FastQCFastQC Report
Thu 26 May 2016
SRR937097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818941
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201601.1083372137963792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141880.7800143050269359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93840.5159045840409336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30450.16740510000049477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.16240218896599726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.12969084758659022No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.11974000256193026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82300.053.2956771
GTATCAA156700.045.2773441
TATCAAC200400.035.2152522
ATCAACG202700.034.8156743
TCAACGC203700.034.6447564
CAACGCA207650.033.985735
AACGCAG213800.033.1396646
ACGCAGA241200.029.099227
CGCAGAG240750.028.9767658
GCAGAGT249800.027.3905329
GTACATG150850.025.737531
GAGTACT139200.025.15560512-13
TACATGG154700.024.9133782
ACATGGG156400.023.6702463
GTACTTT151100.022.82511314-15
AGTACTT142600.022.77327512-13
CAGAGTA245850.022.0715210-11
AGAGTAC230300.021.83902710-11
CATGGGA107600.021.5530954
GTGGTAT36150.020.9015711
Produced by FastQC (version 0.11.5)