FastQCFastQC Report
Thu 26 May 2016
SRR937098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798894
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204321.135809002642735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143870.7997691915143416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96790.5380528257918477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.17627497784749963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31100.17288400539442567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24140.13419356560197543No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.12413182766744454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82500.051.4948431
GTATCAA156800.044.7928351
ATCAACG201050.034.6978153
TATCAAC202500.034.472832
TCAACGC203550.034.226874
CAACGCA207050.033.5583535
AACGCAG211500.032.8720056
ACGCAGA240750.028.6026617
CGCAGAG241000.028.3576568
GCAGAGT249250.026.580079
GTACATG151400.025.455121
GAGTACT134200.024.82250812-13
TACATGG154400.024.6835292
ACATGGG156300.023.6233883
AGTACTT136900.023.15274812-13
GTACTTT145050.022.84948714-15
TACACCG1703.1358686E-622.3650345
CATGGGG47400.022.1573734
AGAGTAC228800.021.87201310-11
CATGGGA109600.021.4634234
Produced by FastQC (version 0.11.5)