FastQCFastQC Report
Thu 26 May 2016
SRR937098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798894
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201331.119187678651438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159050.8841543748547719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109380.6080402736348001No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.1931186606881784No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.18494697297339366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29150.16204401148705816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.16115457608953054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89150.049.9345551
GTATCAA158750.041.8385281
TATCAAC213200.031.0126152
ATCAACG214750.030.7649483
TCAACGC217450.030.4022564
CAACGCA221550.029.8396325
AACGCAG228150.029.017256
ACGCAGA260200.025.2977527
CGCAGAG259250.025.0790278
GCAGAGT267200.023.6752059
GTACATG146200.023.267441
AGTACTT155000.022.9822112-13
GAGTACT151950.022.9277612-13
TACATGG150900.022.2229562
GTGGTAT45000.021.4321881
ACATGGG151450.021.356993
GTACTTT163050.021.10619714-15
AGAGTAC246450.021.0821310-11
TGGTATC44900.020.9461982
TACTTTT172550.020.57731214-15
Produced by FastQC (version 0.11.5)