FastQCFastQC Report
Thu 26 May 2016
SRR937092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093008
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75810.36220597341242844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.27104530895247414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.16411786290353406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57450.039.2254831
GTATCAA91500.038.2416571
TATCAAC115450.030.27092
ATCAACG116000.030.1273753
TCAACGC115750.030.1102894
CAACGCA118000.029.3749735
AACGCAG121100.028.5796476
ACGCAGA134750.025.5020587
CGCAGAG136350.024.994258
GCAGAGT141150.022.9977199
GAGTACT64600.022.84437212-13
GTACATG88600.022.6442891
TACATGG90750.021.4817282
AGAGTAC116150.021.29735410-11
GTACTTT69200.021.22253814-15
ACATGGG91850.020.1373543
AGTACTT68150.020.01548812-13
ATTCGCG2650.019.72627380-81
CTATACG1250.00599769419.0191864
CAGAGTA138550.018.8317210-11
Produced by FastQC (version 0.11.5)