FastQCFastQC Report
Thu 26 May 2016
SRR937101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176513
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75290.6399419300934202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59650.5070067224076572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38310.3256232612814308No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16420.139564968682879No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.11321591856613569No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC12540.10658615756901965No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12460.10590618208213595No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT12410.10548119740283361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.038.667021
TATCCCG756.912289E-638.008115
GTATCAA84200.033.0018121
ATCAACG105900.026.1912063
TCAACGC106000.026.1664984
TATCAAC107700.025.8416672
CAACGCA107650.025.730065
AACGCAG110500.025.109436
CATTCCG2850.025.0053379
GTACATG64100.023.194251
TACATGG64350.022.5107482
ACGCAGA123250.022.3577127
GAGTACT68500.022.2216412-13
CGCAGAG123450.022.1675518
AGTACTT72300.021.87482512-13
ACATGGG62450.021.82673
AGAGTAC104750.021.61201110-11
GCAGAGT128850.020.8329289
TACTCCG1150.003718728420.6565827
GTACTTT75200.019.98915714-15
Produced by FastQC (version 0.11.5)