##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1798894 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775031769520606 31.0 31.0 34.0 30.0 34.0 2 31.82362329297891 31.0 31.0 34.0 30.0 34.0 3 31.93506232162651 31.0 31.0 34.0 30.0 34.0 4 35.73976176472877 37.0 35.0 37.0 35.0 37.0 5 35.629947067475904 37.0 35.0 37.0 33.0 37.0 6 35.67638893675781 37.0 35.0 37.0 33.0 37.0 7 35.60761890361522 37.0 35.0 37.0 33.0 37.0 8 35.59421511217448 37.0 35.0 37.0 33.0 37.0 9 37.170613165645115 39.0 37.0 39.0 33.0 39.0 10-11 37.06498520757754 39.0 37.0 39.0 33.0 39.0 12-13 36.95537202303193 39.0 37.0 39.0 33.0 39.0 14-15 37.9713515637942 40.0 37.0 41.0 33.0 41.0 16-17 37.904477417791156 40.0 37.0 41.0 33.0 41.0 18-19 38.00939271574646 40.0 37.0 41.0 33.0 41.0 20-21 38.13513886866041 40.0 37.0 41.0 33.0 41.0 22-23 38.04864850291345 40.0 37.0 41.0 33.0 41.0 24-25 37.92807219324763 40.0 37.0 41.0 33.0 41.0 26-27 37.69639206089964 40.0 37.0 41.0 32.5 41.0 28-29 37.489661703246554 39.0 36.0 41.0 32.0 41.0 30-31 37.282591136553904 39.0 36.0 41.0 32.0 41.0 32-33 37.06935956204201 39.0 35.0 41.0 31.0 41.0 34-35 36.87673648363939 39.0 35.0 41.0 31.0 41.0 36-37 36.60478910930827 39.0 35.0 41.0 30.5 41.0 38-39 36.387866099948084 39.0 35.0 40.5 30.0 41.0 40-41 36.05189633185724 38.5 35.0 40.0 30.0 41.0 42-43 35.67670802170667 38.0 35.0 40.0 28.5 41.0 44-45 35.24762576338573 38.0 35.0 40.0 26.5 41.0 46-47 34.63693469431773 38.0 34.0 40.0 24.5 41.0 48-49 34.0634967930295 38.0 33.0 40.0 22.0 41.0 50-51 33.71507465142471 38.0 33.0 40.0 19.5 41.0 52-53 33.43147428364317 38.0 33.0 40.0 18.5 41.0 54-55 33.10853252053762 37.0 33.0 40.0 15.5 41.0 56-57 32.818062654052994 37.0 32.0 40.0 13.5 41.0 58-59 32.423918807889734 36.0 31.5 39.5 10.0 41.0 60-61 32.05708868893887 36.0 31.0 39.0 8.0 40.5 62-63 31.685975938548907 35.0 31.0 39.0 7.0 40.0 64-65 31.239357349571456 35.0 30.5 38.5 4.0 40.0 66-67 30.78290716406859 35.0 30.0 38.0 2.0 40.0 68-69 30.33622742640756 34.5 29.0 37.5 2.0 40.0 70-71 29.933460504065277 34.0 29.0 37.0 2.0 39.0 72-73 29.544184093114993 34.0 29.0 36.5 2.0 39.0 74-75 29.06040489322884 34.0 28.0 36.0 2.0 39.0 76-77 27.672807569539952 32.0 26.0 34.5 2.0 36.5 78-79 28.385669194516186 33.5 27.0 35.0 2.0 37.0 80-81 28.136205079343195 34.0 27.0 35.0 2.0 37.0 82-83 27.891678720369292 33.0 27.0 35.0 2.0 36.0 84-85 27.525768333209186 33.0 26.0 35.0 2.0 36.0 86-87 27.111272815407688 33.0 26.0 35.0 2.0 35.5 88-89 26.68871428777905 33.0 25.0 35.0 2.0 35.0 90-91 26.171969276677782 32.0 24.0 35.0 2.0 35.0 92-93 25.65576876680894 32.0 22.5 34.0 2.0 35.0 94-95 25.06956413218344 32.0 19.5 34.0 2.0 35.0 96-97 24.31000436935139 31.0 12.0 34.0 2.0 35.0 98-99 23.39934426375317 31.0 2.0 34.0 2.0 35.0 100-101 20.315652840022814 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36818029498583016 1101 2 0.2711343056906834 1101 3 0.28383656741579344 1101 4 0.24889693366891663 1101 5 0.33318336267789306 1101 6 0.24419287350693963 1101 7 0.2465027049849411 1101 8 0.3639935732319941 1101 9 0.3897722981810361 1101 10-11 0.35731670295390217 1101 12-13 0.3791595945814308 1101 14-15 0.47671356197666626 1101 16-17 0.4880207166299684 1101 18-19 0.39519629674784795 1101 20-21 0.32869875702603935 1101 22-23 0.45660489245835123 1101 24-25 0.3623848283688247 1101 26-27 0.4358479780040341 1101 28-29 0.4925626900128748 1101 30-31 0.40806824858384516 1101 32-33 0.4818159738412575 1101 34-35 0.6124560668046044 1101 36-37 0.5821037924130792 1101 38-39 0.5490419244252607 1101 40-41 0.6970098281230221 1101 42-43 0.9479937745378066 1101 44-45 0.7185627630806977 1101 46-47 0.7540661570717333 1101 48-49 0.5540247924101109 1101 50-51 0.3156146579884478 1101 52-53 0.43569573040411314 1101 54-55 0.44922774057969406 1101 56-57 0.4197205419679051 1101 58-59 0.509169742279461 1101 60-61 0.5917690565370606 1101 62-63 0.6327390355235067 1101 64-65 0.7474966962997165 1101 66-67 0.6233012538282772 1101 68-69 0.4709154191917797 1101 70-71 0.7632823447635353 1101 72-73 0.6447498057084715 1101 74-75 0.48960407895122415 1101 76-77 0.40892826399427307 1101 78-79 0.5514353193281352 1101 80-81 1.0563672535706345 1101 82-83 0.844827869804849 1101 84-85 0.6669417921174379 1101 86-87 0.38458187341540295 1101 88-89 0.23883778667129008 1101 90-91 0.19381605559779302 1101 92-93 0.5036821718654352 1101 94-95 0.5797710290768805 1101 96-97 0.6252158683466824 1101 98-99 0.7927598818412172 1101 100-101 0.7917161796784207 1102 1 0.3394261557782521 1102 2 0.35744939741167414 1102 3 0.24744912279017584 1102 4 0.27774014536849023 1102 5 0.24459943774736104 1102 6 0.33510008127666424 1102 7 0.40006013010930275 1102 8 0.3391145313087378 1102 9 0.4220129892178548 1102 10-11 0.3769022939247364 1102 12-13 0.4140162352168275 1102 14-15 0.4082026113867485 1102 16-17 0.4262427290764421 1102 18-19 0.4770527331971408 1102 20-21 0.4533669545953458 1102 22-23 0.5810897664104147 1102 24-25 0.5169625922053953 1102 26-27 0.5371183707219984 1102 28-29 0.5456056549135795 1102 30-31 0.7696136415237405 1102 32-33 0.6625641827739557 1102 34-35 0.6083270685298245 1102 36-37 0.7625932176439747 1102 38-39 0.7851713251538186 1102 40-41 0.8408286114697248 1102 42-43 0.7777475379245757 1102 44-45 0.8433447426206513 1102 46-47 1.0536176874318386 1102 48-49 1.284168284939561 1102 50-51 1.1627714403704488 1102 52-53 0.8862409665054045 1102 54-55 0.9774342763107811 1102 56-57 0.9818299620497513 1102 58-59 1.0177033900791415 1102 60-61 0.9711816219727893 1102 62-63 0.9799076547815133 1102 64-65 1.0342086192890072 1102 66-67 1.0367498684699008 1102 68-69 0.9392792965972561 1102 70-71 1.0279292951403889 1102 72-73 1.046017387168373 1102 74-75 1.1405684884242788 1102 76-77 1.0447984371381764 1102 78-79 1.0673769280187706 1102 80-81 0.9916276796389809 1102 82-83 1.0079499346294973 1102 84-85 0.9212749765034154 1102 86-87 1.059332633194881 1102 88-89 0.9708762150892625 1102 90-91 0.937021984688986 1102 92-93 1.0171088882864368 1102 94-95 1.171828337312089 1102 96-97 1.166877676962148 1102 98-99 1.3843259597219415 1102 100-101 1.649119405818979 1103 1 0.2704107021886166 1103 2 0.23631598302705825 1103 3 0.2686241964023175 1103 4 0.3056130025396868 1103 5 0.26634130357064123 1103 6 0.3339441085620436 1103 7 0.42332751716887174 1103 8 0.3415491872516938 1103 9 0.3978988145948392 1103 10-11 0.2950991578634259 1103 12-13 0.4665371852739355 1103 14-15 0.6232948709889143 1103 16-17 0.5334429739306827 1103 18-19 0.4566655007584828 1103 20-21 0.46857568999689647 1103 22-23 0.5855899548688299 1103 24-25 0.45374967387731857 1103 26-27 0.5217084240854746 1103 28-29 0.5147059856368301 1103 30-31 0.5121285835834044 1103 32-33 0.49019388608656556 1103 34-35 0.49674821214647125 1103 36-37 0.6409026465157979 1103 38-39 0.424181507575895 1103 40-41 0.5418605983881548 1103 42-43 0.5033870920858234 1103 44-45 0.7328850174582016 1103 46-47 0.6132698753914738 1103 48-49 0.38287684888647533 1103 50-51 0.5334447611776696 1103 52-53 0.5695947043583374 1103 54-55 0.5376518395010237 1103 56-57 0.5595623964506373 1103 58-59 0.7473307525399164 1103 60-61 0.6429763411697635 1103 62-63 0.6770080875273692 1103 64-65 0.6966036564564 1103 66-67 0.5598062671288702 1103 68-69 0.7083500124274771 1103 70-71 0.6289434281041757 1103 72-73 0.7090895993300315 1103 74-75 0.686369550026086 1103 76-77 0.7368273548767874 1103 78-79 0.7546566938202268 1103 80-81 0.9721613428453821 1103 82-83 0.8838366101948019 1103 84-85 0.7238405614471475 1103 86-87 0.7873691516170425 1103 88-89 0.8944050350562165 1103 90-91 1.0101275525722713 1103 92-93 1.0567910028748173 1103 94-95 1.2020970251597838 1103 96-97 1.5409924183773747 1103 98-99 1.6492187084657886 1103 100-101 1.8072062553894916 1104 1 0.22973541151332455 1104 2 0.18501193172300745 1104 3 0.24700147477959078 1104 4 0.1567454536721371 1104 5 0.042761129990466884 1104 6 0.1571073817253179 1104 7 0.14275188659323845 1104 8 0.25593830164081055 1104 9 0.28810220479823556 1104 10-11 0.2349843727486487 1104 12-13 0.17211941585616586 1104 14-15 0.24395894165298415 1104 16-17 0.4201002355879453 1104 18-19 0.2915850356780183 1104 20-21 0.4238188452400564 1104 22-23 0.20131722059608848 1104 24-25 0.26373273386037965 1104 26-27 0.36471681709387127 1104 28-29 0.2870338079646544 1104 30-31 0.547106968561792 1104 32-33 0.4087462296389148 1104 34-35 0.3676963580946122 1104 36-37 0.4118582174713765 1104 38-39 0.49535542874981786 1104 40-41 0.4958764274039851 1104 42-43 0.3987736874724206 1104 44-45 0.499194733767915 1104 46-47 0.51187562399722 1104 48-49 0.7444384104480406 1104 50-51 0.7705436732765776 1104 52-53 0.6967850815487182 1104 54-55 0.591547493396682 1104 56-57 0.6800721919604342 1104 58-59 0.6283821335913018 1104 60-61 0.624111322304735 1104 62-63 0.5791151396344176 1104 64-65 0.609025968878715 1104 66-67 0.4462311535537573 1104 68-69 0.6984989525764185 1104 70-71 0.7943071434678863 1104 72-73 0.5846318998723312 1104 74-75 0.4670994557559922 1104 76-77 0.6461108141602487 1104 78-79 0.9306068197703539 1104 80-81 1.0965244422084837 1104 82-83 0.8851593615175553 1104 84-85 0.655600893207481 1104 86-87 0.8560056452535356 1104 88-89 0.8126067532579384 1104 90-91 0.6814100988548191 1104 92-93 0.7979313190151345 1104 94-95 1.096268944331701 1104 96-97 1.2294597318446883 1104 98-99 1.547389681636762 1104 100-101 1.5752627734460098 1105 1 0.27488706504648164 1105 2 0.1676594201508408 1105 3 0.13067131220665473 1105 4 0.1750069141301296 1105 5 0.2266487546295366 1105 6 0.22407053105840902 1105 7 0.27812709566786964 1105 8 0.24636975739018396 1105 9 0.14442546285559388 1105 10-11 0.1980682932025033 1105 12-13 0.1402956896682781 1105 14-15 0.3544631460878307 1105 16-17 0.4811195747864687 1105 18-19 0.3944109708738992 1105 20-21 0.3846608465444845 1105 22-23 0.04120292464364894 1105 24-25 0.34349491044336844 1105 26-27 0.2825091091636196 1105 28-29 0.1209463412991525 1105 30-31 0.14112582269625307 1105 32-33 0.224843992991012 1105 34-35 0.3655336442266943 1105 36-37 0.3787181814411653 1105 38-39 0.3933586909149227 1105 40-41 0.5615948247697773 1105 42-43 0.445674958362126 1105 44-45 0.6008402371475299 1105 46-47 0.5623506918133288 1105 48-49 0.6311748578781291 1105 50-51 0.5380167680618158 1105 52-53 0.6032070457741483 1105 54-55 0.32984586897828905 1105 56-57 0.29894743872585394 1105 58-59 0.5818777084741598 1105 60-61 0.6114958328106397 1105 62-63 0.6360047427667936 1105 64-65 0.5460288191763603 1105 66-67 0.43357770014885944 1105 68-69 0.5071600227981854 1105 70-71 0.44869517068828557 1105 72-73 0.46718152880924535 1105 74-75 0.32361594738809174 1105 76-77 0.5393527464195245 1105 78-79 0.6076112323239009 1105 80-81 0.8058228964491327 1105 82-83 0.8012129610971677 1105 84-85 0.6294260057609158 1105 86-87 0.559335025606039 1105 88-89 0.4683836041214917 1105 90-91 0.35447732611280003 1105 92-93 0.6403804064064182 1105 94-95 0.3954222398722784 1105 96-97 0.6891940882553449 1105 98-99 1.1550153203317635 1105 100-101 1.3253452561874504 1106 1 0.20607336181487312 1106 2 0.2406520394359184 1106 3 0.19476786137307656 1106 4 0.12124580998239765 1106 5 0.1343481085845255 1106 6 0.2552761151316645 1106 7 0.17603641558049787 1106 8 0.24848206263755657 1106 9 0.3277167590740575 1106 10-11 0.2809560171783332 1106 12-13 0.1517590169568379 1106 14-15 0.3627505768753636 1106 16-17 0.3978442450559143 1106 18-19 0.3543324295679824 1106 20-21 0.32345181413490565 1106 22-23 0.40639847825570996 1106 24-25 0.2019333985524483 1106 26-27 0.3350290247670529 1106 28-29 0.3424043180874392 1106 30-31 0.31530559419216786 1106 32-33 0.42011321029305293 1106 34-35 0.3628633088985751 1106 36-37 0.30400604313790325 1106 38-39 0.3191526451152811 1106 40-41 0.5517685274062956 1106 42-43 0.49536792890993553 1106 44-45 0.47270585085841077 1106 46-47 0.4231154786142213 1106 48-49 0.5969033627613243 1106 50-51 0.3234092381492246 1106 52-53 0.45161839351043653 1106 54-55 0.6623497065143624 1106 56-57 0.5003486109476682 1106 58-59 0.5415312436564861 1106 60-61 0.5235141084618249 1106 62-63 0.5639341151005084 1106 64-65 0.4425449899609326 1106 66-67 0.45489296150777747 1106 68-69 0.517516986710735 1106 70-71 0.5741058229126743 1106 72-73 0.4566631731927906 1106 74-75 0.6605404564517983 1106 76-77 0.3632414549511509 1106 78-79 0.5135109691557993 1106 80-81 0.9466032193600604 1106 82-83 0.8179197255216017 1106 84-85 0.7468615168240191 1106 86-87 0.8819088234570209 1106 88-89 0.6940179731423619 1106 90-91 0.8038465568575042 1106 92-93 1.0117655643205623 1106 94-95 1.074696144573867 1106 96-97 1.1555581975033142 1106 98-99 1.3467462735868843 1106 100-101 1.3861955075334436 1107 1 0.12047043934512658 1107 2 0.09784578760121576 1107 3 0.1218233344804851 1107 4 0.1257092220310625 1107 5 0.0882131033658311 1107 6 0.12696499523212168 1107 7 0.146468731539926 1107 8 0.12699092218939967 1107 9 0.1548459258766286 1107 10-11 0.10073365180437577 1107 12-13 0.13156001340160373 1107 14-15 0.13798327771360874 1107 16-17 0.17619210987538025 1107 18-19 0.2325372898863307 1107 20-21 0.20289815330121996 1107 22-23 -0.010235911319945501 1107 24-25 0.2101434164101761 1107 26-27 0.21097440377484844 1107 28-29 0.22300651687083217 1107 30-31 0.3513726952065497 1107 32-33 0.327476005475539 1107 34-35 0.3871241295385133 1107 36-37 0.4096843818801972 1107 38-39 0.4589928021654117 1107 40-41 0.4827181466045758 1107 42-43 0.31981614354511834 1107 44-45 0.38016190984598097 1107 46-47 0.45376206689172705 1107 48-49 0.5523884976320019 1107 50-51 0.5549960071240747 1107 52-53 0.5924561674919815 1107 54-55 0.5240333679188467 1107 56-57 0.5022645960580832 1107 58-59 0.6039695774247136 1107 60-61 0.45753913985554107 1107 62-63 0.5292002278243473 1107 64-65 0.5831526622594403 1107 66-67 0.47035920918382956 1107 68-69 0.5167492166432162 1107 70-71 0.2235980193313445 1107 72-73 0.3194145267920554 1107 74-75 0.36611172019164684 1107 76-77 0.2780745280292045 1107 78-79 0.33298384009092175 1107 80-81 0.5565691449447172 1107 82-83 0.6341512970014271 1107 84-85 0.4007921530156757 1107 86-87 0.25244744996146906 1107 88-89 0.176059594281341 1107 90-91 0.2327538006521479 1107 92-93 0.4469367722040545 1107 94-95 0.4924545413741548 1107 96-97 0.5265154918540738 1107 98-99 0.7806319189112578 1107 100-101 1.1176994602879375 1108 1 0.12779297133559098 1108 2 0.008575314109918963 1108 3 -0.056274652025944505 1108 4 0.07125498582872325 1108 5 -0.04981900670731676 1108 6 0.044511754423808725 1108 7 0.06465002907961548 1108 8 0.13926280261237167 1108 9 -3.1517185443874496E-4 1108 10-11 0.08901091059870936 1108 12-13 0.08486417418915693 1108 14-15 0.13999864651622573 1108 16-17 0.11492684953220333 1108 18-19 0.2198798463257745 1108 20-21 0.18272254532022458 1108 22-23 0.03723232121707554 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 27.0 5 99.0 6 220.0 7 516.0 8 1130.0 9 2235.0 10 3679.0 11 5757.0 12 8357.0 13 11344.0 14 14317.0 15 19242.0 16 25659.0 17 33737.0 18 28767.0 19 16610.0 20 14512.0 21 14253.0 22 14842.0 23 16501.0 24 18815.0 25 22331.0 26 26550.0 27 32331.0 28 39493.0 29 47664.0 30 57499.0 31 69351.0 32 85251.0 33 106582.0 34 140149.0 35 187804.0 36 251452.0 37 306141.0 38 167381.0 39 8285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78829993733559 20.022141054419066 15.82362553927813 20.365933468967214 2 16.005474846172834 22.257295532730108 38.91193547598028 22.82529414511678 3 18.78812744720323 27.52870486346809 31.143630224985642 22.539537464343038 4 12.860068464289725 18.18706382921951 36.961933276780066 31.9909344297107 5 12.798124999305102 38.93005771542106 32.75725389782002 15.514563387453817 6 29.273876059400944 37.669590314937956 17.31958636806838 15.736947257592721 7 26.525298322191304 35.84947195332243 20.490312380829554 17.134917343656713 8 27.517271742828363 33.13862088441107 19.55787229027646 19.786235082484108 9 28.09666833991794 15.744849749262462 20.38058961817073 35.777892292648865 10-11 24.871587764481955 27.606212483892882 26.09989804846756 21.422301703157608 12-13 26.77318226252111 25.571747934769828 27.509246666627753 20.145823136081304 14-15 23.55227712138681 25.504643408672216 27.775538747697198 23.16754072224378 16-17 20.84065612592192 29.26044774771144 27.864894650458545 22.034001475908095 18-19 20.76423004674739 26.00475757122598 32.779126503696595 20.451885878330035 20-21 22.215761349185943 24.822102856666366 33.049524352194794 19.9126114419529 22-23 21.765679356315605 25.16307242116545 32.93217943914428 20.13906878337467 24-25 21.15649321770069 25.446464309539696 33.17333778074272 20.2237046920169 26-27 21.025636749250186 25.63861161839797 33.00908668907068 20.32666494328116 28-29 20.739073933800793 25.557147721665892 33.16173149222719 20.542046852306132 30-31 20.947053213702826 25.40469828700756 33.141663168021175 20.506585331268436 32-33 20.740648502431785 25.75626096861219 32.85502725858213 20.648063270373893 34-35 21.165785113353884 25.6717830948011 32.67170276407199 20.490729027773032 36-37 21.4957243454472 25.483129088044766 32.21170084623517 20.809445720272866 38-39 21.24202155604466 25.688880018546183 32.27205180477809 20.797046620631072 40-41 21.187146221858626 25.676700810609386 32.2944212472449 20.841731720287086 42-43 21.25611384567147 25.885736268794616 31.700824889675594 21.157324995858318 44-45 21.25724501924037 26.059637918593392 31.37542235974432 21.307694702421916 46-47 21.653203088279806 26.491141222064684 30.47587493339552 21.379780756259986 48-49 21.92278699715599 26.824814272086666 29.576850552680906 21.675548178076433 50-51 21.86844455463901 26.96289855926477 29.26524532357963 21.903411562516588 52-53 22.028057455772302 27.214942703496607 28.980159207758092 21.776840632973 54-55 21.69419276617496 27.191148213446397 29.009763637531037 22.104895382847605 56-57 21.855874410919 27.489983364672234 28.64225428842629 22.01188793598248 58-59 22.126582306627967 26.977469832567763 28.889106926154483 22.006840934649784 60-61 22.097917414232693 27.006796122171767 29.27771988725291 21.61756657634262 62-63 22.02863094136227 26.95807921871951 29.434491100915956 21.578798739002263 64-65 21.8666783043004 27.15642758741846 29.250534534307487 21.726359573973657 66-67 21.796373798244964 27.401640854765425 28.899879063605983 21.90210628338363 68-69 21.866133371875833 27.193614915947702 29.041459130125414 21.898792582051055 70-71 21.88175523456412 27.338115498545868 28.887561487661607 21.892567779228404 72-73 21.915895072882115 27.499530205297507 28.452129309740933 22.132445412079452 74-75 21.740202421260932 27.871824885123115 28.32749648579991 22.06047620781605 76-77 21.65708489836784 27.719268596473622 28.478490428840537 22.145156076318 78-79 21.61125749814657 27.67415478281609 28.31887238216384 22.395715336873494 80-81 21.636500892493327 28.019343007060453 28.116180339797864 22.227975760648356 82-83 21.700997471922214 27.792539182426033 28.12117539634117 22.38528794931058 84-85 21.627099498466272 27.647449916114564 27.903859174754096 22.821591410665068 86-87 21.558969010958958 27.60118161492561 27.936554349505865 22.903295024609566 88-89 21.578459342208546 27.60549968091044 27.930120498002076 22.88592047887894 90-91 21.5936949307065 27.67181619572287 27.965639540378127 22.768849333192502 92-93 21.36097411289641 27.598327411156227 27.890396733982325 23.150301741965038 94-95 21.38090903405577 27.447436262690132 27.956417508043163 23.21523719521093 96-97 21.424038872849682 27.44366597077476 27.790657630356048 23.34163752601951 98-99 21.19775038926804 27.42395445188669 27.68319779597296 23.695097362872307 100-101 21.13543458580744 27.64971194568619 27.738461636813362 23.476391831693004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3085.0 1 2119.0 2 1313.5 3 1459.5 4 1251.0 5 1234.5 6 2125.5 7 4408.0 8 6966.5 9 7825.0 10 6431.0 11 4899.0 12 4699.0 13 4905.0 14 5185.0 15 5511.5 16 5681.0 17 5841.0 18 6147.0 19 6483.0 20 6983.0 21 7596.5 22 8251.5 23 9427.5 24 10700.5 25 12085.5 26 14163.0 27 15936.5 28 17469.0 29 19484.5 30 22258.5 31 25368.5 32 28940.5 33 32470.0 34 35536.0 35 37750.5 36 41187.5 37 45782.5 38 47760.5 39 48456.0 40 49000.5 41 49875.0 42 51016.0 43 53471.5 44 56256.5 45 57809.5 46 57638.0 47 57584.0 48 61039.0 49 61050.5 50 57340.5 51 54291.0 52 52080.5 53 53219.0 54 54220.5 55 52432.0 56 49957.5 57 46389.0 58 43588.5 59 40686.0 60 36013.5 61 30719.0 62 25810.0 63 20427.0 64 15105.0 65 11459.0 66 8051.5 67 6039.5 68 4273.0 69 2494.0 70 1759.0 71 1249.5 72 844.5 73 644.5 74 507.0 75 385.0 76 281.5 77 136.5 78 49.0 79 29.0 80 15.0 81 6.5 82 5.0 83 4.0 84 2.0 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02362562774682666 2 0.006781944906147889 3 0.008727584838239495 4 0.0 5 0.0037801004394922657 6 0.0 7 0.0 8 1.11179424690949E-4 9 5.55897123454745E-5 10-11 0.0 12-13 1.3897428086368625E-4 14-15 0.0 16-17 8.338456851821174E-5 18-19 0.004280407850601536 20-21 0.0017510759388824468 22-23 0.0 24-25 0.005225432960474603 26-27 0.00605927864565672 28-29 0.008699789982066759 30-31 0.03218644344802973 32-33 6.114868358002195E-4 34-35 0.004474971843810697 36-37 0.015592914312905597 38-39 0.007838149440711904 40-41 0.01031189164008552 42-43 0.006003688933311245 44-45 0.004002459288874164 46-47 0.0015843068018460233 48-49 0.0014453325209823368 50-51 0.003085229035173835 52-53 0.0027516907611009876 54-55 0.0014175376648095997 56-57 6.392816919729567E-4 58-59 9.450251098730664E-4 60-61 2.779485617273725E-5 62-63 5.55897123454745E-5 64-65 0.0073378420296026335 66-67 8.060508290093802E-4 68-69 7.78255972836643E-4 70-71 0.003196408459864784 72-73 0.013119172113531983 74-75 0.006698560337629677 76-77 0.004363792419119748 78-79 0.011090147612922163 80-81 5.55897123454745E-5 82-83 0.005364407241338289 84-85 6.670765481456939E-4 86-87 0.0 88-89 0.0014453325209823368 90-91 0.0013341530962913879 92-93 3.3353827407284697E-4 94-95 3.613331302455842E-4 96-97 1.9456399320916075E-4 98-99 2.779485617273725E-4 100-101 8.060508290093802E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1798894.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.31433676804788 #Duplication Level Percentage of deduplicated Percentage of total 1 68.06216394648307 28.11943162447088 2 15.103353441055578 12.479700607812498 3 6.231393913362047 7.723377200130102 4 3.231599207455725 5.340455118247299 5 1.8777558775862953 3.8789119347390746 6 1.1964026422650698 2.9657149003632846 7 0.7918682319410316 2.29008575672313 8 0.5659898377272218 1.870679581052417 9 0.4164009451222406 1.5482995989582304 >10 2.2434625891969455 16.665955328637466 >50 0.17568789601007773 5.02698351918946 >100 0.09759496898103327 7.066994629413455 >500 0.004038193285345462 1.0220329088943718 >1k 0.0018844901998278825 1.3889592954916656 >5k 0.0 0.0 >10k+ 4.038193285345462E-4 2.6124179958767013 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20133 1.119187678651438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15905 0.8841543748547719 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10938 0.6080402736348001 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3474 0.1931186606881784 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3327 0.18494697297339366 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2915 0.16204401148705816 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2899 0.16115457608953054 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.779485617273725E-5 0.0 12-13 0.0 0.0 0.0 8.338456851821174E-5 0.0 14-15 0.0 0.0 0.0 1.11179424690949E-4 0.0 16-17 0.0 0.0 0.0 3.3353827407284697E-4 0.0 18-19 0.0 0.0 0.0 4.7251255493653327E-4 0.0 20-21 0.0 0.0 0.0 7.226662604911684E-4 0.0 22-23 0.0 0.0 0.0 0.0013897428086368626 0.0 24-25 0.0 0.0 0.0 0.0021679987814735057 0.0 26-27 0.0 0.0 0.0 0.0034187673092466817 0.0 28-29 0.0 0.0 0.0 0.00555897123454745 0.0 30-31 0.0 0.0 0.0 0.013007992688841032 0.0 32-33 0.0 0.0 0.0 0.023681217459172135 0.0 34-35 0.0 0.0 0.0 0.03424326280481229 0.0 36-37 0.0 0.0 0.0 0.04452735958872507 0.0 38-39 0.0 0.0 0.0 0.05870273623682107 0.0 40-41 0.0 0.0 0.0 0.08116098002439276 0.0 42-43 0.0 0.0 0.0 0.11062352756749425 0.0 44-45 0.0 0.0 0.0 0.13941899856245005 0.0 46-47 0.0 0.0 0.0 0.1768586698271271 0.0 48-49 0.0 0.0 0.0 0.21279741885847636 0.0 50-51 0.0 0.0 0.0 0.2587979058243565 0.0 52-53 0.0 0.0 0.0 0.33256545410680116 0.0 54-55 0.0 0.0 0.0 0.4205639687496873 2.779485617273725E-5 56-57 0.0 0.0 0.0 0.5050881263709813 5.55897123454745E-5 58-59 0.0 0.0 0.0 0.6080680684909727 5.55897123454745E-5 60-61 0.0 0.0 0.0 0.7364803040090189 5.55897123454745E-5 62-63 0.0 0.0 0.0 0.8465757293092311 5.55897123454745E-5 64-65 0.0 0.0 0.0 0.9565043854724069 5.55897123454745E-5 66-67 0.0 0.0 0.0 1.1097930172650528 5.55897123454745E-5 68-69 0.0 0.0 0.0 1.274588719513212 5.55897123454745E-5 70-71 0.0 0.0 0.0 1.4178989979398453 5.55897123454745E-5 72-73 0.0 0.0 0.0 1.6041801240095301 5.55897123454745E-5 74-75 0.0 0.0 0.0 1.8026076022267015 5.55897123454745E-5 76-77 0.0 0.0 0.0 2.0193518906617065 5.55897123454745E-5 78-79 0.0 0.0 0.0 2.2488262232238254 5.55897123454745E-5 80-81 0.0 0.0 0.0 2.5152677144956845 5.55897123454745E-5 82-83 0.0 0.0 0.0 2.815174212599519 5.55897123454745E-5 84-85 0.0 0.0 0.0 3.119555682547165 5.55897123454745E-5 86-87 0.0 0.0 0.0 3.4549840068397586 5.55897123454745E-5 88-89 0.0 0.0 0.0 3.8392756882840233 5.55897123454745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8915 0.0 49.934555 1 GTATCAA 15875 0.0 41.838528 1 TATCAAC 21320 0.0 31.012615 2 ATCAACG 21475 0.0 30.764948 3 TCAACGC 21745 0.0 30.402256 4 CAACGCA 22155 0.0 29.839632 5 AACGCAG 22815 0.0 29.01725 6 ACGCAGA 26020 0.0 25.297752 7 CGCAGAG 25925 0.0 25.079027 8 GCAGAGT 26720 0.0 23.675205 9 GTACATG 14620 0.0 23.26744 1 AGTACTT 15500 0.0 22.98221 12-13 GAGTACT 15195 0.0 22.92776 12-13 TACATGG 15090 0.0 22.222956 2 GTGGTAT 4500 0.0 21.432188 1 ACATGGG 15145 0.0 21.35699 3 GTACTTT 16305 0.0 21.106197 14-15 AGAGTAC 24645 0.0 21.08213 10-11 TGGTATC 4490 0.0 20.946198 2 TACTTTT 17255 0.0 20.577312 14-15 >>END_MODULE