FastQCFastQC Report
Thu 26 May 2016
SRR937095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208302
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80750.36566556566991293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.2721548049134584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.16044001228092897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60650.038.087091
GTATCAA96700.037.110161
TATCAAC119850.029.8237342
ATCAACG120200.029.6557853
TCAACGC120500.029.6213954
CAACGCA122100.029.231255
AACGCAG125800.028.4067066
GTACATG98000.025.1717621
ACGCAGA140900.025.058877
CGCAGAG141500.024.7516758
TACATGG100400.024.1445242
ACATGGG102700.023.1856863
GCAGAGT148650.022.8258449
GAGTACT68400.022.7859412-13
GTACTTT74550.021.00206214-15
AGAGTAC123150.020.29576910-11
CATGGGA49000.019.9813024
AGTACTT73150.019.81229212-13
CATGGGG59750.019.64774
TATTCCG2702.287743E-819.362085
Produced by FastQC (version 0.11.5)