##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937097_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1818941 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.746316125701714 33.0 31.0 34.0 30.0 34.0 2 31.825700778639877 33.0 31.0 34.0 30.0 34.0 3 31.79840577566837 33.0 31.0 34.0 30.0 34.0 4 35.43786686868898 37.0 35.0 37.0 33.0 37.0 5 35.439289674596374 37.0 35.0 37.0 33.0 37.0 6 35.57489110421943 37.0 35.0 37.0 33.0 37.0 7 35.5179332369769 37.0 35.0 37.0 33.0 37.0 8 35.48827037270588 37.0 35.0 37.0 33.0 37.0 9 37.05142222864843 39.0 37.0 39.0 33.0 39.0 10-11 36.93012472642048 39.0 37.0 39.0 33.0 39.0 12-13 36.808988032047225 39.0 37.0 39.0 33.0 39.0 14-15 37.8790873370824 40.0 37.5 41.0 33.0 41.0 16-17 37.81989465298764 40.0 37.0 41.0 33.0 41.0 18-19 37.83846562367883 40.0 37.5 41.0 33.0 41.0 20-21 37.77229140472396 40.0 37.0 41.0 32.5 41.0 22-23 37.64111892579254 40.0 37.0 41.0 32.0 41.0 24-25 37.71105467412082 40.0 37.0 41.0 32.0 41.0 26-27 37.63889015641519 40.0 37.0 41.0 32.0 41.0 28-29 37.521916598724204 40.0 37.0 41.0 32.0 41.0 30-31 37.24533698454211 39.0 36.5 41.0 32.0 41.0 32-33 36.93748477823085 39.0 35.5 41.0 31.0 41.0 34-35 36.70391727934001 39.0 35.0 41.0 31.0 41.0 36-37 36.46111666073831 39.0 35.0 41.0 30.0 41.0 38-39 36.24875133388054 39.0 35.0 40.5 30.0 41.0 40-41 35.97659214894821 38.5 35.0 40.0 30.0 41.0 42-43 35.64181960822259 38.0 35.0 40.0 28.5 41.0 44-45 35.21116545286516 38.0 35.0 40.0 26.5 41.0 46-47 34.61933729571217 38.0 34.0 40.0 23.5 41.0 48-49 34.166837187132515 38.0 34.0 40.0 22.0 41.0 50-51 33.35890553899219 37.5 33.0 39.5 19.5 40.5 52-53 33.50607743736603 37.5 33.0 39.5 20.0 40.5 54-55 33.86953809936661 38.0 34.0 40.0 19.5 41.0 56-57 33.753894711263314 38.0 34.0 40.0 18.5 41.0 58-59 33.48558694317188 37.5 33.5 40.0 17.0 41.0 60-61 33.188699908353264 37.0 33.0 40.0 14.0 41.0 62-63 32.8374100644276 36.5 33.0 40.0 11.5 41.0 64-65 32.49406935134235 36.0 32.5 39.0 8.0 41.0 66-67 32.130158702233885 35.5 32.0 39.0 7.0 41.0 68-69 31.728914516743536 35.0 31.5 39.0 4.0 40.5 70-71 31.34509915384831 35.0 31.0 38.0 2.0 40.0 72-73 30.963065047189545 35.0 31.0 37.0 2.0 39.5 74-75 30.53365832096808 35.0 30.5 37.0 2.0 39.0 76-77 30.120485216397892 35.0 30.0 36.0 2.0 39.0 78-79 29.709277266277468 34.5 30.0 36.0 2.0 38.5 80-81 29.293090045251606 34.0 29.0 35.0 2.0 37.0 82-83 28.894798676812496 34.0 29.0 35.0 2.0 37.0 84-85 28.52070737863405 34.0 29.0 35.0 2.0 36.0 86-87 28.166872097555668 34.0 28.5 35.0 2.0 36.0 88-89 27.814584420275317 34.0 27.0 35.0 2.0 35.5 90-91 27.43193649491655 34.0 26.5 35.0 2.0 35.0 92-93 27.067899948376557 34.0 26.0 35.0 2.0 35.0 94-95 26.65955685203643 33.0 25.0 35.0 2.0 35.0 96-97 26.129163067960974 33.0 24.5 35.0 2.0 35.0 98-99 25.48200766270044 33.0 21.0 35.0 2.0 35.0 100-101 23.034904100792716 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4697916671803668 1101 2 0.45938897170153936 1101 3 0.3800149360788332 1101 4 0.3062191758185975 1101 5 0.4153404698789984 1101 6 0.2917752730961922 1101 7 0.27964595230569245 1101 8 0.21950284594326064 1101 9 0.450822070623353 1101 10-11 0.4357065546969494 1101 12-13 0.5604668768169603 1101 14-15 0.6254350310514099 1101 16-17 0.6823997680820071 1101 18-19 0.6894949861430248 1101 20-21 0.7409413395939595 1101 22-23 0.7195490932689168 1101 24-25 0.6654114144092205 1101 26-27 0.5666911832346528 1101 28-29 0.5451187887426414 1101 30-31 0.6721701840519003 1101 32-33 0.7907162192587052 1101 34-35 0.7865299749153394 1101 36-37 0.7065428169040118 1101 38-39 0.7340557298603869 1101 40-41 0.7430916127817895 1101 42-43 0.8550152503847883 1101 44-45 0.8025360911788297 1101 46-47 0.8595970261921053 1101 48-49 0.9473057717730526 1101 50-51 1.0236619201891202 1101 52-53 1.1465493515148708 1101 54-55 1.174883968568487 1101 56-57 0.9746890794172955 1101 58-59 0.907086556157175 1101 60-61 1.1445049166644239 1101 62-63 1.2199513299083051 1101 64-65 1.1670044342071222 1101 66-67 1.0559428430966804 1101 68-69 1.0408441304259206 1101 70-71 0.9599609501634738 1101 72-73 0.8674086824926839 1101 74-75 0.7798487070068774 1101 76-77 0.85619134136725 1101 78-79 0.9248718421814992 1101 80-81 0.9213656171918245 1101 82-83 1.0043626865420698 1101 84-85 0.8711025271433073 1101 86-87 0.886591312076451 1101 88-89 0.8672595135411179 1101 90-91 0.8731102690822397 1101 92-93 0.6881330339637408 1101 94-95 0.8248752421540999 1101 96-97 0.6672351002361161 1101 98-99 0.5133094218507672 1101 100-101 1.0814642512025863 1102 1 0.45520646582369295 1102 2 0.3980853188225062 1102 3 0.3326434885719145 1102 4 0.28568391403543103 1102 5 0.358183828812372 1102 6 0.31496394020469154 1102 7 0.24503229201953758 1102 8 0.3133637090675023 1102 9 0.473484442460709 1102 10-11 0.36573486743330363 1102 12-13 0.3283645708639753 1102 14-15 0.3823390016568382 1102 16-17 0.5297120073709252 1102 18-19 0.37405528593640014 1102 20-21 0.4400685666026547 1102 22-23 0.4785789556524449 1102 24-25 0.20184396089863554 1102 26-27 0.4558329320943528 1102 28-29 0.4242584908985023 1102 30-31 0.3653412592536469 1102 32-33 0.7009621648358717 1102 34-35 0.555547264689622 1102 36-37 0.3388817120737855 1102 38-39 0.5021727136037129 1102 40-41 0.7155549183059406 1102 42-43 0.6375200747581076 1102 44-45 0.5895144253568319 1102 46-47 0.7510047685803158 1102 48-49 0.8420418444407787 1102 50-51 0.8516072244657593 1102 52-53 0.9941807791395263 1102 54-55 1.110521224425959 1102 56-57 1.1398836624744888 1102 58-59 0.9280445597437819 1102 60-61 0.8107155419811747 1102 62-63 0.8926992247272167 1102 64-65 1.0244064360783938 1102 66-67 1.0782957728070244 1102 68-69 0.9911457755859523 1102 70-71 0.8860517945749997 1102 72-73 0.9235431899192932 1102 74-75 1.0043014573869833 1102 76-77 1.1229024345020235 1102 78-79 0.904416986620312 1102 80-81 0.8196298174179368 1102 82-83 0.8818735535647981 1102 84-85 0.8332117041734435 1102 86-87 0.7464749423442782 1102 88-89 0.9229006191510365 1102 90-91 0.6480532537611978 1102 92-93 0.49878978223866 1102 94-95 0.5534920602118731 1102 96-97 0.5161505908602635 1102 98-99 0.3822923562392937 1102 100-101 0.41535596107744865 1103 1 0.13088404587529467 1103 2 0.1822822592862039 1103 3 0.21044420625224092 1103 4 0.22211065885561965 1103 5 0.21712767926355525 1103 6 0.17911423832158135 1103 7 0.2240965120348477 1103 8 0.1869063505527251 1103 9 0.2777925294256036 1103 10-11 0.2459093780953907 1103 12-13 0.3167596948998508 1103 14-15 0.4567417653677026 1103 16-17 0.4409988881654172 1103 18-19 0.4778638667075157 1103 20-21 0.44611139140341294 1103 22-23 0.4442390863564256 1103 24-25 0.3196025675630736 1103 26-27 0.348387686153643 1103 28-29 0.3359184474527197 1103 30-31 0.3056418337187097 1103 32-33 0.3483070397917629 1103 34-35 0.2752291664049977 1103 36-37 0.30636783737912765 1103 38-39 0.36042268610637507 1103 40-41 0.1684495565556432 1103 42-43 0.25834234232546294 1103 44-45 0.3209504333402222 1103 46-47 0.42034936970862447 1103 48-49 0.5245633954735425 1103 50-51 0.4889397771804269 1103 52-53 0.45694380829127823 1103 54-55 0.3895914881253617 1103 56-57 0.32863407493444896 1103 58-59 0.3920303608097626 1103 60-61 0.448459265715492 1103 62-63 0.4399317616309233 1103 64-65 0.3790691526771539 1103 66-67 0.24738373218430354 1103 68-69 0.35993281105292496 1103 70-71 0.22013648291567378 1103 72-73 0.015616425621828967 1103 74-75 0.12929744735962245 1103 76-77 0.016272226622202624 1103 78-79 0.17286339890612012 1103 80-81 0.21087930757159867 1103 82-83 0.18396137430969972 1103 84-85 0.11526098855541989 1103 86-87 0.13312779542145847 1103 88-89 0.22184271664740152 1103 90-91 -0.04048018757553962 1103 92-93 0.13066597665395108 1103 94-95 0.1863454123656041 1103 96-97 0.0691312155537851 1103 98-99 0.21291896884315165 1103 100-101 0.25612994791539023 1104 1 0.14472364437652985 1104 2 0.14016998454720664 1104 3 0.19835703062590326 1104 4 0.07901606990662913 1104 5 0.12055647698668537 1104 6 0.10313230489569492 1104 7 0.08657687445428763 1104 8 0.18762004877152805 1104 9 0.18049158159955425 1104 10-11 0.295263758635798 1104 12-13 0.32099768605695544 1104 14-15 0.42359355657615794 1104 16-17 0.31036940307257055 1104 18-19 0.30003319922561644 1104 20-21 0.4926523213434919 1104 22-23 0.4393043962711758 1104 24-25 0.377034059061792 1104 26-27 0.4297420753976837 1104 28-29 0.415218370425535 1104 30-31 0.32669416324710454 1104 32-33 0.40496445159128314 1104 34-35 0.3499670237436874 1104 36-37 0.5198537690381642 1104 38-39 0.5314241265582709 1104 40-41 0.5638110349118719 1104 42-43 0.5267820848718827 1104 44-45 0.4955901299749641 1104 46-47 0.4881069103219673 1104 48-49 0.6109029286974916 1104 50-51 0.5527185318205525 1104 52-53 0.4870462881749944 1104 54-55 0.45038095150263047 1104 56-57 0.4652931404249614 1104 58-59 0.5172164010466744 1104 60-61 0.4887292253306228 1104 62-63 0.7059754201611455 1104 64-65 0.6851183978542537 1104 66-67 0.5367437537693149 1104 68-69 0.40318703014086665 1104 70-71 0.21726414677251782 1104 72-73 0.444140256179427 1104 74-75 0.41172039841471886 1104 76-77 0.43400109963178934 1104 78-79 0.43159777043826253 1104 80-81 0.4312509039056316 1104 82-83 0.41353489066601057 1104 84-85 0.49955456875290594 1104 86-87 0.517176585833532 1104 88-89 0.35618267090979217 1104 90-91 0.24649993331797404 1104 92-93 0.14619952047023688 1104 94-95 0.2840499913489296 1104 96-97 0.27950858892674546 1104 98-99 0.4214855777756945 1104 100-101 0.29341891192149916 1105 1 0.12658436387093985 1105 2 0.2236219603788605 1105 3 0.22077612222949128 1105 4 0.237994123938158 1105 5 0.2227206897792442 1105 6 0.1497935313697596 1105 7 0.07753978854554333 1105 8 0.16087339969867287 1105 9 0.29606818414139724 1105 10-11 0.2276136614068207 1105 12-13 0.2289144684723965 1105 14-15 0.15449717835544874 1105 16-17 0.268862614406693 1105 18-19 0.3990635138307894 1105 20-21 0.23353927529861807 1105 22-23 0.2668683764086808 1105 24-25 0.13542656895831584 1105 26-27 0.0788170979686953 1105 28-29 -0.009513815837401296 1105 30-31 -0.23681719170810567 1105 32-33 -0.06303896878512916 1105 34-35 -0.2556956958896279 1105 36-37 0.014894657294298952 1105 38-39 0.001187651803078893 1105 40-41 0.05393621721620434 1105 42-43 0.009183206477551664 1105 44-45 -0.20978437519899984 1105 46-47 -0.0629122576758121 1105 48-49 -0.04817061807658973 1105 50-51 0.01830207928913552 1105 52-53 -0.1699611428966108 1105 54-55 0.07675257453200857 1105 56-57 0.16499016799631505 1105 58-59 0.29506121665887264 1105 60-61 0.08813500003719099 1105 62-63 0.1445779272796912 1105 64-65 0.10696192865500365 1105 66-67 0.036205521947664465 1105 68-69 0.2427136465725681 1105 70-71 0.22644664208468868 1105 72-73 0.14227033465709837 1105 74-75 0.12196720374842229 1105 76-77 0.06192230943802457 1105 78-79 0.17060308330721696 1105 80-81 0.30311917600785776 1105 82-83 0.3266278137856702 1105 84-85 0.39104008858067374 1105 86-87 0.28584541700236343 1105 88-89 0.42924981098896353 1105 90-91 0.46791721924061136 1105 92-93 0.358840155236539 1105 94-95 0.35194750159269717 1105 96-97 0.14704496298049818 1105 98-99 0.0400050930198077 1105 100-101 0.19244401783163312 1106 1 0.044618602359722814 1106 2 0.1257492162398819 1106 3 0.18545186856107776 1106 4 0.29820874697745836 1106 5 0.24957808563014794 1106 6 0.27562630249473585 1106 7 0.25029236064876415 1106 8 0.33858043292519113 1106 9 0.27147297415657334 1106 10-11 0.2722670599563273 1106 12-13 0.37043496096691797 1106 14-15 0.5268723681007685 1106 16-17 0.3467214438888959 1106 18-19 0.11381621243089768 1106 20-21 0.32696354583328713 1106 22-23 0.24257570479458224 1106 24-25 0.33777835678088763 1106 26-27 0.20024777767859803 1106 28-29 0.3452603872322584 1106 30-31 0.19662963743677864 1106 32-33 0.13610190657327337 1106 34-35 0.08887758796937817 1106 36-37 0.0362178146564176 1106 38-39 -0.09762749408999127 1106 40-41 -0.1218807417988117 1106 42-43 -0.19235056818934027 1106 44-45 -0.17012865754004025 1106 46-47 -0.10748132497214868 1106 48-49 -0.2326895082772964 1106 50-51 -0.38866051980009075 1106 52-53 -0.24723492430999272 1106 54-55 -0.32046238583229325 1106 56-57 -0.2587764874261822 1106 58-59 -0.3294222544155119 1106 60-61 -0.2514943641051559 1106 62-63 -0.10398106243189176 1106 64-65 -0.12625615196566997 1106 66-67 -0.28459478164484864 1106 68-69 -0.3464603288027064 1106 70-71 -0.22049845826949976 1106 72-73 -0.21694917959488436 1106 74-75 -0.2102483891002862 1106 76-77 -0.3312299928051843 1106 78-79 -0.1926369234392844 1106 80-81 -0.3189966951339862 1106 82-83 -0.28860346467612885 1106 84-85 -0.08751025717746685 1106 86-87 0.11615617767026265 1106 88-89 0.026298217128278623 1106 90-91 -0.003034880607593493 1106 92-93 -0.195036974127607 1106 94-95 -0.12977053686235251 1106 96-97 -0.1876657808211526 1106 98-99 -0.12938477036356133 1106 100-101 -0.25892952745424935 1107 1 0.1835572952457838 1107 2 0.1955934697307491 1107 3 0.2008406151528348 1107 4 0.15720858172917218 1107 5 0.16067657360833465 1107 6 0.1265413648759619 1107 7 -0.00343605379394063 1107 8 0.15623927783967417 1107 9 0.31951065076567886 1107 10-11 0.280937430938728 1107 12-13 0.24838154445861704 1107 14-15 0.22657492118678135 1107 16-17 0.4003600045763278 1107 18-19 0.33261217211030925 1107 20-21 0.5592912744955996 1107 22-23 0.433354850460951 1107 24-25 0.30817412642477393 1107 26-27 0.2008684915511907 1107 28-29 0.44077079787424367 1107 30-31 0.3221873504005046 1107 32-33 0.18646855120683625 1107 34-35 0.200920056778763 1107 36-37 0.2511407676432782 1107 38-39 0.15176315285704334 1107 40-41 0.15261938213740933 1107 42-43 0.40138445618478613 1107 44-45 0.4173455224153386 1107 46-47 0.25671040435116055 1107 48-49 0.1177731869222427 1107 50-51 0.06490574476241306 1107 52-53 0.16838516988152463 1107 54-55 0.20213944842367226 1107 56-57 0.2932942801521037 1107 58-59 0.20600284679905911 1107 60-61 0.26131857483560594 1107 62-63 0.10073884172410175 1107 64-65 0.3141924971295893 1107 66-67 0.3025106880734043 1107 68-69 0.262297830331395 1107 70-71 0.17962444514287412 1107 72-73 0.19442093525267623 1107 74-75 0.24735303203496883 1107 76-77 0.31809765323298933 1107 78-79 0.23289187107446807 1107 80-81 0.2638587952402993 1107 82-83 0.18640066653101073 1107 84-85 0.1821511903975157 1107 86-87 0.1991459322765614 1107 88-89 0.15876640683330834 1107 90-91 0.06722042799357553 1107 92-93 -0.05723539600647953 1107 94-95 -0.02041025428778198 1107 96-97 -0.12440667150941209 1107 98-99 -0.23083172566390076 1107 100-101 -0.1581735165478655 1108 1 -0.07857434015592801 1108 2 0.09389500044997945 1108 3 0.06853179375825036 1108 4 0.22706881690184133 1108 5 0.2266656537251137 1108 6 0.08948312012218906 1108 7 -0.12077439274582247 1108 8 0.1100354292663539 1108 9 0.032313286377082306 1108 10-11 -0.07140925519124863 1108 12-13 0.04248045247029353 1108 14-15 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-1.3201443512525444 2316 66-67 -1.2376946441300944 2316 68-69 -1.0663258868327183 2316 70-71 -1.0276849733048543 2316 72-73 -1.7958087198331611 2316 74-75 -1.8643694203392585 2316 76-77 -1.8468350273055663 2316 78-79 -1.9396593639002653 2316 80-81 -2.192448285072288 2316 82-83 -1.722343409670227 2316 84-85 -1.6768937833965438 2316 86-87 -2.0371136007240978 2316 88-89 -2.2552227812531846 2316 90-91 -2.0213835170210146 2316 92-93 -2.632397025482671 2316 94-95 -2.2123825262019174 2316 96-97 -2.2865775279887153 2316 98-99 -2.403853726103282 2316 100-101 -2.6539268197522077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3031.0 3 1126.0 4 1209.0 5 1230.0 6 1534.0 7 1918.0 8 2286.0 9 3132.0 10 4007.0 11 5636.0 12 8426.0 13 10781.0 14 12679.0 15 16802.0 16 22685.0 17 30401.0 18 25626.0 19 13247.0 20 12580.0 21 12536.0 22 13310.0 23 14341.0 24 16238.0 25 18337.0 26 21439.0 27 25093.0 28 30150.0 29 36800.0 30 44815.0 31 56035.0 32 71069.0 33 92894.0 34 126490.0 35 180318.0 36 251609.0 37 328552.0 38 260401.0 39 40170.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.571631873340706 18.937745937968092 14.86884041554031 20.62178177315089 2 15.630364284763864 21.22170159373897 40.07533778842738 23.072596333069782 3 18.56092742505277 27.698048015272335 30.493761247651495 23.247263312023392 4 12.349243723540496 17.318722674436827 37.73618002167624 32.595853580346436 5 12.976359813994012 38.5337012089069 32.685150654068245 15.804788323030838 6 29.35619716396543 37.1225958246505 17.894692843585812 15.626514167798252 7 26.86830763884536 35.57430177304614 20.622782042780525 16.934608545327983 8 27.38617350929125 32.64533083410609 19.944207391860573 20.024288264742086 9 28.746379509356494 15.722181681403422 20.350195686591302 35.18124312264877 10-11 24.728053466596574 27.31142180939163 26.411394535004696 21.5491301890071 12-13 26.817050669702397 25.437636972081755 27.43382031462676 20.311492043589087 14-15 23.78243631233877 25.3211567112783 27.627381508466716 23.269025467916222 16-17 21.122886004092038 29.252171799678113 27.50725399512716 22.117688201102684 18-19 21.000090175986134 26.23224935199756 32.201129839123844 20.56653063289246 20-21 22.67334465820701 25.01750553482811 32.240287830306144 20.068861976658738 22-23 22.094556646650883 25.38538152186493 32.16424764592541 20.355814185558778 24-25 21.33852947418075 25.747561457001268 32.388941973067446 20.52496709575054 26-27 21.263828788289448 25.84162982746554 32.24356919768151 20.6509721865635 28-29 20.90554730881302 25.855462092473473 32.42085876078949 20.81813183792402 30-31 21.186503575432077 25.69305436514983 32.34313262497244 20.777309434445648 32-33 20.966638399170943 26.04079590639015 32.10448985874957 20.88807583568934 34-35 21.35038026278499 25.95482147701999 31.908812799985924 20.785985460209094 36-37 21.5974844703594 25.78706511096292 31.52936241472373 21.086088003953947 38-39 21.392207980700825 25.963254382502317 31.551212850575443 21.09332478622141 40-41 21.379771394202884 25.914145554084 31.582807339883306 21.12327571182981 42-43 21.382751913479183 26.152760566880236 31.025301577074092 21.439185942566485 44-45 21.419784901900147 26.29675291207092 30.847321581967496 21.43614060406144 46-47 21.67346870334681 26.73273259900475 30.053017127278213 21.540781570370225 48-49 21.918308285723082 27.066262914143213 29.12818262984514 21.887246170288567 50-51 21.89856284350382 27.236207481394377 28.85863682069602 22.00659285440578 52-53 22.066935524438964 27.42277806808895 28.578567778465302 21.931718629006784 54-55 21.703423274041153 27.362386758604917 28.642169836527753 22.292020130826177 56-57 21.808560790463638 27.700560631029465 28.326697114928674 22.164181463578224 58-59 22.085004194792297 27.166997815189436 28.527638898204543 22.220359091813723 60-61 22.09727995372988 27.27732299659489 28.91535993626863 21.710037113406596 62-63 22.01709598126787 27.218818774131936 29.075428265436333 21.688656979163863 64-65 21.890062174291433 27.36958050358482 28.92127137052282 21.819085951600922 66-67 21.84623293649648 27.693961399471895 28.532748318563634 21.92705734546799 68-69 21.92123569276922 27.43760737382021 28.740595297366887 21.900561636043676 70-71 21.927439007122036 27.577059589669904 28.515299463324812 21.98020193988325 72-73 21.92279459270725 27.79180218348175 28.10002446863204 22.185378755178963 74-75 21.732958841244642 28.11544444450554 28.061915463249754 22.08968125100006 76-77 21.767870061215046 27.893939014823538 28.175776241589222 22.162414682372194 78-79 21.730859747018084 27.90419481059348 28.010483727293323 22.354461715095116 80-81 21.67362794749834 28.317351904571236 27.871349590133278 22.137670557797147 82-83 21.694919075937648 28.06264833236679 27.806398196597392 22.43603439509817 84-85 21.84018587602821 27.844618329771585 27.595942333562352 22.719253460637848 86-87 21.784206926281247 27.83758253166005 27.675653325239622 22.702557216819084 88-89 21.7964712564489 27.80847089795521 27.61160177949229 22.783456066103597 90-91 21.75685741002807 27.850393079448153 27.730845676895928 22.661903833627846 92-93 21.751924607200852 27.7541584108868 27.621900954838342 22.872016027074007 94-95 21.766026942478707 27.58454885877563 27.729292997050408 22.920131201695252 96-97 21.807240972977908 27.57754080972252 27.622354935574883 22.99286328172469 98-99 21.662694920096268 27.52710452388671 27.561666555309287 23.24853400070774 100-101 21.961099522021073 27.48021345435607 27.500067371324892 23.058619652297967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2464.0 1 1711.5 2 1108.0 3 1283.0 4 1176.0 5 1299.0 6 2170.0 7 4122.5 8 6671.5 9 7654.5 10 6294.0 11 4980.5 12 4864.5 13 5125.5 14 5371.0 15 5486.0 16 5628.0 17 5755.5 18 5896.5 19 6149.0 20 6465.0 21 6992.0 22 7882.5 23 9135.5 24 10267.0 25 11716.0 26 13802.0 27 15470.0 28 17035.5 29 19000.0 30 21759.5 31 24955.0 32 28804.0 33 32678.0 34 36042.5 35 38047.5 36 41442.0 37 46182.0 38 47835.0 39 48605.0 40 49256.5 41 50506.5 42 51705.0 43 53688.5 44 56694.5 45 58596.0 46 58519.0 47 58610.5 48 62321.0 49 62007.5 50 58230.0 51 55861.0 52 53922.5 53 54665.5 54 55273.5 55 53588.5 56 51231.5 57 47672.0 58 44526.0 59 41521.5 60 36793.5 61 31715.5 62 26936.0 63 21079.0 64 15664.5 65 12048.5 66 8524.5 67 6332.0 68 4447.0 69 2620.5 70 1809.5 71 1270.5 72 896.0 73 656.5 74 517.0 75 430.5 76 317.0 77 154.5 78 66.0 79 40.5 80 24.5 81 16.0 82 12.5 83 11.5 84 12.0 85 8.5 86 7.0 87 6.5 88 7.0 89 5.0 90 3.5 91 3.0 92 3.5 93 4.5 94 2.0 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02160597842370918 2 0.010940431822692434 3 0.012534766108411434 4 0.01979173596064963 5 0.0514035364533539 6 0.03837397694592623 7 0.014458962660141258 8 0.04282711753707239 9 0.02594916492618507 10-11 0.021166162069028077 12-13 0.03276631842374217 14-15 0.017565165665076547 16-17 0.015833388768519707 18-19 0.015118687192162913 20-21 0.012672208719249277 22-23 0.00695459610839494 24-25 0.0014843801970487224 26-27 0.0 28-29 0.0022540588177406523 30-31 0.0 32-33 4.6730487684867183E-4 34-35 8.24655665027068E-5 36-37 0.0 38-39 1.649311330054136E-4 40-41 1.0995408867027572E-4 42-43 1.649311330054136E-4 44-45 1.9241965517298253E-4 46-47 2.748852216756893E-5 48-49 2.7488522167568936E-4 50-51 1.0995408867027572E-4 52-53 6.322360098540854E-4 54-55 0.0021441047290703765 56-57 4.6730487684867183E-4 58-59 0.0010445638423676195 60-61 0.0031062030049352892 62-63 0.0018692195073946873 64-65 0.001209494975373033 66-67 0.010088287635497798 68-69 0.013002070985260105 70-71 0.023612640541941714 72-73 0.015723434679849428 74-75 0.01649311330054136 76-77 0.028340666354763566 78-79 0.017207814876898153 80-81 0.04337688798042377 82-83 0.033013715123250284 84-85 0.01701539522172517 86-87 0.013469375862108775 88-89 0.01209494975373033 90-91 0.02284296192124978 92-93 0.028478108965601413 94-95 0.011792576009887071 96-97 0.01748270009857384 98-99 0.025921676404017505 100-101 0.03628484926119099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1818941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.114502247461296 #Duplication Level Percentage of deduplicated Percentage of total 1 68.8573484462881 28.998929558954256 2 14.689522207597467 12.372838320519921 3 6.0952457475767865 7.700947222054542 4 3.1159157732431604 5.249009673405967 5 1.8465830512275077 3.88839630305224 6 1.1787517221013346 2.9785525229781316 7 0.7808699252588288 2.302016375560151 8 0.5827675706767306 1.9634372932010182 9 0.4139847935579203 1.569128716683885 >10 2.170262375569894 16.556627527356078 >50 0.17040332420921953 4.971097831607193 >100 0.0930933051088002 7.012079132119567 >500 0.003151054550581393 0.8589337206064767 >1k 0.0017068212148982544 1.159897814932539 >5k 1.3129393960755804E-4 0.5188769173536228 >10k+ 2.625878792151161E-4 1.8992310696144754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20160 1.1083372137963792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14188 0.7800143050269359 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9384 0.5159045840409336 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3045 0.16740510000049477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2954 0.16240218896599726 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2359 0.12969084758659022 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2178 0.11974000256193026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.497704433513786E-5 0.0 10-11 0.0 0.0 0.0 8.24655665027068E-5 0.0 12-13 0.0 0.0 0.0 1.0995408867027572E-4 0.0 14-15 0.0 0.0 0.0 1.649311330054136E-4 0.0 16-17 0.0 0.0 0.0 2.1990817734055145E-4 0.0 18-19 0.0 0.0 0.0 2.748852216756893E-4 0.0 20-21 0.0 0.0 0.0 4.6730487684867183E-4 0.0 22-23 0.0 0.0 0.0 9.071212315297747E-4 0.0 24-25 0.0 0.0 0.0 0.0017592654187244116 0.0 26-27 0.0 0.0 0.0 0.0026388981280866175 0.0 28-29 0.0 0.0 0.0 0.005580170000016493 0.0 30-31 0.0 0.0 0.0 0.010748012167519451 0.0 32-33 0.0 0.0 0.0 0.019709270394146924 0.0 34-35 0.0 0.0 0.0 0.02883545975377981 0.0 36-37 0.0 0.0 0.0 0.038044114679915406 0.0 38-39 0.0 0.0 0.0 0.04997413330064032 0.0 40-41 0.0 0.0 0.0 0.06987582334996023 0.0 42-43 0.0 0.0 0.0 0.09678708655201021 0.0 44-45 0.0 0.0 0.0 0.12251634330085473 0.0 46-47 0.0 0.0 0.0 0.15357837335020763 0.0 48-49 0.0 0.0 0.0 0.1881864227591769 0.0 50-51 0.0 0.0 0.0 0.2304087928085628 0.0 52-53 0.0 2.748852216756893E-5 0.0 0.29682106236540934 0.0 54-55 0.0 5.497704433513786E-5 0.0 0.379039232168608 0.0 56-57 0.0 5.497704433513786E-5 0.0 0.4546876451737577 0.0 58-59 0.0 5.497704433513786E-5 0.0 0.5542235839425248 0.0 60-61 0.0 5.497704433513786E-5 0.0 0.6715171080315414 0.0 62-63 0.0 5.497704433513786E-5 0.0 0.7722075647313464 0.0 64-65 0.0 5.497704433513786E-5 0.0 0.8740250508400218 0.0 66-67 0.0 5.497704433513786E-5 0.0 1.0240024277862778 0.0 68-69 0.0 5.497704433513786E-5 0.0 1.1871468068507993 0.0 70-71 0.0 5.497704433513786E-5 0.0 1.3182945461122708 0.0 72-73 0.0 5.497704433513786E-5 0.0 1.4992514875413772 0.0 74-75 0.0 5.497704433513786E-5 0.0 1.700577423896652 0.0 76-77 0.0 5.497704433513786E-5 0.0 1.9107821529120517 0.0 78-79 0.0 5.497704433513786E-5 0.0 2.1315149859176303 0.0 80-81 0.0 5.497704433513786E-5 0.0 2.386938333898681 0.0 82-83 0.0 5.497704433513786E-5 0.0 2.676914754244365 0.0 84-85 0.0 5.497704433513786E-5 0.0 2.9721964593683907 0.0 86-87 0.0 5.497704433513786E-5 0.0 3.2955989226698392 0.0 88-89 0.0 5.497704433513786E-5 0.0 3.6625981821290523 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8230 0.0 53.295677 1 GTATCAA 15670 0.0 45.277344 1 TATCAAC 20040 0.0 35.215252 2 ATCAACG 20270 0.0 34.815674 3 TCAACGC 20370 0.0 34.644756 4 CAACGCA 20765 0.0 33.98573 5 AACGCAG 21380 0.0 33.139664 6 ACGCAGA 24120 0.0 29.09922 7 CGCAGAG 24075 0.0 28.976765 8 GCAGAGT 24980 0.0 27.390532 9 GTACATG 15085 0.0 25.73753 1 GAGTACT 13920 0.0 25.155605 12-13 TACATGG 15470 0.0 24.913378 2 ACATGGG 15640 0.0 23.670246 3 GTACTTT 15110 0.0 22.825113 14-15 AGTACTT 14260 0.0 22.773275 12-13 CAGAGTA 24585 0.0 22.07152 10-11 AGAGTAC 23030 0.0 21.839027 10-11 CATGGGA 10760 0.0 21.553095 4 GTGGTAT 3615 0.0 20.901571 1 >>END_MODULE