FastQCFastQC Report
Thu 26 May 2016
SRR937100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221747
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.6338464510246393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.5025590404560027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.3161865754530193No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16810.1375898610759838No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.11328040911907293No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT12910.10566835850630285No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC12530.10255805825592368No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12350.10108475813732302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.035.5926971
GTATCAA89700.032.9457051
TCAACGC111750.026.2695664
ATCAACG111650.026.250553
TATCAAC111700.026.2398722
CAACGCA113750.025.8076845
AACGCAG117150.025.0597046
GAGTACT70700.023.04741312-13
GTACATG66700.022.5805361
CGCAGAG130550.022.4538768
ACGCAGA131400.022.342047
TACATGG67750.021.8763582
AGTACTT75200.021.76300612-13
TACTCCG1754.046671E-621.7160917
ACATGGG65500.021.3940333
GTACTTT77100.020.91561714-15
GCAGAGT137100.020.8266819
AGAGTAC110450.020.5395410-11
GTATAGG3801.0913936E-1118.754631
CATGGGG24700.018.6546344
Produced by FastQC (version 0.11.5)