FastQCFastQC Report
Thu 26 May 2016
SRR937094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061443
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87230.4231501913950568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72080.3496579822968668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.21863325835349318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.037.3771171
GTATCAA103200.034.9917561
TATCAAC127250.027.8481862
ATCAACG127950.027.5466633
TCAACGC128000.027.5325624
CAACGCA131500.026.871995
AACGCAG134950.026.2182966
ACGCAGA152450.023.1463477
CGCAGAG152850.022.8682778
GTACATG89650.022.207291
TACATGG90950.021.622882
GAGTACT81900.021.51428212-13
GCAGAGT164050.021.104379
ACATGGG91150.020.4284173
AGTACTT85900.020.37423112-13
AGAGTAC134400.020.07105310-11
GTATAGG5450.020.052251
GTACTTT87350.019.98237614-15
TAACCCG1250.00603601618.9982095
TACTTTT95800.018.14547214-15
Produced by FastQC (version 0.11.5)