##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937097_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1818941 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.783796725677192 31.0 31.0 34.0 30.0 34.0 2 31.888382305968143 31.0 31.0 34.0 30.0 34.0 3 31.964770709990045 31.0 31.0 34.0 30.0 34.0 4 35.738060222953905 37.0 35.0 37.0 35.0 37.0 5 35.62233134554667 37.0 35.0 37.0 33.0 37.0 6 35.678107206335994 37.0 35.0 37.0 33.0 37.0 7 35.60345607691509 37.0 35.0 37.0 33.0 37.0 8 35.569988251405626 37.0 35.0 37.0 33.0 37.0 9 37.143075558800426 39.0 37.0 39.0 33.0 39.0 10-11 37.04870581288783 39.0 37.0 39.0 33.0 39.0 12-13 36.92150267655741 39.0 37.0 39.0 33.0 39.0 14-15 37.916642980723395 40.0 37.0 41.0 33.0 41.0 16-17 37.83769347109114 39.0 37.0 41.0 33.0 41.0 18-19 38.072759644210564 40.0 37.0 41.0 33.0 41.0 20-21 38.1320218742664 40.0 37.0 41.0 33.0 41.0 22-23 38.03415998649764 40.0 37.0 41.0 33.0 41.0 24-25 37.89130900892333 40.0 37.0 41.0 33.0 41.0 26-27 37.64466329584082 40.0 36.5 41.0 32.0 41.0 28-29 37.5001242481202 39.0 36.0 41.0 32.0 41.0 30-31 37.34728201739364 39.0 36.0 41.0 32.0 41.0 32-33 37.048267645844476 39.0 35.0 41.0 31.0 41.0 34-35 36.81342907768861 39.0 35.0 41.0 31.0 41.0 36-37 36.553050648701635 39.0 35.0 41.0 30.5 41.0 38-39 36.32251430915021 39.0 35.0 40.0 30.0 41.0 40-41 36.0263551703986 38.0 35.0 40.0 30.0 41.0 42-43 35.61788122869296 38.0 35.0 40.0 28.5 41.0 44-45 35.15333757389602 38.0 34.5 40.0 26.5 41.0 46-47 34.54762771304841 38.0 34.0 40.0 23.5 41.0 48-49 33.97282787072258 38.0 33.0 40.0 21.5 41.0 50-51 33.57922576928004 38.0 33.0 40.0 19.5 41.0 52-53 33.269287459021484 37.5 33.0 40.0 17.5 41.0 54-55 32.93035178161359 37.0 32.5 40.0 14.5 41.0 56-57 32.599916105030346 37.0 32.0 40.0 12.0 41.0 58-59 32.19457173157348 36.0 31.0 39.0 8.5 41.0 60-61 31.815479721442312 36.0 31.0 39.0 7.5 40.0 62-63 31.404079901437157 35.0 30.5 39.0 6.5 40.0 64-65 30.978760443576782 35.0 30.0 38.0 2.0 40.0 66-67 30.555952611986868 35.0 29.0 38.0 2.0 40.0 68-69 30.239642462289872 34.5 29.0 37.5 2.0 40.0 70-71 29.751544717503208 34.0 29.0 37.0 2.0 39.0 72-73 29.236223165017446 34.0 28.0 36.0 2.0 39.0 74-75 28.698889903520787 34.0 27.0 36.0 2.0 38.5 76-77 27.389227028254354 31.5 26.0 34.5 2.0 36.5 78-79 28.135565694544244 33.0 27.0 35.0 2.0 37.0 80-81 27.927534757861856 33.0 26.5 35.0 2.0 37.0 82-83 27.571135072550454 33.0 26.0 35.0 2.0 36.0 84-85 27.17666625800397 33.0 26.0 35.0 2.0 36.0 86-87 26.75905540641505 33.0 25.0 35.0 2.0 35.0 88-89 26.304374633371836 32.0 24.5 35.0 2.0 35.0 90-91 25.75418416540174 32.0 23.5 34.0 2.0 35.0 92-93 25.174548542256183 32.0 20.0 34.0 2.0 35.0 94-95 24.476653998123084 31.0 16.5 34.0 2.0 35.0 96-97 23.66954645587735 31.0 4.5 34.0 2.0 35.0 98-99 22.675733297561603 31.0 2.0 34.0 2.0 35.0 100-101 19.27870805045353 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2289401432241469 1101 2 0.2701940932957463 1101 3 0.26050421099225574 1101 4 0.23863144614168164 1101 5 0.2460431106334653 1101 6 0.30497336576362954 1101 7 0.34446157155171164 1101 8 0.3614481679714814 1101 9 0.3657202755540112 1101 10-11 0.43267403925963777 1101 12-13 0.44081128301908734 1101 14-15 0.5575075536560021 1101 16-17 0.43733815589521186 1101 18-19 0.4067270522221378 1101 20-21 0.4689139849450825 1101 22-23 0.4642260919246226 1101 24-25 0.4430038616682026 1101 26-27 0.41573583556073856 1101 28-29 0.46108566997299505 1101 30-31 0.4824192708201238 1101 32-33 0.625835993669476 1101 34-35 0.6274186321151305 1101 36-37 0.4547986620135731 1101 38-39 0.46593485854162253 1101 40-41 0.7201416293957834 1101 42-43 0.5843712451614209 1101 44-45 0.6301550347182712 1101 46-47 0.6328667194547322 1101 48-49 0.6145044660572907 1101 50-51 0.5155313942030091 1101 52-53 0.5239633427918875 1101 54-55 0.38166689204580706 1101 56-57 0.6108998222086868 1101 58-59 0.5882580058440752 1101 60-61 0.6134824106023551 1101 62-63 0.6365371154312953 1101 64-65 0.4950668345489113 1101 66-67 0.3360792169675939 1101 68-69 0.4641774270062271 1101 70-71 0.31239193447773417 1101 72-73 0.3808412456300161 1101 74-75 0.2659211690637484 1101 76-77 0.16561564219947655 1101 78-79 0.4534952232153948 1101 80-81 0.6244654840534913 1101 82-83 0.5802055282792757 1101 84-85 0.43024445667391475 1101 86-87 0.08727953012454392 1101 88-89 0.2244817580683467 1101 90-91 0.5599175330577957 1101 92-93 0.6212300945369229 1101 94-95 0.4908662100188188 1101 96-97 0.6676086221321178 1101 98-99 0.8889152414872434 1101 100-101 1.1878312301204907 1102 1 0.24282361755263437 1102 2 0.25592808027481695 1102 3 0.32011202355316826 1102 4 0.22968198998012923 1102 5 0.2637202967080867 1102 6 0.3010090953648188 1102 7 0.2297560045172844 1102 8 0.19249276662260684 1102 9 0.3530721331813922 1102 10-11 0.37781750345206433 1102 12-13 0.2847220954630387 1102 14-15 0.4229501777252338 1102 16-17 0.5392579153587747 1102 18-19 0.5219747521168756 1102 20-21 0.42511939604016646 1102 22-23 0.28753341912433683 1102 24-25 0.44868955618304085 1102 26-27 0.4232720914571999 1102 28-29 0.476750263945938 1102 30-31 0.5541525853107814 1102 32-33 0.4858597256877246 1102 34-35 0.49252866681163 1102 36-37 0.29031311564111206 1102 38-39 0.5758940219270627 1102 40-41 0.5166127708717525 1102 42-43 0.46997877508228214 1102 44-45 0.5406799655201837 1102 46-47 0.5025635636323571 1102 48-49 0.5558798023014901 1102 50-51 0.7368775405520793 1102 52-53 0.7157577660112509 1102 54-55 0.7050838640936874 1102 56-57 0.659622337900089 1102 58-59 0.8275371762467856 1102 60-61 0.7887180251982748 1102 62-63 0.7232016216883608 1102 64-65 0.6596876095674276 1102 66-67 0.757013098687608 1102 68-69 0.7473900697881888 1102 70-71 0.7048242470581414 1102 72-73 0.9587195447216672 1102 74-75 1.0217871684399924 1102 76-77 0.6885033951135924 1102 78-79 0.6969250602587884 1102 80-81 0.8807444790244503 1102 82-83 0.8134310847877977 1102 84-85 0.8128757381054399 1102 86-87 0.6416633748089602 1102 88-89 0.7489703257655087 1102 90-91 0.8401112876872467 1102 92-93 0.6739534289098508 1102 94-95 0.6363442128257226 1102 96-97 0.9461248105848092 1102 98-99 1.1797737850138112 1102 100-101 1.1587668174270256 1103 1 0.3079372960996771 1103 2 0.2534404311590812 1103 3 0.2457672620237652 1103 4 0.19802883901520119 1103 5 0.19733515305744476 1103 6 0.28343343187714964 1103 7 0.13502225887675934 1103 8 0.14279248752470153 1103 9 0.23708237822843614 1103 10-11 0.3296685320813708 1103 12-13 0.3415792708914296 1103 14-15 0.46585618153819297 1103 16-17 0.4476206269163612 1103 18-19 0.45069379470565707 1103 20-21 0.4578944953742692 1103 22-23 0.32514274965600265 1103 24-25 0.39589940778910915 1103 26-27 0.4398206150796895 1103 28-29 0.49892342380167065 1103 30-31 0.4261148463704316 1103 32-33 0.5549119671784482 1103 34-35 0.6315500139058443 1103 36-37 0.5194889003461753 1103 38-39 0.34933664187271063 1103 40-41 0.6641939510113488 1103 42-43 0.7207631650769173 1103 44-45 0.5454616367162686 1103 46-47 0.5102380399227755 1103 48-49 0.5674912661676998 1103 50-51 0.6002160945591299 1103 52-53 0.5143571493836134 1103 54-55 0.6449141196355441 1103 56-57 0.5182738859997897 1103 58-59 0.39176492517782435 1103 60-61 0.4390592498835453 1103 62-63 0.4735526422976051 1103 64-65 0.5244588890640429 1103 66-67 0.289822569684798 1103 68-69 0.19035086776071708 1103 70-71 0.31164579052913055 1103 72-73 0.3010603065352129 1103 74-75 0.617898215263363 1103 76-77 0.5957152288189036 1103 78-79 0.6000881984832809 1103 80-81 0.6448869862310396 1103 82-83 0.5486960960387854 1103 84-85 0.7360642368886836 1103 86-87 0.5214464781576744 1103 88-89 0.4950887624964402 1103 90-91 0.6167607837986644 1103 92-93 0.5407513475910797 1103 94-95 0.2103582903132022 1103 96-97 0.39352227418521935 1103 98-99 0.365688392054043 1103 100-101 0.5238664895213603 1104 1 0.20261948280138853 1104 2 0.23118278392618308 1104 3 0.198277227687921 1104 4 0.15826736218210868 1104 5 0.19532678886246657 1104 6 0.014448896063861127 1104 7 0.1481718221717827 1104 8 0.12572601567444508 1104 9 0.23050297277386278 1104 10-11 0.18214188807317555 1104 12-13 0.16339624211285297 1104 14-15 0.06148711905044024 1104 16-17 0.2162351221707084 1104 18-19 0.26725948105269737 1104 20-21 0.17321376653172393 1104 22-23 0.12812518680486562 1104 24-25 0.17668185929898073 1104 26-27 0.03764704497842075 1104 28-29 0.16300928082702626 1104 30-31 0.3211103639974766 1104 32-33 0.18720575082344482 1104 34-35 0.1002083537306433 1104 36-37 0.1072611337864231 1104 38-39 0.3252479670449304 1104 40-41 0.20978550456699452 1104 42-43 0.08832998724856367 1104 44-45 -0.07810117525470872 1104 46-47 -0.1084835100643815 1104 48-49 0.12313225638046532 1104 50-51 0.31036744536356053 1104 52-53 0.2939699137021847 1104 54-55 0.13291860418441814 1104 56-57 0.1631616961212501 1104 58-59 0.15139658726783267 1104 60-61 0.33638897410881086 1104 62-63 0.35681878536798806 1104 64-65 0.22695865515380476 1104 66-67 0.13726984740479153 1104 68-69 0.19393948601485533 1104 70-71 0.2387607198773587 1104 72-73 0.2677406088995511 1104 74-75 0.2506044696967784 1104 76-77 0.004538105457903896 1104 78-79 0.25531633712351365 1104 80-81 0.448584889524934 1104 82-83 0.5689713539928256 1104 84-85 0.499167290124479 1104 86-87 0.3395978583368837 1104 88-89 0.2817146181727388 1104 90-91 0.5431142809199336 1104 92-93 0.4016598875995214 1104 94-95 0.556902701708001 1104 96-97 0.6047777629161821 1104 98-99 0.4350839843147689 1104 100-101 0.4583317761538801 1105 1 0.16823775490361825 1105 2 0.22003617652289265 1105 3 0.16517163007697278 1105 4 0.2105887039419514 1105 5 0.17558583462953692 1105 6 0.16267533539992485 1105 7 0.24725856430665516 1105 8 0.10012093893300289 1105 9 0.20870458399686953 1105 10-11 0.2114494788780945 1105 12-13 0.18287676159337707 1105 14-15 0.17072864623983008 1105 16-17 0.1852390460543063 1105 18-19 0.15723447931646461 1105 20-21 0.24639810224948633 1105 22-23 0.26453873982937637 1105 24-25 0.07126004181165513 1105 26-27 0.2833064657715383 1105 28-29 0.20342875440157826 1105 30-31 0.28205383229389014 1105 32-33 0.2816502434965429 1105 34-35 0.4353813692178292 1105 36-37 0.3948028033468063 1105 38-39 0.28148624748107665 1105 40-41 0.43718844921593103 1105 42-43 0.5230715491625801 1105 44-45 0.41143802885668634 1105 46-47 0.39181381961129347 1105 48-49 0.29058963189265086 1105 50-51 0.3296287655941299 1105 52-53 0.5294051001734701 1105 54-55 0.3870551761685803 1105 56-57 0.38086127595317976 1105 58-59 0.6201530981366403 1105 60-61 0.4895723548991491 1105 62-63 0.49354051951619127 1105 64-65 0.15854027540521187 1105 66-67 0.31297649369967573 1105 68-69 0.41989701778789623 1105 70-71 0.07103639874394574 1105 72-73 0.13416796496594685 1105 74-75 0.4440792769750601 1105 76-77 0.20114464063395943 1105 78-79 0.49646155554146176 1105 80-81 0.3510737776421635 1105 82-83 0.4192669474368138 1105 84-85 0.6575376630670426 1105 86-87 0.4509727802064596 1105 88-89 0.4457472766906925 1105 90-91 0.37585006118245445 1105 92-93 0.3490933031144863 1105 94-95 0.31092641863278914 1105 96-97 0.2583322632287057 1105 98-99 -0.13643790956674806 1105 100-101 0.2798905649586594 1106 1 0.19025453682299798 1106 2 0.17900691357804632 1106 3 0.08964877630736723 1106 4 0.09998404885677559 1106 5 0.10763171083125656 1106 6 0.20344949630395348 1106 7 0.12251155806613667 1106 8 0.19536887281729776 1106 9 0.1731800436021942 1106 10-11 0.12004705013799821 1106 12-13 0.19477379313326537 1106 14-15 0.2506377793145447 1106 16-17 0.2695439457001214 1106 18-19 -0.02751843011176902 1106 20-21 0.10577679174181043 1106 22-23 0.05365789989009784 1106 24-25 0.11594506458108356 1106 26-27 0.18422567643100507 1106 28-29 0.33338743208752675 1106 30-31 0.30104733878739154 1106 32-33 0.2602174555053196 1106 34-35 0.1855099743789026 1106 36-37 0.315521938108148 1106 38-39 0.4223067905743463 1106 40-41 0.47557181909280644 1106 42-43 0.3954878503938204 1106 44-45 0.3471839387909057 1106 46-47 0.3818901393954022 1106 48-49 0.39097439323520433 1106 50-51 0.3540574213539571 1106 52-53 0.3230665523576448 1106 54-55 0.24631896432847356 1106 56-57 0.4730806637082807 1106 58-59 0.3774920254430967 1106 60-61 0.45309065478108224 1106 62-63 0.4697464564364182 1106 64-65 0.21551657832307214 1106 66-67 0.36420562171451465 1106 68-69 0.47648750522253636 1106 70-71 0.35534235253041757 1106 72-73 0.36554973254901313 1106 74-75 0.4243889835023005 1106 76-77 0.362371238711205 1106 78-79 0.39830103100106484 1106 80-81 0.4791371104132871 1106 82-83 0.5276073083745736 1106 84-85 0.4637381184558116 1106 86-87 0.34219389675225287 1106 88-89 0.4097083156517307 1106 90-91 0.515210619455349 1106 92-93 0.6927463221733525 1106 94-95 0.6291641062698297 1106 96-97 0.4374908481502757 1106 98-99 0.40667011876855597 1106 100-101 0.3808782947613203 1107 1 0.05331947939922088 1107 2 0.08996080930637973 1107 3 0.2263855357884843 1107 4 0.09521663581868722 1107 5 0.19178853145424313 1107 6 0.15484584431684567 1107 7 0.12723146222213444 1107 8 0.1268466056108224 1107 9 0.1153947837492666 1107 10-11 0.22675922893664335 1107 12-13 0.15699803505675192 1107 14-15 0.16054131562786012 1107 16-17 0.1823186709748441 1107 18-19 0.21651610677990618 1107 20-21 0.1319263001281641 1107 22-23 0.18371346462611626 1107 24-25 0.19498022115384117 1107 26-27 0.09386791389279381 1107 28-29 0.20960200698631581 1107 30-31 0.25727926475615703 1107 32-33 0.22934907576475183 1107 34-35 0.25855281499693206 1107 36-37 0.15079934866751898 1107 38-39 0.3547894395043656 1107 40-41 0.3231798501594838 1107 42-43 0.3558447866906107 1107 44-45 0.21994172611636031 1107 46-47 0.20435564381579496 1107 48-49 0.31104689592049084 1107 50-51 0.10972337194471748 1107 52-53 0.1397091203160059 1107 54-55 0.2818401161090165 1107 56-57 0.2914128953863795 1107 58-59 0.33252177013449824 1107 60-61 0.3465189370251487 1107 62-63 0.36184655558538736 1107 64-65 0.28950649817765495 1107 66-67 0.36494097658520985 1107 68-69 0.4963286603080519 1107 70-71 0.422284433006336 1107 72-73 0.3279165011187892 1107 74-75 0.24334296185477555 1107 76-77 0.28017983988446815 1107 78-79 0.4694755331906073 1107 80-81 0.7565259809412588 1107 82-83 0.5468370448035635 1107 84-85 0.5593197924209434 1107 86-87 0.5128767852922813 1107 88-89 0.5311585759177788 1107 90-91 0.5238314159520847 1107 92-93 0.6665358784817066 1107 94-95 0.6614205716089998 1107 96-97 0.7894986148196956 1107 98-99 0.6445099105557155 1107 100-101 0.4251638024866793 1108 1 0.08928291311375247 1108 2 0.03415367347722764 1108 3 -0.05349090984597993 1108 4 0.13720455458894776 1108 5 0.1120548346624588 1108 6 0.11524110537786214 1108 7 0.07021055798482934 1108 8 0.0403887931360245 1108 9 0.05686977447462738 1108 10-11 0.12407583296587177 1108 12-13 0.13878041968826693 1108 14-15 0.1564685379128079 1108 16-17 0.22767570437968487 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-1.4900180239001912 2316 90-91 -1.4406748295364125 2316 92-93 -1.6662619591355927 2316 94-95 -1.6673129908301938 2316 96-97 -1.3625448996014171 2316 98-99 -1.6761009860955731 2316 100-101 -1.6699680903043301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 9.0 4 26.0 5 96.0 6 238.0 7 540.0 8 1234.0 9 2249.0 10 3861.0 11 5978.0 12 8616.0 13 11841.0 14 14998.0 15 20279.0 16 26824.0 17 34373.0 18 29163.0 19 17114.0 20 14862.0 21 15389.0 22 16049.0 23 17649.0 24 20167.0 25 23855.0 26 28600.0 27 34504.0 28 41769.0 29 50535.0 30 60551.0 31 73287.0 32 89001.0 33 110905.0 34 145014.0 35 193622.0 36 255454.0 37 299085.0 38 145699.0 39 5499.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.637397481597006 20.0598970334141 15.857866077448207 20.44483940754069 2 16.05959609510216 22.246437847336 38.801348548858506 22.89261750870333 3 18.76624576125823 27.483045032920344 31.165142698808534 22.585566507012892 4 12.866196025480816 18.15038123915232 36.936463439910234 32.04695929545663 5 12.82319683243739 38.86113765285018 32.806890614193556 15.508774900518873 6 29.33052803801773 37.674174148584264 17.2681246945338 15.727173118864219 7 26.540388060965142 35.76042323527811 20.512375057794618 17.18681364596213 8 27.530194767174965 33.20531012275824 19.58232839877709 19.6821667112897 9 28.121802741265384 15.710789959652347 20.375592171488798 35.79181512759347 10-11 24.80663779314986 27.566355113466102 26.150729874632784 21.476277218751257 12-13 26.71763405190163 25.548052410715904 27.526703724859686 20.20760981252278 14-15 23.543204534946433 25.545908305986835 27.733554854170638 23.17733230489609 16-17 20.89116689326372 29.223102893386866 27.873004127126716 22.0127260862227 18-19 20.783683212690494 26.041123480671256 32.69536896972489 20.479824336913353 20-21 22.269710628291953 24.854756123608276 32.96842885365987 19.907104394439898 22-23 21.803254218688707 25.157285953794613 32.890639152608955 20.148820674907725 24-25 21.18770388282521 25.498074779807922 33.04406920565904 20.270152131707825 26-27 21.046818173071372 25.657628879866067 32.93700122880179 20.358551718260763 28-29 20.76284686133903 25.52436547400088 33.10984193235992 20.602945732300164 30-31 20.936058360772183 25.371860796649393 33.1380955339319 20.55398530864652 32-33 20.781804821768414 25.694949378881386 32.806426898673365 20.716818900676827 34-35 21.21946231351099 25.60594323840081 32.61810031221464 20.556494135873564 36-37 21.45813007224892 25.475352591831403 32.23086445513108 20.835652880788594 38-39 21.266886472485005 25.674778478237382 32.243028294798584 20.81530675447903 40-41 21.25568779858108 25.623290361756773 32.24814456746341 20.872877272198735 42-43 21.210473566762197 25.82887515317979 31.70875251038929 21.251898769668724 44-45 21.31212612173787 25.996610115446295 31.411491631669197 21.27977213114664 46-47 21.695232351431727 26.41304410571782 30.46205846108427 21.429665081766185 48-49 21.91916348568158 26.782345848903805 29.539837955314287 21.758652710100332 50-51 21.9019086767807 26.967595297074954 29.26589164271119 21.864604383433157 52-53 22.03169316651832 27.15775278032658 28.949152281464873 21.861401771690232 54-55 21.758021601689606 27.141263152628355 28.9507778252122 22.149937420469843 56-57 21.910080700472516 27.444153332272244 28.617178696966523 22.028587270288718 58-59 22.13546212305927 26.952177824789374 28.821951728386342 22.09040832376501 60-61 22.107654406780313 27.031749182066434 29.250107069442024 21.61048934171123 62-63 22.072053497745543 26.949274896184 29.35396293138 21.62470867469045 64-65 21.90083837312896 27.074347442561997 29.252759643737463 21.77205454057158 66-67 21.884530147083485 27.376863696115066 28.83073771550812 21.90786844129333 68-69 21.939938447464282 27.155025965757968 29.09107752122947 21.813958065548285 70-71 22.00220130869011 27.184787879387635 28.858557669999417 21.954453141922837 72-73 21.941090997454012 27.434204847765812 28.45474372175898 22.169960433021192 74-75 21.779244565723836 27.767758208693465 28.28632932193219 22.166667903650506 76-77 21.697707622182357 27.63253453520483 28.466673740379704 22.203084102233113 78-79 21.687191779692235 27.632703102407486 28.24760433446405 22.432500783436236 80-81 21.620756832596673 28.010739401822903 28.125203590393035 22.243300175187386 82-83 21.685207458470504 27.806651592107162 28.05721001518911 22.450930934233224 84-85 21.636407171669383 27.626552847750062 27.827427547517026 22.90961243306353 86-87 21.64511339623518 27.534856205695252 27.89120470974902 22.92882568832055 88-89 21.670997032334252 27.53576537518046 27.887371642134035 22.90586595035125 90-91 21.627302864871105 27.59100130296383 27.95192778171782 22.829768050447242 92-93 21.445928524108886 27.50516098706111 27.843985628953206 23.204924859876797 94-95 21.42931558234038 27.38629988171686 27.92936327493945 23.255021261003314 96-97 21.529790208713713 27.392004961232125 27.75469609979202 23.32350873026214 98-99 21.215700611904932 27.360754517815618 27.71225624137533 23.71128862890412 100-101 21.209569770459215 27.56218470578404 27.700589795352887 23.527655728403857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2918.0 1 2034.0 2 1279.0 3 1409.5 4 1216.0 5 1164.5 6 2009.5 7 4290.0 8 6838.5 9 7662.0 10 6285.0 11 4759.5 12 4554.5 13 4800.0 14 5121.5 15 5384.0 16 5642.0 17 5942.5 18 6189.0 19 6432.5 20 6840.5 21 7480.0 22 8367.0 23 9623.5 24 10933.0 25 12302.0 26 14303.5 27 16173.0 28 17632.0 29 19718.5 30 22488.5 31 25657.5 32 29332.5 33 32854.0 34 35911.0 35 37821.0 36 41392.5 37 46388.5 38 48453.0 39 48975.5 40 49587.0 41 50508.0 42 51503.0 43 53891.5 44 57179.0 45 58846.5 46 58332.5 47 58593.5 48 61886.0 49 61696.5 50 57954.0 51 54984.0 52 53136.5 53 53809.0 54 54724.5 55 53134.5 56 50717.0 57 47413.5 58 44099.0 59 41021.0 60 36569.5 61 31323.5 62 26245.5 63 20595.5 64 15276.5 65 11604.5 66 8233.5 67 6239.5 68 4349.0 69 2515.0 70 1724.0 71 1205.0 72 852.5 73 650.5 74 488.0 75 381.5 76 277.5 77 138.5 78 61.0 79 28.0 80 16.5 81 11.0 82 5.5 83 5.0 84 4.0 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02677382059121214 2 0.005332773300508372 3 2.7488522167568936E-4 4 2.1990817734055145E-4 5 1.649311330054136E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011215317044368124 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.473966995081204E-4 20-21 9.620982758649126E-4 22-23 0.002226570295573083 24-25 0.010335684335005918 26-27 0.005277796256173235 28-29 2.473966995081204E-4 30-31 0.0026388981280866175 32-33 0.004700537290654287 34-35 0.0 36-37 0.008768838571454489 38-39 0.011792576009887071 40-41 0.0014019146305460156 42-43 0.0 44-45 0.0 46-47 2.473966995081204E-4 48-49 0.003298622660108272 50-51 0.006349848620708423 52-53 0.0 54-55 0.0030512259606001514 56-57 0.0029962489162650135 58-59 2.473966995081204E-4 60-61 0.0015393572413838603 62-63 0.005387750344843511 64-65 8.24655665027068E-5 66-67 0.0023090358620757906 68-69 3.8483931034596507E-4 70-71 0.0012644720197081709 72-73 0.0 74-75 2.748852216756893E-5 76-77 0.0 78-79 0.0017042883743892737 80-81 0.0017317768965568426 82-83 0.011187828522200554 84-85 0.00799915995076256 86-87 0.004150766847302909 88-89 1.649311330054136E-4 90-91 6.047474876865165E-4 92-93 3.298622660108272E-4 94-95 2.748852216756893E-5 96-97 0.002089127684735239 98-99 0.0017042883743892737 100-101 2.7488522167568936E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1818941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.92607013582551 #Duplication Level Percentage of deduplicated Percentage of total 1 67.6211477694131 27.674678362760215 2 15.313707007918259 12.534596940910909 3 6.280869748206825 7.711539474872918 4 3.319130741210398 5.433559100190051 5 1.9023924242452737 3.892872289026259 6 1.1895039294900307 2.9209032747089423 7 0.807894807652219 2.314477169224079 8 0.5669088210564037 1.8561080136938028 9 0.42843033502922645 1.5780572945749207 >10 2.2847140959702896 16.864399576840682 >50 0.1784587081106498 5.053431040596211 >100 0.10050960014780705 7.208938538169353 >500 0.0040417094997389985 1.0227931212464438 >1k 0.0018861310998781996 1.3698260002289302 >5k 0.0 0.0 >10k+ 4.0417094997389987E-4 2.563819802956248 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19822 1.0897549728111027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15792 0.8681974841404972 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10885 0.5984251275879756 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3453 0.18983573408923105 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3236 0.17790571546850614 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2877 0.15816895655219163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2808 0.15437554049306712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.497704433513786E-5 0.0 8 0.0 0.0 0.0 5.497704433513786E-5 0.0 9 0.0 0.0 0.0 1.0995408867027572E-4 0.0 10-11 0.0 0.0 0.0 1.3744261083784465E-4 0.0 12-13 0.0 0.0 0.0 1.649311330054136E-4 0.0 14-15 0.0 0.0 0.0 2.1990817734055145E-4 0.0 16-17 0.0 0.0 0.0 2.748852216756893E-4 0.0 18-19 0.0 0.0 0.0 3.298622660108272E-4 0.0 20-21 0.0 0.0 0.0 4.947933990162407E-4 0.0 22-23 0.0 0.0 0.0 9.071212315297747E-4 0.0 24-25 0.0 0.0 0.0 0.0018417309852271184 0.0 26-27 0.0 0.0 0.0 0.002803829261092031 0.0 28-29 0.0 0.0 0.0 0.005717612610854338 0.0 30-31 0.0 0.0 0.0 0.011077874433530279 0.0 32-33 0.0 0.0 0.0 0.020423971970503718 0.0 34-35 0.0 0.0 0.0 0.029715092463142016 0.0 36-37 0.0 0.0 0.0 0.039088678522283024 0.0 38-39 0.0 0.0 0.0 0.051211116798180925 0.0 40-41 0.0 0.0 0.0 0.07133271502484137 0.0 42-43 0.0 0.0 0.0 0.0982164897047238 0.0 44-45 0.0 0.0 0.0 0.12408318906440616 0.0 46-47 0.0 0.0 0.0 0.15531015024676448 0.0 48-49 0.0 0.0 0.0 0.19000066522223646 0.0 50-51 0.0 0.0 0.0 0.23247043197113043 0.0 52-53 0.0 0.0 0.0 0.29844288517329587 0.0 54-55 0.0 0.0 0.0 0.3799738419223053 0.0 56-57 0.0 0.0 0.0 0.4553748582279469 0.0 58-59 0.0 0.0 0.0 0.5551581936962222 0.0 60-61 0.0 0.0 0.0 0.6726441374404117 0.0 62-63 0.0 0.0 0.0 0.7728672892633681 0.0 64-65 0.0 0.0 0.0 0.8732828607414974 0.0 66-67 0.0 0.0 0.0 1.022078231234548 0.0 68-69 0.0 0.0 0.0 1.1840680923680318 0.0 70-71 0.0 0.0 0.0 1.3140613136984651 0.0 72-73 0.0 0.0 0.0 1.493643829019193 0.0 74-75 0.0 0.0 0.0 1.6932104999557436 0.0 76-77 0.0 0.0 0.0 1.9028104814834566 0.0 78-79 0.0 0.0 0.0 2.1215091638486347 0.0 80-81 0.0 0.0 0.0 2.375310689021799 0.0 82-83 0.0 0.0 0.0 2.6641875684807808 0.0 84-85 0.0 0.0 0.0 2.957655031141747 0.0 86-87 0.0 0.0 0.0 3.2781162225712652 0.0 88-89 0.0 0.0 0.0 3.642366629813721 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8890 0.0 53.49897 1 GTATCAA 15835 0.0 41.737095 1 TATCAAC 21360 0.0 30.997835 2 ATCAACG 21410 0.0 30.923744 3 TCAACGC 21615 0.0 30.608484 4 CAACGCA 22010 0.0 30.046682 5 AACGCAG 22480 0.0 29.397346 6 ACGCAGA 25790 0.0 25.476994 7 CGCAGAG 25795 0.0 25.361547 8 GCAGAGT 26715 0.0 24.043564 9 GTACATG 14270 0.0 23.73989 1 GAGTACT 15445 0.0 23.477678 12-13 TAAGGTG 630 0.0 23.377522 5 GTAAGGT 590 0.0 23.344896 4 TACATGG 14730 0.0 22.765219 2 GTGGTAT 3950 0.0 22.61382 1 AGTACTT 15785 0.0 22.310053 12-13 TGGTATC 4010 0.0 22.149662 2 GTACTTT 16330 0.0 22.147146 14-15 GGACCGT 175 4.044625E-6 21.718472 6 >>END_MODULE