##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937101_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1176513 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12376488827578 33.0 31.0 34.0 31.0 34.0 2 32.25067126330096 33.0 31.0 34.0 30.0 34.0 3 32.34277989278487 34.0 31.0 34.0 30.0 34.0 4 36.02234314452964 37.0 35.0 37.0 35.0 37.0 5 35.9318222578076 37.0 35.0 37.0 35.0 37.0 6 35.97457911642285 37.0 35.0 37.0 35.0 37.0 7 35.918927372668215 37.0 35.0 37.0 35.0 37.0 8 35.89127531952473 37.0 35.0 37.0 35.0 37.0 9 37.545303791798304 39.0 37.0 39.0 35.0 39.0 10-11 37.472836679237716 39.0 37.0 39.0 35.0 39.0 12-13 37.39394124841799 39.0 37.0 39.0 34.0 39.0 14-15 38.565787203371315 40.0 38.0 41.0 34.0 41.0 16-17 38.49462181888343 40.0 38.0 41.0 34.0 41.0 18-19 38.67792068595927 40.0 38.0 41.0 34.0 41.0 20-21 38.71974002837197 40.0 38.0 41.0 34.0 41.0 22-23 38.631229744167726 40.0 38.0 41.0 34.0 41.0 24-25 38.52014469878361 40.0 38.0 41.0 34.0 41.0 26-27 38.330514409955526 40.0 38.0 41.0 34.0 41.0 28-29 38.24427354393874 40.0 38.0 41.0 34.0 41.0 30-31 38.18627970961647 40.0 37.5 41.0 34.0 41.0 32-33 37.97282180477394 40.0 37.0 41.0 33.0 41.0 34-35 37.799945261973306 40.0 37.0 41.0 33.0 41.0 36-37 37.619106631205945 40.0 37.0 41.0 33.0 41.0 38-39 37.4414031124178 40.0 37.0 41.0 33.0 41.0 40-41 37.25533589514098 40.0 36.0 41.0 32.5 41.0 42-43 36.97031227024266 40.0 36.0 41.0 31.5 41.0 44-45 36.619569439521705 40.0 36.0 41.0 30.5 41.0 46-47 36.167084001621745 39.0 36.0 41.0 30.0 41.0 48-49 35.72733450459111 39.0 35.0 41.0 28.5 41.0 50-51 35.426386703759334 39.0 35.0 40.0 27.0 41.0 52-53 35.21365807262648 39.0 35.0 40.0 26.5 41.0 54-55 34.933920832154 38.5 35.0 40.0 25.5 41.0 56-57 34.66864454536414 38.0 34.0 40.0 24.5 41.0 58-59 34.359361095032526 38.0 34.0 40.0 23.5 41.0 60-61 34.054653454742954 38.0 34.0 40.0 23.0 41.0 62-63 33.72368728607334 37.5 33.0 40.0 22.0 41.0 64-65 33.368704808191666 37.0 33.0 39.5 21.5 41.0 66-67 32.99092572712753 36.0 32.0 39.0 20.0 40.0 68-69 32.68170049969699 36.0 32.0 39.0 19.5 40.0 70-71 32.24200880058274 35.5 31.5 38.5 18.0 40.0 72-73 31.729792615976194 35.0 31.0 38.0 13.5 40.0 74-75 31.225280978620724 35.0 31.0 37.0 11.5 39.0 76-77 29.770065014156238 33.5 29.0 35.5 9.0 38.5 78-79 30.4958215506331 34.0 30.0 36.0 7.5 39.0 80-81 30.198693087114208 34.0 30.0 36.0 7.0 37.5 82-83 29.903235238369657 34.0 30.0 35.5 4.0 37.0 84-85 29.56083528188809 34.0 30.0 35.0 2.0 37.0 86-87 29.227322180035408 34.0 29.0 35.0 2.0 36.0 88-89 28.834131029576383 34.0 29.0 35.0 2.0 36.0 90-91 28.40679660998221 34.0 29.0 35.0 2.0 35.5 92-93 27.942186359181754 33.5 28.5 35.0 2.0 35.0 94-95 27.378249114119434 33.0 26.5 35.0 2.0 35.0 96-97 26.709524246650908 33.0 25.5 34.0 2.0 35.0 98-99 25.9535606491386 32.0 24.5 34.0 2.0 35.0 100-101 22.62680310374811 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18069857809598489 1101 2 0.18207333369269207 1101 3 0.17909864992560642 1101 4 0.14915514142519726 1101 5 0.18051270490951055 1101 6 0.2320533785187564 1101 7 0.17680232895207837 1101 8 0.2389558705487147 1101 9 0.2794634920198007 1101 10-11 0.3853313468744588 1101 12-13 0.34017973664185064 1101 14-15 0.40150357494183453 1101 16-17 0.4728555850692189 1101 18-19 0.32347260787228294 1101 20-21 0.3219016995098727 1101 22-23 0.3383007235630515 1101 24-25 0.39474927151129435 1101 26-27 0.33698033065630995 1101 28-29 0.3578887059135596 1101 30-31 0.47989883654179977 1101 32-33 0.6271653668696615 1101 34-35 0.6839759532895187 1101 36-37 0.5775048193204455 1101 38-39 0.7074003342339594 1101 40-41 0.7992862459608361 1101 42-43 0.7826606742601641 1101 44-45 0.8923029843876478 1101 46-47 0.86076115146777 1101 48-49 0.9288340615420054 1101 50-51 0.8706022283003847 1101 52-53 0.9097538495612554 1101 54-55 0.9419062504128206 1101 56-57 1.0783091702958814 1101 58-59 0.9576808839473827 1101 60-61 1.0262747769119045 1101 62-63 1.0919376238235472 1101 64-65 1.1289205414087604 1101 66-67 0.9244148515951096 1101 68-69 0.8361124955578774 1101 70-71 0.857408638136075 1101 72-73 1.0401189330666263 1101 74-75 1.2463579697082956 1101 76-77 1.0678153684604297 1101 78-79 1.0281315833452211 1101 80-81 1.2283345593760764 1101 82-83 1.2560185617707234 1101 84-85 1.1201484383386102 1101 86-87 0.8544657006325345 1101 88-89 0.9767576299703755 1101 90-91 1.181624689469789 1101 92-93 1.1568416253597427 1101 94-95 1.1344501554660944 1101 96-97 1.0940402917348457 1101 98-99 1.3251013678998795 1101 100-101 1.2932742426335402 1102 1 0.3319806104021126 1102 2 0.2889810628895759 1102 3 0.36198671323536047 1102 4 0.17384234231722218 1102 5 0.17682917471995552 1102 6 0.12622219136226676 1102 7 0.06940813300366955 1102 8 0.2592379817946835 1102 9 0.28328072655992287 1102 10-11 0.3541000334142339 1102 12-13 0.3539766935110933 1102 14-15 0.4356608428340323 1102 16-17 0.43749362124339797 1102 18-19 0.3949996250318293 1102 20-21 0.40664731588199743 1102 22-23 0.4564243236143639 1102 24-25 0.39686288567309447 1102 26-27 0.3993782022409249 1102 28-29 0.5178791591269061 1102 30-31 0.5629743819667468 1102 32-33 0.5932090255338025 1102 34-35 0.5769028204633742 1102 36-37 0.5911599311683275 1102 38-39 0.6600153397104194 1102 40-41 0.4866497047098406 1102 42-43 0.6772656297084936 1102 44-45 0.7647090706491539 1102 46-47 0.8811958386193339 1102 48-49 0.9516889947885048 1102 50-51 1.0866371295762463 1102 52-53 1.0961272300611427 1102 54-55 1.0631309526616164 1102 56-57 0.9525295160523726 1102 58-59 0.9940870738174326 1102 60-61 1.0501599983874925 1102 62-63 1.1431299854074766 1102 64-65 1.12938776657478 1102 66-67 1.0820350128297278 1102 68-69 0.9212176582232843 1102 70-71 1.0153936494343405 1102 72-73 1.175676889708921 1102 74-75 1.23461431203091 1102 76-77 1.0516874661879498 1102 78-79 1.011239074735844 1102 80-81 1.2858539489737808 1102 82-83 1.2104308053395947 1102 84-85 1.2620094943562918 1102 86-87 1.067348065472732 1102 88-89 1.1774653749552293 1102 90-91 1.069071405079157 1102 92-93 1.271199548452607 1102 94-95 1.1395150094767281 1102 96-97 1.2355464885121243 1102 98-99 1.435325433696235 1102 100-101 1.5775822362788823 1103 1 0.12574395948633565 1103 2 0.1406299582657411 1103 3 0.2027512085884311 1103 4 0.1560720826337203 1103 5 0.08574203354746146 1103 6 0.09783142088817698 1103 7 0.021868900096677635 1103 8 0.01969665426690881 1103 9 0.20295737978473483 1103 10-11 0.2256086551702552 1103 12-13 0.1798252623243357 1103 14-15 0.3149758441122472 1103 16-17 0.22310784404837847 1103 18-19 0.23819633383831018 1103 20-21 0.1823280676364476 1103 22-23 0.14965515247394734 1103 24-25 0.0560870513295626 1103 26-27 0.25700326099751436 1103 28-29 0.2292652579369303 1103 30-31 0.32771919283238304 1103 32-33 0.4101178311876268 1103 34-35 0.4220591129845843 1103 36-37 0.3143844514259584 1103 38-39 0.3037602371647097 1103 40-41 0.42702413879113266 1103 42-43 0.5868918141175925 1103 44-45 0.5226628570473153 1103 46-47 0.5818336052917701 1103 48-49 0.7303699403366153 1103 50-51 0.7069366026224344 1103 52-53 0.6612073657991644 1103 54-55 0.6980989560921742 1103 56-57 0.6416344986109479 1103 58-59 0.7590375327373025 1103 60-61 0.6661248846264911 1103 62-63 0.7443639768145829 1103 64-65 0.7493120221185166 1103 66-67 0.7706817970920312 1103 68-69 0.6532304114950946 1103 70-71 0.5679756406435352 1103 72-73 0.7796100762538707 1103 74-75 0.901571153771588 1103 76-77 0.8395009377332698 1103 78-79 1.0429763936212417 1103 80-81 1.1076065651881208 1103 82-83 1.1989052369469277 1103 84-85 1.034707060802777 1103 86-87 0.8105132312693861 1103 88-89 0.5807695567749107 1103 90-91 0.4941583029787644 1103 92-93 0.8010107851859871 1103 94-95 0.737295762880489 1103 96-97 0.8652258837109947 1103 98-99 0.5332907033992775 1103 100-101 1.0554299721917353 1104 1 0.1239891300821867 1104 2 0.21530238431304127 1104 3 0.1890212772395401 1104 4 0.035537853894865634 1104 5 0.1016566608360776 1104 6 0.07627026621390343 1104 7 0.09645987469658479 1104 8 0.13104310176638023 1104 9 0.1615487393224342 1104 10-11 0.25373215875353594 1104 12-13 0.2297199261820424 1104 14-15 0.1685370115404865 1104 16-17 0.2826210252694423 1104 18-19 0.19580462368255525 1104 20-21 0.20630812739456417 1104 22-23 0.11881867799404944 1104 24-25 0.16942688531933925 1104 26-27 0.20284630956576422 1104 28-29 0.13538347906051484 1104 30-31 0.3096026227673221 1104 32-33 0.20931928815401335 1104 34-35 0.19044351627881895 1104 36-37 0.19068564554475387 1104 38-39 0.19503443925937347 1104 40-41 0.2863740113094764 1104 42-43 0.2778809651326313 1104 44-45 0.03213847204921905 1104 46-47 0.33515949108350185 1104 48-49 0.21437284708078863 1104 50-51 0.35057231696612234 1104 52-53 0.32361836342576566 1104 54-55 0.33942330445160707 1104 56-57 0.34537007648722096 1104 58-59 0.47328208650510817 1104 60-61 0.5720635357441424 1104 62-63 0.6459794691697454 1104 64-65 0.5558270074456573 1104 66-67 0.5115361908903608 1104 68-69 0.5737168418578733 1104 70-71 0.5738797111288925 1104 72-73 0.6538873033567363 1104 74-75 0.5306560453107245 1104 76-77 0.4561392654799832 1104 78-79 0.6257630940202112 1104 80-81 1.0368943896750373 1104 82-83 0.9730283439978962 1104 84-85 0.8685040779986011 1104 86-87 0.7908934327124406 1104 88-89 0.6638453953190115 1104 90-91 0.7954754685379619 1104 92-93 0.7879784086704404 1104 94-95 0.7005282606746341 1104 96-97 0.4457258564638913 1104 98-99 0.41257675972309826 1104 100-101 0.5081398705426459 1105 1 0.22721883885773053 1105 2 0.2347809808275798 1105 3 0.16339513329329947 1105 4 0.13650195911257157 1105 5 0.1315993389682788 1105 6 0.09222179348593329 1105 7 0.15465127274529777 1105 8 0.18398899824438075 1105 9 0.2023470848724287 1105 10-11 0.1977243759857572 1105 12-13 0.17931986185008952 1105 14-15 0.21066674881088687 1105 16-17 0.2684314592828656 1105 18-19 0.1359409444892279 1105 20-21 0.18338305139370448 1105 22-23 0.25154039160148045 1105 24-25 0.15973356343057077 1105 26-27 0.4077853602848691 1105 28-29 0.25114830381712494 1105 30-31 0.3588796749653582 1105 32-33 0.42862359402447936 1105 34-35 0.4966382637846465 1105 36-37 0.4775755627348488 1105 38-39 0.5849122959564497 1105 40-41 0.5401569224352301 1105 42-43 0.6087718403094087 1105 44-45 0.69482768753155 1105 46-47 0.7809998534402212 1105 48-49 0.7157640255617537 1105 50-51 0.7956499610880527 1105 52-53 0.7734434609288812 1105 54-55 0.7451596867931798 1105 56-57 0.8308357751444717 1105 58-59 0.7896972233940645 1105 60-61 0.7037365336306252 1105 62-63 0.7656157488948665 1105 64-65 0.634614245244876 1105 66-67 0.772464843607942 1105 68-69 0.7803400611476761 1105 70-71 0.5464730754977332 1105 72-73 0.8135901526678744 1105 74-75 0.8065104103560969 1105 76-77 0.4503303348763268 1105 78-79 0.7342162776652081 1105 80-81 0.7822912461501019 1105 82-83 0.8202558640885229 1105 84-85 0.9910553993384497 1105 86-87 0.9489203297620854 1105 88-89 0.5858241972412799 1105 90-91 0.5916314595384797 1105 92-93 0.7643427093314124 1105 94-95 0.8293376125826519 1105 96-97 1.062284866171371 1105 98-99 1.1099075081239747 1105 100-101 1.0632136162880848 1106 1 0.06788436528177044 1106 2 0.018777170396525378 1106 3 0.10618237700933264 1106 4 0.05673404900410617 1106 5 0.15006477446158328 1106 6 0.08735483382021414 1106 7 0.038435290585042026 1106 8 0.10179843339127359 1106 9 0.16249127504757865 1106 10-11 -0.0021662906231725287 1106 12-13 0.0942054406675581 1106 14-15 0.11350508694334138 1106 16-17 0.017922680136315705 1106 18-19 -0.030723196323528157 1106 20-21 -0.015624288016113042 1106 22-23 -0.002277617015288058 1106 24-25 0.05316922993124962 1106 26-27 0.05165619533217125 1106 28-29 -0.03656413853928342 1106 30-31 0.04706950371247132 1106 32-33 0.039015678719970026 1106 34-35 0.0014868208003804284 1106 36-37 0.09913589095152275 1106 38-39 0.04865907549270787 1106 40-41 0.12468787136246107 1106 42-43 0.18255672415622115 1106 44-45 -0.17274143065676384 1106 46-47 -0.038473397766772166 1106 48-49 0.0997374824454269 1106 50-51 -0.01382415612600596 1106 52-53 -0.02588213607472767 1106 54-55 -0.053179744673180096 1106 56-57 0.0728187148054289 1106 58-59 0.11488179114829222 1106 60-61 0.10593835657548567 1106 62-63 0.3248614567473851 1106 64-65 -0.06897210648389063 1106 66-67 0.3320504592307074 1106 68-69 0.2990306584760347 1106 70-71 -0.07846359773615674 1106 72-73 0.03546007840776966 1106 74-75 -0.010691737341407048 1106 76-77 -0.03133413068906776 1106 78-79 -0.1739285187148809 1106 80-81 -0.03173915069763211 1106 82-83 0.06527957103173421 1106 84-85 0.008524926715097081 1106 86-87 -0.03390133773450188 1106 88-89 -9.875977748521336E-4 1106 90-91 0.06609832394342519 1106 92-93 0.0830181515362689 1106 94-95 -0.08600911499317831 1106 96-97 -0.18858114088658695 1106 98-99 -0.519521576723065 1106 100-101 0.08507163443092836 1107 1 0.058040123342429695 1107 2 0.07710383630119821 1107 3 0.19132561415851512 1107 4 0.10305622608408527 1107 5 0.11711035889200616 1107 6 0.024858340714501992 1107 7 0.13310273309795662 1107 8 0.11154915814942967 1107 9 0.039806085051843354 1107 10-11 0.08546606216518882 1107 12-13 0.21491897675999638 1107 14-15 0.12180213907397075 1107 16-17 0.11499299466576218 1107 18-19 0.17535022505450826 1107 20-21 0.03883804014606085 1107 22-23 0.11486044551653407 1107 24-25 0.21810028698711648 1107 26-27 0.1492808589300907 1107 28-29 0.17494315060545063 1107 30-31 0.036262034806931354 1107 32-33 0.19307692208284521 1107 34-35 0.06639086178168441 1107 36-37 0.21439581676122543 1107 38-39 0.37919555238877223 1107 40-41 0.22723985132900282 1107 42-43 0.1458740902967648 1107 44-45 0.031680373393569994 1107 46-47 0.07995260416591066 1107 48-49 0.07056531731486615 1107 50-51 0.15957747938558953 1107 52-53 0.09430978196265016 1107 54-55 0.15087942053011005 1107 56-57 0.18797836941530477 1107 58-59 0.16447031555107827 1107 60-61 0.2707317355590604 1107 62-63 0.416662078117092 1107 64-65 0.2664038845384624 1107 66-67 0.15824773891981891 1107 68-69 0.40829561343193177 1107 70-71 0.3686051581736116 1107 72-73 0.34878835488612125 1107 74-75 0.2432733911408107 1107 76-77 0.24646956635682926 1107 78-79 0.5112812195409191 1107 80-81 0.5581433293780123 1107 82-83 0.511510309090113 1107 84-85 0.4071330793351393 1107 86-87 0.2788668747365328 1107 88-89 0.4143154498461712 1107 90-91 0.31641628235945163 1107 92-93 0.2096315826816486 1107 94-95 0.19432526088354152 1107 96-97 0.045076212161554 1107 98-99 -0.0181515500187146 1107 100-101 -0.009875320545742028 1108 1 0.0970551944526008 1108 2 0.01590781752717163 1108 3 -0.12877155794460293 1108 4 -0.017269724999664504 1108 5 0.06700894140574576 1108 6 0.05227879874417596 1108 7 0.1000736522234007 1108 8 0.03523721683006187 1108 9 0.1482166607729667 1108 10-11 0.09997878652189485 1108 12-13 -0.029767808305692256 1108 14-15 0.09206264050089175 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16.0 6 66.0 7 170.0 8 383.0 9 747.0 10 1367.0 11 2287.0 12 3775.0 13 5050.0 14 6252.0 15 8787.0 16 11913.0 17 16532.0 18 14208.0 19 7835.0 20 6030.0 21 5962.0 22 6339.0 23 6955.0 24 8050.0 25 9688.0 26 11528.0 27 14335.0 28 17931.0 29 22458.0 30 28217.0 31 34783.0 32 43919.0 33 57499.0 34 76469.0 35 108091.0 36 165923.0 37 262838.0 38 197452.0 39 12651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.70333855921972 21.452547024475514 15.816411550455879 21.027702865848887 2 14.744943235717129 22.487003206272462 41.07955839801979 21.688495159990616 3 18.094843061524234 27.53529075776533 32.3966946363488 21.973171544361637 4 11.951279632132323 19.35291667729131 40.19770337693687 28.49810031363949 5 11.736206909740904 39.67988454016233 35.413548341582285 13.170360208514484 6 27.405732023360557 40.15450743000714 18.757633787301966 13.682126759330327 7 25.038567359646684 36.071594619013986 22.475314764902727 16.414523256436606 8 25.15237825676384 35.27993315840964 21.311366725229554 18.256321859596962 9 26.48685266278627 17.025580699559377 22.245756949355382 34.24180968829897 10-11 23.17887634699501 28.36190360418525 29.130200273128697 19.329019775691037 12-13 24.779199209868487 26.63616976607993 29.17481574789229 19.409815276159296 14-15 22.03324570149246 27.29111365535272 28.66041429206477 22.015226351090043 16-17 19.51895963706293 30.700608790811827 29.142359303449815 20.63807226867543 18-19 19.62629333682388 28.72752570949188 32.62574680559723 19.020434148087013 20-21 20.865531522457452 27.56997966430161 32.600015724573474 18.964473088667468 22-23 20.413010818265466 27.727526758791566 32.439454050527736 19.42000837241523 24-25 20.134353130691466 28.015666739630774 32.744123378762346 19.105856750915414 26-27 20.04050381131089 27.96837047599416 32.81845749756148 19.172668215133466 28-29 19.592437787264423 28.339938334434756 32.78287643725178 19.28474744104904 30-31 20.18702424226197 27.890734934807565 32.58555086217045 19.336689960760015 32-33 20.029494889394165 27.858815529441767 32.61981767568371 19.491871905480355 34-35 20.283031296721752 28.087619941301117 32.332196924300874 19.297151837676253 36-37 20.660569019304496 27.60708426483964 32.25384857320152 19.478498142654345 38-39 19.853429035451352 28.499092751061855 32.082184130093 19.565294083393795 40-41 20.258698543014496 28.18374260361945 32.050026157996 19.507532695370056 42-43 20.227995780752103 28.2531939723573 31.673513169850224 19.84529707704037 44-45 20.593440106484163 28.223997524889228 31.4364567157354 19.746105652891213 46-47 20.897272441373214 28.505155077304913 30.812360285930424 19.785212195391455 48-49 20.577701561234964 28.6752984026401 30.69625511858543 20.05074491753951 50-51 20.438871073653072 28.902758563532387 30.30705249579766 20.35131786701688 52-53 20.902488965272802 29.250718011615685 29.860613524882428 19.986179498229088 54-55 20.974288947906825 28.89691566824213 29.74644245517449 20.382352928676557 56-57 21.050940539125413 29.38283242504438 29.459161846849007 20.107065188981192 58-59 21.03842136085888 28.96591350497702 29.735054700057034 20.260610434107065 60-61 20.889949867486283 29.095302775563077 29.959956888418183 20.054790468532456 62-63 20.577517597447915 28.894707717886625 30.26565537477613 20.262119309889336 64-65 20.540139446210024 28.96492255491024 30.027556053449565 20.467381945430173 66-67 20.749886418838027 29.189657378015532 29.309167285395354 20.75128891775109 68-69 20.81591594093018 28.940353895347748 29.63049062223227 20.6132395414898 70-71 20.81159876073625 28.910119379008165 29.7980034084003 20.480278451855284 72-73 20.980218663117196 28.637082633171072 29.81667010904257 20.566028594669163 74-75 20.616619295000817 29.45214328455638 29.221078918022087 20.710158502420715 76-77 20.498498529128025 29.35080190359138 29.328915192607308 20.821784374673292 78-79 20.741903663788435 29.334532397032316 28.849523597429094 21.074040341750155 80-81 20.71249525062666 28.9978248507613 28.923152376896667 21.366527521715366 82-83 20.757080387776405 29.206068272313974 29.158548320735022 20.8783030191746 84-85 20.617413505622917 29.157385105213695 28.902792990258767 21.322408398904617 86-87 20.211818717722807 29.06032273049704 29.127135007125364 21.600723544654784 88-89 20.55497379528876 28.82220745155571 29.11710607510714 21.50571267804839 90-91 20.31025087558473 28.766055574184264 29.549268826718585 21.37442472351242 92-93 20.24203799459333 28.80852215812962 29.59483115095968 21.35460869631737 94-95 20.1024553064862 28.73210920746307 29.494404226727628 21.6710312593231 96-97 20.340951011242066 28.81351394080957 29.208635294957645 21.636899752990715 98-99 20.024581824239956 28.928140663794256 28.89826337741685 22.14901413454894 100-101 19.8144848993802 28.92963753752631 28.890326287624053 22.36555127546944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 542.0 1 379.0 2 244.0 3 264.0 4 242.0 5 261.5 6 392.0 7 706.0 8 1163.5 9 1450.0 10 1382.0 11 1285.5 12 1358.0 13 1465.5 14 1616.5 15 1815.5 16 2033.5 17 2238.5 18 2432.0 19 2745.5 20 3140.5 21 3596.0 22 4226.5 23 5218.5 24 6314.0 25 8176.0 26 11965.0 27 15981.0 28 17538.0 29 19204.5 30 22427.5 31 25846.5 32 29057.0 33 32473.0 34 37463.5 35 42147.5 36 46997.0 37 50217.0 38 48120.5 39 45045.0 40 44026.0 41 43264.0 42 41835.5 43 41015.0 44 41386.0 45 41863.5 46 40889.0 47 39671.0 48 40210.0 49 38264.5 50 33788.0 51 30446.0 52 27958.0 53 25646.0 54 23542.5 55 21271.5 56 18462.0 57 15920.5 58 14468.0 59 13166.0 60 10892.0 61 8569.0 62 6443.5 63 4412.0 64 2955.5 65 2084.0 66 1489.5 67 1083.0 68 827.0 69 598.0 70 411.5 71 264.0 72 159.5 73 124.0 74 76.5 75 49.0 76 36.5 77 21.0 78 9.5 79 6.0 80 5.0 81 1.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026944028667766524 2 0.005864788574371894 3 8.499693586046223E-5 4 2.549908075813867E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 8.499693586046223E-5 10-11 0.012282057231836794 12-13 0.0 14-15 0.0 16-17 4.2498467930231115E-5 18-19 1.6999387172092446E-4 20-21 8.924678265348533E-4 22-23 0.0025924065437440977 24-25 0.011517084809092634 26-27 0.006842253336767209 28-29 4.674831472325423E-4 30-31 0.002932394287185947 32-33 0.003229883562697565 34-35 0.0 36-37 0.008754684393627608 38-39 0.013132026590441414 40-41 7.224739548139289E-4 42-43 0.0 44-45 0.0 46-47 2.549908075813867E-4 48-49 0.00344237590234872 50-51 0.007267238016069521 52-53 0.0 54-55 0.002932394287185947 56-57 0.0028473973513254845 58-59 5.099816151627734E-4 60-61 0.002634905011674329 62-63 0.005439803895069583 64-65 1.6999387172092446E-4 66-67 0.003527372838209182 68-69 5.949785510232356E-4 70-71 6.799754868836978E-4 72-73 0.0 74-75 8.499693586046223E-5 76-77 0.0 78-79 0.003357378966488258 80-81 0.0035698713061394134 82-83 0.014024494416976268 84-85 0.010327127707046161 86-87 0.007012247208488134 88-89 5.949785510232356E-4 90-91 0.00114745863411624 92-93 2.974892755116178E-4 94-95 0.0 96-97 0.003314880498558027 98-99 0.0028898958192557157 100-101 4.249846793023111E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1176513.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.85200387459079 #Duplication Level Percentage of deduplicated Percentage of total 1 75.95690924775307 40.90431771111939 2 14.04082873931313 15.12253527343913 3 4.588044646925192 7.4122619430903285 4 1.9654290008485187 4.233691606757102 5 0.9943019714614779 2.6772576809828386 6 0.5588924566923203 1.8058487245964645 7 0.36832053989394165 1.3884359399021944 8 0.23756324432563325 1.023460540310749 9 0.17774368007844213 0.8614668013441464 >10 0.928752345286828 9.546910324733497 >50 0.10454346213090958 3.9154385840101473 >100 0.0720623336304552 7.074143201632651 >500 0.004877578130101458 1.7210643040004656 >1k 0.0014160710700294554 1.1698017812638062 >5k 3.1468246000654565E-4 1.1433655828171665 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7529 0.6399419300934202 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5965 0.5070067224076572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3831 0.3256232612814308 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1642 0.139564968682879 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1332 0.11321591856613569 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1254 0.10658615756901965 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1246 0.10590618208213595 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1241 0.10548119740283361 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.499693586046223E-5 7 0.0 0.0 0.0 0.0 8.499693586046223E-5 8 0.0 0.0 0.0 0.0 8.499693586046223E-5 9 0.0 0.0 0.0 0.0 8.499693586046223E-5 10-11 0.0 0.0 0.0 0.0 8.499693586046223E-5 12-13 0.0 0.0 0.0 0.0 3.399877434418489E-4 14-15 0.0 0.0 0.0 0.0 4.249846793023112E-4 16-17 0.0 0.0 0.0 1.2749540379069336E-4 4.249846793023112E-4 18-19 4.2498467930231115E-5 0.0 0.0 2.549908075813867E-4 4.249846793023112E-4 20-21 8.499693586046223E-5 0.0 0.0 3.399877434418489E-4 4.249846793023112E-4 22-23 8.499693586046223E-5 0.0 0.0 7.6497242274416E-4 4.249846793023112E-4 24-25 8.499693586046223E-5 0.0 0.0 0.0011899571020464713 4.249846793023112E-4 26-27 8.499693586046223E-5 0.0 0.0 0.0017424371851394757 5.099816151627734E-4 28-29 8.499693586046223E-5 0.0 0.0 0.003229883562697565 5.099816151627734E-4 30-31 8.499693586046223E-5 0.0 0.0 0.006799754868836978 5.099816151627734E-4 32-33 8.499693586046223E-5 0.0 0.0 0.01070961391841824 5.099816151627734E-4 34-35 8.499693586046223E-5 0.0 0.0 0.014194488288697193 5.949785510232356E-4 36-37 8.499693586046223E-5 0.0 0.0 0.020016778395138858 5.949785510232356E-4 38-39 8.499693586046223E-5 0.0 0.0 0.02851647198118508 5.949785510232356E-4 40-41 8.499693586046223E-5 0.0 0.0 0.03918358743167309 5.949785510232356E-4 42-43 8.499693586046223E-5 0.0 0.0 0.05754292557753293 5.949785510232356E-4 44-45 8.499693586046223E-5 0.0 0.0 0.07250238628897429 5.949785510232356E-4 46-47 1.2749540379069336E-4 0.0 0.0 0.1027187969873686 5.949785510232356E-4 48-49 1.6999387172092446E-4 0.0 0.0 0.1387574977922046 5.949785510232356E-4 50-51 1.6999387172092446E-4 0.0 0.0 0.15490691560569242 5.949785510232356E-4 52-53 1.6999387172092446E-4 0.0 0.0 0.19043563479536563 6.799754868836978E-4 54-55 1.6999387172092446E-4 0.0 0.0 0.2485735389239218 7.6497242274416E-4 56-57 1.6999387172092446E-4 0.0 0.0 0.3395627587625466 7.6497242274416E-4 58-59 1.6999387172092446E-4 0.0 0.0 0.45626355169896127 7.6497242274416E-4 60-61 1.6999387172092446E-4 0.0 0.0 0.5904737134226311 7.6497242274416E-4 62-63 1.6999387172092446E-4 0.0 0.0 0.7280412541127892 7.6497242274416E-4 64-65 1.6999387172092446E-4 0.0 0.0 0.8806532524502492 8.074708906743912E-4 66-67 1.6999387172092446E-4 0.0 0.0 1.065606584882615 8.499693586046223E-4 68-69 1.6999387172092446E-4 0.0 0.0 1.3033005160163975 8.499693586046223E-4 70-71 1.6999387172092446E-4 8.499693586046223E-5 0.0 1.4389556256496954 8.499693586046223E-4 72-73 1.6999387172092446E-4 8.499693586046223E-5 0.0 1.6094169805178522 8.499693586046223E-4 74-75 1.6999387172092446E-4 8.499693586046223E-5 0.0 1.7995551260377063 8.924678265348535E-4 76-77 1.6999387172092446E-4 8.499693586046223E-5 0.0 2.0020178272573275 9.774647623953157E-4 78-79 1.6999387172092446E-4 8.499693586046223E-5 0.0 2.2230948574303895 0.0010199632303255469 80-81 1.6999387172092446E-4 8.499693586046223E-5 0.0 2.5393259572992393 0.001062461698255778 82-83 1.6999387172092446E-4 8.499693586046223E-5 0.0 2.8694115577133443 0.001104960166186009 84-85 1.6999387172092446E-4 8.499693586046223E-5 0.0 3.3509191993628633 0.001104960166186009 86-87 1.6999387172092446E-4 8.499693586046223E-5 0.0 3.9656595379736563 0.00114745863411624 88-89 1.6999387172092446E-4 8.499693586046223E-5 0.0 4.668626696007609 0.0011899571020464713 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5270 0.0 38.66702 1 TATCCCG 75 6.912289E-6 38.00811 5 GTATCAA 8420 0.0 33.001812 1 ATCAACG 10590 0.0 26.191206 3 TCAACGC 10600 0.0 26.166498 4 TATCAAC 10770 0.0 25.841667 2 CAACGCA 10765 0.0 25.73006 5 AACGCAG 11050 0.0 25.10943 6 CATTCCG 285 0.0 25.005337 9 GTACATG 6410 0.0 23.19425 1 TACATGG 6435 0.0 22.510748 2 ACGCAGA 12325 0.0 22.357712 7 GAGTACT 6850 0.0 22.22164 12-13 CGCAGAG 12345 0.0 22.167551 8 AGTACTT 7230 0.0 21.874825 12-13 ACATGGG 6245 0.0 21.8267 3 AGAGTAC 10475 0.0 21.612011 10-11 GCAGAGT 12885 0.0 20.832928 9 TACTCCG 115 0.0037187284 20.656582 7 GTACTTT 7520 0.0 19.989157 14-15 >>END_MODULE