FastQCFastQC Report
Thu 26 May 2016
SRR937096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833214
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201501.099162454574316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160770.8769843564362917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109320.5963297247348099No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.19283073334591597No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.18797587188402445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29770.16239238845001183No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29190.1592285461490039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91650.050.6961481
GTATCAA161800.041.0484921
TATCAAC215350.030.8573382
ATCAACG217350.030.4414423
TCAACGC219300.030.2140794
CAACGCA223300.029.6303085
AACGCAG229000.028.9390056
ACGCAGA259550.025.4778697
CGCAGAG260100.025.2961438
GCAGAGT269450.023.9070729
GAGTACT155350.023.68173812-13
GTACATG145350.023.5007881
AGTACTT158700.023.19680612-13
TACATGG150300.022.501252
GTACTTT165200.022.18164414-15
AGAGTAC246950.021.2664310-11
ACATGGG149750.021.1876813
TACTTTT173950.021.03856514-15
GGTATAG3201.8189894E-1120.7848381
CAGAGTA264100.020.3980910-11
Produced by FastQC (version 0.11.5)