##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937092_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2093008 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.714327417764288 33.0 31.0 34.0 30.0 34.0 2 31.805883207326488 33.0 31.0 34.0 30.0 34.0 3 31.832186021267 33.0 31.0 34.0 30.0 34.0 4 35.418460416778146 37.0 35.0 37.0 33.0 37.0 5 35.40897120316788 37.0 35.0 37.0 33.0 37.0 6 35.46844684779036 37.0 35.0 37.0 33.0 37.0 7 35.408383054436484 37.0 35.0 37.0 33.0 37.0 8 35.37648589971945 37.0 35.0 37.0 33.0 37.0 9 36.99218206523816 39.0 37.0 39.0 33.0 39.0 10-11 36.85786867513168 39.0 37.0 39.0 33.0 39.0 12-13 36.766461714432054 39.0 37.0 39.0 33.0 39.0 14-15 37.92043556450811 40.0 38.0 41.0 33.0 41.0 16-17 37.840595688119684 40.0 38.0 41.0 33.0 41.0 18-19 37.775542425064785 40.0 38.0 41.0 32.0 41.0 20-21 37.6981578665729 40.0 37.5 41.0 32.0 41.0 22-23 37.58526675483324 40.0 37.0 41.0 32.0 41.0 24-25 37.540776719439194 40.0 37.0 41.0 32.0 41.0 26-27 37.56868822288305 40.0 37.0 41.0 32.0 41.0 28-29 37.49393217799454 40.0 37.0 41.0 32.0 41.0 30-31 37.34591148242147 40.0 37.0 41.0 31.5 41.0 32-33 37.15393706091902 40.0 36.5 41.0 31.0 41.0 34-35 36.9942711637987 39.5 36.0 41.0 30.5 41.0 36-37 36.81628450536262 39.0 36.0 41.0 30.0 41.0 38-39 36.656129838013044 39.0 36.0 41.0 30.0 41.0 40-41 36.468230651770085 39.0 35.0 41.0 30.0 41.0 42-43 36.24883803597502 39.0 35.0 41.0 30.0 41.0 44-45 35.99001938836354 39.0 35.0 40.0 29.0 41.0 46-47 35.6510221174501 39.0 35.0 40.0 27.5 41.0 48-49 35.41188184660545 38.5 35.0 40.0 27.0 41.0 50-51 34.71907274124131 38.0 34.0 39.5 26.0 40.5 52-53 34.89041704570647 38.0 34.5 40.0 26.5 40.5 54-55 35.254922341433954 39.0 35.0 40.0 27.0 41.0 56-57 35.10150606208863 38.5 35.0 40.0 26.0 41.0 58-59 34.852782932506706 38.0 34.0 40.0 26.0 41.0 60-61 34.5436582182199 38.0 34.0 40.0 26.0 41.0 62-63 34.208727104722 37.0 34.0 40.0 25.0 41.0 64-65 33.85266085939471 37.0 33.5 40.0 24.5 41.0 66-67 33.457796864608255 36.0 33.0 39.0 23.0 41.0 68-69 33.05255044414546 36.0 33.0 39.0 22.0 41.0 70-71 32.63155515889093 35.0 32.0 38.5 21.5 40.0 72-73 32.24268588557712 35.0 32.0 37.5 20.5 40.0 74-75 31.83880400839366 35.0 32.0 37.0 19.5 39.0 76-77 31.391227123833257 35.0 31.0 36.5 18.0 39.0 78-79 30.943417320908473 35.0 31.0 36.0 16.0 38.5 80-81 30.535305168446563 34.5 31.0 35.5 11.0 37.0 82-83 30.150718248568566 34.0 30.0 35.0 7.5 37.0 84-85 29.80368804132617 34.0 30.0 35.0 7.0 36.5 86-87 29.460492267588084 34.0 30.0 35.0 2.0 36.0 88-89 29.112214573475114 34.0 29.0 35.0 2.0 36.0 90-91 28.756846605459703 34.0 29.0 35.0 2.0 35.0 92-93 28.383602929372465 34.0 29.0 35.0 2.0 35.0 94-95 28.015908443732656 34.0 29.0 35.0 2.0 35.0 96-97 27.516117950815286 34.0 27.0 35.0 2.0 35.0 98-99 26.929135244585783 34.0 26.5 35.0 2.0 35.0 100-101 24.686488059290745 31.0 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20756154907317992 1101 2 0.2582036837599908 1101 3 0.27560534083403354 1101 4 0.2785341799141676 1101 5 0.2414552264851224 1101 6 0.2214259662376179 1101 7 0.18594089392806978 1101 8 0.17648735108599567 1101 9 0.10483945945630069 1101 10-11 0.11795614397600929 1101 12-13 0.23870966863795218 1101 14-15 0.4001713911197484 1101 16-17 0.31094583551040955 1101 18-19 0.32671205522007085 1101 20-21 0.3498805879832574 1101 22-23 0.39714189350459606 1101 24-25 0.38276094754520074 1101 26-27 0.3678539739946416 1101 28-29 0.33774144940719 1101 30-31 0.24957496063617413 1101 32-33 0.14899839230204037 1101 34-35 0.25331077902837507 1101 36-37 0.40803107646543424 1101 38-39 0.2274985354815371 1101 40-41 0.3133386328534513 1101 42-43 0.4088789988740942 1101 44-45 0.3786285904849436 1101 46-47 0.30817604984120806 1101 48-49 0.3850846991178898 1101 50-51 0.5726826045010185 1101 52-53 0.5802035333355633 1101 54-55 0.5428043027669744 1101 56-57 0.5360977640285611 1101 58-59 0.5474134699896993 1101 60-61 0.599559905706009 1101 62-63 0.5027845343251016 1101 64-65 0.38384881535037607 1101 66-67 0.5080923636743435 1101 68-69 0.5140068826836384 1101 70-71 0.3930747892861106 1101 72-73 0.4223798921636188 1101 74-75 0.44809530027784206 1101 76-77 0.3845652383470366 1101 78-79 0.5257914947471605 1101 80-81 0.5790655476923021 1101 82-83 0.41442360071806306 1101 84-85 0.3776035096116068 1101 86-87 0.45706710463926825 1101 88-89 0.5446261405677824 1101 90-91 0.6719125224025007 1101 92-93 0.745410289112538 1101 94-95 0.6088704752271781 1101 96-97 0.516892805269773 1101 98-99 0.6056386104047071 1101 100-101 0.9215346295842473 1102 1 0.13744505950214503 1102 2 0.1669443370460968 1102 3 0.15542509683560368 1102 4 0.21930474268473432 1102 5 0.21202429378146093 1102 6 0.12742754042101012 1102 7 0.08968587621851043 1102 8 0.02900683160547146 1102 9 0.10131092738231473 1102 10-11 0.12327373547541498 1102 12-13 0.06937515466707822 1102 14-15 -0.08327764469292731 1102 16-17 0.1453256072538167 1102 18-19 -0.1053843635127123 1102 20-21 -0.08857041555865663 1102 22-23 -0.09236617418529391 1102 24-25 -0.21485770652800085 1102 26-27 -0.14812752935049645 1102 28-29 -0.2519460751894229 1102 30-31 -0.11013617671132891 1102 32-33 -0.03017988396714344 1102 34-35 -0.08514061805938411 1102 36-37 -0.019976400905683533 1102 38-39 0.06868468266767991 1102 40-41 -5.499936715338549E-4 1102 42-43 0.003152513238518395 1102 44-45 0.22358648107920231 1102 46-47 0.12705247644490214 1102 48-49 0.23313074398212308 1102 50-51 0.20867788195084103 1102 52-53 0.3966069178298568 1102 54-55 0.04317502295587872 1102 56-57 0.15954247083690376 1102 58-59 -0.09147279526020213 1102 60-61 -0.14908314820976898 1102 62-63 -0.2475039347815482 1102 64-65 0.051318315547149496 1102 66-67 -0.14576871464127805 1102 68-69 -0.02966568717075546 1102 70-71 -0.07334630477134851 1102 72-73 0.019865134194311906 1102 74-75 0.1758709791444346 1102 76-77 -0.09514865382140059 1102 78-79 0.009569141342989695 1102 80-81 0.0794673580031997 1102 82-83 0.11449443897071987 1102 84-85 0.13282940492841178 1102 86-87 0.19606002081400575 1102 88-89 0.295936648241927 1102 90-91 0.20946151886058573 1102 92-93 0.2248758538508291 1102 94-95 0.2898177400835351 1102 96-97 0.17828949948464867 1102 98-99 0.34695856317920715 1102 100-101 0.19822530254764814 1103 1 0.27875758484789515 1103 2 0.21629408743414658 1103 3 0.18463458908155417 1103 4 0.22581143396299552 1103 5 0.14852846796445363 1103 6 0.14716925847837103 1103 7 0.19838034280406447 1103 8 0.17829155703233113 1103 9 0.11247494410701364 1103 10-11 0.19489208789671864 1103 12-13 0.029124703687507747 1103 14-15 0.2913828970419061 1103 16-17 0.31801931871079603 1103 18-19 0.30808768973783174 1103 20-21 0.15707710720008095 1103 22-23 0.2945603126489047 1103 24-25 0.3145066569964925 1103 26-27 0.35247132956631333 1103 28-29 0.38497446850315953 1103 30-31 0.380193283092062 1103 32-33 0.3785502414680977 1103 34-35 0.41240311647096206 1103 36-37 0.3265000153100104 1103 38-39 0.450245211817041 1103 40-41 0.4871875571947655 1103 42-43 0.5410382930486222 1103 44-45 0.48917149597200904 1103 46-47 0.47178615378125244 1103 48-49 0.54414198700767 1103 50-51 0.5420077193185264 1103 52-53 0.6402994965939683 1103 54-55 0.656224413958661 1103 56-57 0.6632698694189472 1103 58-59 0.6688553360762342 1103 60-61 0.6681509136828083 1103 62-63 0.6502027117483564 1103 64-65 0.632623289607082 1103 66-67 0.6511544859851952 1103 68-69 0.6762309581006036 1103 70-71 0.6253549439875883 1103 72-73 0.6550170688617101 1103 74-75 0.7207979833815443 1103 76-77 0.6937334762005598 1103 78-79 0.7920540038116997 1103 80-81 0.7364554437522486 1103 82-83 0.7458920561205282 1103 84-85 0.7173310899838548 1103 86-87 0.7679244892174673 1103 88-89 0.6455954419939367 1103 90-91 0.654516825037259 1103 92-93 0.536056651210675 1103 94-95 0.7758898129114904 1103 96-97 0.9011296919026002 1103 98-99 0.7604991373566072 1103 100-101 0.8753651831254601 1104 1 0.14811034681009616 1104 2 0.1808628068151492 1104 3 0.19876988585996003 1104 4 0.2744266834502369 1104 5 0.21453207702732158 1104 6 0.07027085072606809 1104 7 0.09829167186300936 1104 8 0.11166509743205921 1104 9 0.1747611944869476 1104 10-11 0.29691512558845545 1104 12-13 0.1172933555780773 1104 14-15 0.17501156085100433 1104 16-17 0.32777940458159804 1104 18-19 0.3504310556915442 1104 20-21 0.27532896139202734 1104 22-23 0.21206881476815198 1104 24-25 0.27712964155745823 1104 26-27 0.3114164445274099 1104 28-29 0.2827315484170967 1104 30-31 0.514800797505572 1104 32-33 0.2239385148857238 1104 34-35 0.20985014725091133 1104 36-37 0.30635639471154263 1104 38-39 0.4937050418464608 1104 40-41 0.4207426875446387 1104 42-43 0.4055994137727268 1104 44-45 0.375200019056372 1104 46-47 0.4028672332452601 1104 48-49 0.5431045010980924 1104 50-51 0.34891268465189285 1104 52-53 0.5263779793515937 1104 54-55 0.6536256134694725 1104 56-57 0.7011557555419969 1104 58-59 0.6378142243508478 1104 60-61 0.6008743800105876 1104 62-63 0.49253088038827997 1104 64-65 0.48424485495433345 1104 66-67 0.29634507465973314 1104 68-69 0.28188429899669387 1104 70-71 0.36202528433561554 1104 72-73 0.5441903589245811 1104 74-75 0.5454870966003327 1104 76-77 0.5571404387242147 1104 78-79 0.6659725414232582 1104 80-81 0.5304450856460932 1104 82-83 0.47938352528194983 1104 84-85 0.5745502328553549 1104 86-87 0.6657120834228643 1104 88-89 0.568284792147228 1104 90-91 0.46826895804606394 1104 92-93 0.6287351358829305 1104 94-95 0.5857549636760204 1104 96-97 0.47389610559980255 1104 98-99 0.531788539683351 1104 100-101 0.6421268030873115 1105 1 0.1640506934828565 1105 2 0.13430391906551975 1105 3 0.19376306831626522 1105 4 0.16924916911922594 1105 5 0.235343298677833 1105 6 0.21136362240044093 1105 7 0.26600901124122345 1105 8 0.2663754232787028 1105 9 0.2791002362071424 1105 10-11 0.2851864765573353 1105 12-13 0.3436047029634537 1105 14-15 0.501330161708978 1105 16-17 0.4134675345619101 1105 18-19 0.3303072326695542 1105 20-21 0.4069794890821541 1105 22-23 0.46197172151322974 1105 24-25 0.37066401136879534 1105 26-27 0.4928145057366322 1105 28-29 0.4206881539168137 1105 30-31 0.4883778619291448 1105 32-33 0.4686627544299782 1105 34-35 0.12496398233721351 1105 36-37 0.31754091320706124 1105 38-39 0.5556457348605264 1105 40-41 0.4972056973562857 1105 42-43 0.47284018559025043 1105 44-45 0.37682532531744073 1105 46-47 0.3518754409578122 1105 48-49 0.40883551783559824 1105 50-51 0.213492152568854 1105 52-53 0.4493122347886356 1105 54-55 0.38924866635041155 1105 56-57 0.35475104011229774 1105 58-59 0.3917387253083362 1105 60-61 0.42085976258203317 1105 62-63 0.2075628376994345 1105 64-65 0.5256075422746633 1105 66-67 0.47854798311009716 1105 68-69 0.38904955303756594 1105 70-71 0.31339771580771014 1105 72-73 0.5464290880008917 1105 74-75 0.6667495466936799 1105 76-77 0.5308722999388671 1105 78-79 0.6767827374999911 1105 80-81 0.4833614019484216 1105 82-83 0.4495651479125904 1105 84-85 0.6630876325526813 1105 86-87 0.5390812229712694 1105 88-89 0.5314742593364308 1105 90-91 0.5311958351162751 1105 92-93 0.5684512128686912 1105 94-95 0.6952284792540588 1105 96-97 0.5453247971189832 1105 98-99 0.4820627425639401 1105 100-101 0.5210613015877392 1106 1 0.3547262038550727 1106 2 0.35529431184110294 1106 3 0.3491999260659995 1106 4 0.37819724917097375 1106 5 0.30628726741565515 1106 6 0.4075532878993542 1106 7 0.3661138893713485 1106 8 0.43692767405345734 1106 9 0.5109057050927746 1106 10-11 0.5713167643293531 1106 12-13 0.5016913085239025 1106 14-15 0.36711337955473766 1106 16-17 0.5087833391744354 1106 18-19 0.45902468346106673 1106 20-21 0.4261779738654212 1106 22-23 0.3115773666306154 1106 24-25 0.37626354567454 1106 26-27 0.30449298057656904 1106 28-29 0.2990276699950414 1106 30-31 0.47622052249830205 1106 32-33 0.5275252129911436 1106 34-35 0.5614579797105534 1106 36-37 0.45171365167794164 1106 38-39 0.27545835108096384 1106 40-41 0.5445707590559223 1106 42-43 0.45525435528414704 1106 44-45 0.4368889363692432 1106 46-47 0.500919407956701 1106 48-49 0.41303577434373295 1106 50-51 0.4898731073389797 1106 52-53 0.5550678177174788 1106 54-55 0.7279116654658253 1106 56-57 0.6729900282649837 1106 58-59 0.7144633274256833 1106 60-61 0.7585669139933842 1106 62-63 0.6152348767452267 1106 64-65 0.5348675485291565 1106 66-67 0.6175710856611829 1106 68-69 0.734531198509579 1106 70-71 0.761357039399357 1106 72-73 0.6771116852725854 1106 74-75 0.715100816306304 1106 76-77 0.6618931355943509 1106 78-79 0.6749424527240855 1106 80-81 0.763538380585949 1106 82-83 0.8456427229500569 1106 84-85 0.8396038826763323 1106 86-87 0.8054997531323558 1106 88-89 0.9088614911153883 1106 90-91 0.8689776754922747 1106 92-93 0.9467928669897994 1106 94-95 1.055297421159441 1106 96-97 0.9671938685042427 1106 98-99 0.8302014051924616 1106 100-101 1.3301527445414756 1107 1 0.4070132521251182 1107 2 0.44807557441049894 1107 3 0.34668450300441833 1107 4 0.26383751961147084 1107 5 0.37795991644942717 1107 6 0.3600054189367654 1107 7 0.3424007594547547 1107 8 0.42584270926645473 1107 9 0.46406538015578036 1107 10-11 0.4886628759424738 1107 12-13 0.4870175737872344 1107 14-15 0.5067195220513767 1107 16-17 0.6148855162054687 1107 18-19 0.6853841450902962 1107 20-21 0.7018214861321681 1107 22-23 0.7482295778520154 1107 24-25 0.8023585237052444 1107 26-27 0.7568807303101863 1107 28-29 0.8694839025767251 1107 30-31 0.9264606029814288 1107 32-33 0.7991798482661778 1107 34-35 0.7817886850077329 1107 36-37 0.7451581067080966 1107 38-39 0.749454319770841 1107 40-41 0.8441010356646714 1107 42-43 0.8952192772283141 1107 44-45 0.8847182946989918 1107 46-47 0.9812520576993222 1107 48-49 0.9414157358905371 1107 50-51 0.7987129855087431 1107 52-53 0.7558809080623305 1107 54-55 0.9458726185302311 1107 56-57 0.9165700254491114 1107 58-59 1.027437265678806 1107 60-61 1.0979232050059764 1107 62-63 0.9650155738646831 1107 64-65 1.0519747664862038 1107 66-67 1.0215663682674645 1107 68-69 1.0469978901267112 1107 70-71 0.8780066948780956 1107 72-73 0.9271545248522557 1107 74-75 0.9317074858842176 1107 76-77 0.9906323643258439 1107 78-79 1.0930614651408987 1107 80-81 1.0551175661754613 1107 82-83 1.0097552185482286 1107 84-85 0.8278014565416854 1107 86-87 0.9551539312351025 1107 88-89 0.8153790251033506 1107 90-91 0.8903821828988896 1107 92-93 1.0588319431896238 1107 94-95 1.078644748213538 1107 96-97 1.067563345653273 1107 98-99 1.3128560089271595 1107 100-101 1.5015821102848363 1108 1 -0.06280835358401404 1108 2 0.09155280241297348 1108 3 0.06628989752963932 1108 4 0.13444788683339937 1108 5 0.052972195703752334 1108 6 0.019558036745380036 1108 7 -0.03987441783198875 1108 8 -0.10218352499924777 1108 9 -0.2248043184968722 1108 10-11 -0.08781023329312632 1108 12-13 0.031976177451426224 1108 14-15 0.12537949425050243 1108 16-17 0.20599292838070227 1108 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94-95 -3.8460271580776393 2316 96-97 -4.098029350418852 2316 98-99 -4.360789105591866 2316 100-101 -4.648940990603947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4850.0 3 1839.0 4 1868.0 5 2025.0 6 2251.0 7 2678.0 8 3171.0 9 3900.0 10 4964.0 11 5816.0 12 7299.0 13 8546.0 14 9614.0 15 11468.0 16 14223.0 17 18322.0 18 16804.0 19 11655.0 20 11790.0 21 12657.0 22 13701.0 23 15179.0 24 17068.0 25 19592.0 26 22671.0 27 26942.0 28 32263.0 29 38962.0 30 48038.0 31 59444.0 32 75648.0 33 99309.0 34 136785.0 35 197557.0 36 300922.0 37 430795.0 38 349224.0 39 53148.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36545937890431 18.046656259947483 14.502986753897023 23.084897607251182 2 15.687302500654745 19.55131207429512 40.113037011831324 24.648348413218812 3 19.531673384264096 23.947605607417678 29.326398772003436 27.19432223631479 4 13.301226589164333 16.145422040363673 36.37960209944646 34.173749271025535 5 14.559013352246506 36.53361926274021 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22.876675152321326 25.70006650918515 28.4778044706404 22.94545386785313 28-29 22.857854799182174 25.832100176456507 28.452691929300812 22.85735309506051 30-31 23.070514953797286 25.536929322591924 28.395742455491707 22.996813268119087 32-33 22.932365225821542 25.63655688893848 28.420112854030606 23.01096503120938 34-35 23.180641352666562 25.68893915470763 28.224496129364724 22.905923363261085 36-37 23.164698217752807 25.595689026401992 28.257891056464228 22.981721699380977 38-39 23.08267315958626 25.646092721433632 28.319357422657482 22.951876696322625 40-41 23.1210196713079 25.631136756908752 28.19531834387269 23.05252522791066 42-43 22.924339819215213 25.691154101868168 28.00740397256383 23.37710210635279 44-45 23.1044309595973 25.722150832323404 28.0058472030011 23.167571005078198 46-47 23.30581600241569 26.012860107865134 27.58480236829331 23.09652152142587 48-49 23.40242551245795 26.078487301248305 27.247311396411185 23.271775789882565 50-51 23.149809792190442 26.29698218898085 27.375567016121554 23.17764100270715 52-53 23.36776499667087 26.29233411510737 27.038479575725333 23.30142131249643 54-55 23.160106604739394 26.202068781798353 27.07344665163879 23.564377961823464 56-57 23.192716178717376 26.457821423579965 27.075326437657605 23.274135960045058 58-59 23.46943309788662 26.17531837387435 27.03570307434513 23.3195454538939 60-61 23.31422095331924 26.033488750324597 27.49378097121724 23.158509325138922 62-63 23.09303482907989 26.152736874320446 27.498614916755244 23.255613379844416 64-65 23.071424783213416 26.272685009773255 27.312672931168674 23.343217275844655 66-67 23.03393579853281 26.352846668377484 27.095904603384646 23.517312929705067 68-69 22.840820798193956 26.226485485416344 27.476267102850244 23.456426613539456 70-71 23.003189389703838 26.19465866263629 27.26955685338727 23.532595094272605 72-73 23.079593223319137 26.255218730380488 27.097621434325465 23.567566611974915 74-75 23.01155782796457 26.52012017751868 26.95913844904103 23.50918354547572 76-77 23.177797912431462 26.282054958046963 26.908961099657937 23.631186029863642 78-79 23.086578462553074 26.372822874128733 26.952226887041547 23.588371776276645 80-81 23.057225160317522 26.473483148699923 26.929135627797024 23.54015606318553 82-83 23.01122494698169 26.501030496729292 26.9273753696347 23.56036918665432 84-85 23.06301075962447 26.439289413231936 26.753680216841012 23.744019610302576 86-87 23.00158695907242 26.369137529204373 26.968052681019316 23.661222830703892 88-89 23.101064108199033 26.349601954270806 26.724404747820724 23.824929189709437 90-91 23.091606337285615 26.258337212959447 26.894460467486187 23.755595982268755 92-93 23.01967183652243 26.142114631609537 26.961280571743217 23.87693296012481 94-95 22.987147214990998 26.18352370248894 27.010320263611586 23.819008818908475 96-97 22.852804600804472 26.212495714222566 26.998331085415693 23.936368599557266 98-99 22.742674891031783 26.28142059330234 26.787816016195094 24.188088499470783 100-101 22.751217128447095 26.250814048784594 26.900642536714674 24.097326286053637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 196.0 2 190.5 3 242.5 4 220.5 5 245.5 6 417.0 7 860.0 8 1572.0 9 2015.5 10 1878.0 11 1767.0 12 1860.0 13 2019.0 14 2217.5 15 2362.0 16 2495.0 17 2623.0 18 2776.5 19 2959.0 20 3274.0 21 3745.0 22 4377.0 23 5178.5 24 6326.0 25 7804.0 26 9577.5 27 11736.0 28 13821.0 29 15978.0 30 18914.0 31 22405.0 32 25307.0 33 27909.0 34 31256.5 35 34045.0 36 37713.0 37 42064.0 38 45389.5 39 48018.5 40 50877.5 41 55266.5 42 59172.0 43 63659.0 44 69811.0 45 74215.0 46 77624.5 47 81389.5 48 85443.5 49 86095.0 50 83523.5 51 82296.5 52 81909.0 53 82137.0 54 81949.5 55 78490.5 56 72845.0 57 67167.0 58 61531.5 59 54667.0 60 46066.5 61 37301.0 62 29809.0 63 23575.0 64 17844.5 65 13266.5 66 9603.5 67 7210.5 68 5087.5 69 3206.5 70 2118.5 71 1361.5 72 902.0 73 606.5 74 392.5 75 224.0 76 153.0 77 94.5 78 47.0 79 33.5 80 32.0 81 26.0 82 21.0 83 15.0 84 10.5 85 10.5 86 10.5 87 7.5 88 5.5 89 4.0 90 2.5 91 3.5 92 2.5 93 0.5 94 1.5 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03678915704096688 2 0.028475763112228904 3 0.02360239425745148 4 0.04911591355599214 5 0.09789738022979368 6 0.11509750559959638 7 0.05843264813130193 8 0.08848508940242943 9 0.07200163592303517 10-11 0.07462943285453282 12-13 0.07228830467919856 14-15 0.06531269827922302 16-17 0.05293816363817051 18-19 0.06294768104087514 20-21 0.060033215353214125 22-23 0.03320579758892465 24-25 0.00910173300818726 26-27 0.003057800065742701 28-29 0.006736715769839389 30-31 0.005327261052036113 32-33 0.006091711068471788 34-35 0.006426157950662396 36-37 0.0055661516821722615 38-39 0.0029383547506746273 40-41 0.0065694923287440855 42-43 0.0037028047671103024 44-45 0.001982792230130033 46-47 0.0011466750246535132 48-49 3.3444688219060796E-4 50-51 0.0016244562849258102 52-53 0.0056617079342267205 54-55 0.011012858049276448 56-57 0.007190607967098071 58-59 0.005112259484913579 60-61 0.0017439015999938844 62-63 0.008289504865724355 64-65 0.002436684427388715 66-67 0.02097459732595384 68-69 0.044075321260119406 70-71 0.058695427824451696 72-73 0.003153356317797161 74-75 0.012923983090365635 76-77 0.020544594191708773 78-79 0.02451017865196884 80-81 0.07699445009288067 82-83 0.02725742089853455 84-85 0.011012858049276448 86-87 0.03064966784646786 88-89 0.04655978381353536 90-91 0.042140307156016604 92-93 0.04204475090396215 94-95 0.011084525238317293 96-97 0.01579067065199942 98-99 0.036765267977953264 100-101 0.07692278290383983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2093008.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.41039155790518 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49519694262217 41.077232263892384 2 13.159280836116357 14.320032458270576 3 4.566063332379748 7.453238813789263 4 2.064632894967883 4.493499369541354 5 1.2111037089529213 3.2948313510679847 6 0.744937314205163 2.431939857119829 7 0.5282616787633176 2.0120047350583903 8 0.3653784786432442 1.5904308871848487 9 0.270255891154875 1.3234255972710618 >10 1.4721276772819232 14.394589945762448 >50 0.08926564958160377 3.296361393175188 >100 0.03226232097910461 3.0619021032680966 >500 7.04728201000427E-4 0.2605511313498605 >1k 3.52364100500208E-4 0.35468697056455933 >5k 1.76182050250104E-4 0.6352731226841445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7581 0.36220597341242844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5673 0.27104530895247414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3435 0.16411786290353406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.777812602722971E-5 0.0 0.0 0.0 0.0 3 4.777812602722971E-5 0.0 0.0 0.0 0.0 4 4.777812602722971E-5 0.0 0.0 0.0 0.0 5 1.4333437808168912E-4 0.0 0.0 0.0 0.0 6 1.9111250410891884E-4 0.0 0.0 0.0 0.0 7 2.3889063013614854E-4 0.0 0.0 0.0 0.0 8 2.8666875616337824E-4 0.0 0.0 0.0 0.0 9 2.8666875616337824E-4 0.0 0.0 0.0 4.777812602722971E-5 10-11 3.105578191769931E-4 0.0 0.0 0.0 4.777812602722971E-5 12-13 4.300031342450674E-4 0.0 0.0 0.0 1.1944531506807427E-4 14-15 5.255593862995269E-4 0.0 0.0 0.0 1.4333437808168912E-4 16-17 5.972265753403713E-4 0.0 0.0 0.0 1.9111250410891884E-4 18-19 7.166718904084456E-4 0.0 0.0 0.0 1.9111250410891884E-4 20-21 8.122281424629051E-4 0.0 0.0 7.166718904084456E-5 1.9111250410891884E-4 22-23 8.600062684901348E-4 0.0 0.0 2.3889063013614854E-4 1.9111250410891884E-4 24-25 0.0010988968986262832 0.0 0.0 3.822250082178377E-4 1.9111250410891884E-4 26-27 0.0011227859616398982 0.0 0.0 6.688937643812159E-4 1.9111250410891884E-4 28-29 0.0012422312767079724 0.0 0.0 0.0014094547178032765 1.9111250410891884E-4 30-31 0.0012900094027352021 0.0 0.0 0.002627796931497634 2.150015671225337E-4 32-33 0.001313898465748817 0.0 0.0 0.004300031342450674 2.3889063013614854E-4 34-35 0.0017200125369802696 0.0 0.0 0.006306712635594322 2.627796931497634E-4 36-37 0.0021022375451981074 0.0 0.0 0.008934509567091955 2.8666875616337824E-4 38-39 0.0022455719232797964 0.0 0.0 0.014046769052005536 2.8666875616337824E-4 40-41 0.002269460986293411 0.0 0.0 0.018776803528701275 2.8666875616337824E-4 42-43 0.0024127953643751 0.0 0.0 0.02350683800539702 3.105578191769931E-4 44-45 0.0028905766246473973 0.0 0.0 0.027281309961548164 3.3444688219060796E-4 46-47 0.0033922469479333093 0.0 0.0 0.033134130399883804 3.583359452042228E-4 48-49 0.0035833594520422282 0.0 0.0 0.037601385183429786 4.300031342450674E-4 50-51 0.0035833594520422282 0.0 0.0 0.042092529029989376 4.5389219725868227E-4 52-53 0.0035833594520422282 0.0 0.0 0.05152870892036724 4.777812602722971E-4 54-55 0.0035833594520422282 4.777812602722971E-5 0.0 0.06712826706825774 4.777812602722971E-4 56-57 0.003726693830123917 4.777812602722971E-5 0.0 0.09340623638323409 4.777812602722971E-4 58-59 0.003726693830123917 4.777812602722971E-5 0.0 0.13222596378035822 4.777812602722971E-4 60-61 0.0037505828931375324 4.777812602722971E-5 0.0 0.17379293342404806 4.777812602722971E-4 62-63 0.003774471956151147 4.777812602722971E-5 0.0 0.1997364558568338 6.211156383539862E-4 64-65 0.003774471956151147 4.777812602722971E-5 0.0 0.23224947061836362 6.211156383539862E-4 66-67 0.004013362586287296 4.777812602722971E-5 0.0 0.2964154938729331 6.211156383539862E-4 68-69 0.004108918838341755 4.777812602722971E-5 0.0 0.39022784432739865 6.211156383539862E-4 70-71 0.004491143846559593 4.777812602722971E-5 0.0 0.48048072439283557 6.211156383539862E-4 72-73 0.004730034476695741 4.777812602722971E-5 0.0 0.5871215016856124 6.688937643812159E-4 74-75 0.004777812602722971 4.777812602722971E-5 0.0 0.689486136698952 6.688937643812159E-4 76-77 0.004777812602722971 4.777812602722971E-5 0.0 0.781029026167124 6.688937643812159E-4 78-79 0.004801701665736586 4.777812602722971E-5 0.0 0.8608184966325977 6.688937643812159E-4 80-81 0.005518373556145031 4.777812602722971E-5 0.0 0.9305267825063258 6.688937643812159E-4 82-83 0.005590040745185876 4.777812602722971E-5 0.0 1.0041528747142867 7.166718904084456E-4 84-85 0.00570948606025395 4.777812602722971E-5 0.0 1.1675540657274124 7.166718904084456E-4 86-87 0.005781153249294795 4.777812602722971E-5 0.0 1.428088186953896 7.644500164356754E-4 88-89 0.005781153249294795 4.777812602722971E-5 0.0 1.7574228096595905 8.122281424629051E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5745 0.0 39.225483 1 GTATCAA 9150 0.0 38.241657 1 TATCAAC 11545 0.0 30.2709 2 ATCAACG 11600 0.0 30.127375 3 TCAACGC 11575 0.0 30.110289 4 CAACGCA 11800 0.0 29.374973 5 AACGCAG 12110 0.0 28.579647 6 ACGCAGA 13475 0.0 25.502058 7 CGCAGAG 13635 0.0 24.99425 8 GCAGAGT 14115 0.0 22.997719 9 GAGTACT 6460 0.0 22.844372 12-13 GTACATG 8860 0.0 22.644289 1 TACATGG 9075 0.0 21.481728 2 AGAGTAC 11615 0.0 21.297354 10-11 GTACTTT 6920 0.0 21.222538 14-15 ACATGGG 9185 0.0 20.137354 3 AGTACTT 6815 0.0 20.015488 12-13 ATTCGCG 265 0.0 19.726273 80-81 CTATACG 125 0.005997694 19.019186 4 CAGAGTA 13855 0.0 18.83172 10-11 >>END_MODULE