FastQCFastQC Report
Thu 26 May 2016
SRR937093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132677
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89390.4191445774489057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73250.3434650441674947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45470.2132062192258837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.038.9393351
GTATCAA100950.034.4440421
TATCAAC128050.026.9632682
ATCAACG129250.026.6768153
TCAACGC130550.026.4111714
CAACGCA133250.025.8863285
AACGCAG136400.025.3233456
GTACATG93400.023.140561
TACATGG95150.022.3607792
ACGCAGA154050.022.267767
GAGTACT83250.021.96312912-13
CGCAGAG156850.021.5976288
ACATGGG96250.021.4642853
GTACTTT88850.020.47242414-15
GCAGAGT165400.020.1939329
AGTACTT86850.019.84972812-13
AGAGTAC135900.019.75239810-11
CGTTTAA3506.366463E-1119.0006891
CATGGGG55150.018.7732264
CAGAGTA159600.017.96533210-11
Produced by FastQC (version 0.11.5)