FastQCFastQC Report
Thu 26 May 2016
SRR937094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061443
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.37565918630784356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.27558365669096846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.166727869749491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.039.013391
GTATCAA90300.037.1559831
TATCAAC110850.030.0970382
ATCAACG111050.030.0413763
TCAACGC111400.029.946994
CAACGCA113100.029.54395
AACGCAG115750.028.9900026
ACGCAGA130200.025.6284477
CGCAGAG130100.025.3923958
GTACATG87550.023.5040951
GCAGAGT138300.022.9937959
GAGTACT64850.022.97526212-13
TACATGG88200.022.6848472
ACATGGG90200.021.1270623
AGAGTAC112350.020.92031910-11
AGTACTT68000.020.6529212-13
GTACTTT71050.020.6019814-15
CAGAGTA133650.019.34661710-11
CATGGGA41900.018.2605194
TACTTTT80300.017.51856414-15
Produced by FastQC (version 0.11.5)