##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937101_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1176513 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.889896669225074 33.0 31.0 34.0 30.0 34.0 2 32.01100030343906 33.0 31.0 34.0 30.0 34.0 3 32.0024700109561 33.0 31.0 34.0 30.0 34.0 4 35.600115765826644 37.0 35.0 37.0 33.0 37.0 5 35.61280750828933 37.0 35.0 37.0 33.0 37.0 6 35.70220558548864 37.0 35.0 37.0 33.0 37.0 7 35.645225339626506 37.0 35.0 37.0 33.0 37.0 8 35.610924826160016 37.0 35.0 37.0 33.0 37.0 9 37.236077289413714 39.0 37.0 39.0 34.0 39.0 10-11 37.12592848527811 39.0 37.0 39.0 33.0 39.0 12-13 37.06271456413997 39.0 37.0 39.0 33.0 39.0 14-15 38.23235442362303 40.0 38.0 41.0 33.0 41.0 16-17 38.17386463218001 40.0 38.0 41.0 33.0 41.0 18-19 38.18312249843393 40.0 38.0 41.0 33.0 41.0 20-21 38.11230177652096 40.0 38.0 41.0 33.0 41.0 22-23 37.975632653442844 40.0 38.0 41.0 33.0 41.0 24-25 38.07211097539934 40.0 38.0 41.0 33.0 41.0 26-27 38.03337617178901 40.0 38.0 41.0 33.0 41.0 28-29 37.94356585945077 40.0 38.0 41.0 33.0 41.0 30-31 37.74478395053858 40.0 37.0 41.0 33.0 41.0 32-33 37.51551619064132 40.0 37.0 41.0 32.5 41.0 34-35 37.3209854034762 40.0 36.0 41.0 32.0 41.0 36-37 37.139630841308175 40.0 36.0 41.0 31.0 41.0 38-39 36.980924562669514 39.0 36.0 41.0 31.0 41.0 40-41 36.811972753382236 39.0 36.0 41.0 31.0 41.0 42-43 36.55482897341551 39.0 35.5 41.0 30.0 41.0 44-45 36.24960412677123 39.0 35.0 41.0 30.0 41.0 46-47 35.857685380442035 39.0 35.0 41.0 28.5 41.0 48-49 35.574815152913736 39.0 35.0 40.5 27.0 41.0 50-51 34.85865477049552 38.0 34.0 40.0 26.0 40.5 52-53 35.05379243578269 38.5 35.0 40.0 26.5 40.5 54-55 35.473564253008675 39.0 35.0 40.5 27.0 41.0 56-57 35.410098740940384 39.0 35.0 41.0 26.5 41.0 58-59 35.21078135133229 39.0 35.0 41.0 26.0 41.0 60-61 34.99271193773465 39.0 35.0 41.0 26.0 41.0 62-63 34.69329578168707 38.0 34.5 40.0 25.5 41.0 64-65 34.39316055156212 38.0 34.0 40.0 24.5 41.0 66-67 34.04027409811876 37.5 34.0 40.0 23.0 41.0 68-69 33.64042981250526 37.0 34.0 39.5 22.0 41.0 70-71 33.26159974432922 36.5 33.0 39.0 22.0 41.0 72-73 32.866185923997435 36.0 33.0 39.0 20.5 40.5 74-75 32.42912190515532 35.5 32.5 38.5 19.5 40.0 76-77 31.990420420343845 35.0 32.0 37.0 18.0 39.5 78-79 31.56058879077409 35.0 32.0 37.0 16.0 39.0 80-81 31.1141508848606 35.0 31.0 36.5 11.0 39.0 82-83 30.684769739050907 35.0 31.0 36.0 7.5 37.5 84-85 30.295929581738577 34.5 31.0 35.5 6.5 37.0 86-87 29.96159370954677 34.0 30.5 35.0 2.0 37.0 88-89 29.59968525634651 34.0 30.0 35.0 2.0 36.0 90-91 29.21408518222918 34.0 30.0 35.0 2.0 36.0 92-93 28.893832877324773 34.0 29.0 35.0 2.0 36.0 94-95 28.519373776575353 34.0 29.0 35.0 2.0 35.0 96-97 28.057607523248787 34.0 29.0 35.0 2.0 35.0 98-99 27.47880643902787 34.0 28.5 35.0 2.0 35.0 100-101 25.107235959143672 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33508371199824083 1101 2 0.3909208271854574 1101 3 0.3241219431612983 1101 4 0.33594922010692585 1101 5 0.30826061518571635 1101 6 0.13418941303260823 1101 7 0.13150984146925992 1101 8 0.17533589367745606 1101 9 0.34109861230624716 1101 10-11 0.355887963294947 1101 12-13 0.4489790805250138 1101 14-15 0.4162550905550617 1101 16-17 0.44313778332358567 1101 18-19 0.42908053871119023 1101 20-21 0.46559759648165056 1101 22-23 0.49283528862481063 1101 24-25 0.49801962346382567 1101 26-27 0.51616410179291 1101 28-29 0.385861246301765 1101 30-31 0.4416017239997174 1101 32-33 0.4989017133512661 1101 34-35 0.48188561364920446 1101 36-37 0.5820579287103698 1101 38-39 0.5947494260003268 1101 40-41 0.39607734453500854 1101 42-43 0.4916749992657756 1101 44-45 0.5236011003887739 1101 46-47 0.6129784543362646 1101 48-49 0.6095937657207031 1101 50-51 0.6998462929099887 1101 52-53 0.7601228748816666 1101 54-55 0.812549048723902 1101 56-57 0.7135875854449694 1101 58-59 0.7171727955878922 1101 60-61 0.7957104188817397 1101 62-63 0.8988314123073664 1101 64-65 0.9426573807504965 1101 66-67 0.9093707301201448 1101 68-69 0.8958841441560281 1101 70-71 0.6436675921005204 1101 72-73 0.5969413487138837 1101 74-75 0.6523052905805073 1101 76-77 0.8778739996318699 1101 78-79 0.7403662386599272 1101 80-81 0.5311533895578826 1101 82-83 0.4973842469966172 1101 84-85 0.5812555340621763 1101 86-87 0.5997433697535186 1101 88-89 0.6257045490556052 1101 90-91 0.5643158283202148 1101 92-93 0.5109371632233852 1101 94-95 0.666727635918857 1101 96-97 0.5098298985210725 1101 98-99 0.13870607434659377 1101 100-101 0.6011725330615683 1102 1 0.3669157082880723 1102 2 0.36436497231634846 1102 3 0.3216091894437234 1102 4 0.2078624257550672 1102 5 0.3147048195214879 1102 6 0.21644719107352017 1102 7 0.291896844024933 1102 8 0.38113698502071713 1102 9 0.4044840952700426 1102 10-11 0.4072161260688958 1102 12-13 0.45882090482466964 1102 14-15 0.49132823324868724 1102 16-17 0.5663700243269076 1102 18-19 0.6824035358594855 1102 20-21 0.6057282665821973 1102 22-23 0.5181825005684075 1102 24-25 0.20346967921508252 1102 26-27 0.4225587220429503 1102 28-29 0.34322127610155206 1102 30-31 0.24180980447888345 1102 32-33 0.4017521815631824 1102 34-35 0.32098747564263164 1102 36-37 0.22478941978523181 1102 38-39 0.30886978951827615 1102 40-41 0.33710052817867364 1102 42-43 0.47810104743880544 1102 44-45 0.2937101607086774 1102 46-47 0.3920640843255043 1102 48-49 0.34635407477016855 1102 50-51 0.4193451729510329 1102 52-53 0.5369824014399853 1102 54-55 0.6767489687973196 1102 56-57 0.6812694924341045 1102 58-59 0.600228482709845 1102 60-61 0.6379385451460067 1102 62-63 0.8448878740264902 1102 64-65 0.7766043479806015 1102 66-67 0.6999651717688025 1102 68-69 0.7791028913807949 1102 70-71 0.7015758837437573 1102 72-73 0.5880121429473206 1102 74-75 0.7475236332386075 1102 76-77 0.8965848380716217 1102 78-79 0.6848485906723987 1102 80-81 0.6148417054751114 1102 82-83 0.75232995118931 1102 84-85 0.625846428351867 1102 86-87 0.6383797333898826 1102 88-89 0.5353801063215258 1102 90-91 0.4501992884506656 1102 92-93 0.4064687983634556 1102 94-95 0.524485941869937 1102 96-97 0.4401562407450008 1102 98-99 0.20357266602041335 1102 100-101 0.5054867185504541 1103 1 0.16821742439273635 1103 2 0.31109851841037894 1103 3 0.23953345323399944 1103 4 0.2782280772728427 1103 5 0.2515394269450226 1103 6 0.265852203319632 1103 7 0.21554008227568033 1103 8 0.25368879389893806 1103 9 0.18401391887658036 1103 10-11 0.2690711436752551 1103 12-13 0.28142652236670784 1103 14-15 0.4019228705246931 1103 16-17 0.47672190412536253 1103 18-19 0.4964291454848464 1103 20-21 0.4446542992549851 1103 22-23 0.5117249455021451 1103 24-25 0.3393633565939993 1103 26-27 0.4175932750393301 1103 28-29 0.35239606560865866 1103 30-31 0.5627965234286023 1103 32-33 0.5701805150202119 1103 34-35 0.40985835958910144 1103 36-37 0.5367513331471017 1103 38-39 0.5472638173750823 1103 40-41 0.4674932489591299 1103 42-43 0.49800922223243305 1103 44-45 0.568900758830118 1103 46-47 0.7080271150933442 1103 48-49 0.8083725800025974 1103 50-51 0.6625335321372674 1103 52-53 0.7740353359707228 1103 54-55 0.8749302978094633 1103 56-57 0.8974272445926417 1103 58-59 0.9293174257510088 1103 60-61 1.031569537287055 1103 62-63 0.9591003708309529 1103 64-65 0.9126366702883146 1103 66-67 0.7196553287004335 1103 68-69 0.8230048853938072 1103 70-71 0.8474035475818553 1103 72-73 0.7510353858161309 1103 74-75 0.8456785733275041 1103 76-77 0.7414092074175862 1103 78-79 0.6538024347963294 1103 80-81 0.8911770262242307 1103 82-83 0.8606621506235967 1103 84-85 0.8596481650728798 1103 86-87 0.6727739839032267 1103 88-89 0.6665742875639467 1103 90-91 0.6250954851471029 1103 92-93 0.7663059910768162 1103 94-95 0.9309753491912112 1103 96-97 0.7181207682595776 1103 98-99 0.7264124852562865 1103 100-101 1.1390141547840926 1104 1 0.32334503939000925 1104 2 0.35163930964307255 1104 3 0.159447839356762 1104 4 0.28583552651497257 1104 5 0.2896392835209056 1104 6 0.28988096002850483 1104 7 0.35296664553475665 1104 8 0.3701716102522994 1104 9 0.3689985619453253 1104 10-11 0.4348325205003718 1104 12-13 0.3738894228266574 1104 14-15 0.5030483208265508 1104 16-17 0.5092921929562593 1104 18-19 0.29015002151980696 1104 20-21 0.5454242328300225 1104 22-23 0.4820122691061428 1104 24-25 0.33657777506545017 1104 26-27 0.30282456236641053 1104 28-29 0.46293176685055215 1104 30-31 0.4014762795264488 1104 32-33 0.4586633383302825 1104 34-35 0.3888308423335687 1104 36-37 0.4092909776825522 1104 38-39 0.43654593736204816 1104 40-41 0.5116052948455732 1104 42-43 0.5355881953528794 1104 44-45 0.5787218902921794 1104 46-47 0.44375050249961845 1104 48-49 0.6497222623709362 1104 50-51 0.3519851059183665 1104 52-53 0.46626843320113665 1104 54-55 0.4320829813013134 1104 56-57 0.6051651031094423 1104 58-59 0.7125228039813791 1104 60-61 0.6017018422644611 1104 62-63 0.7374292422095508 1104 64-65 0.6785601383923918 1104 66-67 0.5767460105389048 1104 68-69 0.4523250005968791 1104 70-71 0.49722261522076394 1104 72-73 0.5301841341305789 1104 74-75 0.606341031572768 1104 76-77 0.6598432642098224 1104 78-79 0.6549846402348471 1104 80-81 0.5784224542182521 1104 82-83 0.602060182107337 1104 84-85 0.5473214305628566 1104 86-87 0.3802628502729988 1104 88-89 0.5538486728519061 1104 90-91 0.3902974847366565 1104 92-93 0.3677168113073819 1104 94-95 0.2997598211249546 1104 96-97 0.23978091108077848 1104 98-99 0.10303528215406033 1104 100-101 0.43286633866841484 1105 1 0.20185484564244405 1105 2 0.3178314698855722 1105 3 0.13998672435115722 1105 4 0.2349839148072519 1105 5 0.2234521929159996 1105 6 0.201021401226825 1105 7 -0.003587259572114476 1105 8 0.15388618615963878 1105 9 0.29709012309651683 1105 10-11 0.3122735072965881 1105 12-13 0.34685601741123406 1105 14-15 0.21846106799323906 1105 16-17 0.3912121171564351 1105 18-19 0.3647656468421232 1105 20-21 0.35332779783414026 1105 22-23 0.46514418632070686 1105 24-25 0.25951771034890214 1105 26-27 0.1411190313917956 1105 28-29 0.2144910300399161 1105 30-31 -0.10170465583917121 1105 32-33 0.313968539254013 1105 34-35 -0.04670993940454338 1105 36-37 0.03357891211989994 1105 38-39 0.2661751720390768 1105 40-41 0.15357804816574827 1105 42-43 0.15757591284469896 1105 44-45 0.21324822006685906 1105 46-47 0.14060033458972043 1105 48-49 0.23936761499919612 1105 50-51 0.1633792038113242 1105 52-53 0.24440186256622098 1105 54-55 0.2719301579502158 1105 56-57 0.41295241607915045 1105 58-59 0.2387396481145032 1105 60-61 0.18178917988309706 1105 62-63 0.47300778400695975 1105 64-65 0.34078382129180085 1105 66-67 0.27133967908599743 1105 68-69 0.4471784443667346 1105 70-71 0.5918552675327788 1105 72-73 0.2722595039081952 1105 74-75 0.25746727778584244 1105 76-77 0.21904666681072626 1105 78-79 0.43976453929661474 1105 80-81 0.42462293565900566 1105 82-83 0.47193639554257416 1105 84-85 0.43925144159368656 1105 86-87 0.43334877363756164 1105 88-89 0.43070070761213586 1105 90-91 0.3332990248575918 1105 92-93 0.13068066129505596 1105 94-95 0.32405946817329223 1105 96-97 0.37162312997374514 1105 98-99 0.13307550710611338 1105 100-101 0.27650438052964077 1106 1 -0.07882670191217045 1106 2 9.378372024677617E-4 1106 3 0.044836363875720764 1106 4 0.11670900086670599 1106 5 0.11050971743482307 1106 6 0.0721595510027484 1106 7 0.04060075056016643 1106 8 0.024664943006506235 1106 9 0.05655882776645882 1106 10-11 -0.06676370935672793 1106 12-13 0.09349807504400331 1106 14-15 0.19535166965164308 1106 16-17 0.09670043688623764 1106 18-19 0.027360636991289766 1106 20-21 0.2486253795094271 1106 22-23 0.19271243850196385 1106 24-25 0.30666638211057773 1106 26-27 0.06529470092350209 1106 28-29 0.19559475603528398 1106 30-31 0.10829989069788581 1106 32-33 -0.06796655351700309 1106 34-35 -0.07196054019694742 1106 36-37 0.023524570177009707 1106 38-39 -0.2085958773449832 1106 40-41 -0.11441216595449788 1106 42-43 -0.0990923415015672 1106 44-45 -0.03570715891948595 1106 46-47 0.015756757114566255 1106 48-49 -0.2693043631774259 1106 50-51 -0.178210785147094 1106 52-53 -0.1676129028541169 1106 54-55 -0.1714048352299784 1106 56-57 0.04704061889800215 1106 58-59 -0.2750309466158498 1106 60-61 -0.009109085937765826 1106 62-63 0.026151389627351307 1106 64-65 0.04569083378395078 1106 66-67 -0.06425429350488088 1106 68-69 -0.1026700043981208 1106 70-71 -0.16405306562013777 1106 72-73 -0.1700308682583369 1106 74-75 -0.1082031199279001 1106 76-77 -0.17235280059493263 1106 78-79 -0.04502404673035798 1106 80-81 -0.061759103354610545 1106 82-83 -0.06079001117763738 1106 84-85 -0.09774654493989843 1106 86-87 0.04205106206121201 1106 88-89 0.11027268362374087 1106 90-91 0.007766971771360431 1106 92-93 -0.15684775456152877 1106 94-95 0.14155281074403092 1106 96-97 0.30733509602626796 1106 98-99 0.07316080880132603 1106 100-101 0.08389791578695238 1107 1 0.16545160521903313 1107 2 0.15014553886188153 1107 3 0.2462214572370378 1107 4 0.2040582044120427 1107 5 0.3648899582935172 1107 6 0.317312763317922 1107 7 0.18839218646453304 1107 8 0.2905010385602793 1107 9 0.42556619484217606 1107 10-11 0.10166852705470575 1107 12-13 0.19049201720882536 1107 14-15 0.28826150869817724 1107 16-17 0.3745067316316124 1107 18-19 0.1670945286568255 1107 20-21 0.32827707996350597 1107 22-23 0.4105025236025952 1107 24-25 0.2837021991909765 1107 26-27 0.18408178308354195 1107 28-29 0.21659403907022323 1107 30-31 0.2828278887028546 1107 32-33 0.2678801406506466 1107 34-35 0.4622449274409135 1107 36-37 0.39538525035863614 1107 38-39 0.19310509627278094 1107 40-41 0.2629961612309444 1107 42-43 0.39530701842527805 1107 44-45 0.46977138927823603 1107 46-47 0.6414293015092483 1107 48-49 0.5906432672011164 1107 50-51 0.5228028371889692 1107 52-53 0.5707914862189227 1107 54-55 0.672375233141473 1107 56-57 0.7914721902983715 1107 58-59 0.6517305533997373 1107 60-61 0.7080518363494548 1107 62-63 0.6209907998456643 1107 64-65 0.793053129734659 1107 66-67 0.8038968718231061 1107 68-69 0.6542315635732763 1107 70-71 0.6290495227276267 1107 72-73 0.5606541970478247 1107 74-75 0.6902995660933513 1107 76-77 0.7626989975326488 1107 78-79 0.7560404905154563 1107 80-81 0.8559868208549091 1107 82-83 0.7511666799766736 1107 84-85 0.7910324323977669 1107 86-87 0.8098136115473871 1107 88-89 0.8405234135772943 1107 90-91 0.7054129365049917 1107 92-93 0.6446999547870682 1107 94-95 0.8096831944762002 1107 96-97 0.7009083875193234 1107 98-99 0.7827265773566907 1107 100-101 0.7034975583435035 1108 1 -0.20182592491139317 1108 2 0.10371283997747227 1108 3 0.04645141549077181 1108 4 0.15434358850129115 1108 5 0.22246442938953237 1108 6 0.07085529969849347 1108 7 -0.04107313111371269 1108 8 0.14670401504557873 1108 9 0.12222649343412684 1108 10-11 0.024389581796562254 1108 12-13 -0.024603268057333594 1108 14-15 0.03872496302493289 1108 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64-65 -2.045861441101657 2316 66-67 -2.1307909804119376 2316 68-69 -2.0969062893751484 2316 70-71 -1.9450352521578793 2316 72-73 -2.432719962428912 2316 74-75 -2.4482316099563945 2316 76-77 -2.55474414039368 2316 78-79 -2.7086575325860665 2316 80-81 -2.885441319629418 2316 82-83 -2.3188818507509623 2316 84-85 -2.297631433713679 2316 86-87 -2.5075393338254806 2316 88-89 -2.533169813522459 2316 90-91 -2.1853129250122407 2316 92-93 -2.6419605156002213 2316 94-95 -2.3720053731845248 2316 96-97 -2.5709798674738806 2316 98-99 -2.742357143753665 2316 100-101 -2.702448933522863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1780.0 3 562.0 4 541.0 5 581.0 6 701.0 7 898.0 8 1118.0 9 1315.0 10 1824.0 11 2532.0 12 3884.0 13 5100.0 14 5682.0 15 7490.0 16 10078.0 17 14451.0 18 11982.0 19 6237.0 20 5970.0 21 6045.0 22 6413.0 23 7046.0 24 8033.0 25 9248.0 26 10928.0 27 12948.0 28 15534.0 29 19192.0 30 23680.0 31 29289.0 32 37782.0 33 49642.0 34 68264.0 35 97718.0 36 148607.0 37 239190.0 38 253967.0 39 50254.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43309970515921 20.684425128461488 15.24134776590933 21.64112740046998 2 14.30578778654376 21.543092390200197 42.036023737005806 22.115096086250237 3 17.75707369736434 27.22020801366923 32.249854424434595 22.772863864531843 4 11.395579768548275 18.517923395804214 41.07154395236298 29.014952883284533 5 11.736745824463766 38.973769323469156 35.91458680072152 13.374898051345552 6 27.231522921331308 39.661739332305515 19.534557811212487 13.572179935150691 7 24.975686061141904 35.40543067977012 22.896674261230316 16.722208997857653 8 24.743114062203688 34.99763162348171 21.797057770528554 18.462196543786053 9 26.085732918077 16.91974006517926 22.475536521556513 34.518990495187225 10-11 22.863260488741314 27.862301914046245 29.801513400279113 19.472924196933324 12-13 24.287903175749044 26.412650958404647 29.402484037926346 19.89696182791996 14-15 21.683264442785706 27.10748115640903 28.650632257099183 22.558622143706078 16-17 19.55347347292884 30.465038283843253 29.098526328138846 20.88296191508906 18-19 19.626438357270708 28.704363730804722 32.31297157991786 19.356226332006717 20-21 20.891827710831596 27.59857849577446 32.08304944194356 19.426544351450385 22-23 20.37793571497182 27.760819505328467 31.849633250794575 20.011611528905142 24-25 19.98635769610812 28.120528267406158 32.19094975509727 19.702164281388455 26-27 19.919210378130277 28.080577129439767 32.203482750927 19.796729741502958 28-29 19.634046105056836 28.352756979040144 32.2338028384504 19.77939407745262 30-31 20.096123034764595 27.95906207581217 32.052132020640656 19.89268286878258 32-33 19.887412580279946 27.927136917046035 32.138200505223935 20.04724999745008 34-35 20.301560628739335 28.166624593183414 31.709594369122996 19.82222040895426 36-37 20.540912000122397 27.690344263089315 31.699054749076293 20.06968898771199 38-39 19.704983884567262 28.50174923842681 31.611364779959743 20.181902097046184 40-41 20.204791617262195 28.225654965138506 31.584861365747763 19.98469205185153 42-43 20.003280884512865 28.201029823750208 31.35735122123701 20.438338070499917 44-45 20.518158981021433 28.18497736741205 30.959663377706043 20.337200273860475 46-47 20.59095019855301 28.43489909155198 30.540954958385463 20.433195751509547 48-49 20.273377852254583 28.48113074036754 30.695403796078807 20.550087611299073 50-51 20.162896696805177 28.782524622560324 30.24362257094591 20.81095610968859 52-53 20.57690313521563 29.096154205758406 29.807072807108504 20.51986985191746 54-55 20.609047397647075 28.672163795279697 29.77735524138265 20.941433565690573 56-57 20.66638504324648 29.22626373551651 29.506371375727575 20.600979845509436 58-59 20.69244104038701 28.858963140387477 29.70949112391835 20.73910469530716 60-61 20.51418376419269 28.805527647587816 30.065806137939592 20.6144824502799 62-63 20.314469578551094 28.64170425732837 30.23861676564762 20.805209398472915 64-65 20.290777112670817 28.738357972040006 30.069549363473513 20.901315551815657 66-67 20.378383685460328 28.959489492718664 29.507390565356893 21.154736256464115 68-69 20.4894032199862 28.61311243939521 29.82561206217594 21.07187227844265 70-71 20.50609559111081 28.640521653375956 29.913071939877238 20.940310815636 72-73 20.541491494618704 28.46902286619512 29.977638334381073 21.0118473048051 74-75 20.28139284399661 29.260996304598976 29.404196282156235 21.053414569248176 76-77 20.2411164815358 29.041400448054954 29.554665680435644 21.1628173899736 78-79 20.349922361799543 29.002299178901115 29.209688601317957 21.43808985798139 80-81 20.302356984814548 28.724423764066824 29.314988001639463 21.658231249479165 82-83 20.47484809205836 29.018423660440423 29.399251014030416 21.107477233470807 84-85 20.417438141726983 28.74456567016742 29.272314119141253 21.565682068964346 86-87 20.171460388569873 28.913900022527024 29.369115878150065 21.545523710753038 88-89 20.51874151798029 28.55849549318654 29.293586158618922 21.629176830214245 90-91 20.219745279786714 28.376794123122988 29.81689593639561 21.586564660694687 92-93 20.424782282288664 28.483547147160426 29.851961308610335 21.239709261940572 94-95 20.207517273271314 28.493757140525545 29.681294543278387 21.61743104292476 96-97 20.288686454528566 28.55919887341042 29.578969874440354 21.57314479762066 98-99 20.200269094325428 28.57050547647338 29.262966371711514 21.966259057489687 100-101 20.32472797261636 28.575449361219796 29.239080108891734 21.86074255727211 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 372.0 1 275.5 2 188.0 3 208.0 4 212.5 5 236.5 6 363.0 7 648.0 8 1107.0 9 1361.5 10 1235.0 11 1147.0 12 1285.5 13 1413.5 14 1474.0 15 1587.0 16 1749.0 17 1986.5 18 2218.0 19 2449.0 20 2715.0 21 3105.0 22 3681.5 23 4532.0 24 5638.0 25 7472.0 26 11131.5 27 15018.5 28 16559.0 29 18507.5 30 22123.5 31 25608.5 32 29027.5 33 32635.0 34 37723.0 35 42371.5 36 47297.5 37 51024.0 38 48842.0 39 45464.5 40 44480.0 41 43874.5 42 42597.5 43 41855.5 44 41901.0 45 42140.0 46 41029.5 47 39521.0 48 39964.0 49 38477.5 50 34078.0 51 30633.0 52 28380.5 53 26098.0 54 24072.5 55 21775.5 56 18959.5 57 16261.5 58 14766.0 59 13350.0 60 10945.5 61 8575.5 62 6386.0 63 4427.5 64 2955.0 65 2074.0 66 1427.0 67 1054.5 68 797.5 69 560.0 70 411.5 71 279.5 72 183.0 73 136.0 74 82.5 75 55.5 76 40.5 77 23.0 78 15.0 79 12.0 80 12.5 81 6.5 82 3.5 83 4.0 84 2.0 85 3.0 86 4.0 87 3.0 88 2.5 89 2.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024224126720231734 2 0.01036962617497639 3 0.012579546507348409 4 0.024224126720231734 5 0.05745792864167247 6 0.047258296338417 7 0.019804286055487702 8 0.051168155387998265 9 0.03161886014009195 10-11 0.02443661905988289 12-13 0.03773863952204523 14-15 0.021334230900976022 16-17 0.01984678452341793 18-19 0.018231842742069147 20-21 0.013047029654580952 22-23 0.008159705842604375 24-25 0.002167421864441787 26-27 4.2498467930231115E-5 28-29 0.0022099203323720177 30-31 0.0 32-33 4.249846793023111E-4 34-35 0.0 36-37 0.0 38-39 8.499693586046223E-5 40-41 0.0 42-43 8.499693586046223E-5 44-45 1.2749540379069336E-4 46-47 8.499693586046223E-5 48-49 8.074708906743912E-4 50-51 4.2498467930231115E-5 52-53 5.949785510232356E-4 54-55 0.0016999387172092445 56-57 8.499693586046223E-5 58-59 7.224739548139289E-4 60-61 0.00229491726823248 62-63 0.0020399264606510937 64-65 8.924678265348533E-4 66-67 0.009732149156022926 68-69 0.012027066424255405 70-71 0.021504224772696943 72-73 0.014406980628348349 74-75 0.015809430070045975 76-77 0.02702902560362699 78-79 0.01899681516481331 80-81 0.0441134097115799 82-83 0.03310630651765004 84-85 0.016574402492790137 86-87 0.012579546507348409 88-89 0.010837109322208934 90-91 0.02056925847823186 92-93 0.028388976577394382 94-95 0.010964604725999628 96-97 0.01865682742137146 98-99 0.029366441339789696 100-101 0.04101102155267303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1176513.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.32403153868151 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78595128267295 42.4810839049026 2 13.632408368108829 15.083995810108771 3 4.530321125700442 7.519068865158192 4 1.8731876705736665 4.145291750587475 5 0.9270730535275157 2.5644709426009027 6 0.5139255206614007 1.7059459028162787 7 0.33100792509192706 1.281888502113751 8 0.21431070039297653 0.9485225558094366 9 0.1505316469600193 0.7495215825587203 >10 0.8661101118388767 9.209938694021108 >50 0.10223870815174181 3.950464053904188 >100 0.06770234507889718 7.0638812869316805 >500 0.003538983780545774 1.257658270513677 >1k 0.001538688600237293 1.465026318907566 >5k 1.5386886002372933E-4 0.5732415590658657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6734 0.5723693660843526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4808 0.4086652676171024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3174 0.26978027442110714 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1374 0.1167857898722751 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1331 0.11313092163027522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.2749540379069336E-4 14-15 0.0 0.0 0.0 0.0 2.974892755116178E-4 16-17 0.0 0.0 0.0 1.2749540379069336E-4 6.799754868836978E-4 18-19 4.2498467930231115E-5 0.0 0.0 2.549908075813867E-4 6.799754868836978E-4 20-21 8.499693586046223E-5 0.0 0.0 3.399877434418489E-4 6.799754868836978E-4 22-23 8.499693586046223E-5 0.0 0.0 7.6497242274416E-4 7.6497242274416E-4 24-25 8.499693586046223E-5 0.0 0.0 0.001104960166186009 7.6497242274416E-4 26-27 8.499693586046223E-5 0.0 0.0 0.0015724433134185513 7.6497242274416E-4 28-29 1.2749540379069336E-4 0.0 0.0 0.0029748927551161777 7.6497242274416E-4 30-31 1.6999387172092446E-4 0.0 0.0 0.006544764061255592 7.6497242274416E-4 32-33 1.6999387172092446E-4 0.0 0.0 0.01032712770704616 7.6497242274416E-4 34-35 1.6999387172092446E-4 0.0 0.0 0.01372700514146465 8.499693586046223E-4 36-37 2.124923396511556E-4 0.0 0.0 0.019549295247906315 8.499693586046223E-4 38-39 2.549908075813867E-4 0.0 0.0 0.02783649649430138 8.499693586046223E-4 40-41 2.549908075813867E-4 0.0 0.0 0.03812112573341731 8.499693586046223E-4 42-43 2.549908075813867E-4 0.0 0.0 0.05609797766790507 8.499693586046223E-4 44-45 2.549908075813867E-4 0.0 0.0 0.07071745063590457 9.349662944650847E-4 46-47 3.399877434418489E-4 0.0 0.0 0.10046637818706636 0.0010199632303255469 48-49 4.249846793023112E-4 0.0 0.0 0.13659007592776282 0.0010199632303255469 50-51 4.249846793023112E-4 0.0 0.0 0.15261199833745995 0.0010199632303255469 52-53 4.249846793023112E-4 0.0 0.0 0.18805572059127268 0.0010199632303255469 54-55 4.249846793023112E-4 0.0 0.0 0.24589613544431724 0.0010199632303255469 56-57 5.099816151627734E-4 0.0 0.0 0.33671536141122116 0.001062461698255778 58-59 5.099816151627734E-4 0.0 0.0 0.4530761666041939 0.001104960166186009 60-61 5.099816151627734E-4 0.0 0.0 0.5870313375202825 0.001104960166186009 62-63 5.099816151627734E-4 0.0 0.0 0.7244713828066498 0.0011899571020464713 64-65 5.099816151627734E-4 0.0 0.0 0.8778058550989236 0.0011899571020464713 66-67 5.099816151627734E-4 0.0 0.0 1.062291704384057 0.0011899571020464713 68-69 5.099816151627734E-4 0.0 0.0 1.2992631615630257 0.0011899571020464713 70-71 5.099816151627734E-4 0.0 0.0 1.4360657298304396 0.0011899571020464713 72-73 5.099816151627734E-4 0.0 0.0 1.6070795647816896 0.0012324555699767025 74-75 5.099816151627734E-4 0.0 0.0 1.7984926643394505 0.0013599509737673957 76-77 5.099816151627734E-4 0.0 0.0 2.001762836449746 0.0014449479096278579 78-79 5.099816151627734E-4 0.0 0.0 2.2240298237248544 0.0014449479096278579 80-81 5.099816151627734E-4 0.0 0.0 2.540600911337146 0.0014449479096278579 82-83 5.099816151627734E-4 0.0 0.0 2.8718339703853677 0.0014449479096278579 84-85 5.524800830930045E-4 0.0 0.0 3.3548715568803744 0.0014449479096278579 86-87 5.949785510232356E-4 0.0 0.0 3.9684219383891213 0.0016149417813487825 88-89 5.949785510232356E-4 0.0 0.0 4.671941576506168 0.0016999387172092447 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4515 0.0 39.256733 1 GTATCAA 7540 0.0 37.498055 1 GTACCGT 60 0.003943448 31.679026 6 TATACGA 60 0.003943448 31.679026 5 TCAACGC 9050 0.0 30.97894 4 ATCAACG 9105 0.0 30.79181 3 CAACGCA 9155 0.0 30.571733 5 TATCAAC 9210 0.0 30.543947 2 AACGCAG 9330 0.0 30.049238 6 CATTCCG 305 0.0 26.482367 9 CGCAGAG 10395 0.0 26.374092 8 ACGCAGA 10440 0.0 26.351435 7 GAGTACT 5315 0.0 25.11735 12-13 GCAGAGT 10985 0.0 24.523968 9 GTACTAG 275 1.8189894E-12 24.191257 1 GTACATG 6585 0.0 23.019611 1 TACATGG 6470 0.0 22.91466 2 GTACTTT 5860 0.0 22.657333 14-15 AGTACTT 5760 0.0 22.187101 12-13 AGAGTAC 8970 0.0 22.114227 10-11 >>END_MODULE