FastQCFastQC Report
Thu 26 May 2016
SRR937099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846310
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209761.1361039045447405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148450.804036158608251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102240.5537531617117386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35640.19303367256852857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32070.17369780806040155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.13383451316409486No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.1292307359002551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91450.048.3315471
GTATCAA166450.043.0575261
TATCAAC212850.033.5372732
ATCAACG213400.033.4490243
TCAACGC214900.033.2385674
CAACGCA218050.032.778415
AACGCAG223350.032.0635576
ACGCAGA253050.028.1868767
CGCAGAG253600.028.0125568
GTACATG154600.026.6834351
GCAGAGT261800.026.2639319
TACATGG160200.025.483682
GAGTACT141350.024.90412912-13
ACATGGG159150.024.6948743
CGCAGTA1000.001648758123.7630142
AGTACTT143850.023.43103612-13
GTACTTT151950.022.90169514-15
CATGGGA107950.022.8488544
CATGGGG49300.022.0753844
AGAGTAC235950.021.80950410-11
Produced by FastQC (version 0.11.5)