FastQCFastQC Report
Thu 26 May 2016
SRR937101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176513
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67340.5723693660843526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.4086652676171024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.26978027442110714No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC13740.1167857898722751No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13310.11313092163027522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45150.039.2567331
GTATCAA75400.037.4980551
GTACCGT600.00394344831.6790266
TATACGA600.00394344831.6790265
TCAACGC90500.030.978944
ATCAACG91050.030.791813
CAACGCA91550.030.5717335
TATCAAC92100.030.5439472
AACGCAG93300.030.0492386
CATTCCG3050.026.4823679
CGCAGAG103950.026.3740928
ACGCAGA104400.026.3514357
GAGTACT53150.025.1173512-13
GCAGAGT109850.024.5239689
GTACTAG2751.8189894E-1224.1912571
GTACATG65850.023.0196111
TACATGG64700.022.914662
GTACTTT58600.022.65733314-15
AGTACTT57600.022.18710112-13
AGAGTAC89700.022.11422710-11
Produced by FastQC (version 0.11.5)