##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937100_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1221747 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.832814813541592 33.0 31.0 34.0 30.0 34.0 2 31.914485568616087 33.0 31.0 34.0 30.0 34.0 3 31.93281342209148 33.0 31.0 34.0 30.0 34.0 4 35.55438728312818 37.0 35.0 37.0 33.0 37.0 5 35.56638076459365 37.0 35.0 37.0 33.0 37.0 6 35.631667603849245 37.0 35.0 37.0 33.0 37.0 7 35.59406407382216 37.0 35.0 37.0 33.0 37.0 8 35.550375814305255 37.0 35.0 37.0 33.0 37.0 9 37.17392635300107 39.0 37.0 39.0 34.0 39.0 10-11 37.05880268173361 39.0 37.0 39.0 33.0 39.0 12-13 37.00103294708315 39.0 37.0 39.0 33.0 39.0 14-15 38.15079635963911 40.0 38.0 41.0 33.0 41.0 16-17 38.09877658795152 40.0 38.0 41.0 33.0 41.0 18-19 38.107326639639794 40.0 38.0 41.0 33.0 41.0 20-21 38.0687863362873 40.0 38.0 41.0 33.0 41.0 22-23 37.962286790964086 40.0 38.0 41.0 33.0 41.0 24-25 37.93208250153264 40.0 38.0 41.0 33.0 41.0 26-27 37.93948460687851 40.0 38.0 41.0 33.0 41.0 28-29 37.86238026367161 40.0 38.0 41.0 33.0 41.0 30-31 37.69275594701685 40.0 37.0 41.0 33.0 41.0 32-33 37.46308810252859 40.0 37.0 41.0 32.0 41.0 34-35 37.29910038657758 40.0 36.0 41.0 32.0 41.0 36-37 37.11664157963965 39.5 36.0 41.0 31.0 41.0 38-39 36.95296366596358 39.0 36.0 41.0 31.0 41.0 40-41 36.77704017280173 39.0 36.0 41.0 30.5 41.0 42-43 36.528461293541135 39.0 35.5 41.0 30.0 41.0 44-45 36.2357628052289 39.0 35.0 41.0 30.0 41.0 46-47 35.84363947691298 39.0 35.0 41.0 28.5 41.0 48-49 35.56912969706494 39.0 35.0 40.5 27.0 41.0 50-51 34.858956068645966 38.5 34.0 40.0 26.0 40.5 52-53 35.05550740046834 38.5 35.0 40.0 26.5 40.5 54-55 35.46059699757806 39.0 35.0 40.5 27.0 41.0 56-57 35.38208156025757 39.0 35.0 41.0 26.5 41.0 58-59 35.20497533450052 39.0 35.0 41.0 26.0 41.0 60-61 34.96651066055411 39.0 35.0 41.0 26.0 41.0 62-63 34.68910993847335 38.0 34.5 40.0 25.5 41.0 64-65 34.400478986238554 38.0 34.0 40.0 24.5 41.0 66-67 34.04849531040387 37.5 34.0 40.0 23.0 41.0 68-69 33.66012234938985 37.0 34.0 39.5 22.0 41.0 70-71 33.24967485084883 36.5 33.0 39.0 22.0 41.0 72-73 32.86954132074808 36.0 33.0 39.0 20.5 40.5 74-75 32.48096905496801 35.5 32.5 38.5 20.0 40.0 76-77 32.02412569869212 35.0 32.0 37.0 18.5 39.5 78-79 31.567757481704476 35.0 32.0 37.0 16.5 39.0 80-81 31.12712943023392 35.0 31.0 36.5 12.0 39.0 82-83 30.709344897102262 35.0 31.0 36.0 7.5 37.5 84-85 30.332173109489936 34.5 31.0 35.5 7.0 37.0 86-87 30.002309397935907 34.0 31.0 35.0 2.0 37.0 88-89 29.642287232954125 34.0 30.0 35.0 2.0 36.0 90-91 29.282075175957054 34.0 30.0 35.0 2.0 36.0 92-93 28.93248765906526 34.0 29.0 35.0 2.0 35.5 94-95 28.590402923027433 34.0 29.0 35.0 2.0 35.0 96-97 28.150229139093447 34.0 29.0 35.0 2.0 35.0 98-99 27.657753610199165 34.0 29.0 35.0 2.0 35.0 100-101 25.62040217819238 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35340816143186515 1101 2 0.3772512946399118 1101 3 0.31363151438546666 1101 4 0.29372362953419895 1101 5 0.30298296739063346 1101 6 0.2962480101328282 1101 7 0.2670501926653017 1101 8 0.31320150042025574 1101 9 0.33445528111531786 1101 10-11 0.31350219699763215 1101 12-13 0.4226374456618558 1101 14-15 0.5661864088008244 1101 16-17 0.6047218481042549 1101 18-19 0.46663535561438607 1101 20-21 0.4762687611844214 1101 22-23 0.5023580880865879 1101 24-25 0.4692298178743215 1101 26-27 0.43367890851600777 1101 28-29 0.43229104899034354 1101 30-31 0.41589519183239787 1101 32-33 0.2661458011635176 1101 34-35 0.42718783906993707 1101 36-37 0.3698826805144151 1101 38-39 0.3297420482356799 1101 40-41 0.46277314063260633 1101 42-43 0.3695434801910338 1101 44-45 0.3584651006486226 1101 46-47 0.440919166103825 1101 48-49 0.6544027766105174 1101 50-51 0.7467187219182279 1101 52-53 0.7346490078536476 1101 54-55 0.8683082566436795 1101 56-57 0.8395085681942973 1101 58-59 0.8861173317472719 1101 60-61 0.866085246088808 1101 62-63 0.8474930798284745 1101 64-65 0.7257261129950692 1101 66-67 0.7891596546289321 1101 68-69 0.8842006418980972 1101 70-71 0.9588802352920069 1101 72-73 0.8757749624918674 1101 74-75 0.7364366387493959 1101 76-77 0.7922828371732855 1101 78-79 0.907637475034889 1101 80-81 1.0605125645022575 1101 82-83 0.9979880892969248 1101 84-85 0.9766570188298118 1101 86-87 1.0399607636200194 1101 88-89 1.071920592911603 1101 90-91 1.1385725184382984 1101 92-93 1.1878895619625531 1101 94-95 1.1491222491552655 1101 96-97 1.2757415829098342 1101 98-99 1.2243126624691278 1101 100-101 1.356084551183514 1102 1 0.18623194580497326 1102 2 0.140805169912241 1102 3 0.12484028904728461 1102 4 0.1565180417770975 1102 5 0.31108317572118693 1102 6 0.14574252739872406 1102 7 0.13434667963653624 1102 8 0.014216182673280287 1102 9 0.2388624348777526 1102 10-11 0.2288948324267821 1102 12-13 -0.0011577442467824994 1102 14-15 -0.10350959632263113 1102 16-17 0.09280836151231853 1102 18-19 0.018557648127256243 1102 20-21 0.048753212634629506 1102 22-23 -0.035869996701848095 1102 24-25 -0.01669619523543986 1102 26-27 -0.059304230623261844 1102 28-29 0.02614463054137417 1102 30-31 0.024626395861012895 1102 32-33 -0.030707368682108438 1102 34-35 -0.022494042271226533 1102 36-37 -0.02862836596064966 1102 38-39 0.06390997447941515 1102 40-41 0.1455086275409485 1102 42-43 0.021980976259818874 1102 44-45 0.11642378524988573 1102 46-47 0.06699250684532387 1102 48-49 0.11385837246830732 1102 50-51 -0.00784529922361088 1102 52-53 0.24132563995104306 1102 54-55 -0.045524520442626226 1102 56-57 0.08308710766940663 1102 58-59 -0.2283305732250227 1102 60-61 -0.12976577373976284 1102 62-63 -0.22743920797646666 1102 64-65 -0.046180283115077714 1102 66-67 -0.31199169094247026 1102 68-69 -0.1025864263592311 1102 70-71 -0.1284220365212363 1102 72-73 -0.10940054599289084 1102 74-75 0.067764655492077 1102 76-77 0.02208163522226414 1102 78-79 -0.16251874428971647 1102 80-81 -0.25635644456479056 1102 82-83 -0.09517884008607069 1102 84-85 -0.3500626612598481 1102 86-87 -0.15440563893255188 1102 88-89 -0.12207865801330442 1102 90-91 -0.30418208608626784 1102 92-93 -0.090941966665266 1102 94-95 -0.19478827544508093 1102 96-97 -0.3830363668354231 1102 98-99 -0.0655751096900552 1102 100-101 -0.16522167948205535 1103 1 0.28312840636365166 1103 2 0.3202476133217118 1103 3 0.28924531696209854 1103 4 0.310322887474733 1103 5 0.2900304941649523 1103 6 0.28497149783166265 1103 7 0.24645422675637008 1103 8 0.2762673304266272 1103 9 0.2101844690132353 1103 10-11 0.29960669388575667 1103 12-13 0.24325517008364983 1103 14-15 0.4843367275231927 1103 16-17 0.3818561143086683 1103 18-19 0.2908853751753071 1103 20-21 0.16422560543210096 1103 22-23 0.19480066348310032 1103 24-25 0.243181690265601 1103 26-27 0.28746278876642606 1103 28-29 0.30947523178310377 1103 30-31 0.333966300874188 1103 32-33 0.33101285447646234 1103 34-35 0.4797296970392537 1103 36-37 0.29570897271892704 1103 38-39 0.33613774695682963 1103 40-41 0.3482058766412379 1103 42-43 0.4864921059513776 1103 44-45 0.5004436232335223 1103 46-47 0.4702054965414959 1103 48-49 0.6606241174372158 1103 50-51 0.5853403478997734 1103 52-53 0.6412766122341367 1103 54-55 0.7532852954960276 1103 56-57 0.7857053926588762 1103 58-59 0.8197983022010931 1103 60-61 0.9006566492689814 1103 62-63 0.9812796792892655 1103 64-65 1.05882274300955 1103 66-67 0.8214716499690766 1103 68-69 0.8684752261197204 1103 70-71 0.8603203300731437 1103 72-73 0.7631362610283148 1103 74-75 0.8250237312421476 1103 76-77 0.7722780459535876 1103 78-79 0.944239109265844 1103 80-81 0.8280523166532348 1103 82-83 0.8717252020267914 1103 84-85 0.6081021028548577 1103 86-87 0.7569351566758407 1103 88-89 0.8499254411236379 1103 90-91 0.7517103857852696 1103 92-93 0.7155193716564661 1103 94-95 0.952888355526138 1103 96-97 0.9959296431130547 1103 98-99 1.0287961235844172 1103 100-101 1.008975897719104 1104 1 0.2012937211696304 1104 2 0.07592330401249825 1104 3 0.09336849205910269 1104 4 0.22801270897683423 1104 5 0.19590242796844137 1104 6 0.01243649215624032 1104 7 0.184686938517018 1104 8 0.08405093249810136 1104 9 0.10103179481274793 1104 10-11 0.1280799736675391 1104 12-13 0.11647983168274578 1104 14-15 -0.021330520846625234 1104 16-17 0.2178903095212732 1104 18-19 0.10841699847567554 1104 20-21 0.2644347298926135 1104 22-23 0.16689920116417767 1104 24-25 -0.025201055155392282 1104 26-27 0.017562195734221575 1104 28-29 0.047465621698506766 1104 30-31 0.2896535112481118 1104 32-33 0.008875400419029233 1104 34-35 0.09656324932826976 1104 36-37 0.11045245143447602 1104 38-39 0.10668199888004182 1104 40-41 0.027073603445550987 1104 42-43 0.0904089071505112 1104 44-45 0.012545145683567682 1104 46-47 -0.08725675266710198 1104 48-49 0.0037154121515641236 1104 50-51 -0.04761722045034844 1104 52-53 0.12036661717969821 1104 54-55 0.18969528292513615 1104 56-57 0.1445553688025143 1104 58-59 0.15389294225320782 1104 60-61 0.14682138455047067 1104 62-63 -0.08934687164679644 1104 64-65 0.11845031138145146 1104 66-67 -0.09873180451972274 1104 68-69 -0.13870319770440176 1104 70-71 -0.06707924988053549 1104 72-73 -0.005779303779299028 1104 74-75 0.11285540552324846 1104 76-77 0.2177643454283107 1104 78-79 0.31990918445151806 1104 80-81 0.11559831368589357 1104 82-83 0.17485408957942994 1104 84-85 0.21505242061292762 1104 86-87 0.41679186597895423 1104 88-89 0.33975748250952975 1104 90-91 0.26800175043470986 1104 92-93 0.2573488934180439 1104 94-95 0.16605137728881303 1104 96-97 0.129237295450654 1104 98-99 0.17599929157897876 1104 100-101 0.03803463884377578 1105 1 0.032143215187062424 1105 2 0.15097706704336034 1105 3 0.13641545781599262 1105 4 0.13674424461676438 1105 5 0.21761735426352402 1105 6 0.08220578139118118 1105 7 0.1384710678736667 1105 8 0.06015561661804725 1105 9 0.04235192506418173 1105 10-11 0.16922648618810143 1105 12-13 0.09420058421265765 1105 14-15 0.15986550958604795 1105 16-17 0.18332579642622449 1105 18-19 0.03840987455634348 1105 20-21 -0.021363282527538274 1105 22-23 0.1833520549261891 1105 24-25 0.14784695281628046 1105 26-27 0.16554096632749804 1105 28-29 0.20575421605580146 1105 30-31 0.22125673626737097 1105 32-33 0.2278895287843028 1105 34-35 -0.02931913800450303 1105 36-37 -0.10394145618462147 1105 38-39 0.2694200242134812 1105 40-41 0.3240863870995483 1105 42-43 0.348320207046541 1105 44-45 0.11503727823294696 1105 46-47 0.04407629087968701 1105 48-49 0.07854420930860329 1105 50-51 0.071224815376425 1105 52-53 0.08284720275189272 1105 54-55 0.1860084481314388 1105 56-57 0.20789708433888876 1105 58-59 0.31697238825249485 1105 60-61 0.4811985214085226 1105 62-63 0.4398754963160343 1105 64-65 0.4898858097253509 1105 66-67 0.493027898881202 1105 68-69 0.27991603293772727 1105 70-71 0.2966832598743423 1105 72-73 0.39495402777465216 1105 74-75 0.4761082468295257 1105 76-77 0.42432654912792245 1105 78-79 0.38420192097707684 1105 80-81 0.19511231113882488 1105 82-83 0.10839700976357136 1105 84-85 0.27761231618809035 1105 86-87 0.3653617439381449 1105 88-89 0.3335494729618276 1105 90-91 0.4796121819878927 1105 92-93 0.38152338305055267 1105 94-95 0.27340092977194885 1105 96-97 0.19687052100372426 1105 98-99 0.411434181116924 1105 100-101 0.38900968161271265 1106 1 0.35053188271632507 1106 2 0.33957932341250086 1106 3 0.2899699394811215 1106 4 0.30937691369990006 1106 5 0.32138198123792705 1106 6 0.3821377245489401 1106 7 0.3977175257270318 1106 8 0.45064513333737466 1106 9 0.4590816588801232 1106 10-11 0.3826315854401585 1106 12-13 0.40561374663722916 1106 14-15 0.39741065774138207 1106 16-17 0.40532044867460826 1106 18-19 0.41967252813930145 1106 20-21 0.2907302341695228 1106 22-23 0.2502497036886382 1106 24-25 0.2639624618527563 1106 26-27 0.07673492490148703 1106 28-29 0.2501982464770762 1106 30-31 0.3866991078834232 1106 32-33 0.39065545479208197 1106 34-35 0.2681059662757974 1106 36-37 0.39316923319392316 1106 38-39 0.3925525834377126 1106 40-41 0.5243431100148328 1106 42-43 0.39919858574325673 1106 44-45 0.32627303886987846 1106 46-47 0.46806356523526915 1106 48-49 0.5575283934809576 1106 50-51 0.46017759232258015 1106 52-53 0.34092204643316393 1106 54-55 0.4987300665222918 1106 56-57 0.5015685689502831 1106 58-59 0.3485104600381135 1106 60-61 0.47398209765210453 1106 62-63 0.46462951301887045 1106 64-65 0.10545737798370425 1106 66-67 0.1162773839577298 1106 68-69 0.35358724343723935 1106 70-71 0.41000047200224543 1106 72-73 0.3004462449243732 1106 74-75 0.5364477686670384 1106 76-77 0.31002210590269996 1106 78-79 0.36236741003337514 1106 80-81 0.3833927001892512 1106 82-83 0.39076673273496354 1106 84-85 0.614631597817926 1106 86-87 0.7604811817689274 1106 88-89 0.73523795936309 1106 90-91 0.46466773033373343 1106 92-93 0.708030687918221 1106 94-95 0.7238766209729164 1106 96-97 0.7799431268184094 1106 98-99 0.7788492588983154 1106 100-101 0.9581126461756142 1107 1 0.3005711458725884 1107 2 0.24464256347643953 1107 3 0.23952811258042672 1107 4 0.22898397175660534 1107 5 0.29387976977557173 1107 6 0.2167793124565094 1107 7 0.2941167994362388 1107 8 0.35336934458246816 1107 9 0.21513237508354166 1107 10-11 0.2973818065863938 1107 12-13 0.2425478062613564 1107 14-15 0.3895555434436986 1107 16-17 0.40773303771506875 1107 18-19 0.41941336026991394 1107 20-21 0.3336142914657856 1107 22-23 0.40600369596015895 1107 24-25 0.48390597256864964 1107 26-27 0.5366570193503222 1107 28-29 0.38557605736213674 1107 30-31 0.47021789367419586 1107 32-33 0.5050294945518488 1107 34-35 0.48748799833812484 1107 36-37 0.5680464000390586 1107 38-39 0.5398126979681805 1107 40-41 0.6013215540764776 1107 42-43 0.6409329929937471 1107 44-45 0.643099396397389 1107 46-47 0.6475300208430781 1107 48-49 0.7322301952408097 1107 50-51 0.6442631332385105 1107 52-53 0.7246500288035378 1107 54-55 0.764412516046626 1107 56-57 0.8304637689130416 1107 58-59 0.961561036570231 1107 60-61 0.978871214153493 1107 62-63 0.8941619245215762 1107 64-65 1.0096214452121615 1107 66-67 0.9768751771510864 1107 68-69 0.8358086379776495 1107 70-71 0.7574086381179868 1107 72-73 0.7546027078261233 1107 74-75 0.8835346907770045 1107 76-77 0.9850504281399246 1107 78-79 0.821177720879632 1107 80-81 0.9394750752222301 1107 82-83 0.959020065447536 1107 84-85 0.9866361914520176 1107 86-87 1.0193330836265524 1107 88-89 0.8869908342641608 1107 90-91 0.943759556458474 1107 92-93 0.9991463512793324 1107 94-95 0.9854694946324578 1107 96-97 1.0035540956717064 1107 98-99 1.3179590871026043 1107 100-101 1.3951742722600038 1108 1 -0.011676628853596327 1108 2 0.049017666881358224 1108 3 0.02625125211915602 1108 4 -0.012330346101840917 1108 5 -0.014141804626333965 1108 6 0.021951212790710883 1108 7 -0.08506456451598154 1108 8 0.02767793794470208 1108 9 0.0368086928758089 1108 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68-69 -1.8138155326268617 2316 70-71 -1.5446054156978377 2316 72-73 -1.6370364959542059 2316 74-75 -1.9537167354159806 2316 76-77 -2.1477556830664035 2316 78-79 -2.41745656161676 2316 80-81 -2.1841304385745026 2316 82-83 -2.5625150813340447 2316 84-85 -2.7819542646848987 2316 86-87 -2.9822573969943598 2316 88-89 -2.6922271467852283 2316 90-91 -3.1214134229994492 2316 92-93 -3.1745720337560464 2316 94-95 -3.0593274105899795 2316 96-97 -3.574475921711933 2316 98-99 -3.944366892869482 2316 100-101 -4.25235798540961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1836.0 3 620.0 4 574.0 5 625.0 6 709.0 7 865.0 8 1144.0 9 1500.0 10 1940.0 11 2717.0 12 4039.0 13 5271.0 14 6114.0 15 7840.0 16 10636.0 17 14718.0 18 12379.0 19 6545.0 20 6038.0 21 6124.0 22 6640.0 23 7431.0 24 8382.0 25 9640.0 26 11385.0 27 13596.0 28 16220.0 29 20023.0 30 24566.0 31 30930.0 32 39447.0 33 51778.0 34 70506.0 35 100219.0 36 152686.0 37 244775.0 38 266789.0 39 54484.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28065592992322 20.845025215162014 15.267229951305547 21.60708890360922 2 14.269900726640058 21.514195067035104 42.069470883225875 22.146433323098965 3 17.768906623919204 27.250361664170974 32.24513421486331 22.735597497046513 4 11.321712711028125 18.537144635391716 41.11038772259552 29.030754930984642 5 11.751466403605725 39.03964185258045 35.913799474478964 13.295092269334857 6 27.148752033503115 39.596618545548424 19.61530423665264 13.639325184295824 7 25.01130269266177 35.44229565878317 22.848393215763487 16.69800843279157 8 24.786835979841694 34.86754622343079 21.832895138950906 18.512722657776607 9 26.039985648026033 16.90862412737213 22.5724111152434 34.478979109358434 10-11 22.891727634434233 27.838656800414835 29.775658275765178 19.49395728938575 12-13 24.27217192392522 26.402175671552236 29.384017087540883 19.941635316981664 14-15 21.74871032899921 27.11159565353433 28.66800616617548 22.47168785129098 16-17 19.589151153162938 30.39623007731978 29.161619710523794 20.85299905899349 18-19 19.610074694866615 28.689438656139465 32.30255045353819 19.39793619545573 20-21 20.933089543590384 27.605216226896452 32.06071265502182 19.40098157449135 22-23 20.401082293351607 27.82184983077396 31.83029823658566 19.94676963928877 24-25 19.92302884719675 28.154145313645373 32.20223202427256 19.72059381488532 26-27 19.8920365716905 28.08060014242332 32.21938921675357 19.80797406913261 28-29 19.56691856353302 28.316705739057547 32.284408806349376 19.831966891060063 30-31 20.097677689001376 27.954498387863836 32.06059569229359 19.8872282308412 32-33 19.845432131911885 27.894535649736753 32.13420384000838 20.12582837834298 34-35 20.26662213687345 28.1412331151046 31.698582413678505 19.89356233434344 36-37 20.493567545377935 27.69559200970158 31.6952023748083 20.11563807011218 38-39 19.689889358455847 28.525374214307526 31.597790934383678 20.18694549285295 40-41 20.17367331294307 28.154116340737595 31.66400840167264 20.008201944646697 42-43 20.010992802292854 28.249987619796375 31.300838129553814 20.43818144835695 44-45 20.49088848663384 28.226272147503096 30.937237435475375 20.345601930387687 46-47 20.561691253567712 28.468038119996304 30.559432145309774 20.410838481126213 48-49 20.303263700519423 28.448273039643396 30.6389565063655 20.60950675347168 50-51 20.15473260864494 28.787640053514696 30.203512178112753 20.854115159727613 52-53 20.53630554166295 29.11998546255831 29.74977755913407 20.593931436644667 54-55 20.51117596257352 28.747620938199645 29.79453092064064 20.946672178586194 56-57 20.64805298828572 29.166313654273786 29.581128796520385 20.604504560920116 58-59 20.684657797975895 28.786805994388036 29.72060513589528 20.80793107174079 60-61 20.57885235495643 28.804123399119515 30.040463759622266 20.576560486301787 62-63 20.271463840292853 28.73110320913922 30.325481567340844 20.671951383227082 64-65 20.245180834575784 28.752760985519892 30.049876791596702 20.952181388307622 66-67 20.389411032698572 28.980768915887545 29.478411081820116 21.151408969593767 68-69 20.52239936520051 28.587268143006767 29.79217522824366 21.098157263549062 70-71 20.47540541890762 28.703847026382018 29.87020698251709 20.950540572193272 72-73 20.49673756856672 28.449474364446925 30.009662674043746 21.044125392942608 74-75 20.275765472872664 29.233538654604708 29.426648979212604 21.064046893310024 76-77 20.234504230761203 29.000741319581604 29.579715596897344 21.18503885275985 78-79 20.323956513783177 29.008263887666473 29.193215809582213 21.474563788968137 80-81 20.30338055189441 28.805793359475153 29.245800010976613 21.645026077653824 82-83 20.450837808838514 29.07651691276316 29.41064975779258 21.061995520605745 84-85 20.44532217299211 28.786026379608504 29.233354139592947 21.535297307806438 86-87 20.12470037725344 28.927949301067894 29.342498224268265 21.604852097410397 88-89 20.543655482863976 28.55546574883654 29.366387665306465 21.53449110299302 90-91 20.154940367079533 28.451263716759083 29.855479886454038 21.538316029707342 92-93 20.364935410567693 28.50758490797797 29.858333162732613 21.269146518721723 94-95 20.114221301007877 28.538420859610863 29.74276672102268 21.60459111835858 96-97 20.224698864471307 28.545237152799242 29.672210799577403 21.557853183152048 98-99 20.153071251461682 28.59979069698885 29.31183855826583 21.935299493283633 100-101 20.183122861644918 28.618497178487445 29.41516436534297 21.783215594524663 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 430.0 1 303.5 2 202.5 3 229.0 4 223.0 5 252.5 6 377.0 7 672.0 8 1138.0 9 1400.0 10 1336.5 11 1270.0 12 1366.5 13 1500.5 14 1620.5 15 1749.0 16 1882.0 17 2079.5 18 2305.5 19 2555.0 20 2865.0 21 3369.0 22 3916.0 23 4728.5 24 5836.5 25 7686.5 26 11585.0 27 15753.5 28 17304.5 29 19138.5 30 22825.0 31 26882.5 32 30496.5 33 33732.5 34 38970.0 35 44201.0 36 49332.5 37 52946.5 38 50459.5 39 46908.5 40 45830.5 41 45217.5 42 44289.5 43 43492.5 44 43508.5 45 43754.0 46 42364.0 47 40752.0 48 41369.0 49 39928.5 50 35600.0 51 32004.5 52 29291.5 53 26918.0 54 25234.0 55 22832.5 56 19811.5 57 17068.0 58 15263.5 59 13789.0 60 11357.5 61 8938.5 62 6716.0 63 4582.5 64 2941.5 65 2083.5 66 1491.5 67 1084.5 68 810.0 69 574.0 70 418.5 71 271.5 72 166.5 73 124.5 74 79.5 75 51.0 76 42.0 77 24.5 78 11.5 79 6.0 80 6.0 81 8.0 82 6.0 83 4.0 84 3.0 85 2.5 86 1.0 87 1.5 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037487303017727895 2 0.030448202451080295 3 0.025373502042566914 4 0.05254770423009019 5 0.10182140819662336 6 0.12784971029190167 7 0.06539815526455149 8 0.09887480795942204 9 0.08258665664822587 10-11 0.08348700672070404 12-13 0.07976283142090793 14-15 0.0730920558839105 16-17 0.058440904704492834 18-19 0.06928603057752546 20-21 0.06134657993839968 22-23 0.03695527797489988 24-25 0.009862925793965527 26-27 0.0030284502437902447 28-29 0.006793550546880819 30-31 0.00622060050075834 32-33 0.006793550546880819 34-35 0.007612050612770074 36-37 0.00740742559629776 38-39 0.004419900355801978 40-41 0.006998175563353132 42-43 0.0031512252536736326 44-45 0.00192347515483975 46-47 0.002128100171312064 48-49 4.092500329446276E-4 50-51 0.0021690251746065265 52-53 0.0060569004875804895 54-55 0.01244120100151668 56-57 0.009699225780787675 58-59 0.005811350467813713 60-61 0.002987525240495782 62-63 0.009085350731370734 64-65 0.002987525240495782 66-67 0.023490951891021628 68-69 0.046981903782043255 70-71 0.05942310478355994 72-73 0.0029056752339068565 74-75 0.013546176090467175 76-77 0.02263152682183791 78-79 0.028524727296240546 80-81 0.07939450639125777 82-83 0.030816527480730462 84-85 0.013055076050933622 86-87 0.03433607776405426 88-89 0.049601103992888876 90-91 0.04644987873921524 92-93 0.046818203768865405 94-95 0.013996351126706264 96-97 0.020094176617581217 98-99 0.04509935363049797 100-101 0.0848375318294213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1221747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.8615753476485 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42220461126922 41.926425365145576 2 13.854545820466443 15.201644188739369 3 4.586496202263324 7.54867220946519 4 1.8589581015469636 4.079414798245614 5 0.9592533889847972 2.6313076038638314 6 0.5503617721587176 1.8116228299050507 7 0.3323355885559019 1.2762717752585304 8 0.22784003572087233 0.999973062952925 9 0.15873583204303113 0.7837648029000386 >10 0.8708552149790759 9.191741540092062 >50 0.10437606645776953 3.9899424587428545 >100 0.06822759852615157 7.033624067373849 >500 0.004171114789166107 1.4932231084921261 >1k 0.0013407154679462483 1.0406222888966254 >5k 2.9793677065472184E-4 0.9917498999262192 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6959 0.5695941958523328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5176 0.4236556341042786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3263 0.26707657149966396 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1438 0.1177003094748749 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1381 0.11303485909930616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.185000658892553E-5 2 0.0 0.0 0.0 0.0 8.185000658892553E-5 3 0.0 0.0 0.0 0.0 8.185000658892553E-5 4 0.0 0.0 0.0 0.0 8.185000658892553E-5 5 0.0 0.0 0.0 0.0 8.185000658892553E-5 6 0.0 0.0 0.0 0.0 8.185000658892553E-5 7 0.0 0.0 0.0 0.0 8.185000658892553E-5 8 0.0 0.0 0.0 0.0 8.185000658892553E-5 9 0.0 0.0 0.0 0.0 8.185000658892553E-5 10-11 0.0 0.0 0.0 0.0 8.185000658892553E-5 12-13 0.0 0.0 0.0 0.0 2.4555001976677657E-4 14-15 8.185000658892553E-5 0.0 0.0 0.0 2.4555001976677657E-4 16-17 8.185000658892553E-5 0.0 0.0 0.0 2.4555001976677657E-4 18-19 8.185000658892553E-5 0.0 0.0 8.185000658892553E-5 3.274000263557021E-4 20-21 8.185000658892553E-5 0.0 0.0 1.2277500988338829E-4 3.274000263557021E-4 22-23 8.185000658892553E-5 0.0 0.0 3.274000263557021E-4 4.092500329446276E-4 24-25 8.185000658892553E-5 0.0 0.0 7.366500593003298E-4 4.911000395335531E-4 26-27 8.185000658892553E-5 0.0 0.0 0.0014323751153061967 4.911000395335531E-4 28-29 1.2277500988338829E-4 0.0 0.0 0.0030693752470847073 4.911000395335531E-4 30-31 1.6370001317785106E-4 0.0 0.0 0.006261525504052803 4.911000395335531E-4 32-33 1.6370001317785106E-4 0.0 0.0 0.010272175826910153 4.911000395335531E-4 34-35 1.6370001317785106E-4 0.0 0.0 0.014855776195889982 4.911000395335531E-4 36-37 1.6370001317785106E-4 0.0 0.0 0.02042157664393692 4.911000395335531E-4 38-39 1.6370001317785106E-4 0.0 0.0 0.02962970238519104 4.911000395335531E-4 40-41 1.6370001317785106E-4 0.0 0.0 0.040024653221984584 4.911000395335531E-4 42-43 1.6370001317785106E-4 0.0 0.0 0.056640204559536465 4.911000395335531E-4 44-45 1.6370001317785106E-4 0.0 0.0 0.07149598075542646 5.729500461224787E-4 46-47 1.6370001317785106E-4 0.0 0.0 0.09920220798577775 6.548000527114042E-4 48-49 1.6370001317785106E-4 0.0 0.0 0.1380400361122229 6.548000527114042E-4 50-51 1.6370001317785106E-4 0.0 0.0 0.1567427626177924 8.185000658892553E-4 52-53 1.6370001317785106E-4 0.0 0.0 0.1882959401578232 8.185000658892553E-4 54-55 1.6370001317785106E-4 0.0 0.0 0.24194861947686389 8.185000658892553E-4 56-57 2.4555001976677657E-4 0.0 0.0 0.325885801233807 9.003500724781809E-4 58-59 2.4555001976677657E-4 0.0 0.0 0.44325871068232625 9.822000790671063E-4 60-61 2.4555001976677657E-4 0.0 0.0 0.5756101713366188 0.001064050085656032 62-63 2.4555001976677657E-4 0.0 0.0 0.7037054316482872 0.001064050085656032 64-65 2.4555001976677657E-4 0.0 0.0 0.850748968485292 0.001064050085656032 66-67 3.274000263557021E-4 0.0 0.0 1.041173008814427 0.001064050085656032 68-69 3.274000263557021E-4 0.0 0.0 1.2746501526093375 0.001064050085656032 70-71 3.274000263557021E-4 0.0 0.0 1.4013539628089942 0.001064050085656032 72-73 3.274000263557021E-4 0.0 0.0 1.5619846007397604 0.0011459000922449574 74-75 3.274000263557021E-4 0.0 0.0 1.7410724151563293 0.001227750098833883 76-77 3.274000263557021E-4 0.0 0.0 1.9307188804228699 0.001227750098833883 78-79 3.274000263557021E-4 0.0 0.0 2.138781597171919 0.001227750098833883 80-81 4.0925003294462766E-4 0.0 0.0 2.441708471557532 0.001227750098833883 82-83 4.0925003294462766E-4 0.0 0.0 2.7475000961737575 0.001227750098833883 84-85 4.5017503623909043E-4 0.0 0.0 3.221820884356581 0.001227750098833883 86-87 4.911000395335531E-4 0.0 0.0 3.8363507338262344 0.0012686751021283456 88-89 4.911000395335531E-4 0.0 0.0 4.548282091136708 0.0013505251087172713 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 355 0.0 36.14967 9 GTATCAA 8220 0.0 35.449566 1 GGTATCA 5075 0.0 33.720093 1 ATCAACG 9895 0.0 29.113667 3 TCAACGC 9885 0.0 29.09503 4 TATCAAC 10020 0.0 28.797916 2 CAACGCA 9965 0.0 28.76604 5 AACGCAG 10255 0.0 27.951424 6 ACGCAGA 11430 0.0 24.867025 7 CGCAGAG 11315 0.0 24.783712 8 GAGTACT 5560 0.0 23.6378 12-13 GCAGAGT 11795 0.0 23.412462 9 CATGGGG 2325 0.0 23.308914 4 TACATGG 6835 0.0 22.53443 2 ACATGGG 6750 0.0 22.254786 3 GTACATG 7015 0.0 22.226366 1 AGTACTT 5920 0.0 22.079931 12-13 GTACTTT 5975 0.0 22.033981 14-15 AGAGTAC 9415 0.0 21.758345 10-11 GTGTAGC 940 0.0 21.745174 1 >>END_MODULE