FastQCFastQC Report
Thu 26 May 2016
SRR937100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221747
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69590.5695941958523328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.4236556341042786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32630.26707657149966396No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC14380.1177003094748749No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13810.11303485909930616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3550.036.149679
GTATCAA82200.035.4495661
GGTATCA50750.033.7200931
ATCAACG98950.029.1136673
TCAACGC98850.029.095034
TATCAAC100200.028.7979162
CAACGCA99650.028.766045
AACGCAG102550.027.9514246
ACGCAGA114300.024.8670257
CGCAGAG113150.024.7837128
GAGTACT55600.023.637812-13
GCAGAGT117950.023.4124629
CATGGGG23250.023.3089144
TACATGG68350.022.534432
ACATGGG67500.022.2547863
GTACATG70150.022.2263661
AGTACTT59200.022.07993112-13
GTACTTT59750.022.03398114-15
AGAGTAC94150.021.75834510-11
GTGTAGC9400.021.7451741
Produced by FastQC (version 0.11.5)