FastQCFastQC Report
Thu 26 May 2016
SRR937096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833214
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202801.1062538252489889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144160.7863784588160465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95650.5217612346403638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32420.1768478748253068No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.16670175986000543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.1262263980091795No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.12333530073412051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83900.049.2902531
GTATCAA162100.044.9827581
TATCAAC206200.035.2700842
ATCAACG208000.034.89633
TCAACGC208050.034.8431784
CAACGCA211000.034.2424555
AACGCAG217250.033.273776
ACGCAGA243100.029.4977877
CGCAGAG243500.029.1761118
GCAGAGT251200.027.428999
GAGTACT133350.025.08792712-13
GTACATG148600.025.0464781
AGTACTT136950.023.80385212-13
TACATGG152600.023.7669662
GTACTTT144500.023.08567414-15
ACATGGG153450.022.4891723
AGAGTAC225150.022.39170310-11
CAGAGTA242850.021.83583310-11
CGCATAC1100.0028661121.6063331
ATGGGAG38500.021.3594045
Produced by FastQC (version 0.11.5)