##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937099_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846310 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.665688860483883 31.0 31.0 34.0 30.0 34.0 2 31.62702742226387 31.0 31.0 34.0 30.0 34.0 3 31.78572612399868 31.0 31.0 34.0 30.0 34.0 4 35.65156555508013 37.0 35.0 37.0 33.0 37.0 5 35.55049856199663 37.0 35.0 37.0 33.0 37.0 6 35.606665727857184 37.0 35.0 37.0 33.0 37.0 7 35.515699963711405 37.0 35.0 37.0 33.0 37.0 8 35.500606073736265 37.0 35.0 37.0 33.0 37.0 9 37.06365615741669 39.0 37.0 39.0 33.0 39.0 10-11 36.978357372272264 39.0 37.0 39.0 33.0 39.0 12-13 36.84530414719089 39.0 37.0 39.0 32.0 39.0 14-15 37.8194011298211 40.0 37.0 41.0 32.5 41.0 16-17 37.74787657543966 39.0 37.0 41.0 32.5 41.0 18-19 37.88944462197573 39.5 37.0 41.0 32.5 41.0 20-21 38.00005253722289 40.0 37.0 41.0 33.0 41.0 22-23 37.90887960310024 40.0 37.0 41.0 33.0 41.0 24-25 37.77374330421219 40.0 37.0 41.0 32.0 41.0 26-27 37.54160298108118 39.0 36.5 41.0 32.0 41.0 28-29 37.306538988577216 39.0 36.0 41.0 31.5 41.0 30-31 37.02223759823649 39.0 36.0 40.0 31.0 41.0 32-33 36.83966560328439 39.0 35.0 41.0 31.0 41.0 34-35 36.666361824395686 39.0 35.0 41.0 30.5 41.0 36-37 36.40540781342244 39.0 35.0 40.0 30.0 41.0 38-39 36.16335989080923 38.0 35.0 40.0 30.0 41.0 40-41 35.862168866550036 38.0 35.0 40.0 29.5 41.0 42-43 35.49383310494987 38.0 35.0 40.0 27.5 41.0 44-45 34.96195790522718 38.0 34.0 40.0 25.5 41.0 46-47 34.420402857591625 38.0 33.5 40.0 23.5 41.0 48-49 33.875544464364054 38.0 33.0 40.0 21.0 41.0 50-51 33.50919943021486 38.0 33.0 40.0 18.5 41.0 52-53 33.18390790278988 37.5 33.0 40.0 16.0 41.0 54-55 32.88574589315987 37.0 32.0 40.0 13.5 41.0 56-57 32.5836102821303 37.0 32.0 40.0 11.0 41.0 58-59 32.19642801046412 36.0 31.0 39.0 8.0 41.0 60-61 31.824866355054134 36.0 31.0 39.0 7.5 40.0 62-63 31.41298779728215 35.0 30.5 39.0 6.0 40.0 64-65 30.980089204954748 35.0 30.0 38.0 2.0 40.0 66-67 30.5816271373713 35.0 29.0 38.0 2.0 40.0 68-69 30.124203140317714 34.5 29.0 37.5 2.0 39.5 70-71 29.623963202279143 34.0 28.5 37.0 2.0 39.0 72-73 29.2746545813 34.0 28.0 36.0 2.0 39.0 74-75 28.820956664915425 34.0 27.5 36.0 2.0 38.5 76-77 27.515232544913907 31.5 26.0 34.5 2.0 36.5 78-79 28.23751022309363 33.5 27.0 35.0 2.0 37.0 80-81 28.073039467911673 33.5 27.0 35.0 2.0 37.0 82-83 27.726714636220354 33.0 26.0 35.0 2.0 36.0 84-85 27.31948101889715 33.0 26.0 35.0 2.0 36.0 86-87 26.905937247807792 33.0 25.0 35.0 2.0 35.5 88-89 26.485455313571393 33.0 25.0 35.0 2.0 35.0 90-91 25.989274823837818 32.0 23.5 34.5 2.0 35.0 92-93 25.42838012034815 32.0 21.0 34.0 2.0 35.0 94-95 24.844579729297898 32.0 18.5 34.0 2.0 35.0 96-97 24.08815637677313 31.0 6.5 34.0 2.0 35.0 98-99 23.19460843520319 31.0 2.0 34.0 2.0 35.0 100-101 20.29063158407851 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.394515909577148 1101 2 0.3461886621264476 1101 3 0.2807709942529577 1101 4 0.29005431291174943 1101 5 0.33892522721875906 1101 6 0.3763307490993171 1101 7 0.4096084821795358 1101 8 0.3811291223305844 1101 9 0.5123696792888168 1101 10-11 0.524778792992457 1101 12-13 0.4572790925789505 1101 14-15 0.5787718223569343 1101 16-17 0.6032937543378978 1101 18-19 0.6012340188072471 1101 20-21 0.6407552674962531 1101 22-23 0.5520567186704923 1101 24-25 0.5642459862247691 1101 26-27 0.44866994552018724 1101 28-29 0.32668069063301886 1101 30-31 0.4210130180228333 1101 32-33 0.555282449448292 1101 34-35 0.5705620646920195 1101 36-37 0.6577664933531437 1101 38-39 0.4682659309504231 1101 40-41 0.5565086450277263 1101 42-43 0.8116846635103343 1101 44-45 0.6427299369862354 1101 46-47 0.7304233513982723 1101 48-49 0.5736841609139276 1101 50-51 0.5265514465641488 1101 52-53 0.26728249572907714 1101 54-55 0.34313305239569303 1101 56-57 0.5102792903054123 1101 58-59 0.5029968783795766 1101 60-61 0.4910324082203026 1101 62-63 0.5287983764379014 1101 64-65 0.40293522979872876 1101 66-67 0.3250687327959234 1101 68-69 0.36960853163033036 1101 70-71 0.40675339272867106 1101 72-73 0.6169773014972719 1101 74-75 0.42715389516501645 1101 76-77 0.39951910000343105 1101 78-79 0.4430775846321673 1101 80-81 0.5827256188088015 1101 82-83 0.6549987391330525 1101 84-85 0.7210236876939575 1101 86-87 0.34035604500052585 1101 88-89 0.46793662276388304 1101 90-91 0.5945225033190908 1101 92-93 0.8479015546109103 1101 94-95 0.8729260874581826 1101 96-97 0.8159581460521927 1101 98-99 0.9305436468396309 1101 100-101 1.2051859473971085 1102 1 0.38107403051133204 1102 2 0.20264221803509486 1102 3 0.2867817080413566 1102 4 0.28446410932394883 1102 5 0.3638275462034102 1102 6 0.37075222635042593 1102 7 0.4279169676020018 1102 8 0.5352070722572861 1102 9 0.5369928877017287 1102 10-11 0.5762573259159822 1102 12-13 0.5635373786740487 1102 14-15 0.7546554393963234 1102 16-17 0.6970334087264689 1102 18-19 0.6843746951462748 1102 20-21 0.5678648346873629 1102 22-23 0.6780548540397362 1102 24-25 0.6707643246807677 1102 26-27 0.6565450748931667 1102 28-29 0.46441069173040006 1102 30-31 0.5885854107904223 1102 32-33 0.5345165306934447 1102 34-35 0.4845866331849251 1102 36-37 0.5749542637607234 1102 38-39 0.6797555114884233 1102 40-41 0.7592718704270354 1102 42-43 0.8370771166432789 1102 44-45 0.9980028059939272 1102 46-47 0.8493882384891407 1102 48-49 0.7464761980245669 1102 50-51 0.8939468746223156 1102 52-53 0.9254004789646118 1102 54-55 0.8242970848794826 1102 56-57 0.8404935322604601 1102 58-59 1.0229615838026476 1102 60-61 1.0114233105681798 1102 62-63 1.0708607699463073 1102 64-65 0.8886936743536289 1102 66-67 0.7481513307743413 1102 68-69 0.6522897513927148 1102 70-71 0.6792172597441635 1102 72-73 0.9387673531726861 1102 74-75 0.6919183986940141 1102 76-77 0.7137989870435746 1102 78-79 0.8796913586159256 1102 80-81 0.7262923506729955 1102 82-83 0.7821549682016808 1102 84-85 0.5831544387012002 1102 86-87 0.6129563933969173 1102 88-89 0.8442007277611019 1102 90-91 0.983146472830871 1102 92-93 1.1111510840729046 1102 94-95 1.1718907040454063 1102 96-97 1.1001583359580422 1102 98-99 1.2450891192696218 1102 100-101 1.268059292368246 1103 1 0.05263119776159897 1103 2 -0.1334740595042767 1103 3 0.04450450809538964 1103 4 0.21155685912220434 1103 5 0.22788842808673593 1103 6 0.29982351613336533 1103 7 0.45295827016535384 1103 8 0.35072402102825606 1103 9 0.2528719482545796 1103 10-11 0.3929501422139552 1103 12-13 0.350511831696501 1103 14-15 0.5143325347437653 1103 16-17 0.6540181311074349 1103 18-19 0.5524814098866955 1103 20-21 0.5285871359934546 1103 22-23 0.5530933857812315 1103 24-25 0.5101402143743528 1103 26-27 0.5318134982608953 1103 28-29 0.6725089381465139 1103 30-31 0.7098668812655902 1103 32-33 0.7757542395008485 1103 34-35 0.5878504015719273 1103 36-37 0.661038070731415 1103 38-39 0.6564057090826552 1103 40-41 0.640288450332342 1103 42-43 0.6183090413600496 1103 44-45 0.7151244069747946 1103 46-47 0.7696392229967799 1103 48-49 0.8307771543044637 1103 50-51 0.6642608953272813 1103 52-53 0.9069896665250141 1103 54-55 0.9111606184393324 1103 56-57 0.8798604167830035 1103 58-59 0.8697866548915485 1103 60-61 0.8773674854347497 1103 62-63 0.9834963237470049 1103 64-65 0.8597567694309163 1103 66-67 0.9833802526129212 1103 68-69 0.9114862823237431 1103 70-71 0.806071112745137 1103 72-73 0.8650154352444304 1103 74-75 0.8400764082741325 1103 76-77 0.7766269397872065 1103 78-79 0.9032100079788101 1103 80-81 1.0211333401334812 1103 82-83 1.0451574827225194 1103 84-85 0.9639545481486635 1103 86-87 0.7636263261698524 1103 88-89 0.9470025481910618 1103 90-91 1.3153151951010074 1103 92-93 1.4616665435271194 1103 94-95 1.4264712189333792 1103 96-97 1.5441699838004368 1103 98-99 1.6858450514260497 1103 100-101 1.6738071891412787 1104 1 0.3893701974592467 1104 2 0.15175299045767687 1104 3 0.2080230100009004 1104 4 0.12670758893158052 1104 5 0.06635434982618449 1104 6 0.17063496183043014 1104 7 0.3026039147334032 1104 8 0.291746186101733 1104 9 0.30157713051587365 1104 10-11 0.35745645349518185 1104 12-13 0.24465915251690973 1104 14-15 0.31593253341473115 1104 16-17 0.4081771973235533 1104 18-19 0.4483058137289504 1104 20-21 0.3218735708089966 1104 22-23 0.3286508352195483 1104 24-25 0.38425723307062754 1104 26-27 0.3892228161640645 1104 28-29 0.5621844529005315 1104 30-31 0.633163798888333 1104 32-33 0.5932815429772873 1104 34-35 0.41864236021218204 1104 36-37 0.4419071137225572 1104 38-39 0.35595906839873237 1104 40-41 0.3162260053629282 1104 42-43 0.5471576104817828 1104 44-45 0.5141385911598775 1104 46-47 0.5409748921188466 1104 48-49 0.5851094111149635 1104 50-51 0.6168320073022713 1104 52-53 0.5860043657133005 1104 54-55 0.5057932882491869 1104 56-57 0.6827591372492279 1104 58-59 0.6539085245857663 1104 60-61 0.6156976789098287 1104 62-63 0.7646893203679213 1104 64-65 0.5385999393254899 1104 66-67 0.7699546174146121 1104 68-69 0.6712771577959664 1104 70-71 0.6769153691303487 1104 72-73 0.7650005666034758 1104 74-75 0.6442526689521415 1104 76-77 0.70209891530628 1104 78-79 0.6526538875810282 1104 80-81 0.878126502219942 1104 82-83 0.7679457931465166 1104 84-85 0.8389225681108528 1104 86-87 0.8336281369875742 1104 88-89 0.9622026186302115 1104 90-91 1.183475204935906 1104 92-93 1.3116257585957847 1104 94-95 1.2136876764422908 1104 96-97 1.160181516549656 1104 98-99 1.1367249593235371 1104 100-101 1.0422778622006312 1105 1 0.2880659144342026 1105 2 0.14468724655788634 1105 3 0.2618694380053235 1105 4 0.1884297738932048 1105 5 0.2592937900099628 1105 6 0.16673150890676425 1105 7 0.1947393359563918 1105 8 0.21210327225796277 1105 9 0.2510784864777307 1105 10-11 0.3206198946898553 1105 12-13 0.3819106000102508 1105 14-15 0.5448790790330449 1105 16-17 0.5311857802219109 1105 18-19 0.4060587727066505 1105 20-21 0.2222480398454394 1105 22-23 0.4474044195273237 1105 24-25 0.31744857111499414 1105 26-27 0.33427720044074505 1105 28-29 0.4221149699804627 1105 30-31 0.40567730180600137 1105 32-33 0.519152388015307 1105 34-35 0.5664195223800377 1105 36-37 0.47859949020367054 1105 38-39 0.5034883665710126 1105 40-41 0.2933261262168756 1105 42-43 0.5918241605065688 1105 44-45 0.6894027364178967 1105 46-47 0.8849416718633378 1105 48-49 0.8656622936274303 1105 50-51 0.8690303827400854 1105 52-53 0.8893319803544486 1105 54-55 0.677115504963318 1105 56-57 0.7131706724831801 1105 58-59 0.6946307160274969 1105 60-61 0.7667926545608346 1105 62-63 0.6649969633271269 1105 64-65 0.6914186227626651 1105 66-67 0.7576513290856006 1105 68-69 0.6454786469239728 1105 70-71 0.5364040240874672 1105 72-73 0.5973626942809744 1105 74-75 0.6386927393260748 1105 76-77 0.5391234781422334 1105 78-79 0.5772615172560194 1105 80-81 0.6856917001709348 1105 82-83 0.8318064125797022 1105 84-85 0.8847158462984979 1105 86-87 0.8270601461115632 1105 88-89 0.9776216221845466 1105 90-91 1.1982769785404983 1105 92-93 1.265706118371181 1105 94-95 1.239524257927986 1105 96-97 1.514704791464851 1105 98-99 1.5848375478984664 1105 100-101 1.7645154746709402 1106 1 0.45710390374519605 1106 2 0.2648302481467937 1106 3 0.310331087579204 1106 4 0.3182747351335138 1106 5 0.27974494317092535 1106 6 0.34970854198490997 1106 7 0.32736488364766103 1106 8 0.40904342344482814 1106 9 0.5763108038738523 1106 10-11 0.5286884102659499 1106 12-13 0.4508294213304893 1106 14-15 0.6858644418333668 1106 16-17 0.6002430290061653 1106 18-19 0.6589356565238802 1106 20-21 0.5482447175863072 1106 22-23 0.6815875771179662 1106 24-25 0.7275850726316762 1106 26-27 0.7063581865257689 1106 28-29 0.7776027161406063 1106 30-31 0.7901565737979084 1106 32-33 0.9059668711391922 1106 34-35 0.8693951109976936 1106 36-37 0.8702107858847299 1106 38-39 0.9905122964665409 1106 40-41 0.7457913387933175 1106 42-43 0.7737419225557716 1106 44-45 0.866288961079654 1106 46-47 0.7131652261991235 1106 48-49 0.685381586804759 1106 50-51 0.526560836782167 1106 52-53 0.5460739997102237 1106 54-55 0.9420837268324433 1106 56-57 1.0528656505755265 1106 58-59 0.8091704146167373 1106 60-61 0.9524780510652384 1106 62-63 0.9744244754846676 1106 64-65 0.9632299762221486 1106 66-67 1.1282035944811426 1106 68-69 1.2275540959104418 1106 70-71 1.2040818130999362 1106 72-73 1.21515844756712 1106 74-75 1.2467383842776876 1106 76-77 1.3050486550886404 1106 78-79 1.130267198800631 1106 80-81 1.1002759362920749 1106 82-83 1.4151758574253854 1106 84-85 1.3908066547148863 1106 86-87 1.2798082322014075 1106 88-89 1.6783474635827886 1106 90-91 1.8714497360155775 1106 92-93 2.0380952560282672 1106 94-95 1.8053374891861083 1106 96-97 2.0270428915178087 1106 98-99 1.8628179513844323 1106 100-101 1.7200260914555763 1107 1 0.15529606960698317 1107 2 7.917053741337554E-4 1107 3 0.08846011910070928 1107 4 0.0808205446344914 1107 5 0.13791644480922116 1107 6 0.054296342101359585 1107 7 0.0830138658490327 1107 8 0.0669003950514977 1107 9 0.08635521482931097 1107 10-11 0.058010772152449874 1107 12-13 0.08484490237305664 1107 14-15 0.09539881180288745 1107 16-17 0.18389916947721474 1107 18-19 0.16049517747923403 1107 20-21 0.08108456414588261 1107 22-23 0.12815029474877093 1107 24-25 0.20516067971460217 1107 26-27 0.11669486495163284 1107 28-29 0.08274708028761069 1107 30-31 0.13092702966666536 1107 32-33 0.17920927047246238 1107 34-35 0.09168792646960355 1107 36-37 0.1789676787161838 1107 38-39 0.16692511285177858 1107 40-41 0.09163566069853601 1107 42-43 0.1984051299629357 1107 44-45 0.11513644541023638 1107 46-47 0.2756830647898454 1107 48-49 0.4664546993391525 1107 50-51 0.47008585647596846 1107 52-53 0.5720409940361222 1107 54-55 0.41883019621857187 1107 56-57 0.5812081743507989 1107 58-59 0.5842360606306016 1107 60-61 0.8888439448214491 1107 62-63 0.7675267919327098 1107 64-65 0.7046360567555361 1107 66-67 0.5441398778892577 1107 68-69 0.5773808475173432 1107 70-71 0.5617860863700201 1107 72-73 0.6934316149016659 1107 74-75 0.7014155200564751 1107 76-77 0.7703127803983207 1107 78-79 0.989539270132159 1107 80-81 1.080496506898644 1107 82-83 1.0490355005394036 1107 84-85 0.9309971423220631 1107 86-87 0.942567819373604 1107 88-89 1.099029382958065 1107 90-91 1.196902408093763 1107 92-93 1.3326571973236092 1107 94-95 1.3339651449954957 1107 96-97 1.5577449613651702 1107 98-99 1.3320627938910832 1107 100-101 1.2209497023288378 1108 1 0.010882800218364963 1108 2 -0.12923787507902063 1108 3 -0.006214883093303314 1108 4 0.05576053193502872 1108 5 -0.008400453218236237 1108 6 0.01111843090467346 1108 7 0.05417963512149271 1108 8 0.1578917790346921 1108 9 0.09643373467093141 1108 10-11 0.1501134880820132 1108 12-13 0.03855278378279792 1108 14-15 0.15989775093323289 1108 16-17 0.12674526852268997 1108 18-19 0.09837416090282147 1108 20-21 0.040681046498278306 1108 22-23 0.096788174248438 1108 24-25 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-1.3131912439960658 2316 68-69 -1.51718273972228 2316 70-71 -1.546265139466712 2316 72-73 -1.4205756376556202 2316 74-75 -1.5650737442125155 2316 76-77 -1.70431414507836 2316 78-79 -1.4677668083923727 2316 80-81 -1.6807501086700256 2316 82-83 -1.601883233039306 2316 84-85 -1.5533344432232 2316 86-87 -1.3656883327068527 2316 88-89 -1.7000621870173767 2316 90-91 -1.6777409744485823 2316 92-93 -1.8359351368321022 2316 94-95 -1.6253568525201985 2316 96-97 -1.4048349627319823 2316 98-99 -1.4917743355387287 2316 100-101 -1.5578189657078205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 51.0 5 85.0 6 286.0 7 618.0 8 1332.0 9 2529.0 10 4262.0 11 6314.0 12 9354.0 13 12268.0 14 15363.0 15 20939.0 16 27226.0 17 35011.0 18 29190.0 19 17700.0 20 15384.0 21 15422.0 22 15939.0 23 17762.0 24 20397.0 25 24041.0 26 28971.0 27 35101.0 28 42539.0 29 51511.0 30 61437.0 31 73977.0 32 89872.0 33 111226.0 34 143757.0 35 191239.0 36 251084.0 37 301517.0 38 164100.0 39 8492.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50637285465645 20.158198688755835 15.958273768670441 20.37715468791728 2 15.954912068133453 22.381258534149325 38.856249312799555 22.807580084917664 3 18.728080746805038 27.592788684181308 31.202400522135232 22.476730046878426 4 12.873095929823192 18.197613832578313 36.97887893027599 31.950411307322508 5 12.777746483755514 38.92307159013112 32.78491762931894 15.514264296794433 6 29.25832947427429 37.70005497466561 17.341609322403396 15.700006228656695 7 26.441009364624573 35.85118425399851 20.513835704729974 17.193970676646934 8 27.47880908406497 33.247558643998026 19.542547026230697 19.7310852457063 9 28.082499688568006 15.67970709144185 20.389750366948128 35.84804285304201 10-11 24.76929634708835 27.626999395550474 26.173070798588316 21.43063345877286 12-13 26.715804622058677 25.59599653035519 27.581482625913416 20.106716221672713 14-15 23.495810562689908 25.5339298384345 27.8384994935845 23.131760105291093 16-17 20.804090320693707 29.243166098867473 27.927785691460265 22.02495788897856 18-19 20.712339747929654 26.025694493340772 32.853258661871514 20.40870709685806 20-21 22.200948022858057 24.834916576100866 33.07799780475539 19.88613759628569 22-23 21.715448651634883 25.12335414962818 33.064409552025396 20.09678764671155 24-25 21.038932409515283 25.472430346686338 33.26427446755614 20.224362776242234 26-27 20.94873991248892 25.65514828326465 33.10060416063539 20.29550764361103 28-29 20.711532649353774 25.48590280643942 33.276813482868555 20.52575106133826 30-31 20.87809372855198 25.388359796385007 33.285185042737595 20.448361432325424 32-33 20.72594958888024 25.687659474440487 32.95639005383202 20.630000882847252 34-35 21.115787518100728 25.649317500651993 32.768011967942854 20.466883013304425 36-37 21.42922526533586 25.487949865808705 32.34186145392405 20.740963414931386 38-39 21.203205601786955 25.65580944911573 32.34847270277121 20.792512246326105 40-41 21.159225612158817 25.600025998892338 32.394836511652784 20.84591187729606 42-43 21.203020143315985 25.863335265156234 31.800359644039062 21.133284947488722 44-45 21.271322397576125 26.025267860334573 31.49488869077473 21.208521051314577 46-47 21.579708916467645 26.456358728782327 30.562768898047104 21.40116345670293 48-49 21.83134273751406 26.805408527782358 29.67394633556238 21.689302399141205 50-51 21.823618715630715 26.976905790857096 29.38641840170676 21.813057091805426 52-53 21.949888560000215 27.17169884389463 29.108804978646308 21.769607617458846 54-55 21.645130792756493 27.177286195528744 29.129042190958426 22.048540820756337 56-57 21.806953470750436 27.445497781780976 28.71091983376492 22.03662891370367 58-59 22.04328680759794 26.95364758515726 28.956312388628003 22.046753218616793 60-61 22.002529396790322 27.005020879710123 29.389424196632163 21.603025526867395 62-63 22.016508603647274 26.953924313901783 29.455562716986854 21.574004365464088 64-65 21.82759619889455 27.09722933101519 29.348644996357592 21.726529473732672 66-67 21.816229027752126 27.41892066820884 28.891832298927213 21.87301800511182 68-69 21.82482898321517 27.219968477666264 29.12165346014483 21.83354907897374 70-71 21.884220261251173 27.28795176318138 28.935645876973364 21.892182098594084 72-73 21.90364668102685 27.453719826962875 28.503083720005694 22.139549772004578 74-75 21.732726317006705 27.814841424612542 28.376045368611848 22.07638688976891 76-77 21.678521473381185 27.665097309615593 28.551140864351492 22.105240352651727 78-79 21.559477745810007 27.667328890583565 28.39925061095031 22.373942752656117 80-81 21.492352828544817 28.06572724433981 28.249049198477632 22.19287072863774 82-83 21.596719012648993 27.81900948044805 28.18406915335133 22.400202353551627 84-85 21.55951140657071 27.654525674770618 27.927594403431627 22.858368515227042 86-87 21.540056855188258 27.61249102582261 27.978770163294147 22.868681955694985 88-89 21.548105030485576 27.559696969532837 28.048977323628066 22.843220676353518 90-91 21.53380858860882 27.65752264250888 28.03701011641624 22.77165865246606 92-93 21.37090233246376 27.54743990647289 27.939140214573992 23.142517546489362 94-95 21.352278183497702 27.449044890433953 27.980944658971847 23.217732267096498 96-97 21.467611341514473 27.36834465430198 27.84056472504968 23.323479279133867 98-99 21.160922814938708 27.372439694431566 27.81259884826872 23.654038642361005 100-101 21.098446072935452 27.63967199085734 27.82149608133067 23.440385854876535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3566.0 1 2454.5 2 1441.5 3 1548.0 4 1318.0 5 1345.0 6 2384.5 7 4882.0 8 7471.5 9 8195.0 10 6774.5 11 5148.5 12 4892.5 13 5103.5 14 5312.0 15 5515.0 16 5763.0 17 5975.0 18 6204.0 19 6572.0 20 7108.0 21 7750.0 22 8583.0 23 9748.0 24 10982.0 25 12414.0 26 14554.5 27 16673.0 28 18197.5 29 20138.5 30 22849.0 31 26076.0 32 29930.5 33 33389.0 34 36280.0 35 38499.5 36 42210.0 37 46771.5 38 48885.5 39 49481.5 40 50088.0 41 51543.0 42 52740.0 43 55069.5 44 58021.5 45 59351.0 46 59045.0 47 59090.5 48 62328.5 49 62544.0 50 58974.0 51 55749.5 52 53433.0 53 54179.5 54 55268.0 55 53745.0 56 51029.0 57 47519.5 58 44462.0 59 41572.0 60 36796.5 61 31409.0 62 26523.0 63 20797.5 64 15419.5 65 11645.5 66 8229.0 67 6261.0 68 4368.0 69 2480.5 70 1733.5 71 1257.5 72 891.0 73 676.0 74 501.0 75 398.5 76 289.0 77 134.5 78 64.0 79 36.5 80 17.5 81 9.5 82 5.0 83 3.0 84 1.5 85 1.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015002897671571947 2 0.00276226635830386 3 0.0024372938455622293 4 1.0832417091387686E-4 5 0.0011915658800526457 6 2.708104272846922E-4 7 0.0 8 0.0 9 0.0 10-11 1.0832417091387686E-4 12-13 6.22863982754792E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0025185369737476372 22-23 0.0 24-25 0.003195563041959368 26-27 0.001597781520979684 28-29 0.003033076785588552 30-31 0.015002897671571947 32-33 5.957829400263228E-4 34-35 0.0024102128028337605 36-37 0.0035205355547009987 38-39 0.002274807589191414 40-41 0.004197561622912729 42-43 0.0021664834182775375 44-45 6.499450254832612E-4 46-47 8.124312818540765E-5 48-49 1.895672990992845E-4 50-51 1.624862563708153E-4 52-53 0.001354052136423461 54-55 0.0026810232301184523 56-57 4.874587691124459E-4 58-59 0.0010832417091387687 60-61 0.0010832417091387687 62-63 0.0 64-65 2.708104272846922E-4 66-67 5.416208545693843E-5 68-69 0.0 70-71 1.354052136423461E-4 72-73 5.416208545693843E-5 74-75 6.22863982754792E-4 76-77 6.770260682117305E-4 78-79 3.249725127416306E-4 80-81 0.0056599379302500665 82-83 0.0019769161191782528 84-85 0.003872589110171098 86-87 0.0021935644610060067 88-89 0.0014623763073373377 90-91 2.1664834182775371E-4 92-93 2.7081042728469214E-5 94-95 1.895672990992845E-4 96-97 0.0016790246491650916 98-99 0.002491455931019168 100-101 0.0010832417091387687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1846310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.972028455540695 #Duplication Level Percentage of deduplicated Percentage of total 1 67.83484693752577 27.793312790015527 2 15.138034379256066 12.404719506956656 3 6.2507426487101965 7.6831681701364785 4 3.223884568437093 5.283563611015324 5 1.9283513143338622 3.950423246158315 6 1.1957133294774906 2.9394480336012627 7 0.830032884732081 2.3805691680594148 8 0.5855893078298505 1.919422542693201 9 0.462199319936881 1.70435193197269 >10 2.269265923845869 16.756377123008573 >50 0.17604678820967506 4.972492802424467 >100 0.0991929090420143 7.1540017253224715 >500 0.0038454558963406677 1.0001554372029593 >1k 0.0018564269844403224 1.42914274407213 >5k 0.0 0.0 >10k+ 3.978057823800691E-4 2.6288511673605077 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20667 1.1193678201385466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16325 0.8841960450845199 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11395 0.6171769637818135 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3669 0.1987206915415071 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3637 0.1969875048068851 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3152 0.17071889336026994 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3056 0.16551933315640385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.7081042728469218E-5 0.0 14-15 0.0 0.0 0.0 8.124312818540765E-5 0.0 16-17 0.0 0.0 0.0 1.354052136423461E-4 0.0 18-19 0.0 0.0 0.0 1.8956729909928453E-4 0.0 20-21 0.0 0.0 0.0 4.0621564092703825E-4 0.0 22-23 0.0 0.0 0.0 0.001001998580953361 0.0 24-25 0.0 0.0 0.0 0.0016519436064366222 0.0 26-27 0.0 0.0 0.0 0.0027351853155753907 0.0 28-29 0.0 0.0 0.0 0.005741181058435474 0.0 30-31 0.0 0.0 0.0 0.012511441740552777 0.0 32-33 0.0 0.0 0.0 0.022071049823702414 0.0 34-35 0.0 0.0 0.0 0.03301179108600398 0.0 36-37 0.0 0.0 0.0 0.04517117927108666 0.0 38-39 0.0 0.0 0.0 0.061771858463638286 0.0 40-41 0.0 0.0 0.0 0.0875259300984125 0.0 42-43 0.0 0.0 0.0 0.11845248089432435 0.0 44-45 0.0 0.0 0.0 0.1502727061002757 0.0 46-47 0.0 0.0 0.0 0.18726541046736464 0.0 48-49 0.0 0.0 0.0 0.22623503095363184 0.0 50-51 0.0 0.0 0.0 0.27706614815496855 0.0 52-53 0.0 0.0 0.0 0.35042869290639167 0.0 54-55 0.0 0.0 0.0 0.43077814668175984 0.0 56-57 0.0 0.0 0.0 0.5115608971407835 0.0 58-59 0.0 0.0 0.0 0.6178539898500252 0.0 60-61 0.0 0.0 0.0 0.7428330020419106 0.0 62-63 0.0 0.0 0.0 0.8510217677421452 0.0 64-65 0.0 0.0 0.0 0.9596979922114921 0.0 66-67 0.0 0.0 0.0 1.1108102106363504 0.0 68-69 0.0 0.0 0.0 1.2722673873834838 0.0 70-71 0.0 0.0 0.0 1.4241649560474676 0.0 72-73 0.0 0.0 0.0 1.6078827499174029 0.0 74-75 0.0 0.0 0.0 1.8148902405338214 0.0 76-77 0.0 0.0 0.0 2.030942799421549 0.0 78-79 0.0 0.0 0.0 2.264923008595523 0.0 80-81 0.0 0.0 0.0 2.5367083534184403 0.0 82-83 0.0 0.0 0.0 2.8379578727299317 0.0 84-85 0.0 0.0 0.0 3.1373117190504303 0.0 86-87 0.0 0.0 0.0 3.463584121843027 0.0 88-89 0.0 0.0 0.0 3.8287990640791634 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9220 0.0 49.617687 1 GTATCAA 16510 0.0 41.865578 1 TATCAAC 21985 0.0 31.434538 2 ATCAACG 22225 0.0 31.073719 3 TCAACGC 22400 0.0 30.851322 4 CAACGCA 22850 0.0 30.222963 5 AACGCAG 23545 0.0 29.351015 6 ACGCAGA 26720 0.0 25.72118 7 CGCAGAG 26860 0.0 25.42797 8 GCAGAGT 27725 0.0 24.035046 9 GTACATG 14605 0.0 23.191515 1 GAGTACT 16145 0.0 22.82877 12-13 GTGGTAT 4465 0.0 22.555662 1 AGTACTT 16475 0.0 22.457989 12-13 CATATAG 405 0.0 22.282768 3 TACATGG 15135 0.0 22.281607 2 TGGTATC 4660 0.0 21.404444 2 AGAGTAC 25300 0.0 21.232489 10-11 GTACTTT 17275 0.0 21.131567 14-15 ACATGGG 15000 0.0 21.120546 3 >>END_MODULE