##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937100_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1221747 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04680674476794 31.0 31.0 34.0 30.0 34.0 2 32.1840397398152 33.0 31.0 34.0 30.0 34.0 3 32.290265496866375 34.0 31.0 34.0 30.0 34.0 4 35.99065109224741 37.0 35.0 37.0 35.0 37.0 5 35.82432942335852 37.0 35.0 37.0 35.0 37.0 6 35.91448884261635 37.0 35.0 37.0 35.0 37.0 7 35.86998617553389 37.0 35.0 37.0 35.0 37.0 8 35.83454103018055 37.0 35.0 37.0 35.0 37.0 9 37.49785020957694 39.0 37.0 39.0 35.0 39.0 10-11 37.414472881865066 39.0 37.0 39.0 34.0 39.0 12-13 37.32545404244905 39.0 37.0 39.0 34.0 39.0 14-15 38.48318309764623 40.0 38.0 41.0 34.0 41.0 16-17 38.47703329740118 40.0 38.0 41.0 34.0 41.0 18-19 38.63269523068196 40.0 38.0 41.0 34.0 41.0 20-21 38.65539346525917 40.0 38.0 41.0 34.0 41.0 22-23 38.55651088154913 40.0 38.0 41.0 34.0 41.0 24-25 38.459900863272026 40.0 38.0 41.0 34.0 41.0 26-27 38.25381768893232 40.0 38.0 41.0 34.0 41.0 28-29 38.10313223605215 40.0 37.5 41.0 33.0 41.0 30-31 38.025968551590466 40.0 37.0 41.0 33.0 41.0 32-33 37.842665052584536 40.0 37.0 41.0 33.0 41.0 34-35 37.69538415072842 40.0 37.0 41.0 33.0 41.0 36-37 37.519330925306136 40.0 37.0 41.0 33.0 41.0 38-39 37.332922037050224 40.0 36.0 41.0 32.5 41.0 40-41 36.94582020663853 39.5 36.0 41.0 31.5 41.0 42-43 36.73440573211966 39.0 36.0 41.0 31.0 41.0 44-45 36.45219959615207 39.0 36.0 41.0 30.5 41.0 46-47 35.986650673175376 39.0 35.0 40.5 29.5 41.0 48-49 35.54171976685844 39.0 35.0 40.0 27.5 41.0 50-51 35.260510563971096 39.0 35.0 40.0 26.5 41.0 52-53 35.03209788933388 39.0 35.0 40.0 26.0 41.0 54-55 34.66949294739418 38.0 34.0 40.0 24.0 41.0 56-57 34.48963083191528 38.0 34.0 40.0 24.0 41.0 58-59 34.19100517537592 38.0 34.0 40.0 23.0 41.0 60-61 33.839126267549666 38.0 33.0 40.0 22.5 41.0 62-63 33.490672373249126 37.0 33.0 40.0 22.0 41.0 64-65 33.20564936930477 37.0 33.0 39.0 21.0 40.0 66-67 32.887342060181034 36.0 32.0 39.0 20.0 40.0 68-69 32.42445940116898 36.0 31.5 39.0 18.5 40.0 70-71 32.000435442035055 35.0 31.0 38.0 17.5 40.0 72-73 31.586779013985712 35.0 31.0 38.0 13.5 39.5 74-75 31.09444385785273 35.0 30.5 37.0 10.0 39.0 76-77 29.624146815993818 33.5 28.5 35.5 8.0 38.0 78-79 30.293586151633683 34.0 30.0 36.0 7.0 38.5 80-81 30.144190245607316 34.0 30.0 36.0 6.5 37.5 82-83 29.777886501869865 34.0 30.0 35.0 2.0 37.0 84-85 29.32801226440499 34.0 29.0 35.0 2.0 36.5 86-87 28.965186736697532 34.0 29.0 35.0 2.0 36.0 88-89 28.57330445665101 34.0 29.0 35.0 2.0 36.0 90-91 28.112520431807894 33.0 28.5 35.0 2.0 35.0 92-93 27.626750873953448 33.0 27.0 35.0 2.0 35.0 94-95 27.081358088049328 33.0 26.0 34.5 2.0 35.0 96-97 26.330601180113394 32.0 25.0 34.0 2.0 35.0 98-99 25.520828371176684 32.0 23.5 34.0 2.0 35.0 100-101 22.525766382074195 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17160538718669827 1101 2 0.3347742026242173 1101 3 0.3285029427511361 1101 4 0.1544085125149408 1101 5 -0.0016751102305363474 1101 6 0.06085015881733824 1101 7 0.09205755474346944 1101 8 0.15720190459006034 1101 9 0.1600081224655483 1101 10-11 0.26412188836081185 1101 12-13 0.28386249695948607 1101 14-15 0.43985532145054407 1101 16-17 0.28556693016400914 1101 18-19 0.16987391295841547 1101 20-21 0.1895736973286617 1101 22-23 0.29222357895017126 1101 24-25 0.13165255800118558 1101 26-27 0.20244247103639879 1101 28-29 0.24603758215519633 1101 30-31 0.3715605508594919 1101 32-33 0.4340095811266522 1101 34-35 0.2544512363117235 1101 36-37 0.28763262108164867 1101 38-39 0.3124053593544929 1101 40-41 0.5880624167484427 1101 42-43 0.48846989845031885 1101 44-45 0.2588099096009273 1101 46-47 0.16751252526658078 1101 48-49 0.41457252583520443 1101 50-51 0.4460488795552422 1101 52-53 0.5047604905367962 1101 54-55 0.31674140370419224 1101 56-57 0.5864230120307994 1101 58-59 0.513209640494587 1101 60-61 0.592807100273113 1101 62-63 0.5584815963460841 1101 64-65 0.5293469170772056 1101 66-67 0.4733098229707906 1101 68-69 0.18962490301237978 1101 70-71 0.2026255736009972 1101 72-73 0.3209829112086737 1101 74-75 0.2653171290344325 1101 76-77 0.1914581157919919 1101 78-79 0.2531448201023778 1101 80-81 0.31374025700138475 1101 82-83 0.679621217321273 1101 84-85 0.25923101477229693 1101 86-87 -0.06877710752293709 1101 88-89 0.19350793941544353 1101 90-91 0.3508401367090066 1101 92-93 0.43811988824642256 1101 94-95 0.34110049269553144 1101 96-97 0.26094007850190337 1101 98-99 0.600593251739685 1101 100-101 0.7876677737854614 1102 1 -0.05732473154074924 1102 2 -0.02633097857381017 1102 3 0.008719354929233702 1102 4 0.06227799428077674 1102 5 -0.009730526539023288 1102 6 0.1440470512040264 1102 7 0.030891666060220757 1102 8 0.20484536771859752 1102 9 0.23645857391194625 1102 10-11 0.22388459547625672 1102 12-13 0.23486884027222743 1102 14-15 0.27752569791319814 1102 16-17 0.20234478270104717 1102 18-19 0.34487896397367734 1102 20-21 0.2239820709589324 1102 22-23 0.06122492652291811 1102 24-25 0.10845314963707864 1102 26-27 0.20651830678882277 1102 28-29 0.07336386058621258 1102 30-31 0.15843415349136336 1102 32-33 0.14225817494305915 1102 34-35 0.29981888351070296 1102 36-37 0.23133190841199536 1102 38-39 0.38515641863816086 1102 40-41 0.35708639858766134 1102 42-43 0.395486187497994 1102 44-45 0.5068128525320788 1102 46-47 0.47697822315486604 1102 48-49 0.5963435547365421 1102 50-51 0.5323454751709633 1102 52-53 0.5418868821868017 1102 54-55 0.7746049767351977 1102 56-57 0.5932125772051009 1102 58-59 0.25915620075320334 1102 60-61 0.428307684475449 1102 62-63 0.38772916661714163 1102 64-65 0.6031879949829246 1102 66-67 0.4975854311416157 1102 68-69 0.5276057263088134 1102 70-71 0.5695463317488567 1102 72-73 0.3068784276225678 1102 74-75 0.3531759655473614 1102 76-77 0.44885832965006855 1102 78-79 0.04962488193443093 1102 80-81 0.41231844385845307 1102 82-83 0.5495120677407002 1102 84-85 0.46097428215016834 1102 86-87 0.42079874061876765 1102 88-89 0.38024087603759327 1102 90-91 0.43229585836566287 1102 92-93 0.33225680316254724 1102 94-95 0.21924117585963998 1102 96-97 0.4618525491726615 1102 98-99 0.3649479035341301 1102 100-101 0.261671484852144 1103 1 0.18991489804760064 1103 2 0.23743588938470594 1103 3 0.2286518955895005 1103 4 0.10404950912727884 1103 5 0.11886777553268502 1103 6 0.12650677326035265 1103 7 0.08150866063618878 1103 8 0.10618121057802199 1103 9 0.28149210617154097 1103 10-11 0.27104778480671143 1103 12-13 0.16400267394482881 1103 14-15 0.1726586100046532 1103 16-17 0.1602407939639363 1103 18-19 0.19027535631335724 1103 20-21 0.21296107999932445 1103 22-23 0.214781385112488 1103 24-25 0.19318688815292262 1103 26-27 0.1681858538886658 1103 28-29 0.06439481201879005 1103 30-31 0.2697455624559879 1103 32-33 0.2923029795793539 1103 34-35 0.25092785448338617 1103 36-37 0.3105365349092395 1103 38-39 0.27955254060073287 1103 40-41 0.42198640153632283 1103 42-43 0.2218036262220977 1103 44-45 0.2127847383099919 1103 46-47 0.2379388665424571 1103 48-49 0.12711781783395537 1103 50-51 0.00879767764493522 1103 52-53 0.03612462613217815 1103 54-55 0.4467853457574833 1103 56-57 0.21508356184258304 1103 58-59 0.17473906833988195 1103 60-61 0.0914606564496836 1103 62-63 0.35168130037234846 1103 64-65 0.06700918825058011 1103 66-67 0.23197640044606516 1103 68-69 0.2714766369799122 1103 70-71 0.10928964341370673 1103 72-73 0.1632007291365305 1103 74-75 0.08223011043851258 1103 76-77 0.2759048120238283 1103 78-79 0.277681105380875 1103 80-81 0.377785389131823 1103 82-83 0.5485380104497359 1103 84-85 0.40910635612254964 1103 86-87 0.15979474193617094 1103 88-89 0.33211799788571383 1103 90-91 -0.014761079256189191 1103 92-93 -0.03530164152434523 1103 94-95 0.3568191466719064 1103 96-97 0.36443320126628365 1103 98-99 0.4459437159373927 1103 100-101 0.7687010399193639 1104 1 -0.021509958759740044 1104 2 0.047770978720798496 1104 3 0.024371693322386534 1104 4 0.0152000722028518 1104 5 -0.1535672839557236 1104 6 0.007632902625033466 1104 7 0.04106504117898169 1104 8 0.06770846456878843 1104 9 0.011609895612430421 1104 10-11 0.16975000943998708 1104 12-13 0.11122890853895484 1104 14-15 0.08536460263641032 1104 16-17 0.0375086464771357 1104 18-19 0.18218529799053584 1104 20-21 0.18190082797153195 1104 22-23 0.07842809740036216 1104 24-25 0.0841976137132221 1104 26-27 0.18133286247478253 1104 28-29 0.19478904662162932 1104 30-31 0.14101146942270049 1104 32-33 0.1962420303792456 1104 34-35 0.2273518561653205 1104 36-37 0.26495208664487535 1104 38-39 0.10096341038854462 1104 40-41 0.36982783622979554 1104 42-43 0.15676357694073317 1104 44-45 0.39164688228914457 1104 46-47 0.5244024763263937 1104 48-49 0.628753857938797 1104 50-51 0.5782699454375404 1104 52-53 0.4574686109512527 1104 54-55 0.6911878146189281 1104 56-57 0.5370190810289799 1104 58-59 0.42918354183317575 1104 60-61 0.4710907248379286 1104 62-63 0.5771712000884719 1104 64-65 0.5676582938002923 1104 66-67 0.18904338563333312 1104 68-69 0.36072599481885703 1104 70-71 0.33388642727068074 1104 72-73 0.2750033389575073 1104 74-75 0.1532937557346692 1104 76-77 0.3868226121401719 1104 78-79 0.49755699781590224 1104 80-81 0.5045612721986004 1104 82-83 0.6499953729161589 1104 84-85 0.47573056608470665 1104 86-87 0.4645280789732311 1104 88-89 0.592633725657354 1104 90-91 0.8935521831398638 1104 92-93 0.8334650661011977 1104 94-95 0.8192991574345037 1104 96-97 0.8746112361989731 1104 98-99 0.5820158198945009 1104 100-101 1.095358403692984 1105 1 -0.038828835811443696 1105 2 0.01961370342612412 1105 3 -0.027581442600215667 1105 4 -0.00466269861411206 1105 5 -0.07055519426424439 1105 6 -0.09570714155470483 1105 7 0.029154559802847757 1105 8 0.03511859896365621 1105 9 0.15152754793466272 1105 10-11 0.08793328926449107 1105 12-13 0.02944173896552371 1105 14-15 0.07996907577141599 1105 16-17 -0.19022942334109416 1105 18-19 -0.015560817217412648 1105 20-21 -0.018498486454497254 1105 22-23 0.06275356836705726 1105 24-25 -0.008719011646157071 1105 26-27 0.016715831922631708 1105 28-29 0.00735863352328181 1105 30-31 0.018271137235799984 1105 32-33 0.09030709381453761 1105 34-35 0.14274196859741295 1105 36-37 0.30908257447765664 1105 38-39 0.1980975103852458 1105 40-41 0.38723822482338477 1105 42-43 0.26616419540623326 1105 44-45 0.06329854487592712 1105 46-47 -0.04286788054319857 1105 48-49 0.03257093780549525 1105 50-51 0.21419175263796575 1105 52-53 0.39639577793185765 1105 54-55 0.48451916520219385 1105 56-57 0.3136846864159608 1105 58-59 0.23514284970087118 1105 60-61 0.30785621761487647 1105 62-63 0.16067818292751213 1105 64-65 0.2481185777911108 1105 66-67 0.1385140803330387 1105 68-69 0.06322340199182719 1105 70-71 -0.07325922605818036 1105 72-73 0.05729404443523478 1105 74-75 0.008111965514711272 1105 76-77 0.03537587185754987 1105 78-79 0.07428593046086363 1105 80-81 -0.0035368637621324694 1105 82-83 0.05114479601214583 1105 84-85 -0.2517037362541785 1105 86-87 -0.23745680001546532 1105 88-89 0.07208382390516377 1105 90-91 0.13513042042420764 1105 92-93 0.23357878825690293 1105 94-95 0.3353602274077865 1105 96-97 0.34849120483085017 1105 98-99 0.40141795084242915 1105 100-101 0.21828318883150644 1106 1 0.04031925870404862 1106 2 0.10796853098618442 1106 3 0.03135304166040953 1106 4 0.15126413578332887 1106 5 0.01929568458558606 1106 6 0.10576252646581707 1106 7 0.09773521272880004 1106 8 0.14875296711219477 1106 9 0.21772899533108614 1106 10-11 0.17493747332548537 1106 12-13 0.29226085981160566 1106 14-15 0.2256173568114832 1106 16-17 0.23751018578391836 1106 18-19 0.22332919238935034 1106 20-21 0.2761508066580589 1106 22-23 0.2630383449209006 1106 24-25 0.2377801726568336 1106 26-27 0.3970223061456224 1106 28-29 0.4066100939186015 1106 30-31 0.38046780994577745 1106 32-33 0.3242797931270829 1106 34-35 0.22043148805786217 1106 36-37 0.3569114584182529 1106 38-39 0.3049677393928789 1106 40-41 0.408465620764737 1106 42-43 0.40638763085910057 1106 44-45 0.2665077526368904 1106 46-47 0.32233352959114114 1106 48-49 0.052349181287951296 1106 50-51 0.06000488628079381 1106 52-53 0.21892460572234285 1106 54-55 0.5281965389361858 1106 56-57 0.37386380996889557 1106 58-59 0.3696786776237104 1106 60-61 0.10846615939608029 1106 62-63 0.237481121749596 1106 64-65 0.10245787605611412 1106 66-67 0.29162634242854324 1106 68-69 0.15624862499684156 1106 70-71 0.3312553967403069 1106 72-73 0.4087365368318423 1106 74-75 0.3149493867557247 1106 76-77 0.44707522422539014 1106 78-79 0.49277212186034447 1106 80-81 0.5525284399688317 1106 82-83 0.42126030419203175 1106 84-85 0.4666989795093528 1106 86-87 0.6557472809965894 1106 88-89 0.6466249113498499 1106 90-91 0.4353816611017294 1106 92-93 0.6250482549765088 1106 94-95 0.7739932142893942 1106 96-97 1.0260405417584764 1106 98-99 0.8342341977966825 1106 100-101 1.4541708166629448 1107 1 0.21588112533347115 1107 2 0.28635299953686655 1107 3 0.17946794458885051 1107 4 0.15419288789779984 1107 5 0.13562263959590126 1107 6 0.21347890059868746 1107 7 0.09942554592246466 1107 8 0.17378703139203822 1107 9 0.22728872033380298 1107 10-11 0.21809807980933726 1107 12-13 0.16533409413349887 1107 14-15 0.22885152351694416 1107 16-17 0.289743840361254 1107 18-19 0.18287448469035894 1107 20-21 0.30798469091709535 1107 22-23 0.25795927544218245 1107 24-25 0.3619290312318739 1107 26-27 0.3576368598957984 1107 28-29 0.27565738041549537 1107 30-31 0.12913456972665216 1107 32-33 -0.030487498956667025 1107 34-35 0.08295860756994244 1107 36-37 0.2168158611682216 1107 38-39 0.1862443351375589 1107 40-41 0.15159674108380017 1107 42-43 -0.11288204339638241 1107 44-45 -0.1631543979908585 1107 46-47 -0.3699355083839251 1107 48-49 -0.3348445117765948 1107 50-51 -0.570566058983573 1107 52-53 -0.24989299906992812 1107 54-55 -0.06701242485790004 1107 56-57 -0.0982841795402365 1107 58-59 -0.16682364247188275 1107 60-61 -0.375343142712957 1107 62-63 -0.3120705333553957 1107 64-65 -0.19249160948790944 1107 66-67 -0.24030127679642987 1107 68-69 -0.4531984318183433 1107 70-71 -0.7260629141680219 1107 72-73 -0.32916839356528627 1107 74-75 -0.2996195720722774 1107 76-77 -0.44966329556914175 1107 78-79 -0.2301408008393473 1107 80-81 -0.36474424497925995 1107 82-83 -0.18056970030848873 1107 84-85 -0.312080293268707 1107 86-87 -0.11350982284126943 1107 88-89 -0.2604939799703523 1107 90-91 -0.2296266415815147 1107 92-93 -0.2223093190880796 1107 94-95 0.22561697082678478 1107 96-97 0.4647173889115095 1107 98-99 0.5729085619042635 1107 100-101 1.0730953476001375 1108 1 0.03696778692640379 1108 2 0.03723880741880237 1108 3 0.08876479115249225 1108 4 0.10038063813770037 1108 5 -0.1921487776305284 1108 6 0.08570814516372849 1108 7 0.11698419719144937 1108 8 0.18836431209089 1108 9 0.141867986407064 1108 10-11 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-0.7163961625852977 2316 56-57 -0.6082320286208613 2316 58-59 -0.6357926212442564 2316 60-61 -0.656686824616834 2316 62-63 -0.6823190459524113 2316 64-65 -0.8022352620109885 2316 66-67 -0.9880553595033845 2316 68-69 -0.864954179437273 2316 70-71 -0.6997455525638294 2316 72-73 -0.8888035819696647 2316 74-75 -0.7587014220832948 2316 76-77 -0.6691633322951382 2316 78-79 -0.657759731867916 2316 80-81 -0.8203743407116981 2316 82-83 -0.8031321957177404 2316 84-85 -0.7187766671883509 2316 86-87 -0.5193032268517435 2316 88-89 -1.2343259707643703 2316 90-91 -1.2389927508291194 2316 92-93 -1.263968354908176 2316 94-95 -1.3268013138241948 2316 96-97 -1.260762619902728 2316 98-99 -1.4781758344427232 2316 100-101 -2.0867474651217464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 8.0 5 16.0 6 50.0 7 152.0 8 427.0 9 797.0 10 1487.0 11 2487.0 12 3965.0 13 5375.0 14 6686.0 15 9579.0 16 12403.0 17 17273.0 18 14592.0 19 8154.0 20 6570.0 21 6400.0 22 6940.0 23 7638.0 24 9047.0 25 10805.0 26 13048.0 27 15873.0 28 19738.0 29 24527.0 30 30727.0 31 37738.0 32 48015.0 33 62119.0 34 83457.0 35 116060.0 36 176988.0 37 271040.0 38 182390.0 39 9172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.733743544737386 21.439822379990243 15.803072328413634 21.02336174685874 2 14.687284880204107 22.534855034325034 41.070047302822374 21.70781278264848 3 18.048997291569982 27.54208952633987 32.511555231427934 21.89735795066221 4 11.91179515889951 19.33297155630421 40.1590509328036 28.596182351992677 5 11.67461044387748 39.694412180949506 35.46395178565144 13.16702558952158 6 27.389795104878505 40.127047580227334 18.843835916928793 13.639321397965373 7 25.009085373040506 36.096596340968325 22.394789743186788 16.499528542804384 8 25.18279152721472 35.353718895974374 21.227635508824658 18.235854067986253 9 26.449584079191517 17.000573768546186 22.334574997933288 34.215267154329005 10-11 23.21229354358963 28.32071615481765 29.182596724199033 19.284393577393683 12-13 24.72141332574801 26.689599990177282 29.10762631916065 19.48136036491406 14-15 21.992385116803696 27.360660856938342 28.657515655612137 21.989438370645825 16-17 19.50759854536168 30.771796452129614 29.114865843746703 20.605739158762002 18-19 19.65296344694001 28.707393403448922 32.65977460917565 18.97986854043542 20-21 20.820020667287366 27.59213824573609 32.604548849486896 18.98329223748964 22-23 20.405672639646923 27.66926081504497 32.498543042454756 19.426523502853346 24-25 20.09387465617558 28.032867998024123 32.7747699890852 19.0984873567151 26-27 20.0212237675093 28.01201725239381 32.8400764972353 19.126682482861582 28-29 19.559613323407635 28.346884311736208 32.83505675695696 19.258445607899198 30-31 20.158060547723874 27.893581895433346 32.60470457467871 19.343652982164063 32-33 20.04757134064092 27.886202019408323 32.60564406291334 19.460582577037417 34-35 20.229679303489185 28.01991738060335 32.422588310018355 19.327815005889107 36-37 20.64644609347759 27.57819674787964 32.281583595667115 19.493773562975658 38-39 19.856756897242576 28.47520463446569 32.09450983297309 19.573528635318645 40-41 20.310377638404322 28.079886227606178 32.03274025721565 19.576995876773857 42-43 20.243839354629067 28.211241771004964 31.72641307897625 19.818505795389715 44-45 20.662603612385933 28.1267879198619 31.48838419926973 19.722224268482435 46-47 20.828036947153457 28.532795305075936 30.841787770770495 19.79737997700011 48-49 20.5724755503699 28.706814384162342 30.71148556595006 20.0092244995177 50-51 20.407628724806614 28.983948508849583 30.301348626470208 20.307074139873595 52-53 20.84666811814606 29.268372536918598 29.894486724693103 19.990472620242244 54-55 20.876580830564755 28.921658903193542 29.759762045660842 20.441998220580857 56-57 21.0297717243307 29.37360573572341 29.52588773530352 20.07073480464237 58-59 20.95927506997161 28.96106431331239 29.793234351998844 20.28642626471716 60-61 20.858149750168103 29.104710034160814 29.98330634273032 20.053833872940764 62-63 20.62239563510285 28.90328357671433 30.241858584469618 20.232462203713208 64-65 20.546905821668336 28.93248678530562 30.034720801214 20.485886591812047 66-67 20.664302838476377 29.183865399301162 29.472877772566658 20.678953989655795 68-69 20.791178857479846 28.924208090630916 29.721795806249496 20.562817245639746 70-71 20.77095120678542 28.951882053223382 29.778205220024763 20.498961519966443 72-73 21.00481363040715 28.64572547819133 29.797567238174437 20.55189365322708 74-75 20.6258471884802 29.425106901271032 29.241262908369404 20.707783001879367 76-77 20.493695897012795 29.36245236317919 29.382382888723008 20.761468851085006 78-79 20.71262123048792 29.369883846275364 28.908862174778164 21.008632748458556 80-81 20.65966474081647 29.057146470340996 29.006643548824744 21.276545240017793 82-83 20.743288094834693 29.225486127651635 29.204778075984635 20.82644770152904 84-85 20.58612277171317 29.20026209240926 28.975167112559358 21.238448023318202 86-87 20.259948818918584 29.086127536903295 29.077860642255864 21.576063001922257 88-89 20.655878196504805 28.810625801389943 29.129196848176587 21.40429915392867 90-91 20.303392063399166 28.764376765697854 29.587274329336644 21.344956841566333 92-93 20.254070605452686 28.77355131627088 29.515030525959958 21.457347552316477 94-95 20.089142842176 28.733444813042308 29.564140529913313 21.61327181486838 96-97 20.380023417411472 28.76879337306081 29.27855792617172 21.572625283355997 98-99 20.039828783703758 28.890069364781645 28.949002213665135 22.121099637849458 100-101 19.837711542026835 28.8693298387879 28.991166914337935 22.301791704847332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 594.0 1 420.5 2 265.0 3 274.0 4 243.0 5 263.0 6 403.5 7 720.5 8 1182.0 9 1509.0 10 1443.0 11 1291.5 12 1364.5 13 1517.0 14 1692.0 15 1897.0 16 2135.5 17 2386.0 18 2612.5 19 2857.5 20 3238.0 21 3779.0 22 4454.5 23 5398.0 24 6524.5 25 8503.5 26 12425.5 27 16766.5 28 18290.5 29 19932.5 30 23409.0 31 26929.5 32 30410.5 33 33606.0 34 38710.5 35 43884.0 36 49019.5 37 52433.5 38 49941.5 39 46803.0 40 45829.0 41 45213.5 42 43973.5 43 42921.0 44 42903.0 45 43198.5 46 42115.0 47 40654.5 48 41455.5 49 39813.0 50 35047.0 51 31356.5 52 28823.0 53 26573.0 54 24688.5 55 22153.0 56 19079.0 57 16559.0 58 14898.5 59 13517.5 60 11182.5 61 8780.0 62 6725.0 63 4678.5 64 3019.5 65 2098.0 66 1488.5 67 1073.0 68 836.5 69 610.0 70 411.5 71 279.0 72 181.0 73 126.0 74 90.0 75 59.0 76 46.0 77 29.5 78 13.5 79 5.0 80 2.0 81 3.5 82 2.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021526551732887415 2 0.003601400289912723 3 4.911000395335531E-4 4 0.0 5 0.002946600237201319 6 0.0 7 2.4555001976677657E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.007325575589708835 14-15 0.004951925398629995 16-17 0.0 18-19 2.046250164723138E-4 20-21 7.775750625947925E-4 22-23 0.0018825501515452871 24-25 9.412750757726436E-4 26-27 2.8647502306123934E-4 28-29 2.046250164723138E-4 30-31 0.0 32-33 2.4555001976677657E-4 34-35 0.0 36-37 0.001718850138367436 38-39 0.00961737577419875 40-41 7.775750625947925E-4 42-43 0.0 44-45 4.911000395335531E-4 46-47 1.6370001317785106E-4 48-49 4.0925003294462764E-5 50-51 0.0018416251482508243 52-53 4.092500329446276E-4 54-55 0.0 56-57 4.0925003294462764E-5 58-59 1.2277500988338829E-4 60-61 0.002087175168017601 62-63 0.0 64-65 8.185000658892553E-5 66-67 0.0 68-69 4.0925003294462764E-5 70-71 7.775750625947925E-4 72-73 6.548000527114042E-4 74-75 0.004829150388746606 76-77 2.4555001976677657E-4 78-79 3.274000263557021E-4 80-81 0.0029056752339068565 82-83 0.0 84-85 0.003314925266851484 86-87 5.32025042828016E-4 88-89 0.0033967752734404095 90-91 0.002250875181195452 92-93 0.0 94-95 0.0 96-97 5.32025042828016E-4 98-99 0.0014323751153061967 100-101 0.002741975220729005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1221747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.73395103801717 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82283310914546 40.742604018505695 2 14.048846594487127 15.09800070097571 3 4.634768997038373 7.471333510781397 4 1.9821400731655086 4.260328705678688 5 1.021760043261288 2.7451602068602177 6 0.5805032930023906 1.8715641314158915 7 0.3763364357597381 1.4155430529054964 8 0.2357072036810544 1.0132383473524575 9 0.18295601808850728 0.8847854746270591 >10 0.9336680013367518 9.563033788462182 >50 0.10269129131822238 3.8443279448229504 >100 0.07093864876274135 6.983877788754896 >500 0.005175775386736503 1.8120819177907839 >1k 0.0013700581906067213 1.1584301954477083 >5k 3.044573756903825E-4 1.135690215618979 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7744 0.6338464510246393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6140 0.5025590404560027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3863 0.3161865754530193 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1681 0.1375898610759838 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.11328040911907293 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1291 0.10566835850630285 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1253 0.10255805825592368 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1235 0.10108475813732302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 4.0925003294462764E-5 0.0 0.0 0.0 2.046250164723138E-4 14-15 8.185000658892553E-5 0.0 0.0 0.0 2.8647502306123934E-4 16-17 8.185000658892553E-5 0.0 0.0 0.0 3.274000263557021E-4 18-19 8.185000658892553E-5 0.0 0.0 8.185000658892553E-5 3.274000263557021E-4 20-21 8.185000658892553E-5 0.0 0.0 1.2277500988338829E-4 4.911000395335531E-4 22-23 8.185000658892553E-5 0.0 0.0 3.683250296501649E-4 4.911000395335531E-4 24-25 8.185000658892553E-5 0.0 0.0 8.185000658892553E-4 4.911000395335531E-4 26-27 8.185000658892553E-5 0.0 0.0 0.0014323751153061967 4.911000395335531E-4 28-29 8.185000658892553E-5 0.0 0.0 0.0031921502569680956 4.911000395335531E-4 30-31 8.185000658892553E-5 0.0 0.0 0.0064661505205251164 4.911000395335531E-4 32-33 8.185000658892553E-5 0.0 0.0 0.010435875840088005 4.911000395335531E-4 34-35 8.185000658892553E-5 0.0 0.0 0.015060401212362299 4.911000395335531E-4 36-37 1.6370001317785106E-4 0.0 0.0 0.020748976670292622 4.911000395335531E-4 38-39 2.4555001976677657E-4 0.0 0.0 0.030161727428019057 4.911000395335531E-4 40-41 2.4555001976677657E-4 0.0 0.0 0.04072037827799045 4.911000395335531E-4 42-43 2.4555001976677657E-4 0.0 0.0 0.05803165467154821 4.911000395335531E-4 44-45 3.274000263557021E-4 0.0 0.0 0.07325575589708835 4.911000395335531E-4 46-47 4.0925003294462766E-4 0.0 0.0 0.10116660814391196 4.911000395335531E-4 48-49 4.0925003294462766E-4 0.0 0.0 0.14041368630330175 4.911000395335531E-4 50-51 4.0925003294462766E-4 0.0 0.0 0.15928011282204907 4.911000395335531E-4 52-53 4.0925003294462766E-4 0.0 0.0 0.19058774034231307 5.729500461224787E-4 54-55 4.0925003294462766E-4 0.0 0.0 0.24452689468441502 5.729500461224787E-4 56-57 4.0925003294462766E-4 0.0 0.0 0.32911887649406957 6.548000527114042E-4 58-59 4.0925003294462766E-4 0.0 0.0 0.4471465859953002 7.366500593003298E-4 60-61 4.0925003294462766E-4 0.0 0.0 0.5800300716924207 7.366500593003298E-4 62-63 4.0925003294462766E-4 0.0 0.0 0.708370882023856 7.366500593003298E-4 64-65 4.0925003294462766E-4 0.0 0.0 0.8561919939234555 7.366500593003298E-4 66-67 4.0925003294462766E-4 0.0 0.0 1.0468615842723574 7.366500593003298E-4 68-69 4.911000395335531E-4 0.0 0.0 1.2810344531232736 7.366500593003298E-4 70-71 4.911000395335531E-4 0.0 0.0 1.4085567633888194 7.775750625947926E-4 72-73 4.911000395335531E-4 0.0 0.0 1.5699649763821806 8.59425069183718E-4 74-75 4.911000395335531E-4 0.0 0.0 1.7501577658877001 9.003500724781809E-4 76-77 4.911000395335531E-4 0.0 0.0 1.939845156157535 9.003500724781809E-4 78-79 4.911000395335531E-4 0.0 0.0 2.1481534229263506 9.412750757726436E-4 80-81 4.911000395335531E-4 0.0 0.0 2.4533311724931592 9.822000790671063E-4 82-83 4.911000395335531E-4 0.0 0.0 2.760432397214808 9.822000790671063E-4 84-85 5.320250428280159E-4 0.0 0.0 3.238600135707311 9.822000790671063E-4 86-87 5.729500461224787E-4 0.0 0.0 3.8557491853878094 0.0010231250823615691 88-89 5.729500461224787E-4 0.0 0.0 4.569563092849829 0.001064050085656032 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5780 0.0 35.592697 1 GTATCAA 8970 0.0 32.945705 1 TCAACGC 11175 0.0 26.269566 4 ATCAACG 11165 0.0 26.25055 3 TATCAAC 11170 0.0 26.239872 2 CAACGCA 11375 0.0 25.807684 5 AACGCAG 11715 0.0 25.059704 6 GAGTACT 7070 0.0 23.047413 12-13 GTACATG 6670 0.0 22.580536 1 CGCAGAG 13055 0.0 22.453876 8 ACGCAGA 13140 0.0 22.34204 7 TACATGG 6775 0.0 21.876358 2 AGTACTT 7520 0.0 21.763006 12-13 TACTCCG 175 4.046671E-6 21.716091 7 ACATGGG 6550 0.0 21.394033 3 GTACTTT 7710 0.0 20.915617 14-15 GCAGAGT 13710 0.0 20.826681 9 AGAGTAC 11045 0.0 20.53954 10-11 GTATAGG 380 1.0913936E-11 18.75463 1 CATGGGG 2470 0.0 18.654634 4 >>END_MODULE