27.684964310767583 32.553681725800594 19.428519407493123 24-25 20.03987363182792 28.050138735098223 32.85267460195689 19.05731303111696 26-27 20.00646071855391 28.02454518482369 32.884179373208624 19.084814723413775 28-29 19.59874981341016 28.306522164100162 32.88976942348245 19.204958599007227 30-31 20.133586022002405 27.925117870019413 32.66691319219747 19.274382915780716 32-33 19.95841861337767 27.89585151960605 32.732333340573184 19.41339652644309 34-35 20.192848032238388 28.071011331497125 32.42920124960719 19.3069393866573 36-37 20.62997453831339 27.611902702987507 32.30458999012314 19.45353276857596 38-39 19.803799960440184 28.55049329609042 32.15547376574305 19.490232977726347 40-41 20.194824190993888 28.222240810944832 32.1389668254462 19.443968172615083 42-43 20.13862764363508 28.261442234014332 31.799183097003304 19.800747025347285 44-45 20.60439890531087 28.25114076330629 31.46436391296833 19.68009641841451 46-47 20.80866355255934 28.523935309873394 30.922780736405663 19.744620401161598 48-49 20.493326013298063 28.75438324081622 30.749554975128518 20.002735770757198 50-51 20.39397948766769 28.97093123474665 30.332289061860955 20.3028002157247 52-53 20.80560174278085 29.283404300942095 29.917676341491145 19.99331761478591 54-55 20.8622797106171 28.950628837084636 29.80177132650774 20.385320125790525 56-57 20.9843156464597 29.422627244748007 29.560523859256726 20.032533249535568 58-59 20.959925395309202 28.95631531203052 29.777780242419034 20.30597905024125 60-61 20.852437986319515 29.156942972090956 30.04965900447236 19.940960037117172 62-63 20.58557540653534 28.902746219079216 30.324354873964687 20.187323500420757 64-65 20.49034673198461 28.954304398562698 30.096290048641844 20.459058820810853 66-67 20.68217999995379 29.210308025388816 29.44330840201576 20.664203572641636 68-69 20.75900686389089 28.93814180013891 29.702860485976228 20.59999084999397 70-71 20.67928420706273 28.962884023317525 29.838354130985927 20.519477638633816 72-73 20.939395325900733 28.67508646025278 29.827781991217865 20.557736222628623 74-75 20.558875778475713 29.40318248450443 29.360573128192236 20.677368608827624 76-77 20.511899793933157 29.369215490274097 29.36302285590433 20.755861859888412 78-79 20.6954337922757 29.267477699523464 28.94313234057834 21.093956167622498 80-81 20.82334391272281 28.98201257326859 28.884690816264285 21.309952697744315 82-83 20.68876435753746 29.234936312473252 29.217513630822157 20.858785699167132 84-85 20.532323081551368 29.170003544445827 28.95534968707673 21.34232368692608 86-87 20.16910679412447 29.01553542166715 29.117385827266517 21.697971956941863 88-89 20.514820664917387 28.728457099972367 29.233138842240397 21.523583392869853 90-91 20.26423025343516 28.707139642116765 29.641682371001863 21.386947733446213 92-93 20.160909076205375 28.806421575310022 29.584995991136598 21.447673357348005 94-95 20.022551457064427 28.776860726267827 29.54850873868961 21.652079077978133 96-97 20.213988110642013 28.862377723720307 29.25591695194718 21.66771721369051 98-99 19.89657801705215 29.04780609533492 28.921278218073432 22.1343376695395 100-101 19.751646998802183 28.878303240021886 28.966660751519452 22.403389009656475 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 544.0 1 384.5 2 252.0 3 257.5 4 228.0 5 237.5 6 373.5 7 737.5 8 1164.5 9 1408.5 10 1305.0 11 1175.0 12 1264.0 13 1379.0 14 1613.5 15 1827.0 16 1939.0 17 2101.5 18 2308.0 19 2555.5 20 2919.0 21 3395.5 22 3958.5 23 4829.0 24 5839.5 25 7629.5 26 11086.5 27 14778.0 28 16179.5 29 17662.0 30 20831.0 31 24038.5 32 27006.5 33 29869.0 34 34430.5 35 38959.0 36 43561.5 37 46180.0 38 43910.5 39 41420.5 40 40720.0 41 39866.5 42 38437.5 43 37746.0 44 37887.0 45 38395.5 46 37514.0 47 36303.0 48 36862.0 49 35154.5 50 30875.0 51 27662.5 52 25562.0 53 23460.5 54 21734.0 55 19556.0 56 16798.0 57 14636.5 58 13149.5 59 11959.0 60 9897.0 61 7678.0 62 5805.0 63 4040.0 64 2663.0 65 1799.0 66 1285.5 67 968.5 68 726.5 69 517.0 70 360.0 71 232.5 72 150.0 73 109.0 74 71.5 75 54.0 76 32.0 77 14.0 78 9.0 79 8.0 80 6.0 81 3.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02698758478676573 2 0.009149900321540437 3 0.008780207379255976 4 0.0 5 0.005730240605409162 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.242323557111552E-5 18-19 0.003881775893986852 20-21 8.780207379255976E-4 22-23 0.0 24-25 0.005637817369838047 26-27 0.005175701191982469 28-29 0.005591605752052488 30-31 0.025786082724341228 32-33 4.1590456007001986E-4 34-35 0.0036045061872735057 36-37 0.014556659602450695 38-39 0.006746896196691433 40-41 0.007440070463474799 42-43 0.008133244730258166 44-45 0.0025878505959912347 46-47 0.001940887946993426 48-49 0.001571195004708964 50-51 0.004112833982914641 52-53 0.0027726970671334656 54-55 0.0019870995647789835 56-57 8.318091201400397E-4 58-59 8.780207379255976E-4 60-61 0.0 62-63 4.621161778555776E-5 64-65 0.00845672605475707 66-67 6.007510312122508E-4 68-69 0.001155290444638944 70-71 0.004251468836271314 72-73 0.011414269593032766 74-75 0.0052681244275535844 76-77 0.004482526925199103 78-79 0.009935497823894917 80-81 9.242323557111552E-5 82-83 0.005175701191982469 84-85 4.1590456007001986E-4 86-87 0.0 88-89 9.242323557111553E-4 90-91 5.545394134266931E-4 92-93 1.3863485335667328E-4 94-95 8.318091201400397E-4 96-97 2.7726970671334655E-4 98-99 3.6969294228446207E-4 100-101 0.0010166555912822707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1081979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.8035992472364 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74803866274151 42.06068753884291 2 13.718494876190945 15.036457909400694 3 4.393682539522289 7.223688513466783 4 1.8823564850524992 4.126396417890148 5 0.9382799164966916 2.571055826270756 6 0.5229430658601869 1.7195497326313707 7 0.3191355322267525 1.224284306959591 8 0.22048053223580072 0.966650138437457 9 0.15692705556171951 0.7740150717647949 >10 0.9229740163448306 9.6450112120537 >50 0.10006461619703563 3.8028564792259854 >100 0.07072864867853558 7.0502253264662365 >500 0.004210037779504832 1.5308157114427445 >1k 0.0013472120894415462 1.0931761079182079 >5k 3.3680302236038654E-4 1.1751297072285807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7070 0.6534322754877867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5663 0.5233927830392272 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3654 0.3377145027768561 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1581 0.14612113543793365 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1304 0.12051989918473464 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1126 0.10406856325307608 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1101 0.10175798236379818 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.3863485335667328E-4 14-15 4.621161778555776E-5 0.0 0.0 0.0 2.310580889277888E-4 16-17 9.242323557111552E-5 0.0 0.0 0.0 2.7726970671334655E-4 18-19 9.242323557111552E-5 0.0 0.0 0.0 2.7726970671334655E-4 20-21 9.242323557111552E-5 0.0 0.0 2.310580889277888E-4 2.7726970671334655E-4 22-23 9.242323557111552E-5 0.0 0.0 4.159045600700198E-4 2.7726970671334655E-4 24-25 1.8484647114223104E-4 0.0 0.0 9.70443973496713E-4 2.7726970671334655E-4 26-27 1.8484647114223104E-4 0.0 0.0 0.0017098298580656372 4.621161778555776E-4 28-29 1.8484647114223104E-4 0.0 0.0 0.004020410747343525 4.621161778555776E-4 30-31 1.8484647114223104E-4 0.0 0.0 0.008133244730258166 4.621161778555776E-4 32-33 1.8484647114223104E-4 0.0 0.0 0.01238471356652948 4.621161778555776E-4 34-35 2.310580889277888E-4 0.0 0.0 0.016682394020586352 4.621161778555776E-4 36-37 3.6969294228446207E-4 0.0 0.0 0.022597481097137745 4.621161778555776E-4 38-39 3.6969294228446207E-4 0.0 0.0 0.03193222788982041 5.545394134266931E-4 40-41 3.6969294228446207E-4 0.0 0.0 0.04427072983856434 5.545394134266931E-4 42-43 3.6969294228446207E-4 0.0 0.0 0.06580534372663426 5.545394134266931E-4 44-45 3.6969294228446207E-4 0.0 0.0 0.08151729377372388 5.545394134266931E-4 46-47 3.6969294228446207E-4 0.0 0.0 0.11317225195683095 5.545394134266931E-4 48-49 3.6969294228446207E-4 0.0 0.0 0.15078850883427497 7.393858845689241E-4 50-51 3.6969294228446207E-4 0.0 0.0 0.16959663727299698 7.393858845689241E-4 52-53 3.6969294228446207E-4 0.0 0.0 0.20236067428295743 9.242323557111552E-4 54-55 3.6969294228446207E-4 0.0 0.0 0.2619736612263269 0.0011090788268533862 56-57 4.621161778555776E-4 0.0 0.0 0.353611299295088 0.0011090788268533862 58-59 4.621161778555776E-4 0.0 0.0 0.48254171291679415 0.0011090788268533862 60-61 4.621161778555776E-4 4.621161778555776E-5 0.0 0.6302802549772223 0.0012015020624245017 62-63 4.621161778555776E-4 9.242323557111552E-5 0.0 0.7827785936695629 0.0013863485335667328 64-65 4.621161778555776E-4 9.242323557111552E-5 0.0 0.9452124301857985 0.0013863485335667328 66-67 4.621161778555776E-4 9.242323557111552E-5 0.0 1.1522404778650972 0.0013863485335667328 68-69 5.545394134266931E-4 9.242323557111552E-5 0.0 1.4151383714471353 0.0013863485335667328 70-71 5.545394134266931E-4 9.242323557111552E-5 0.0 1.5619064695340668 0.0013863485335667328 72-73 5.545394134266931E-4 9.242323557111552E-5 0.0 1.7394515050661796 0.0013863485335667328 74-75 5.545394134266931E-4 9.242323557111552E-5 0.0 1.938577366104148 0.0013863485335667328 76-77 5.545394134266931E-4 9.242323557111552E-5 0.0 2.1461599531968734 0.0013863485335667328 78-79 5.545394134266931E-4 9.242323557111552E-5 0.0 2.3784657558048723 0.0013863485335667328 80-81 5.545394134266931E-4 9.242323557111552E-5 0.0 2.72417486845863 0.0013863485335667328 82-83 5.545394134266931E-4 9.242323557111552E-5 0.0 3.0858732008661907 0.0014787717691378483 84-85 6.007510312122509E-4 9.242323557111552E-5 0.0 3.6105599092034133 0.0014787717691378483 86-87 6.469626489978087E-4 9.242323557111552E-5 0.0 4.271894371332531 0.0014787717691378483 88-89 6.469626489978087E-4 9.242323557111552E-5 0.0 5.035033027443232 0.0014787717691378483 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4585 0.0 42.902214 1 GTATCAA 7480 0.0 36.20695 1 ATCAACG 9510 0.0 28.27185 3 TCAACGC 9600 0.0 28.103168 4 TATCAAC 9620 0.0 28.000544 2 CAACGCA 9795 0.0 27.495195 5 TAGACGG 70 0.008392811 27.141891 5 AACGCAG 9960 0.0 27.084888 6 GAGTACT 6390 0.0 24.304962 12-13 ACGCAGA 11120 0.0 24.088646 7 CGCAGAG 11145 0.0 23.94938 8 GTACATG 6225 0.0 23.356123 1 TACATGG 6225 0.0 22.666037 2 GCAGAGT 11865 0.0 22.295925 9 ACATGGG 6060 0.0 22.261993 3 GTACTTT 7020 0.0 22.022774 14-15 AGTACTT 6685 0.0 21.882524 12-13 GTATAGA 370 0.0 21.830612 1 AGAGTAC 9935 0.0 21.512093 10-11 CATGGGA 3495 0.0 19.97783 4 >>END_MODULE