FastQCFastQC Report
Thu 26 May 2016
SRR937097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818941
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198221.0897549728111027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157920.8681974841404972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108850.5984251275879756No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34530.18983573408923105No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32360.17790571546850614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.15816895655219163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28080.15437554049306712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88900.053.498971
GTATCAA158350.041.7370951
TATCAAC213600.030.9978352
ATCAACG214100.030.9237443
TCAACGC216150.030.6084844
CAACGCA220100.030.0466825
AACGCAG224800.029.3973466
ACGCAGA257900.025.4769947
CGCAGAG257950.025.3615478
GCAGAGT267150.024.0435649
GTACATG142700.023.739891
GAGTACT154450.023.47767812-13
TAAGGTG6300.023.3775225
GTAAGGT5900.023.3448964
TACATGG147300.022.7652192
GTGGTAT39500.022.613821
AGTACTT157850.022.31005312-13
TGGTATC40100.022.1496622
GTACTTT163300.022.14714614-15
GGACCGT1754.044625E-621.7184726
Produced by FastQC (version 0.11.5)