FastQCFastQC Report
Thu 26 May 2016
SRR937099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846310
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206671.1193678201385466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163250.8841960450845199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113950.6171769637818135No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.1987206915415071No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.1969875048068851No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31520.17071889336026994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.16551933315640385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.049.6176871
GTATCAA165100.041.8655781
TATCAAC219850.031.4345382
ATCAACG222250.031.0737193
TCAACGC224000.030.8513224
CAACGCA228500.030.2229635
AACGCAG235450.029.3510156
ACGCAGA267200.025.721187
CGCAGAG268600.025.427978
GCAGAGT277250.024.0350469
GTACATG146050.023.1915151
GAGTACT161450.022.8287712-13
GTGGTAT44650.022.5556621
AGTACTT164750.022.45798912-13
CATATAG4050.022.2827683
TACATGG151350.022.2816072
TGGTATC46600.021.4044442
AGAGTAC253000.021.23248910-11
GTACTTT172750.021.13156714-15
ACATGGG150000.021.1205463
Produced by FastQC (version 0.11.5)