FastQCFastQC Report
Thu 26 May 2016
SRR937095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208302
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.4311457400301226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77940.35294085682121373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48630.2202144453068466No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.10043916094809495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73600.036.337551
GTATCAA114000.035.2942241
TATCAAC141550.028.2203272
ATCAACG142900.027.8207683
TCAACGC144450.027.55454
CAACGCA146400.027.1874855
AACGCAG148750.026.757366
ACGCAGA167650.023.542567
CGCAGAG168350.023.3882458
GAGTACT90750.022.94981612-13
GTACATG95700.022.5852321
TACATGG96050.021.9564192
GCAGAGT179950.021.511079
AGTACTT95350.021.29470812-13
GTACTTT98150.021.17160614-15
AGAGTAC144200.020.98120310-11
ACATGGG96200.020.4903283
CGTAACG600.00476735319.79041114-15
TACTTTT106800.018.92319114-15
GTATAGG6350.018.7021431
Produced by FastQC (version 0.11.5)