##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1833214 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69149264624861 31.0 31.0 34.0 30.0 34.0 2 31.803018087359142 31.0 31.0 34.0 30.0 34.0 3 31.89682164766361 31.0 31.0 34.0 30.0 34.0 4 35.69577092472564 37.0 35.0 37.0 33.0 37.0 5 35.49906612103115 37.0 35.0 37.0 33.0 37.0 6 35.60286687751675 37.0 35.0 37.0 33.0 37.0 7 35.53873306662506 37.0 35.0 37.0 33.0 37.0 8 35.49005244341359 37.0 35.0 37.0 33.0 37.0 9 37.06549753602143 39.0 37.0 39.0 33.0 39.0 10-11 36.96912662678771 39.0 37.0 39.0 33.0 39.0 12-13 36.823694069541254 39.0 37.0 39.0 32.0 39.0 14-15 37.80432508152349 39.5 37.0 41.0 32.5 41.0 16-17 37.818299445673006 39.5 37.0 41.0 32.5 41.0 18-19 38.02073107667735 40.0 37.0 41.0 33.0 41.0 20-21 38.05119751431093 40.0 37.0 41.0 33.0 41.0 22-23 37.93171664628352 40.0 37.0 41.0 33.0 41.0 24-25 37.80717390331952 40.0 37.0 41.0 32.5 41.0 26-27 37.54107430992781 39.0 36.5 41.0 32.0 41.0 28-29 37.333372154042024 39.0 36.0 41.0 32.0 41.0 30-31 37.162372750808146 39.0 36.0 41.0 31.5 41.0 32-33 36.901101562610805 39.0 35.0 41.0 31.0 41.0 34-35 36.689468059921 39.0 35.0 40.5 31.0 41.0 36-37 36.430798859271206 39.0 35.0 40.0 30.0 41.0 38-39 36.1906926850875 38.0 35.0 40.0 30.0 41.0 40-41 35.76689191769209 38.0 35.0 40.0 29.0 41.0 42-43 35.41079328436287 38.0 35.0 40.0 27.5 41.0 44-45 34.978087119125206 38.0 34.0 40.0 26.0 41.0 46-47 34.35537749548062 38.0 33.5 40.0 23.5 41.0 48-49 33.77683292839788 38.0 33.0 40.0 20.5 41.0 50-51 33.38555345966155 38.0 33.0 40.0 18.5 41.0 52-53 33.058457168666614 37.0 32.5 40.0 16.0 41.0 54-55 32.60749099668669 37.0 32.0 40.0 12.5 41.0 56-57 32.36961096740479 36.5 31.0 39.5 10.0 41.0 58-59 32.00538971445778 36.0 31.0 39.0 8.0 40.0 60-61 31.580554152433923 35.5 31.0 39.0 7.0 40.0 62-63 31.16992969724211 35.0 30.0 38.5 4.0 40.0 64-65 30.814881405007817 35.0 30.0 38.0 2.0 40.0 66-67 30.453936638057534 35.0 29.0 38.0 2.0 40.0 68-69 29.984008413638563 34.0 29.0 37.0 2.0 39.5 70-71 29.526696283139884 34.0 28.0 37.0 2.0 39.0 72-73 29.11015680657032 34.0 28.0 36.0 2.0 39.0 74-75 28.612059203126314 34.0 27.0 36.0 2.0 38.5 76-77 27.26400491159243 31.5 25.5 34.5 2.0 36.0 78-79 27.942165235482598 33.0 26.0 35.0 2.0 37.0 80-81 27.79738399335811 33.0 26.0 35.0 2.0 36.5 82-83 27.42763338050004 33.0 26.0 35.0 2.0 36.0 84-85 26.958758770116308 33.0 25.5 35.0 2.0 36.0 86-87 26.52450041293597 32.0 25.0 35.0 2.0 35.0 88-89 26.083841002741636 32.0 24.0 34.0 2.0 35.0 90-91 25.540331897967178 32.0 22.0 34.0 2.0 35.0 92-93 24.95640798073766 31.0 19.5 34.0 2.0 35.0 94-95 24.27960920001702 31.0 14.0 34.0 2.0 35.0 96-97 23.369516052135758 31.0 2.0 34.0 2.0 35.0 98-99 22.345474123588406 30.0 2.0 34.0 2.0 35.0 100-101 19.299448127714495 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20773459263298477 1101 2 0.3556982729781275 1101 3 0.3294335836539375 1101 4 0.18939397263616797 1101 5 -0.010493080370245877 1101 6 0.23190547824685126 1101 7 0.1452691493307583 1101 8 0.24360855563886474 1101 9 0.2760721168375042 1101 10-11 0.3320939681312609 1101 12-13 0.32540753914174303 1101 14-15 0.4818679942792059 1101 16-17 0.47679542646984885 1101 18-19 0.2948544022904258 1101 20-21 0.14970954408402548 1101 22-23 0.2639772278135055 1101 24-25 0.2857370631908438 1101 26-27 0.27467564936169 1101 28-29 0.21457461455145932 1101 30-31 0.4324288464892305 1101 32-33 0.42113018707387084 1101 34-35 0.2924223850938077 1101 36-37 0.5275022760413748 1101 38-39 0.49316357120807197 1101 40-41 0.6974845739081772 1101 42-43 0.800020975140832 1101 44-45 0.7809159199358078 1101 46-47 0.3400633806960798 1101 48-49 0.20879115081785926 1101 50-51 0.34083698284473485 1101 52-53 0.5923675493935363 1101 54-55 0.42636946667764875 1101 56-57 0.5558757898386375 1101 58-59 0.3412526401954139 1101 60-61 0.46791195326091284 1101 62-63 0.4915608143899277 1101 64-65 0.5064003031172852 1101 66-67 0.3571731656668078 1101 68-69 0.2523258378106483 1101 70-71 0.1694303865704505 1101 72-73 0.3410684614072146 1101 74-75 0.20519046612047376 1101 76-77 0.3761145656647855 1101 78-79 0.399518697476406 1101 80-81 0.5087084649460003 1101 82-83 0.7010576027493016 1101 84-85 0.6357130734845704 1101 86-87 0.5668128481644459 1101 88-89 0.53022637644996 1101 90-91 0.8744964530924406 1101 92-93 0.5456722121881121 1101 94-95 0.4915465145396922 1101 96-97 0.27623395266094164 1101 98-99 0.49707149230247083 1101 100-101 0.8604750795565295 1102 1 -0.07827855048929422 1102 2 0.062088513808248535 1102 3 0.11022148916167396 1102 4 0.1693487754575571 1102 5 -0.03508399736679735 1102 6 0.13782462140726182 1102 7 0.11371808639967185 1102 8 0.2528528289968506 1102 9 0.19272371199975424 1102 10-11 0.329118188664836 1102 12-13 0.31673256417585094 1102 14-15 0.3488048597953508 1102 16-17 0.22736848070011462 1102 18-19 0.3290001715698594 1102 20-21 0.20500949398692114 1102 22-23 0.06972498918950265 1102 24-25 0.15469341399537484 1102 26-27 0.2072897011656707 1102 28-29 0.022913759803593337 1102 30-31 0.2165828497215614 1102 32-33 0.26580258842389526 1102 34-35 0.4679327023812263 1102 36-37 0.29440398113411703 1102 38-39 0.47641181073115746 1102 40-41 0.5865379813490108 1102 42-43 0.3983333614906144 1102 44-45 0.6002211014323535 1102 46-47 0.46956742515595806 1102 48-49 0.6349692850214694 1102 50-51 0.470057894889365 1102 52-53 0.6005538320324959 1102 54-55 0.9148202643310057 1102 56-57 0.6050129871397445 1102 58-59 0.530411384451142 1102 60-61 0.6707695393895072 1102 62-63 0.4953517551927149 1102 64-65 0.6282771070030115 1102 66-67 0.6296667359607859 1102 68-69 0.60111882698979 1102 70-71 0.4611815836716353 1102 72-73 0.3714159226628553 1102 74-75 0.4754738701117134 1102 76-77 0.3496608973954345 1102 78-79 -0.05000081525848543 1102 80-81 0.27829833897518697 1102 82-83 0.4749840253018931 1102 84-85 0.46441016166991744 1102 86-87 0.38851439886335015 1102 88-89 0.2827050851812416 1102 90-91 0.17179097840939406 1102 92-93 0.19190461536404868 1102 94-95 0.15975129323703996 1102 96-97 0.7040288307377658 1102 98-99 0.5686197759133194 1102 100-101 0.7464605450406658 1103 1 0.1458388151483092 1103 2 0.26160154685792136 1103 3 0.26256345491054844 1103 4 0.1721039974447791 1103 5 0.05716858467290109 1103 6 0.11563997012062543 1103 7 0.16610085822550502 1103 8 0.1958937151706266 1103 9 0.17892757786140834 1103 10-11 0.1599886353236073 1103 12-13 0.2719627775580804 1103 14-15 0.3002881119855658 1103 16-17 0.16499011419876553 1103 18-19 0.18578383008902222 1103 20-21 0.1281392876185805 1103 22-23 0.16621810194632758 1103 24-25 0.249170035468687 1103 26-27 0.24838802324575937 1103 28-29 0.12501419037806016 1103 30-31 0.371456253467926 1103 32-33 0.2708129685373706 1103 34-35 0.4454122398113398 1103 36-37 0.36916001058376935 1103 38-39 0.23386487291875824 1103 40-41 0.336350186803827 1103 42-43 -0.03941301737595637 1103 44-45 0.09878720619465753 1103 46-47 0.1145228956597748 1103 48-49 0.10257689075467624 1103 50-51 -0.06942523174369342 1103 52-53 0.03645771369703965 1103 54-55 0.4395653075291577 1103 56-57 0.19387985541946762 1103 58-59 0.10973239081342712 1103 60-61 0.06237122661796235 1103 62-63 0.3452443821732203 1103 64-65 0.2351892992526743 1103 66-67 0.2384390142387467 1103 68-69 0.07933465974765497 1103 70-71 -0.06739971783656884 1103 72-73 0.016860903731867438 1103 74-75 -0.051141000618528665 1103 76-77 -0.038716622677476664 1103 78-79 -0.059414625680165756 1103 80-81 -0.06881335927198862 1103 82-83 0.09940792359193651 1103 84-85 -0.040085271729587646 1103 86-87 -0.06791429008177374 1103 88-89 0.17270510682902795 1103 90-91 -0.004790554730121244 1103 92-93 0.16071692039867358 1103 94-95 0.3721052518594625 1103 96-97 0.5716371941434808 1103 98-99 0.34447562114030816 1103 100-101 0.7001865078800762 1104 1 0.19446020569382227 1104 2 0.32712930159233267 1104 3 0.22640378999889776 1104 4 0.14746796352168445 1104 5 0.026855862048684287 1104 6 0.1947673511863357 1104 7 0.210888012474733 1104 8 0.24410110966832832 1104 9 0.07270398547412782 1104 10-11 0.27225884528733957 1104 12-13 0.30926452758711065 1104 14-15 0.3343282374344767 1104 16-17 0.2744727159037055 1104 18-19 0.41847680769086537 1104 20-21 0.43795766287108506 1104 22-23 0.34616659696739305 1104 24-25 0.39632340073611516 1104 26-27 0.3390296203551699 1104 28-29 0.3216961807392451 1104 30-31 0.4067459513214189 1104 32-33 0.3899052213976759 1104 34-35 0.3427515502398464 1104 36-37 0.29965457240700033 1104 38-39 0.23196292329289747 1104 40-41 0.2500397258585849 1104 42-43 0.3668431611816132 1104 44-45 0.48640114010383684 1104 46-47 0.22511358407007975 1104 48-49 0.1722988726633332 1104 50-51 0.21685591210934518 1104 52-53 0.2413252140396409 1104 54-55 0.5358631942924816 1104 56-57 0.42596243076256 1104 58-59 0.24278609156672104 1104 60-61 0.27137633370250214 1104 62-63 0.2366487467324383 1104 64-65 0.24192713069796312 1104 66-67 0.06710519376919066 1104 68-69 0.06409787911482212 1104 70-71 0.23301299673264353 1104 72-73 0.3765897323976546 1104 74-75 0.22802623704711067 1104 76-77 0.41753866669085227 1104 78-79 0.4018249616523413 1104 80-81 0.23008930275136308 1104 82-83 0.5185008564204878 1104 84-85 0.5938913551467309 1104 86-87 0.5407860866206278 1104 88-89 0.6169042977714341 1104 90-91 0.6455402236661385 1104 92-93 0.40896822515311726 1104 94-95 0.5944221756412986 1104 96-97 0.6907805753849381 1104 98-99 0.5936179458157262 1104 100-101 1.327009607342692 1105 1 0.10576680703471908 1105 2 0.1722283217057914 1105 3 0.06499043259817583 1105 4 0.010127324016785622 1105 5 0.06043734735038697 1105 6 0.17470082204760473 1105 7 0.2309101888543097 1105 8 0.14835923241200533 1105 9 0.16101986995065687 1105 10-11 0.18821023775767998 1105 12-13 0.20626298039783109 1105 14-15 0.35051377121477856 1105 16-17 0.05650482008110913 1105 18-19 0.0014070552411453718 1105 20-21 0.17266717808510634 1105 22-23 0.14497925813294188 1105 24-25 0.10960563384596611 1105 26-27 0.15746205975691652 1105 28-29 0.12091936279185234 1105 30-31 0.2377752189851634 1105 32-33 0.23972574744203 1105 34-35 0.3277403331176245 1105 36-37 0.27924469618214687 1105 38-39 0.2227173069958326 1105 40-41 0.29647848294986545 1105 42-43 0.4086103091960567 1105 44-45 0.26940333195530286 1105 46-47 0.036186688763223174 1105 48-49 0.04365498406096435 1105 50-51 0.036040285497691116 1105 52-53 0.346083981445517 1105 54-55 0.43404705195106175 1105 56-57 0.26377792844177606 1105 58-59 0.19253918594527164 1105 60-61 0.12610144974169302 1105 62-63 0.27002277973914346 1105 64-65 0.06552550614922481 1105 66-67 0.1700231927377338 1105 68-69 0.33321701268883075 1105 70-71 0.15619865944537992 1105 72-73 0.036870031520912505 1105 74-75 0.05055524684597401 1105 76-77 -0.030012261622708536 1105 78-79 0.1386365913583738 1105 80-81 0.03397416608297732 1105 82-83 -0.040817735637272534 1105 84-85 -0.2174316206139686 1105 86-87 0.002107515192058429 1105 88-89 0.3297208269101617 1105 90-91 0.1883646176836713 1105 92-93 0.17736752891794438 1105 94-95 0.4214911544963762 1105 96-97 0.5045154902895241 1105 98-99 0.6452190288482171 1105 100-101 0.8760125727887988 1106 1 0.02811399202131426 1106 2 0.17563582840820402 1106 3 0.1504153655740481 1106 4 0.1997372435878333 1106 5 0.03415986745762467 1106 6 0.18478929389747378 1106 7 0.17632305748701782 1106 8 0.25480561310905614 1106 9 0.2545547224975806 1106 10-11 0.2739790045137127 1106 12-13 0.325405071550648 1106 14-15 0.47253454869467504 1106 16-17 0.352425061367974 1106 18-19 0.38448413298914375 1106 20-21 0.2468970708465008 1106 22-23 0.22357376215438762 1106 24-25 0.2890164917548148 1106 26-27 0.44088431176763976 1106 28-29 0.29449510005726864 1106 30-31 0.39739921362323116 1106 32-33 0.15604210132943308 1106 34-35 0.19328255831869257 1106 36-37 0.23721587580681103 1106 38-39 0.2401187815266681 1106 40-41 0.4669523435396741 1106 42-43 0.45041862018801027 1106 44-45 0.20509032927433424 1106 46-47 0.41260693005008164 1106 48-49 0.16398608325667396 1106 50-51 0.28673867691860266 1106 52-53 0.27720797608358794 1106 54-55 0.6709116632111218 1106 56-57 0.4190869096750163 1106 58-59 0.5137221618165881 1106 60-61 0.14544729424571656 1106 62-63 0.3062540129831106 1106 64-65 0.17463877692670593 1106 66-67 0.4812671605661514 1106 68-69 0.3630990502223419 1106 70-71 0.38890911520945437 1106 72-73 0.16479362401420872 1106 74-75 0.15890310207385738 1106 76-77 0.49869752390007704 1106 78-79 0.3655957066398763 1106 80-81 0.3363086164851232 1106 82-83 0.34264406060937347 1106 84-85 0.3921843311286608 1106 86-87 0.37498754200170836 1106 88-89 0.5941745802613667 1106 90-91 0.44907373296517505 1106 92-93 0.6457757058616416 1106 94-95 0.7378256048985214 1106 96-97 0.8832715224611078 1106 98-99 0.8388343103495579 1106 100-101 1.256931473593088 1107 1 0.2140036491399826 1107 2 0.3753617245197596 1107 3 0.4511240959645093 1107 4 0.2707701067161068 1107 5 0.1959222353128638 1107 6 0.2285252097495345 1107 7 0.18209153330454342 1107 8 0.18030998801388165 1107 9 0.3112363889709826 1107 10-11 0.3185256364028106 1107 12-13 0.30507486158595754 1107 14-15 0.5234899306728309 1107 16-17 0.4917496090336968 1107 18-19 0.31941812871691866 1107 20-21 0.28885438496369176 1107 22-23 0.19862555828927242 1107 24-25 0.40374880737384444 1107 26-27 0.5581131801321177 1107 28-29 0.42599036331295537 1107 30-31 0.3053725352957457 1107 32-33 0.32521714921379186 1107 34-35 0.4987365551082448 1107 36-37 0.3802711422478069 1107 38-39 0.42687168427567457 1107 40-41 0.5996365079628987 1107 42-43 0.4474761247249788 1107 44-45 0.28708636999594717 1107 46-47 0.2158710295657187 1107 48-49 0.4577511278858708 1107 50-51 0.28504054605005535 1107 52-53 0.44562944783425706 1107 54-55 0.5039019242804059 1107 56-57 0.3432936918602252 1107 58-59 0.23188454811306514 1107 60-61 0.2672416269173077 1107 62-63 0.28604297254789657 1107 64-65 0.44989119140196365 1107 66-67 0.4706391385428397 1107 68-69 0.20900544416355515 1107 70-71 0.36578855001338084 1107 72-73 0.526576388998766 1107 74-75 0.4973719482529546 1107 76-77 0.14498838975821826 1107 78-79 0.575096857121796 1107 80-81 0.649092587135609 1107 82-83 0.7983660840604863 1107 84-85 0.6666018603657093 1107 86-87 0.8466373484385414 1107 88-89 0.8796550374364784 1107 90-91 0.7238171971521439 1107 92-93 0.8733283157861038 1107 94-95 1.1448774233864256 1107 96-97 1.1170365950315784 1107 98-99 1.393919133069165 1107 100-101 1.7039168197924468 1108 1 -0.03182278134300631 1108 2 0.04039054930869668 1108 3 -0.013838865222648877 1108 4 0.025101900287971546 1108 5 -0.10765345168109519 1108 6 -0.040304020567404564 1108 7 -0.02003654238055219 1108 8 -0.005237619888234235 1108 9 0.08757798133080286 1108 10-11 0.0259384459901284 1108 12-13 0.03129930001042425 1108 14-15 0.04776256056102568 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-1.5519282238488081 2316 62-63 -1.6355165377682752 2316 64-65 -1.682254167441279 2316 66-67 -1.8680005461346703 2316 68-69 -1.9454951534832787 2316 70-71 -1.7029437737890412 2316 72-73 -2.1068406509716695 2316 74-75 -1.6868371863884448 2316 76-77 -1.7880130034624742 2316 78-79 -1.7865956044873208 2316 80-81 -1.8775043205639044 2316 82-83 -1.8390187742129385 2316 84-85 -1.655901205183703 2316 86-87 -1.6709985678939958 2316 88-89 -2.1934357012797463 2316 90-91 -2.2900611983400694 2316 92-93 -2.083845379093926 2316 94-95 -2.405067450844868 2316 96-97 -2.3579652218766434 2316 98-99 -2.572654858392138 2316 100-101 -2.6962136883387977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 38.0 5 106.0 6 264.0 7 564.0 8 1352.0 9 2463.0 10 4132.0 11 6439.0 12 9046.0 13 12510.0 14 15816.0 15 21247.0 16 27274.0 17 34742.0 18 29223.0 19 17749.0 20 15315.0 21 15936.0 22 16768.0 23 18434.0 24 21281.0 25 25102.0 26 29989.0 27 36314.0 28 43958.0 29 52522.0 30 63049.0 31 76305.0 32 91972.0 33 114855.0 34 148528.0 35 196601.0 36 257782.0 37 291183.0 38 130281.0 39 4060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.599202109956494 20.125117713879163 15.851408256285076 20.42427191987927 2 16.045789783761375 22.296471666412096 38.834744375831896 22.822994173994633 3 18.74907948352889 27.49695888632508 31.228937219412945 22.525024410733085 4 12.852563857792926 18.206876011202183 37.010681786196265 31.92987834480863 5 12.818185339158584 38.81873411562164 32.79883087702225 15.56424966819753 6 29.357401809063212 37.65266902827493 17.289470841920256 15.700458320741607 7 26.46895496855785 35.883271292728374 20.464671766651684 17.18310197206209 8 27.519536726208727 33.14784853268631 19.610094620704402 19.722520120400564 9 28.098589743799547 15.68252025269295 20.448360337833083 35.770529665674424 10-11 24.78016750908514 27.59915099928323 26.15499336138607 21.46568813024557 12-13 26.704782104564607 25.630627202704904 27.517169540053533 20.14742115267696 14-15 23.51131063251552 25.483228591869416 27.82052565386249 23.184935121752577 16-17 20.80548152043351 29.233057351733077 27.90528001640834 22.056181111425072 18-19 20.75011632607084 26.01811904698264 32.80201548214088 20.42974914480564 20-21 22.244045092881603 24.795848120820818 33.06256730056939 19.89753948572819 22-23 21.7693913976993 25.155058084731262 32.95255686281355 20.122993654755877 24-25 21.133686432414635 25.43993510788396 33.181440178398766 20.244938281302634 26-27 21.030788931422112 25.61319436015836 33.01644794710909 20.339568761310435 28-29 20.709786587810584 25.511710859567327 33.1871691941962 20.59133335842589 30-31 20.927780390069024 25.43772849214549 33.155130824879144 20.47936029290634 32-33 20.777468120075913 25.7192995662792 32.860337103229476 20.642895210415407 34-35 21.15134948783939 25.622540521728503 32.70125582719748 20.524854163234625 36-37 21.433436114516695 25.477605202973518 32.28025578468047 20.80870289782932 38-39 21.197160259010303 25.658593347879183 32.34178252746986 20.802463865640654 40-41 21.205400855666433 25.61377946978793 32.25514962707104 20.925670047474597 42-43 21.200334494499824 25.854482891795506 31.764185741544633 21.180996872160044 44-45 21.301846966131 26.046894616531624 31.420053125812274 21.2312052915251 46-47 21.622560015011935 26.42577928963871 30.51481337633264 21.43684731901672 48-49 21.839347875951983 26.799910212117055 29.636165424628224 21.724576487302734 50-51 21.833891650015357 26.962577235626007 29.29509723220028 21.90843388215836 52-53 21.989484528040666 27.148240770869137 29.061067142845843 21.80120755824435 54-55 21.720513807989683 27.179014561311444 29.021407211596685 22.07906441910219 56-57 21.836357080554283 27.488570600983735 28.638329139417124 22.03674317904486 58-59 22.040016124712324 26.965744805153363 28.902237658403752 22.092001411730564 60-61 22.08585878146798 27.03689177180768 29.27601486602737 21.601234580696964 62-63 21.98202173886955 26.98997498382622 29.430770221043478 21.59723305626075 64-65 21.804930854950452 27.0967916140609 29.343286358393456 21.754991172595197 66-67 21.82697214481565 27.45315807460506 28.887415459246835 21.83245432133246 68-69 21.836633277202377 27.18772995827544 29.113938205762235 21.861698558759947 70-71 21.885474315604178 27.277998717546243 28.906867728068526 21.929659238781053 72-73 21.884547806605795 27.47177217654943 28.49519612875564 22.14848388808914 74-75 21.72034611159055 27.779862816569807 28.412293267494597 22.087497804345052 76-77 21.64772631191936 27.650554136976048 28.52745810493173 22.174261446172864 78-79 21.605593029278417 27.64232476303185 28.331743681372107 22.420338526317625 80-81 21.476561871395432 28.06293989534565 28.246446402489667 22.21405183076925 82-83 21.610021099578226 27.8159590925654 28.119251892307055 22.45476791554932 84-85 21.5390435480378 27.658439938227257 27.95282054974686 22.849695963988083 86-87 21.52498705821322 27.582343311646408 27.979024759315497 22.913644870824875 88-89 21.580621062309977 27.63842108091168 27.925574641242157 22.855383215536186 90-91 21.561594815594674 27.62400514949351 28.00896251888784 22.80543751602398 92-93 21.39911106941143 27.558430166909048 27.91842632665908 23.124032437020446 94-95 21.341016378884298 27.39641962149536 27.97417540996305 23.28838858965729 96-97 21.494792062999824 27.392093056365834 27.73501584514389 23.37809903549045 98-99 21.192844666665938 27.35049961651882 27.715461328404988 23.74119438841025 100-101 21.029925740697404 27.61272679962753 27.808698697502855 23.548648762172213 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3147.0 1 2204.0 2 1367.0 3 1465.5 4 1254.0 5 1229.0 6 2085.0 7 4371.5 8 6915.0 9 7842.5 10 6551.0 11 4910.5 12 4644.0 13 4852.0 14 5204.5 15 5473.5 16 5653.0 17 6000.5 18 6349.5 19 6676.5 20 7118.0 21 7718.5 22 8600.0 23 9838.0 24 11033.5 25 12508.5 26 14481.0 27 16317.5 28 17856.5 29 19930.5 30 22788.0 31 25987.5 32 29587.0 33 32956.0 34 36232.0 35 38439.0 36 42010.5 37 46939.5 38 49087.5 39 49352.0 40 49782.0 41 51184.5 42 52277.0 43 54476.5 44 57434.5 45 58999.5 46 58745.5 47 58889.5 48 62342.5 49 62165.5 50 58304.5 51 55327.5 52 53131.5 53 54022.0 54 55079.0 55 53349.5 56 50690.0 57 47132.5 58 44281.5 59 41286.0 60 36460.5 61 31286.0 62 26331.0 63 20743.5 64 15452.0 65 11660.5 66 8166.5 67 6174.5 68 4323.0 69 2471.0 70 1707.0 71 1208.5 72 874.0 73 658.0 74 465.5 75 381.5 76 279.0 77 129.0 78 65.5 79 32.0 80 13.0 81 9.5 82 6.5 83 5.0 84 3.0 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020728621972121095 2 0.0029456462802487874 3 2.1819602075916943E-4 4 0.0 5 0.0024547052335406557 6 0.0 7 3.272940311387541E-4 8 0.0 9 5.454900518979236E-5 10-11 0.0 12-13 0.005373077011194547 14-15 0.0027001757568947213 16-17 0.0 18-19 2.727450259489618E-4 20-21 0.0010909801037958472 22-23 0.0016364701556937707 24-25 9.546075908213662E-4 26-27 3.545685337336503E-4 28-29 5.454900518979236E-5 30-31 0.0 32-33 3.8184303632854644E-4 34-35 0.0 36-37 0.0017182936634784593 38-39 0.008700566327771879 40-41 0.0012546271193652242 42-43 0.0 44-45 0.0010091565960111586 46-47 3.272940311387541E-4 48-49 2.4547052335406556E-4 50-51 0.0013637251297448087 52-53 5.727645544928197E-4 54-55 0.0 56-57 1.6364701556937706E-4 58-59 1.6364701556937706E-4 60-61 0.0022365092127814862 62-63 0.0 64-65 1.363725129744809E-4 66-67 2.727450259489618E-5 68-69 5.454900518979236E-5 70-71 5.182155493030274E-4 72-73 2.727450259489618E-4 74-75 0.002618352249110033 76-77 1.6364701556937706E-4 78-79 1.9092151816427322E-4 80-81 0.0019910386894274206 82-83 1.0909801037958472E-4 84-85 0.002345607223161071 86-87 2.727450259489618E-4 88-89 0.0026456267517049295 90-91 0.0013091761245550165 92-93 0.0 94-95 0.0 96-97 1.363725129744809E-4 98-99 4.3639204151833886E-4 100-101 0.002345607223161071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1833214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.28945171632425 #Duplication Level Percentage of deduplicated Percentage of total 1 68.26478099751891 28.186153789225067 2 14.848066628937264 12.261370603125414 3 6.2343210151421316 7.722350896163299 4 3.1736045449542227 5.241455665023783 5 1.9385091734770814 4.00199904599668 6 1.2113061210479081 3.0008499359217375 7 0.8130341361998736 2.3498813597243657 8 0.5813782050845656 1.9203829862209947 9 0.4250394585050212 1.5794681579528111 >10 2.2289627251078237 16.625253621841967 >50 0.1757485977008123 5.001723735084504 >100 0.09912810500222684 7.145739315471447 >500 0.003858444529288586 0.9884551283605285 >1k 0.0018626973589669037 1.3842107413605422 >5k 0.0 0.0 >10k+ 3.9914943406433646E-4 2.5907050185268803 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20150 1.099162454574316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16077 0.8769843564362917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10932 0.5963297247348099 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3535 0.19283073334591597 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3446 0.18797587188402445 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2977 0.16239238845001183 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.1592285461490039 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.454900518979235E-5 0.0 14-15 0.0 0.0 0.0 5.454900518979235E-5 0.0 16-17 0.0 0.0 0.0 1.090980103795847E-4 0.0 18-19 0.0 0.0 0.0 1.090980103795847E-4 0.0 20-21 0.0 0.0 0.0 1.9092151816427325E-4 0.0 22-23 0.0 0.0 0.0 4.091175389234426E-4 0.0 24-25 0.0 0.0 0.0 0.0013364506271499126 0.0 26-27 0.0 0.0 0.0 0.002318332720566175 0.0 28-29 0.0 0.0 0.0 0.004936684969676208 0.0 30-31 0.0 0.0 0.0 0.01191895763396963 0.0 32-33 0.0 0.0 0.0 0.021928700086296528 0.0 34-35 0.0 0.0 0.0 0.030874736937422473 0.0 36-37 0.0 0.0 0.0 0.04093902839493916 0.0 38-39 0.0 0.0 0.0 0.05569453429877799 0.0 40-41 0.0 0.0 0.0 0.07871421448887037 0.0 42-43 0.0 0.0 0.0 0.10748881472648583 0.0 44-45 0.0 0.0 0.0 0.13533608187587481 0.0 46-47 0.0 0.0 0.0 0.16910191608835629 0.0 48-49 0.0 0.0 0.0 0.20425874993317747 0.0 50-51 0.0 0.0 0.0 0.24947987523551535 0.0 52-53 0.0 0.0 0.0 0.3190298568525006 0.0 54-55 0.0 0.0 0.0 0.4057627751042704 0.0 56-57 0.0 0.0 0.0 0.482595048914093 0.0 58-59 0.0 0.0 0.0 0.5886655895056442 0.0 60-61 0.0 0.0 0.0 0.7118099687216004 0.0 62-63 0.0 0.0 0.0 0.8211261751219443 0.0 64-65 0.0 0.0 0.0 0.9216599916867316 0.0 66-67 0.0 0.0 0.0 1.074424480720745 0.0 68-69 0.0 0.0 0.0 1.2419717501611922 0.0 70-71 0.0 0.0 0.0 1.384644673235094 0.0 72-73 0.0 0.0 0.0 1.5642745473250805 0.0 74-75 0.0 0.0 0.0 1.772460825631923 0.0 76-77 0.0 0.0 0.0 1.9846837303228102 0.0 78-79 0.0 0.0 0.0 2.211280297881207 0.0 80-81 0.0 0.0 0.0 2.4711790331079735 0.0 82-83 0.0 0.0 0.0 2.762088877785136 0.0 84-85 0.0 0.0 0.0 3.057989956437164 0.0 86-87 0.0 0.0 0.0 3.3837566154306042 0.0 88-89 0.0 0.0 0.0 3.7518805769539183 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9165 0.0 50.696148 1 GTATCAA 16180 0.0 41.048492 1 TATCAAC 21535 0.0 30.857338 2 ATCAACG 21735 0.0 30.441442 3 TCAACGC 21930 0.0 30.214079 4 CAACGCA 22330 0.0 29.630308 5 AACGCAG 22900 0.0 28.939005 6 ACGCAGA 25955 0.0 25.477869 7 CGCAGAG 26010 0.0 25.296143 8 GCAGAGT 26945 0.0 23.907072 9 GAGTACT 15535 0.0 23.681738 12-13 GTACATG 14535 0.0 23.500788 1 AGTACTT 15870 0.0 23.196806 12-13 TACATGG 15030 0.0 22.50125 2 GTACTTT 16520 0.0 22.181644 14-15 AGAGTAC 24695 0.0 21.26643 10-11 ACATGGG 14975 0.0 21.187681 3 TACTTTT 17395 0.0 21.038565 14-15 GGTATAG 320 1.8189894E-11 20.784838 1 CAGAGTA 26410 0.0 20.39809 10-11 >>END_MODULE