FastQCFastQC Report
Thu 26 May 2016
SRR937093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132677
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75810.3554687371786726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.2700362033256794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.16594167799437046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90950.039.7594341
GGTATCA54150.039.225391
TATCAAC113500.031.7359812
ATCAACG113950.031.4855463
TCAACGC114350.031.375414
CAACGCA116750.030.689735
AACGCAG120300.029.7424966
ACGCAGA133300.026.5917267
CGCAGAG136150.025.9652888
GTACATG93200.024.1158981
TATAGCG1801.9446452E-723.7601185
GCAGAGT143600.023.7562189
TACATGG94350.023.2690532
GAGTACT67000.022.97508412-13
ATTCGCG2350.022.2422180-81
ACATGGG95450.022.005183
AGAGTAC119150.021.6928810-11
AGTACTT70950.021.29421412-13
GTACTTT72100.021.2159114-15
CGCGGAA2700.019.35578382-83
Produced by FastQC (version 0.11.5)