##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937095_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2208302 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.181464763424565 33.0 31.0 34.0 31.0 34.0 2 32.17042098408641 33.0 31.0 34.0 30.0 34.0 3 32.35632762185607 34.0 31.0 34.0 31.0 34.0 4 36.087860718325665 37.0 35.0 37.0 35.0 37.0 5 36.014329561808125 37.0 35.0 37.0 35.0 37.0 6 36.06038802663766 37.0 35.0 37.0 35.0 37.0 7 36.00080242647971 37.0 35.0 37.0 35.0 37.0 8 35.98158766328156 37.0 35.0 37.0 35.0 37.0 9 37.67417047124895 39.0 37.0 39.0 35.0 39.0 10-11 37.59985047335011 39.0 37.0 39.0 35.0 39.0 12-13 37.50425462640527 39.0 37.0 39.0 35.0 39.0 14-15 38.731277696619394 40.0 38.0 41.0 35.0 41.0 16-17 38.64103822756127 40.0 38.0 41.0 34.0 41.0 18-19 38.686963105589726 40.0 38.0 41.0 34.5 41.0 20-21 38.757613089151754 40.0 38.0 41.0 35.0 41.0 22-23 38.687424319680915 40.0 38.0 41.0 34.0 41.0 24-25 38.58114741552559 40.0 38.0 41.0 34.0 41.0 26-27 38.42195496811577 40.0 38.0 41.0 34.0 41.0 28-29 38.246297834263615 40.0 38.0 41.0 34.0 41.0 30-31 38.11750566725022 40.0 38.0 41.0 33.5 41.0 32-33 38.11833866020137 40.0 37.0 41.0 34.0 41.0 34-35 38.03738890785771 40.0 37.0 41.0 33.0 41.0 36-37 37.88955043286652 40.0 37.0 41.0 33.0 41.0 38-39 37.7225141760502 40.0 37.0 41.0 33.0 41.0 40-41 37.53919821654828 40.0 37.0 41.0 33.0 41.0 42-43 37.30371864898913 40.0 37.0 41.0 32.0 41.0 44-45 36.9535434917869 40.0 36.0 41.0 31.0 41.0 46-47 36.661526367317514 39.0 36.0 41.0 31.0 41.0 48-49 36.309758810162734 39.0 35.5 41.0 30.0 41.0 50-51 36.026489809817676 39.0 35.0 40.5 29.5 41.0 52-53 35.74772834512671 39.0 35.0 40.0 28.5 41.0 54-55 35.49406648184895 38.5 35.0 40.0 28.0 41.0 56-57 35.19138845139841 38.0 34.5 40.0 27.5 41.0 58-59 34.836357753604354 38.0 34.0 40.0 27.0 41.0 60-61 34.477449415886056 37.0 34.0 40.0 26.0 41.0 62-63 34.091727037334564 37.0 33.5 39.5 26.0 41.0 64-65 33.66920624986981 36.0 33.0 39.0 25.5 40.5 66-67 33.269268424336886 36.0 33.0 39.0 24.5 40.0 68-69 32.818173872957594 35.0 32.0 38.5 23.5 40.0 70-71 32.33729761599636 35.0 32.0 37.5 22.5 40.0 72-73 31.992465478000746 35.0 31.0 37.0 22.0 39.0 74-75 31.550457093278006 35.0 31.0 36.5 20.5 39.0 76-77 30.199771136375368 33.5 29.5 35.0 19.5 37.0 78-79 30.911049077526535 34.0 31.0 36.0 20.0 37.0 80-81 30.75378299707196 34.0 31.0 35.0 18.5 37.0 82-83 30.445314544840333 34.0 31.0 35.0 18.0 37.0 84-85 30.092121005188602 34.0 30.0 35.0 15.0 36.0 86-87 29.74128810280478 34.0 30.0 35.0 8.5 36.0 88-89 29.39791047601279 34.0 29.5 35.0 7.0 35.0 90-91 28.99976882690864 34.0 29.0 35.0 2.0 35.0 92-93 28.54675085201209 34.0 29.0 35.0 2.0 35.0 94-95 28.090057655157672 33.0 29.0 35.0 2.0 35.0 96-97 27.466942700771906 33.0 27.0 35.0 2.0 35.0 98-99 26.73614319961672 33.0 25.5 35.0 2.0 35.0 100-101 23.742964730367497 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3128475186803925 1101 2 0.1545013865825382 1101 3 0.2169415308374596 1101 4 0.20064008152048984 1101 5 0.24651332278493499 1101 6 0.23199334516321812 1101 7 0.2821794983418613 1101 8 0.2703880749047869 1101 9 0.34438824721714667 1101 10-11 0.3412705051786915 1101 12-13 0.3481631928491069 1101 14-15 0.3995807273575025 1101 16-17 0.445854107280077 1101 18-19 0.42120259933749793 1101 20-21 0.40031676999348775 1101 22-23 0.35856907870511634 1101 24-25 0.43798183970528726 1101 26-27 0.39370253845717684 1101 28-29 0.3291867212992301 1101 30-31 0.4151003587116193 1101 32-33 0.5128037873223619 1101 34-35 0.4905470413603581 1101 36-37 0.49622737458701494 1101 38-39 0.43021981491583716 1101 40-41 0.37270863382563846 1101 42-43 0.42950467618713617 1101 44-45 0.38538936050149886 1101 46-47 0.5266028478890163 1101 48-49 0.32665834767019675 1101 50-51 0.3616284366114897 1101 52-53 0.35359996709529895 1101 54-55 0.4299084073224435 1101 56-57 0.49606175283609133 1101 58-59 0.4434509062636991 1101 60-61 0.4144446884834494 1101 62-63 0.4887318527823652 1101 64-65 0.4543925568743461 1101 66-67 0.41014735940584046 1101 68-69 0.3607168722106735 1101 70-71 0.32006185403947285 1101 72-73 0.37694030037273407 1101 74-75 0.3118498516469579 1101 76-77 0.18433061931419914 1101 78-79 0.2799508404032771 1101 80-81 0.4513488479407357 1101 82-83 0.46475750961426954 1101 84-85 0.5804682179483436 1101 86-87 0.3828624993454177 1101 88-89 0.42106622188902065 1101 90-91 0.5560716287579233 1101 92-93 0.6112152254144902 1101 94-95 0.7467996727969712 1101 96-97 0.8050604043458662 1101 98-99 0.7214224177111852 1101 100-101 0.8464015710642911 1102 1 0.20787050694689668 1102 2 0.15234496996963998 1102 3 0.18733859193245905 1102 4 0.1580177156953937 1102 5 0.21711879549314972 1102 6 0.14709669650959256 1102 7 0.20821008144523745 1102 8 0.21405929065927864 1102 9 0.29386445214299783 1102 10-11 0.3064146898647877 1102 12-13 0.34584279929349293 1102 14-15 0.4403088342789587 1102 16-17 0.3792869998360828 1102 18-19 0.3580289587782417 1102 20-21 0.3078141929195297 1102 22-23 0.30850619919605293 1102 24-25 0.3370319510589823 1102 26-27 0.3368608042096426 1102 28-29 0.27476527303296194 1102 30-31 0.29182290397336175 1102 32-33 0.39458360058994657 1102 34-35 0.40727455180284977 1102 36-37 0.4952947942376227 1102 38-39 0.39976173111958246 1102 40-41 0.39922866927477685 1102 42-43 0.42311313567202546 1102 44-45 0.5229171989252563 1102 46-47 0.4944656949723978 1102 48-49 0.49181606130989763 1102 50-51 0.5406206692393809 1102 52-53 0.6314394863190032 1102 54-55 0.592076947959157 1102 56-57 0.5718376547572035 1102 58-59 0.537838362349369 1102 60-61 0.5640659462489097 1102 62-63 0.6112904106945081 1102 64-65 0.5787684174965477 1102 66-67 0.629084580679276 1102 68-69 0.4935991308776977 1102 70-71 0.46077817105298635 1102 72-73 0.450383005528078 1102 74-75 0.38492769954646633 1102 76-77 0.5098706546099798 1102 78-79 0.6183288667535152 1102 80-81 0.5280795817796644 1102 82-83 0.5266568541994587 1102 84-85 0.5655353615425724 1102 86-87 0.3222897590366962 1102 88-89 0.569025458658853 1102 90-91 0.8726959056161832 1102 92-93 0.928145341522491 1102 94-95 0.8840080471644818 1102 96-97 1.0190056745250935 1102 98-99 1.0429968811813666 1102 100-101 1.0861492807274686 1103 1 0.024211212635371737 1103 2 -0.19588781573125402 1103 3 -0.0679726465392747 1103 4 0.05106124703110737 1103 5 0.13084225690734996 1103 6 0.09081967961228798 1103 7 0.16980841656906165 1103 8 0.15317096935366692 1103 9 0.13451732094097935 1103 10-11 0.15949177343226495 1103 12-13 0.15098323947506032 1103 14-15 0.1878822837060241 1103 16-17 0.22819683107428546 1103 18-19 0.26182085395113575 1103 20-21 0.2114272348017181 1103 22-23 0.30027051134742777 1103 24-25 0.24029997775404865 1103 26-27 0.21499400134646152 1103 28-29 0.21100524866547943 1103 30-31 0.2390610364926431 1103 32-33 0.2587911103153644 1103 34-35 0.26630045134393754 1103 36-37 0.31351580701969084 1103 38-39 0.38319792098987904 1103 40-41 0.398350028641957 1103 42-43 0.357572941512764 1103 44-45 0.35740227017753057 1103 46-47 0.24291162273679134 1103 48-49 0.25402731008147583 1103 50-51 0.1624195401307773 1103 52-53 0.3317918522443293 1103 54-55 0.2562317700580792 1103 56-57 0.3220985986271998 1103 58-59 0.24695960140505946 1103 60-61 0.17942837285523439 1103 62-63 0.17244752546081088 1103 64-65 0.13396035686191254 1103 66-67 0.058375100926667756 1103 68-69 0.12165750014063548 1103 70-71 -0.014115096521813086 1103 72-73 0.16115632666202728 1103 74-75 0.05769146853550566 1103 76-77 6.758494837733053E-4 1103 78-79 0.1099803502087795 1103 80-81 0.25880758950072646 1103 82-83 0.29913228644780787 1103 84-85 0.3852023129464186 1103 86-87 0.08674334976226916 1103 88-89 0.28844227955408286 1103 90-91 0.47143121184265624 1103 92-93 0.3837942004267596 1103 94-95 0.21497613498364743 1103 96-97 0.5703795583059623 1103 98-99 0.8015987854480535 1103 100-101 0.9055712075246802 1104 1 0.1947966493256743 1104 2 0.037346086792503286 1104 3 0.12535699985753013 1104 4 0.0911193868394733 1104 5 0.12537938621735378 1104 6 0.13004893068258383 1104 7 0.16110827672978445 1104 8 0.15805022442225436 1104 9 0.20440470362147067 1104 10-11 0.19101205684703615 1104 12-13 0.1939836487115798 1104 14-15 0.15989090925595661 1104 16-17 0.23451160585644004 1104 18-19 0.20335530272836877 1104 20-21 0.20172265115115096 1104 22-23 0.1834852288096016 1104 24-25 0.30230602961620434 1104 26-27 0.24275673421313115 1104 28-29 0.29803369429918547 1104 30-31 0.2892943147459235 1104 32-33 0.23067961923624836 1104 34-35 0.23923473091631564 1104 36-37 0.2662533573930759 1104 38-39 0.31093189306368885 1104 40-41 0.4016860790190364 1104 42-43 0.42213739357598 1104 44-45 0.5262101487532362 1104 46-47 0.5239346401068374 1104 48-49 0.40836558673208145 1104 50-51 0.33292333111239003 1104 52-53 0.3231800779251657 1104 54-55 0.3500959438539084 1104 56-57 0.3346812775069026 1104 58-59 0.33285559082001015 1104 60-61 0.3478265109298846 1104 62-63 0.43176017611057915 1104 64-65 0.25887711519800405 1104 66-67 0.3277703246191095 1104 68-69 0.3713683802544949 1104 70-71 0.25271383119116564 1104 72-73 0.2992921300188378 1104 74-75 0.3511197617638864 1104 76-77 0.2924342695754696 1104 78-79 0.3324576826049217 1104 80-81 0.39857781898900413 1104 82-83 0.4184280905303588 1104 84-85 0.4386983956323576 1104 86-87 0.397998126708206 1104 88-89 0.47832466269488094 1104 90-91 0.6292466549100624 1104 92-93 0.6810423704541115 1104 94-95 0.7478786836065225 1104 96-97 0.7518121145827017 1104 98-99 0.5855880955720529 1104 100-101 0.5811069612892865 1105 1 0.11356787983442729 1105 2 -0.06686162108752569 1105 3 0.07501192829348469 1105 4 0.09244039627289169 1105 5 0.07588047848056334 1105 6 0.05931961279198816 1105 7 0.10803205415025019 1105 8 0.1292205098147221 1105 9 0.20201686913434713 1105 10-11 0.22588579879210613 1105 12-13 0.3421575636755634 1105 14-15 0.3811778355022639 1105 16-17 0.2873155021145095 1105 18-19 0.283357814992911 1105 20-21 0.36724529091410574 1105 22-23 0.34299539243808397 1105 24-25 0.2253488000326911 1105 26-27 0.24942429220128304 1105 28-29 0.12024345379619916 1105 30-31 0.31933756750142805 1105 32-33 0.25485740474407237 1105 34-35 0.2691787945771793 1105 36-37 0.2884325713816267 1105 38-39 0.28792702352988897 1105 40-41 0.039232661412441416 1105 42-43 0.2086016119481684 1105 44-45 0.47027144327543624 1105 46-47 0.39719996482195796 1105 48-49 0.405809347698586 1105 50-51 0.3148658874145269 1105 52-53 0.5596360338260453 1105 54-55 0.41312085234555695 1105 56-57 0.41423872061317724 1105 58-59 0.3613533276127825 1105 60-61 0.42420507172964506 1105 62-63 0.16110636419281832 1105 64-65 0.02945096453549212 1105 66-67 0.125558565564603 1105 68-69 0.03690926055672605 1105 70-71 0.09871152164309649 1105 72-73 0.05027238645913812 1105 74-75 0.11232566452071424 1105 76-77 0.02548003341235372 1105 78-79 0.024944276301543056 1105 80-81 0.09525875074336199 1105 82-83 0.2169369806734096 1105 84-85 0.2870044509786851 1105 86-87 0.3702321806315787 1105 88-89 0.46756875897180095 1105 90-91 0.49823511545193 1105 92-93 0.6715425990031427 1105 94-95 0.7256912860246878 1105 96-97 0.6208777769745772 1105 98-99 0.6271244656298016 1105 100-101 0.568007098970849 1106 1 0.3911386591835111 1106 2 0.21613816250502538 1106 3 0.18773903850132712 1106 4 0.21233953158399999 1106 5 0.20830537098251511 1106 6 0.2645656116064501 1106 7 0.23474331956927585 1106 8 0.26117730359723623 1106 9 0.33199306431504993 1106 10-11 0.33075637772908095 1106 12-13 0.37068806301223134 1106 14-15 0.5565890096537984 1106 16-17 0.5282099235068642 1106 18-19 0.5194289377712025 1106 20-21 0.44043570124144793 1106 22-23 0.45945200274471887 1106 24-25 0.4956981569262098 1106 26-27 0.42392161533523876 1106 28-29 0.49845428169582107 1106 30-31 0.5418538215825919 1106 32-33 0.5569233901977313 1106 34-35 0.5120632235908289 1106 36-37 0.583927532851682 1106 38-39 0.7055745804779718 1106 40-41 0.6411476321033405 1106 42-43 0.6873501653114218 1106 44-45 0.8323258085900207 1106 46-47 0.7137217325885459 1106 48-49 0.6678517165133329 1106 50-51 0.5797697742804715 1106 52-53 0.5071607560247458 1106 54-55 0.5630747481500791 1106 56-57 0.3760628001758519 1106 58-59 0.48006457421377036 1106 60-61 0.5155607703023009 1106 62-63 0.5479393823240031 1106 64-65 0.4986917037080545 1106 66-67 0.5073754001822692 1106 68-69 0.6160932162967043 1106 70-71 0.6690121868607761 1106 72-73 0.7174965375303479 1106 74-75 0.6044973603082902 1106 76-77 0.713316993922696 1106 78-79 0.8372660929950264 1106 80-81 0.6984474674471919 1106 82-83 0.7915458006882758 1106 84-85 0.7778788782046249 1106 86-87 0.736073676289319 1106 88-89 0.9251317288347103 1106 90-91 1.0967487255986192 1106 92-93 1.0612089081270675 1106 94-95 1.229895941607527 1106 96-97 1.508604651956933 1106 98-99 1.6969911398236839 1106 100-101 2.044426920011297 1107 1 0.21905595203448058 1107 2 0.09405702837937469 1107 3 0.14355341690298928 1107 4 0.15661699354708247 1107 5 0.10131134605130399 1107 6 0.10162156525792199 1107 7 0.1269827482471868 1107 8 0.15855325860393066 1107 9 0.18991759307354528 1107 10-11 0.2055128616104227 1107 12-13 0.2088179076217216 1107 14-15 0.25948391717212616 1107 16-17 0.3287257541721047 1107 18-19 0.2838804850212 1107 20-21 0.1414021426027645 1107 22-23 0.2628824642213843 1107 24-25 0.3089415617232021 1107 26-27 0.32548120751077647 1107 28-29 0.2704152005350764 1107 30-31 0.34166784181893206 1107 32-33 0.3203034665256652 1107 34-35 0.31109375718455823 1107 36-37 0.20872213229943526 1107 38-39 0.28126725527112484 1107 40-41 0.3172436663904108 1107 42-43 0.2362557645486305 1107 44-45 0.23457813605961064 1107 46-47 0.2644959020926976 1107 48-49 0.16082483959394978 1107 50-51 0.23362840061965784 1107 52-53 0.3638646791450455 1107 54-55 0.2994378455057074 1107 56-57 0.3120858219779521 1107 58-59 0.3181829236524436 1107 60-61 0.4848229860388287 1107 62-63 0.3472470100353462 1107 64-65 0.35671817648144355 1107 66-67 0.2199395613857078 1107 68-69 0.30214800107080464 1107 70-71 0.3352828751432 1107 72-73 0.43093644924743657 1107 74-75 0.371724991310888 1107 76-77 0.3285639464924124 1107 78-79 0.4822561950479418 1107 80-81 0.5346830289076365 1107 82-83 0.4209765840297166 1107 84-85 0.507363930852506 1107 86-87 0.5344272048227516 1107 88-89 0.4598235493551641 1107 90-91 0.6778529033066967 1107 92-93 0.7130192765291419 1107 94-95 0.7083427988278252 1107 96-97 1.0659649493309509 1107 98-99 0.9361778141376682 1107 100-101 1.1558753236345787 1108 1 0.040277942611467665 1108 2 -0.10008167255067946 1108 3 0.01096619216872341 1108 4 0.08532023024056912 1108 5 0.04394438356790431 1108 6 0.0667151167032145 1108 7 0.01781014850698881 1108 8 0.04446221356260338 1108 9 0.09446178844724074 1108 10-11 0.12280263435651051 1108 12-13 0.14415053314333903 1108 14-15 0.11538500305978516 1108 16-17 0.15988865497877214 1108 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-1.0760119494777314 2316 54-55 -1.1068015852776583 2316 56-57 -1.3336258756343184 2316 58-59 -1.223094712723885 2316 60-61 -0.6973091553513413 2316 62-63 -0.8156488170452434 2316 64-65 -0.8931730144250167 2316 66-67 -0.8900383231660314 2316 68-69 -0.8970050561473641 2316 70-71 -0.9584928366722849 2316 72-73 -0.8192031890892686 2316 74-75 -0.8500948135762734 2316 76-77 -1.1277120260115332 2316 78-79 -0.7679908922686316 2316 80-81 -0.875190398914306 2316 82-83 -0.7841804682956734 2316 84-85 -0.6084946318337714 2316 86-87 -0.6752472383693835 2316 88-89 -0.8889859884872777 2316 90-91 -0.8808768049307858 2316 92-93 -1.0285777145367234 2316 94-95 -0.7713609049430445 2316 96-97 -0.675526859611157 2316 98-99 -0.7675599595738767 2316 100-101 -0.6118678125517896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 10.0 5 45.0 6 92.0 7 244.0 8 588.0 9 1098.0 10 1954.0 11 3082.0 12 4773.0 13 6435.0 14 8231.0 15 11568.0 16 15637.0 17 21774.0 18 19648.0 19 12355.0 20 11060.0 21 11327.0 22 11808.0 23 12831.0 24 14597.0 25 17248.0 26 21070.0 27 26519.0 28 32971.0 29 41637.0 30 52028.0 31 65990.0 32 85258.0 33 112028.0 34 155856.0 35 225101.0 36 348233.0 37 502323.0 38 330279.0 39 22601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.759533687204474 18.703509121542055 14.93813293719089 22.598824254062574 2 16.08399525409606 20.361694034108922 39.11747923667026 24.436831475124762 3 19.887819961272253 24.234411539346336 29.39563524913098 26.482133250250428 4 13.976697880765132 16.74118110018716 35.676088859424254 33.60603215962345 5 14.70726752532082 36.788388334355254 33.10078242527381 15.403561715050115 6 32.7706661981296 35.244025699499886 16.914248659144715 15.071059443225806 7 29.176412092373276 32.38598361364687 20.035040513046003 18.40256378093385 8 27.169064738427988 34.18187367488686 19.1149580084608 19.534103578224354 9 26.867113284324333 16.64183612567484 19.69834741806148 36.792703171939344 10-11 25.592876873613186 26.28374767921025 26.38636054883847 21.73701489833809 12-13 26.607646640613325 23.512967046900542 27.2188888234788 22.66049748900733 14-15 23.59471213629295 24.739369886908584 25.645405383865068 26.020512592933393 16-17 22.900355114472568 27.984193285157556 26.0973816081315 23.01806999223838 18-19 22.861864002296787 26.297422182292095 28.69319957143543 22.14751424397569 20-21 23.695206413065232 25.32910795103073 28.830762208127553 22.144923427776483 22-23 23.570643870267745 25.177217608823433 28.8107559563864 22.441382564522424 24-25 23.2433742032628 25.444191601852168 28.852698434262813 22.45973576062222 26-27 23.068604637735373 25.5372512543278 28.858222636928847 22.535921471007985 28-29 22.909177609859878 25.747977331989265 28.79597250638749 22.546872551763364 30-31 23.052582712470844 25.503226975248534 28.724201182091985 22.719989130188637 32-33 23.124025542062395 25.576953817882615 28.759115129926972 22.539905510128023 34-35 23.35366165681497 25.579384390075578 28.570147659978563 22.49680629313089 36-37 23.451835290830463 25.503601430926594 28.55615123405984 22.488412044183107 38-39 23.17601979300846 25.57384123976652 28.639112928333216 22.611026038891804 40-41 23.33830949297741 25.572441389360677 28.43885425905451 22.6503948586074 42-43 23.143225308610525 25.668466512652927 28.211391446916316 22.976916731820232 44-45 23.324577675949204 25.656996952161776 28.20600950103168 22.812415870857336 46-47 23.608583712754413 26.08775489364323 27.558638472108733 22.74502292149363 48-49 23.781791785470645 26.152308942489327 27.212267204396877 22.85363206764315 50-51 23.50513167049805 26.38085510709273 27.28988158972312 22.8241316326861 52-53 23.769354499807996 26.410129730395532 26.870851960265764 22.94966380953071 54-55 23.535461351015137 26.38193822085742 26.923233679962287 23.15936674816515 56-57 23.495747159905246 26.614926760039108 26.931665194184273 22.95766088587137 58-59 23.814227996083805 26.282159848496512 26.8911218123179 23.01249034310178 60-61 23.63092170627377 26.239837085434143 27.372826017061286 22.756415191230797 62-63 23.34682937388093 26.32087911888863 27.43062316657776 22.90166834065268 64-65 23.402309562527144 26.397921112966237 27.238319845564252 22.96144947894237 66-67 23.359376751206078 26.47894907371034 27.07861863845914 23.083055536624446 68-69 23.162874988509266 26.40165448460151 27.300445002741924 23.135025524147295 70-71 23.381980981250464 26.372252432816367 27.06772126634378 23.178045319589394 72-73 23.491521309821444 26.429413381599698 26.871789669977055 23.207275638601804 74-75 23.400754972621375 26.77123315213749 26.678220090459053 23.149791784782085 76-77 23.556049453828816 26.599297553482753 26.59787110523022 23.246781887458216 78-79 23.450740548033167 26.63729108901565 26.638649601933075 23.273318761018107 80-81 23.424528250619066 26.76558444028614 26.618290127001437 23.19159718209336 82-83 23.486471518163338 26.801967334565973 26.567575388302227 23.14398575896846 84-85 23.444098212995893 26.773248692046675 26.387191318260268 23.395461776697168 86-87 23.360594677698003 26.707717179401836 26.635035355971116 23.296652786929045 88-89 23.514044440097162 26.712399174740835 26.2922071335286 23.4813492516334 90-91 23.54071537850731 26.621728619691304 26.389626950511015 23.44792905129037 92-93 23.346387109365978 26.55453219466006 26.53967914700034 23.55940154897362 94-95 23.461082576056892 26.517871799429198 26.52595494945767 23.495090675056236 96-97 23.337921396715476 26.572426833446055 26.53110482815367 23.558546941684803 98-99 23.233574748293996 26.70492806232681 26.210142870016767 23.85135431936242 100-101 22.973720467649557 26.936356504107838 26.223764902735276 23.866158125507326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 498.0 1 373.5 2 277.0 3 311.5 4 291.5 5 281.5 6 470.5 7 1062.5 8 1812.0 9 2270.5 10 2254.0 11 2171.0 12 2312.0 13 2502.5 14 2767.5 15 2955.0 16 3134.0 17 3438.0 18 3697.0 19 3865.0 20 4112.0 21 4506.0 22 5117.5 23 6023.0 24 7252.5 25 8770.0 26 10530.5 27 12651.0 28 14809.5 29 17241.0 30 20338.5 31 23795.0 32 26479.5 33 28833.5 34 32429.0 35 35577.0 36 39183.0 37 43213.0 38 46257.5 39 49054.0 40 52271.5 41 57219.0 42 61649.0 43 65980.5 44 71888.0 45 76519.5 46 80514.0 47 84987.5 48 89577.0 49 89717.0 50 86863.5 51 86127.5 52 86614.0 53 86881.5 54 85835.0 55 82785.0 56 77115.5 57 70890.0 58 65929.5 59 59566.0 60 50298.5 61 40599.5 62 32327.5 63 25796.0 64 19359.0 65 14201.0 66 10654.0 67 8055.5 68 5648.5 69 3488.0 70 2311.5 71 1458.5 72 876.0 73 590.5 74 388.0 75 242.0 76 161.0 77 88.5 78 51.0 79 33.5 80 22.0 81 13.0 82 11.5 83 9.5 84 7.5 85 6.0 86 2.5 87 0.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015487012193078664 2 0.003713260233428218 3 0.0029887216512958824 4 2.2641830691635473E-4 5 0.0018113464553308378 6 4.5283661383270946E-4 7 4.528366138327095E-5 8 0.0 9 0.0 10-11 9.05673227665419E-5 12-13 5.434039365992514E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002717019682996257 22-23 0.0 24-25 0.003486841926511863 26-27 0.00215097391570537 28-29 0.0036226929106616757 30-31 0.015939848806911374 32-33 0.0010415242118152316 34-35 0.0024000340533133603 36-37 0.0037359020641198532 38-39 0.00215097391570537 40-41 0.004822709937318356 42-43 0.00260381052953808 44-45 7.018967514406996E-4 46-47 1.5849281484144832E-4 48-49 3.396274603745321E-4 50-51 2.490601376079902E-4 52-53 0.0013585098414981285 54-55 0.003667976572044947 56-57 5.886875979825223E-4 58-59 0.0016302118097977541 60-61 0.0012679425187315865 62-63 0.0 64-65 2.2641830691635473E-4 66-67 1.1320915345817737E-4 68-69 4.528366138327095E-5 70-71 3.396274603745321E-4 72-73 4.528366138327095E-5 74-75 4.5283661383270946E-4 76-77 9.056732276654189E-4 78-79 2.2641830691635473E-4 80-81 0.00624914527089139 82-83 0.0015849281484144832 84-85 0.003667976572044947 86-87 0.0021283320850137345 88-89 0.0011773751959650446 90-91 1.1320915345817737E-4 92-93 4.528366138327095E-5 94-95 2.0377647622471927E-4 96-97 0.0016075699791061185 98-99 0.003215139958212237 100-101 0.0011094497038901382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2208302.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.13514853657236 #Duplication Level Percentage of deduplicated Percentage of total 1 73.46122073560701 37.564504339931936 2 13.994330589801008 14.312043467587454 3 4.918868777540112 7.545812567142651 4 2.3014352652406633 4.7073693654154845 5 1.3260020045041925 3.3902654730057282 6 0.8563166774650358 2.627272829791124 7 0.5770560311193307 2.0655492105648325 8 0.41143044545080215 1.683084555247592 9 0.31308507491164494 1.4408686629171763 >10 1.6914404532308063 15.487074057376896 >50 0.10512211486724415 3.6707592297699367 >100 0.04111873629848354 3.583440539186262 >500 0.0020407296953301795 0.6738482587059326 >1k 3.5490951223130337E-4 0.4625107156778737 >5k 1.7745475611565168E-4 0.7855967276792608 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9521 0.4311457400301226 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7794 0.35294085682121373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4863 0.2202144453068466 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2218 0.10043916094809495 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.5283661383270945E-5 0.0 0.0 0.0 0.0 6 2.717019682996257E-4 0.0 0.0 0.0 4.5283661383270945E-5 7 6.339712593657933E-4 0.0 0.0 0.0 4.5283661383270945E-5 8 6.339712593657933E-4 0.0 0.0 0.0 4.5283661383270945E-5 9 6.339712593657933E-4 0.0 0.0 0.0 4.5283661383270945E-5 10-11 6.566130900574288E-4 0.0 0.0 0.0 4.5283661383270945E-5 12-13 7.924640742072416E-4 0.0 0.0 4.5283661383270945E-5 4.5283661383270945E-5 14-15 9.735987197403254E-4 0.0 0.0 4.5283661383270945E-5 9.056732276654189E-5 16-17 0.0010641660425068672 0.0 0.0 4.5283661383270945E-5 9.056732276654189E-5 18-19 0.0011773751959650446 0.0 0.0 4.5283661383270945E-5 9.056732276654189E-5 20-21 0.0012226588573483156 0.0 0.0 4.5283661383270945E-5 9.056732276654189E-5 22-23 0.0012679425187315865 0.0 0.0 1.811346455330838E-4 9.056732276654189E-5 24-25 0.0014943608256479412 0.0 0.0 2.490601376079902E-4 9.056732276654189E-5 26-27 0.0014943608256479412 0.0 0.0 4.7547844452434495E-4 9.056732276654189E-5 28-29 0.0019924811008639218 0.0 0.0 0.0011320915345817738 9.056732276654189E-5 30-31 0.0020830484236304637 0.0 0.0 0.0024000340533133603 9.056732276654189E-5 32-33 0.0021056902543220993 0.0 0.0 0.004324589662102376 9.056732276654189E-5 34-35 0.0023321085612384536 0.0 0.0 0.0061132942867415776 1.3585098414981285E-4 36-37 0.002626452360229715 0.0 0.0 0.00889823946181274 1.3585098414981285E-4 38-39 0.002807587005762799 0.0 0.0 0.012837918002157313 1.3585098414981285E-4 40-41 0.002807587005762799 0.0 0.0 0.017366284140484408 1.3585098414981285E-4 42-43 0.002898154328529341 0.0 0.0 0.021985217601578046 1.3585098414981285E-4 44-45 0.003056647143370789 0.0 0.0 0.026060747126072432 1.3585098414981285E-4 46-47 0.0032377817889038727 0.0 0.0 0.03208347409004747 1.3585098414981285E-4 48-49 0.003373632773053686 0.0 0.0 0.03618164544523349 1.3585098414981285E-4 50-51 0.003441558265128592 0.0 0.0 0.040257174969727874 1.3585098414981285E-4 52-53 0.0034642000958202274 0.0 0.0 0.05012901315128093 1.8113464553308378E-4 54-55 0.003577409249278405 0.0 0.0 0.06416694818009493 2.2641830691635473E-4 56-57 0.0036226929106616757 0.0 0.0 0.08868805081913615 2.490601376079902E-4 58-59 0.0036226929106616757 0.0 0.0 0.1254810256930438 2.717019682996257E-4 60-61 0.0036226929106616757 0.0 0.0 0.16693821768942835 2.717019682996257E-4 62-63 0.0036226929106616757 0.0 0.0 0.1933385922758753 2.717019682996257E-4 64-65 0.0036226929106616757 0.0 0.0 0.22598811213321368 2.717019682996257E-4 66-67 0.0038491112175780304 0.0 0.0 0.2884569230114359 3.1698562968289663E-4 68-69 0.0038943948789613016 0.0 0.0 0.37447323780895914 3.1698562968289663E-4 70-71 0.003917036709652937 0.0 0.0 0.45965180487089174 3.396274603745321E-4 72-73 0.0039849622017278435 0.0 0.0 0.5605890860942027 3.6226929106616756E-4 74-75 0.004075529524494385 0.0 0.0 0.6575187632850941 3.6226929106616756E-4 76-77 0.004120813185877656 0.0 0.0 0.7455275591834812 4.0755295244943854E-4 78-79 0.004166096847260927 0.0 0.0 0.8274230607951267 4.0755295244943854E-4 80-81 0.005184979228384524 0.0 0.0 0.8975448104471218 4.0755295244943854E-4 82-83 0.00525290472045943 0.0 0.0 0.969274130078223 4.7547844452434495E-4 84-85 0.00525290472045943 0.0 0.0 1.1303481136185178 4.981202752159804E-4 86-87 0.0052981883818427 0.0 0.0 1.3791591910888998 5.434039365992513E-4 88-89 0.0053434720432259716 0.0 0.0 1.6926126951839016 5.886875979825223E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7360 0.0 36.33755 1 GTATCAA 11400 0.0 35.294224 1 TATCAAC 14155 0.0 28.220327 2 ATCAACG 14290 0.0 27.820768 3 TCAACGC 14445 0.0 27.5545 4 CAACGCA 14640 0.0 27.187485 5 AACGCAG 14875 0.0 26.75736 6 ACGCAGA 16765 0.0 23.54256 7 CGCAGAG 16835 0.0 23.388245 8 GAGTACT 9075 0.0 22.949816 12-13 GTACATG 9570 0.0 22.585232 1 TACATGG 9605 0.0 21.956419 2 GCAGAGT 17995 0.0 21.51107 9 AGTACTT 9535 0.0 21.294708 12-13 GTACTTT 9815 0.0 21.171606 14-15 AGAGTAC 14420 0.0 20.981203 10-11 ACATGGG 9620 0.0 20.490328 3 CGTAACG 60 0.004767353 19.790411 14-15 TACTTTT 10680 0.0 18.923191 14-15 GTATAGG 635 0.0 18.702143 1 >>END_MODULE